Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems by Keisuke Fukunaga, Masumi Taki

“…In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.…”
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Transposable Elements: No More 'Junk DNA' by Yun-Ji Kim, Jungnam Lee, Kyudong Han

“…Since the advent of whole-genome sequencing, transposable elements (TEs), just thought to be 'junk' DNA, have been noticed because of their numerous copies in various eukaryotic genomes. …”
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Genome-Based Virus Taxonomy with the ICTV Database Extension by Shinduck Kang, Young-Chang Kim

“…Due to the development of next-generation sequencing and the accompanying bioinformatics analysis pipelines, a reconstruction of the classification system has been proposed. …”
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Mathematical analysis and simulations involving chemotherapy and surgeryon large human tumours under a suitable cell-kill functional response by Diego Samuel Rodrigues, Paulo Fernando de Arruda Mancera

“…Dosage and frequency of treatment schedulesare important for successful chemotherapy.However, in this work we argue that cell-kill response and tumoralgrowth should not be seen as separate and therefore are essential in a mathematical cancer model.This paper presents a mathematical model for sequencing of cancer chemotherapy andsurgery. Our purpose is to investigate treatments for large human tumoursconsidering a suitable cell-kill dynamics. …”
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Unraveling the metabolic landscape of Exophiala spinifera strain FM: Model reconstruction, insights into biodesulfurization and beyond. by Hamta Babaei Naeij, Zahra Etemadifar, John Kilbane, Mohammad Hossein Karimi-Jafari, Sepideh Mofidifar

“…However, the specific pathway and enzymes involved in this process remain unclear due to limited genome sequencing and metabolic understanding of E. spinifera. …”
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Microevolution during Chronic Infection May Lead T. asahii to Coexist with the Host by Gen Ba, Xuelian Lv, Xin Yang, Wenling Wang, Junhong Ao, Rongya Yang

“…Two T. asahii strains were isolated from a chronic trichosporonosis patient between a 15-year interval, and the microevolutionary changes were compared by the immune response of dendritic cell (DC), mice survival model, and transcriptome sequencing analysis. Results. Compared with the primary T. asahii strain, the microevolved strain induced much lower expression of TNF-α by mice bone marrow-derived DC and had a much superior survival rate, a total of 2212 significantly differentially expressed genes were identified in the microevolved strain, and functional analysis showed significance in the downregulated transcription and metabolic process, especially the valine, leucine, and isoleucine degradation pathways, which were associated with pathogenicity and virulence; hence, the results were highly consistent with the decreased immunogenicity and virulence of the microevolved strain. …”
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RNA-Binding Proteins in Amyotrophic Lateral Sclerosis and Neurodegeneration by Scott E. Ugras, James Shorter

“…In a recent study, Couthouis et al. screened for protein candidates that were also involved in RNA processing, contained a predicted prion domain, shared other phenotypic similarities with TDP-43 and FUS, and identified TAF15 as a putative ALS gene. Subsequent sequencing of ALS patients successfully identified ALS-linked mutations in TAF15 that were largely absent in control populations. …”
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Extensive Mycobacterium abscessus Pneumonia in an Immunocompetent Infant with No Underlying Lung Pathology by Morouge M. Alramadhan, James R. Murphy, Michael L. Chang

“…We describe our complete evaluation including immunodeficiency evaluation incorporating whole-exome sequencing, describe our antibiotic selection and treatment duration given complicated susceptibility pattern of the M. abscessus isolate, and review literature for nontuberculous mycobacterial pulmonary disease in immunocompetent children. …”
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Protein C Deficiency Caused by a Novel Mutation in the PROC Gene in an Infant with Delayed Onset Purpura Fulminans by Mariam S. Al Harbi, Ayman W. El-Hattab

“…Protein C level was less than 10% and PROC gene sequencing identified a novel homozygous missense mutation, c.1198G>A (p.Gly400Ser). …”
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Microbiome and Colorectal Cancer in Humans: A Review of Recent Studies by Hiroyuki Takamaru, Cynthia Tsay, Satoshi Shiba, Shinichi Yachida, Yutaka Saito

