Importance of Diversity in the Oral Microbiota including Candida Species Revealed by High-Throughput Technologies by Tamaki Cho, Jun-ichi Nagao, Rieko Imayoshi, Yoshihiko Tanaka

“…Taking advantage of high-throughput technologies, deep sequencing of the human microbiome has revealed commensal bacteria independent of the ability to culture them. …”
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Overview on Cryptosporidium bovis and Its Effect on Calves in Some Governorates in Egypt by Amer R. Abdelaziz, Amin Tahoun, Hanem El-Sharkawy, Moustafa M. Abd El-Salam, Mohammed Alorabi, Ahmed M. El-Shehawi, Rasha A. El Meghanawy, Essam E Toukhy, Ahmed M. Abd El-Salam, Shimaa S. G. Sorour

“…The present study was conducted to elucidate the prevalence of Cryptosporidium bovis in suckling and weaned cattle calves (Bubalus bubalis) from different governorates in northern, middle, and southern Egypt, such as Behera, Menofia, Qaliubiya, Assiut, and Sohag; result revealed that from the overall examined fecal samples (n = 825), the overall prevalence was 7.27%, the highest significant infection rate was in young suckling calves less than one month (8.2%), and seasonally, winter season has the highest significant level (11.24%), but sex and locality were of no significant effect on the prevalence of infection in this study. Gene sequencing and phylogenetic analysis of the 18SSU-rRNA gene of the local bovine isolate were performed, and it was found that C. bovis genotype was highly similar to human isolate, which provoke the zoonotic transmission of bovine isolate to humans and identified as a potential source for human cryptosporidiosis infection in Egypt.…”
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Alternative 3′ UTR polyadenylation is disrupted in the rNLS8 mouse model of ALS/FTLD by Randall J. Eck, Paul N. Valdmanis, Nicole F. Liachko, Brian C. Kraemer

“…Here, we identify significant disruptions to 3` UTR polyadenylation in the ALS/FTLD-TDP mouse model rNLS8 that correlate with changes in gene expression and protein levels through the re-analysis of published RNA sequencing and proteomic data. A subset of these changes are shared with TDP-43 knock-down mice suggesting depletion of endogenous mouse TDP-43 is a contributor to polyadenylation dysfunction in rNLS8 mice. …”
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Pathogenesis of Helicobacter pylori-Related Gastroduodenal Diseases from Molecular Epidemiological Studies by Yoshio Yamaoka

“…Because genome wide analyses using whole-genome sequencing technology give a broad view of the genome of H. pylori, we hope that next-generation sequencers will enable us to efficiently investigate novel virulence factors.…”
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Genome Assembly and Genome Annotation of Leishmania martiniquensis Isolated from a Leishmaniasis Patient in Thailand by Songtham Anuntakarun, Atchara Phumee, Vorthon Sawaswong, Kesmanee Praianantathavorn, Witthaya Poomipak, Rungrat Jitvaropas, Padet Siriyasatien, Sunchai Payungporn

“…This species was isolated in culture and subjected to high-throughput whole-genome sequencing. A total of 30.8 Mb in 36 chromosomes of the whole genome was assembled, annotated, and characterized. …”
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Review of Biological Network Data and Its Applications by Donghyeon Yu, MinSoo Kim, Guanghua Xiao, Tae Hyun Hwang

“…Various types of large-scale biological datasets have been collected and analyzed with high-throughput technologies, including DNA microarray, next-generation sequencing, and the two-hybrid screening system, for this purpose. …”
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Fecal microbiota changes associated with pathogenic and non-pathogenic diarrheas in foals by Yijun Shi, Elizabeth A. Maga, Michael J. Mienaltowski

“…As a result, significantly higher alpha diversity was seen in the non-pathogenic samples than in pathogenic ones. Sequencing of the V4 domains of bacterial 16 S rRNA genes demonstrated that non-pathogenic samples had more alpha diversity. …”
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In vivo regulation of the monocyte phenotype by Mycobacterium marinum and the ESX-1 type VII secretion system by Kristina Munke, Line Wulff, Julia Lienard, Fredric Carlsson, William W. Agace

“…In the current study we use this model, in combination with scRNA sequencing, to assess the impact of M. marinum infection on the transcriptional profile of infiltrating Ly6C⁺MHCII⁺ monocytes in vivo. …”
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Emerging Paradigms in Genomics-Based Crop Improvement by Abhishek Bohra

“…Next generation sequencing platforms and high-throughput genotyping assays have remarkably expedited the pace of development of genomic tools and resources for several crops. …”
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Rare Suprasellar Chordoid Meningioma with INI1 Gene Mutation by Imad Fadl-Elmula, Rania Giha, Sawsan AH Deaf, Lamyaa Ahmed El Hassan, Ahmed M. Hassan, Mohamed AR Arbab

“…Genomic DNA was extracted and mutation analysis for INI1 gene using primer of exon 4, 5, 7, and 9 showed mutation involving exon 9. DNA sequencing showedheterozygosity C­­­­­­­­­­­­­­­­­­-T mutation in exon 9 of INI1 gene leading to change of amino acid serine to phenylalanine at (codon 63). …”
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Reflections on the US FDA's Warning on Direct-to-Consumer Genetic Testing by Seon-Hee Yim, Yeun-Jun Chung

