Bioadsorptive Removal of the Pollutant Zn(II) from Wastewater by Delftia tsuruhatensis Biomass by Samiah Hamad Al-Mijalli

“…Selectively, the bacterium strain SA-101 was selected as the most zinc-resistant and identified by 16S rRNA sequencing as Delftia tsuruhatensis SA-101. D. tsuruhatensis SA-101 has been assigned the accession number MW629784 in the GenBank database. …”
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Investigation and Identification of Fungal Diseases of <i>Aloe barbadensis</i> in China by Guohui Zhang, Qingjia Wan, Xiangyang Li, Jie Deng

“…The pathogenic fungi of wild and cultivated species of <i>A. barbadensis</i> were isolated by a tissue separation method, and DNA sequencing was carried out by using the sequence analysis of the ribosomal rDNA-ITS region, and the pathogenic fungi were classified and identified by finally combining morphological observations. …”
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Integrating representation learning, permutation, and optimization to detect lineage-related gene expression patterns by Hannah M. Schlüter, Caroline Uhler

“…Abstract Recent barcoding technologies allow reconstructing lineage trees while capturing paired single-cell RNA-sequencing (scRNA-seq) data. Such datasets provide opportunities to compare gene expression memory maintenance through lineage branching and pinpoint critical genes in these processes. …”
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LARP3 inhibits the apoptosis of hepatocellular carcinoma via the ROS/PI3K/c-Fos axis. by Lin Zhu, Qianqian Meng, Weiyi Qian, Weiting Shao, Yuyue Lu, Shuai Jin, Afei Zhang, Shuang G Yan, Jingtao Lu

“…Finally, through biological means such as RNA sequencing, flow cytometry, western blotting, and the construction of a subcutaneous tumorigenesis model in nude mice, we concluded that inhibition of HCC apoptosis by LARP3 is related to LARP3 negatively regulating ROS level and inhibiting the PI3K/c-Fos/apoptosis axis. …”
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Role of plasmids in antibiotic resistance in clinical infections and implications for epidemiological surveillance: a review by Ayodele Oluwaseun Ajayi, Olajide Joseph Akinjogunla, Adebowale Toba Odeyemi, Akinyomade Oladipo Owolabi

“…Recent technologies like replicon typing and whole genome sequencing (WGS) have become the gold standard for molecular epidemiology of plasmids for the detection and control of epidemics in clinical settings. …”
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Genotoxicity Studies of Titanium Dioxide Nanoparticles (TiO2NPs) in the Brain of Mice by Hanan R. H. Mohamed, Nahed A. Hussien

“…Moreover, Comet assay revealed the apoptotic DNA fragmentation, while PCR-SSCP pattern and direct sequencing showed point mutation of Presenilin 1 gene at exon 5, gene linked to inherited forms of the Alzheimer’s disease. …”
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Application of Spatial Transcriptomics in Digestive System Tumors by Bowen Huang, Yingjia Chen, Shuqiang Yuan

“…This review also discusses the importance of combining spatial transcriptomics with single-cell RNA sequencing (scRNA-seq), artificial intelligence, and machine learning in digestive system cancer research.…”
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Identification of Novel Mutations in Spatacsin and Apolipoprotein B Genes in a Patient with Spastic Paraplegia and Hypobetalipoproteinemia by Leema Reddy Peddareddygari, Raji P. Grewal

“…This clinical study provides support for the use of whole exome sequencing as a diagnostic tool for identification of mutations in conditions with complex presentations.…”
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Case Report: A case of Tatton-Brown–Rahman syndrome featuring mitral annular disjunction and mitral valve prolapse due to a novel mutation site in the DNMT3A gene by Zhong-jiao Xu, Ru-ming Shen, Wu-ming Hu, Lin-chun Lv, Zhen-hua Shi, Li Lin

“…These symptoms suggested a possible genetic disorder. High-throughput sequencing revealed a specific mutation in the DNMT3A gene (NM_175629.2:c.2408 + 1G &gt; A) associated with Tatton-Brown–Rahman syndrome. …”
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Influence of Polymorphism on Glycosylation of Serum Amyloid A4 Protein by Toshiyuki Yamada, Jyunji Sato, Kazuhiko Kotani, Masafumi Tanaka

“…In 55 healthy subjects, SAA4 polymorphism was analyzed by PCR combined direct sequencing and its glycosylation status was analyzed by immunoblotting. …”
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Wilson disease (novel ATP7B variants) with concomitant FLNC-related cardiomyopathy by Takeshi Imai, Satomi Mitsuhashi, Kenji Isahaya, Soichiro Shibata, Yosuke Kawai, Yosuke Omae, Katsushi Tokunaga, NCBN Controls WGS Consortium, Yoshihisa Yamano

“…Abstract We report a case of Wilson disease (WD) with dilated cardiomyopathy in which whole-genome sequencing (WGS) revealed the rare co-occurrence of two novel compound heterozygous ATP7B pathogenic variants (NM_001005918.3:c.2250del/p.N751Tfs*9 and c.3496C>T/p.L1166F) and a known FLNC pathogenic variant. …”
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An Anaplastic Lymphoma Kinase-positive Inflammatory Myofibroblastic Tumor with Rapidly Acquired Resistance to First-line Anaplastic Lymphoma Kinase Inhibitor: A Case and Literature... by Shu-Cheng Wu, Hsing-Wu Chen