“…In recent years, metagenomic techniques such as shotgun sequencing and genome-wide association studies focused on understanding the role of the microbiota and the metabolome on early CRCs and colon carcinogenesis to determine if there are modifiable or intervenable targets for CRC. …”
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Mapping transcriptomic profiles of seminal vesiculitis and experimental verification by Chenkui Miao, Shaobo Zhang, Jiajin Wu, Meiling Bao, Zengjun Wang, Bianjiang Liu

“…Methods: In this study, we utilized a next-generation sequencing technology to demonstrate transcriptomic landscapes of clinical specimens from seminal vesiculitis patients. …”
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Muscular Endurance Training: Bridging Gym Practices with Science Recommendations by P. Prieto-Gonzalez, P. Sagat

“…An important percentage of study participants did not exercise according to the recommended guidelines regarding exercise sequencing, intensity, repetitions, rest periods, and training supervision. …”
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Measuring Fluid Intelligence in Healthy Older Adults by Mohammed K. Shakeel, Vina M. Goghari

“…Raven’s Advanced Progressive Matrices (RAPM) were used to measure fluid intelligence, Digit Span Sequencing (DSS) was used to measure working memory, Trail Making Test (TMT) was used to measure cognitive flexibility, Design Fluency Test (DFT) was used to measure creativity, and Tower Test (TT) was used to measure planning. …”
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Cutaneous Disease as Sole Clinical Manifestation of Protothecosis in a Boxer Dog by Emmanouil I. Papadogiannakis, Emmanouil N. Velonakis, Gregory K. Spanakos, Alexander F. Koutinas

“…Cytology and histopathology showed pyogranulomatous inflammation along with organisms containing round sporangia with spherical sporangiospores. PCR and sequencing identified the causal organism as Prototheca wickerhamii. …”
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Postural Dysfunction During Standing and Walking in Children With Cerebral Palsy: What are the Underlying Problems and What New Therapies Might Improve Balance? by Marjorie Hines Woollacott, Anne Shumway-Cook

“…Children with CP show (a) crouched posture, contributing to decreased ability to recover balance (longer time/increased sway); (b) delayed responses in ankle muscles; (c) inappropriate muscle response sequencing; (d) increased coactivation of agonists/antagonists. …”
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Expert Consensus on Tiered Detection of Newly Emerging and Outbreak Respiratory Infectious Pathogens (2024) by Chinese Hospital Association Clinical Microbiology Laboratory Specialized Committee

“…This consensus analyzes the major characteristics of newly emerging and outbreak respiratory infections, considers patient population characteristics, and proposes a three-tiered detection strategy that includes traditional microbiological morphology, culture, immunology, molecular biology, and metagenomic sequencing methods. It defines the target pathogens and recommends detection methods at different stages, providing guidance and reference for improving the diagnostic capacity for newly emerging and outbreak respiratory infectious diseases.…”
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Heterogeneity in NK Cell Subpopulations May Be Involved in Kidney Cancer Metastasis by Zhengfang Liang, Fengwei Nong, Jingjie Zhao, Dalong Wei, Qianli Tang, Jian Song, Lingzhang Meng

“…In this study, natural killer (NK) cell populations were isolated using signature genes from the single-cell sequencing data of clear cell renal cell carcinoma (ccRCC) and normal kidney tissues and divided into three subpopulations according to the differences in gene expression profiles: NK(GZMH), NK(EGR1), and NK(CAPG). …”
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Single-cell RNA-seq data augmentation using generative Fourier transformer by Nima Nouri

“…Abstract Single-cell RNA sequencing (scRNA-seq) provides a powerful tool for dissecting cellular complexity and heterogeneity. …”
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Knockdown-resistant mutations in head lice (Pediculus humanus capitis) collected from schoolchildren in Riyadh, Saudi Arabia by Imtinan H. Alghashmari, Noha T. Zelai

“…To do that, Sanger sequencing was employed to find the frequency of mutated alleles in the voltage-sensitive sodium channel gene (VSSC). …”
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Is the Goat a New Host for the G3 Indian Buffalo Strain of Echinococcus granulosus? by Pietro Calderini, Simona Gabrielli, Gabriella Cancrini

“…Specific PCR followed by DNA sequencing of the mitochondrial cox1 gene revealed for all the isolates 99.5% identity to the reference strain G3 genotype and 99.3% and 99.1% to G2 and G1, respectively. …”
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