“…This action was also significant, since it reflected the FDA's attitude towards medical application of next-generation sequencing techniques. In this review, we followed up on the FDA-23andMe incident and evaluated the problems and prospects for DTC genetic testing.…”
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Unravelling the transcriptomic dynamics of Hyphopichia pseudoburtonii in co-culture with Botrytis cinerea. by Evelyn Maluleke, Neil Paul Jolly, Hugh-George Patterton, Mathabatha Evodia Setati

“…This study employed RNA sequencing to assess the temporal changes in H. pseudoburtonii Y963 gene expression patterns when co-cultivated with Botrytis cinerea. …”
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The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus by Stephen D. Simpson, Jordan S. Ramsdell, Winsor H. Watson III, Christopher C. Chabot

“…In this study, horseshoe crabs were collected during the day and night as well as high and low tides, and their muscle and central nervous system tissues were processed for genome and transcriptome sequencing, respectively. The genome assembly resulted in 7.4×105 contigs with N50 of 4,736, while the transcriptome assembly resulted in 9.3×104 contigs and N50 of 3,497. …”
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Metabolic Analysis of the Mode of Action and Mode of Resistance for Novobiocin in Staphylococcus aureus by Weile Xie, Dan Luo, Zhe Wang

“…Through whole genome sequencing, three mutations of S. aureus in gyrB, potB, and fpgS were identified. …”
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Recurrent Rhabdomyolysis in a Medical Cadet during Military Training as a Rare Initial Presentation in Calpainopathy by Sethapong Lertsakulbunlue, Boonsub Sakboonyarat, Piradee Suwanpakdee, Boonchai Boonyawat

“…The diagnosis was confirmed by identifying a compound heterozygous of a novel frameshift, c.606dup (p.Ala203CysfsTer9), a mutation in exon 4, and a missense, c.956C > T (p.Pro319Leu), a mutation in exon 7 of the CAPN3 gene, via whole exome sequencing. This case highlights the necessity of diagnostic investigation in individuals who have persistent high CK levels during the rhabdomyolysis episodes and possibly CK screening prior to military training to preemptively identify and mitigate complications from undiagnosed muscular dystrophies in military personnel in the future.…”
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Genetic investigation of patients with autosomal recessive ataxia and identification of two novel variants in the SQSTM1 and SYNE1 genes by Diana Mokhtari, Mohammad Jahanpanah, Nasim Jabbari, Hamed Azari, Sana Davarnia, Haleh Mokaber, Sara Arish, Rasol Molatefi, Vahid Abbasi, Behzad Davarnia

“…We identified two novel pathogenic variants of the SQSTM1 and SYNE1 genes via whole-exome sequencing in patients with ARCAs.…”
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The Expression Pattern and Functional Analysis of Extracellular Vesicle Long Non-Coding RNAs from Uterine Fluid During Implantation in Pig by Yijun Shang, Qiuping Zhang, Yue Ding, Yongzhong Wang, Shengchen Gu, Xupeng Zang, Zheng Xu, Sixiu Huang, Zicong Li, Zhenfang Wu, Ting Gu, Linjun Hong, Gengyuan Cai

“…In the present study, high-throughput sequencing and biological tools were applied to analyse lncRNAs in UF-EVs on days 9, 12, and 15 of pregnancy to identify key regulatory lncRNAs in UF-EVs during the porcine embryonic implantation period and to explore their expression patterns and functional roles. …”
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miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken by Qingqing Geng, Cai Hu, Ziduo Zhao, Zhe Wang, Fufu Cheng, Jing Chen, Qisheng Zuo, Yani Zhang

“…In this study, miR-1458, which was differentially expressed during SSCs formation, was selected by transcriptomic sequencing. We found that miR-1458, inhibited in an in vitro SSCs induction model,significantly upregulated the expression of germline marker genes (Cvh and integrin β1). …”
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IFN-γ reprograms cardiac microvascular endothelial cells to mediate doxorubicin transport and influences the sensitivity of mice to doxorubicin-induced cardiotoxicity by Haoyu Ji, Wenya Ma, Xu Liu, Hongyang Chen, Yining Liu, Zhongyu Ren, Daohong Yin, Ao Cai, Zizhen Zhang, Xin Wang, Wei Huang, Leping Shi, Yanan Tian, Yang Yu, Xiuxiu Wang, Yang Li, Yu Liu, Benzhi Cai

“…Through surface-enhanced Raman spectroscopy and single-cell RNA sequencing, we explored the mechanisms underlying DIC phenotypic variations. …”
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Lower respiratory tract microbiome dysbiosis impairs clinical responses to immune checkpoint blockade in advanced non‐small‐cell lung cancer by Yong Zhang, Xiang‐Xiang Chen, Ruo Chen, Ling Li, Qing Ju, Dan Qiu, Yuan Wang, Peng‐Yu Jing, Ning Chang, Min Wang, Jian Zhang, Zhi‐Nan Chen, Ke Wang

“…Methods During this study, 26 bronchoalveolar lavage fluids (BALF) from advanced NSCLC participants who received immune checkpoint inhibitor monotherapy were performed 16S rRNA sequencing and untargeted metabolome sequencing to identify differentially abundant microbes and metabolic characteristics. …”
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