“…Repeated biopsy and next-generation sequencing (NGS) showed ALK:c.3604G>A; p.(Gly1202Arg), which is a common mechanism of drug resistance in ALK fusion-positive non-small cell lung cancer. …”
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Learning predictive signatures of HLA type from T-cell repertoires. by María Ruiz Ortega, Mikhail V Pogorelyy, Anastasia A Minervina, Paul G Thomas, Thierry Mora, Aleksandra M Walczak

“…Immune repertoire sequencing has produced large numbers of datasets, however the HLA type of the corresponding donors is rarely available. …”
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Preliminary investigation on the establishment of a new meibomian gland obstruction model and gene expression by Ming Sun, Huanmin Cheng, Zheng Yang, Jiangqin Tang, Shengshu Sun, Zhanglin Liu, Shaozhen Zhao, Lijie Dong, Yue Huang

“…Changes in the histological structure of the meibomian gland (MG) were observed by HE staining, Oil Red O staining and immunofluorescence staining (collagen IV). RNA sequencing was used to detect differentially expressed genes in MGO and normal rats, which were validated by qPCR. …”
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Contribution of cuproptosis and immune-related genes to idiopathic pulmonary fibrosis disease by Chengji Jin, Jia Li, Qiaoyu Li, Lipeng Zhang, Shaomao Zheng, Qiong Feng, Yongjie Li, Yu Zheng, Qiuli Nie, Jin Liang, Jing Wang, Jing Wang

“…Finally, our single-cell sequencing data revealed that in patients with IPF, CXCL12 was highly expressed in the endothelial cell subset (ECs), while SPP1 exhibited high expression in multiple cellular populations. …”
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Emergence and clonal dissemination of KPC-2- and NDM-1-coharboring Citrobacter freundii in China with an IncR plasmid by Juanjuan Zhou, Minghui Zhen, Kaijie Gao, Lu Xu, Dongyu Zhang, Junmei Yang

“…Comprehensive characterization, including antimicrobial susceptibility testing, plasmid conjugation, whole-genome sequencing, and comparative genomics, was conducted for these clinical strains. …”
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Association of pituitary neuroendocrine tumors and neurofibromatosis type 1: assessing causation versus coincidence. Case report by Mercedes Aguilar-Soto, Julia M. Zuarth-Vázquez, Laura Leyva-Figueroa, Karla Zarco-Ávila, Armando Gamboa-Domínguez, Aldo Eguiluz-Melendez, Laura C. Hernández-Ramírez

“…Genetic testing was carried out by means of targeted next generation sequencing (NGS) and Sanger sequencing in blood and tumor DNA, respectively. …”
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Genetic Variation Analysis of Hsp101 Gene in Common Wild Rice and Asian Cultivated Rice Germplasm Resources by Yue LIU, Liqun JIANG, Shuwei LYU, Bingrui SUN, Chen LI, Qing LIU, Hang YU

“…In addition, three Xian or Geng rice specific variations were validated by using PCR amplification and Sanger sequencing. It was found that the specific variation of Hsp101 gene existed in Xian rice and Geng rice.…”
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Increased interferon I signaling, DNA damage response and evidence of T-cell exhaustion in a patient with combined interferonopathy (Aicardi-Goutières Syndrome, AGS) and cohesinopa... by Sorina Boiu, Nikolaos Paschalidis, George Sentis, Theodora Manolakou, Andrianos Nezos, Manolis Gialitakis, Maria Grigoriou, Erato Atsali, Melpomeni Giorgi, Argirios Ntinopoulos, Clio Mavragani, Periklis Makrythanasis, Dimitrios T. Boumpas, Aggelos Banos

“…Results Next generation exome sequencing confirmed a homozygous SAMHD1 gene mutation and a hemizygous non-synonymous mutation on SMC1A gene, responsible for the AGS and CdLS, respectively. …”
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Transcriptome signature in the blood of neuromyelitis optica spectrum disorder under steroid tapering by Ryohei Yamamura, Makoto Kinoshita, Yoshiaki Yasumizu, Yoshiaki Yasumizu, Yoshiaki Yasumizu, Tomohiro Yata, Keigo Kihara, Daisuke Motooka, Daisuke Motooka, Naoyuki Shiraishi, Yasuko Sugiyama, Shohei Beppu, Hisashi Murata, Naoshi Koizumi, Itsuki Sano, Toru Koda, Tatsusada Okuno, Hideki Mochizuki

“…In this study, we collected peripheral blood mononuclear cells (PBMCs) from NMOSD patients during steroid tapering and performed bulk RNA sequencing to analyze changes in immune dynamics caused by steroid reduction.MethodsPBMCs were collected at 3–5 timepoints from 10 NMOSD patients at our hospital (including one relapse case), and bulk RNA sequencing was performed. …”
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