Identification of novel fructo-oligosaccharide bacterial consumers by pulse metatranscriptomics in a human stool sample by Catherine Prattico, Emmanuel Gonzalez, Lharbi Dridi, Shiva Jazestani, Kristin E. Low, D. Wade Abbott, Corinne F. Maurice, Bastien Castagner

“…We then performed metatranscriptomics, paired with whole metagenomic sequencing, and 16S amplicon sequencing. The short incubation was sufficient to cause induction of genes involved in carbohydrate metabolism, like carbohydrate-active enzymes (CAZymes), including glycoside hydrolase family 32 genes, which hydrolyze fructan polysaccharides like FOS and inulin. …”
Get full text

Association of FMO3 Variants with Blood Pressure in the Atherosclerosis Risk in Communities Study by Tyler S. Bryant, Priya Duggal, Bing Yu, Alanna C. Morrison, Tariq Shafi, Georg Ehret, Nora Franceschini, Eric Boerwinkle, Josef Coresh, Adrienne Tin

“…These associations were not significant with additional samples: E158K in a meta-analysis of SBP of African ancestry (N=30,841, p=0.43) that included ARIC participants and the two low frequency variants in an independent ancestry-specific exome sequencing study of blood pressure (rs200985584, p=0.94; rs75904274, p=0.81). …”
Get full text

Association between ABCB4 variants and intrahepatic cholestasis of pregnancy by Dekun Zhang, Yuhong Li, Shufeng He, Jing Shu, Tiechen Li, Qing Sun

“…DNA was extracted from peripheral blood of ICP patients and healthy pregnant women, 27 exons were sequencing by Sanger sequencing. Polymerase chain reaction (PCR) was used to amplify those exons. …”
Get full text

Rare Somatic MEN1 Gene Pathogenic Variant in a Patient Affected by Atypical Parathyroid Adenoma by Luigia Cinque, Flavia Pugliese, Celeste Clemente, Stefano Castellana, Maria Pia Leone, Danilo de Martino, Teresa Balsamo, Claudia Battista, Tommaso Biagini, Paolo Graziano, Marco Castori, Alfredo Scillitani, Vito Guarnieri

“…CDC73 gene was screened at germline and somatic levels with no results. Whole exome sequencing performed on DNA extracted from blood leukocytes and tumour tissue revealed a somatic MEN1 gene heterozygous variant, c.912+1G > A, of the splicing donor site of exon 6. …”
Get full text

Characterization of subcutaneous and visceral de-differentiated fat cells by Yan Li, Houyu Zhang, Carlos F. Ibáñez, Meng Xie

“…Methods: Here, we used in vitro ''ceiling culture'', single cell/nucleus RNA sequencing, epigenetic anaysis and genetic lineage tracing to address these unknowns. …”
Get full text

Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…Bisulfite sequencing result showed that SOST gene promoter was slightly demethylated in the OPF group, as compared with non-OPF group. …”
Get full text

Ly6E on tumor cells impairs anti-tumor T-cell responses: a novel mechanism of tumor-induced immune exclusion by Lan Hailin, Chen Yiting, Wu Yue, Li Lijun, Zhang Renlu, Chen Yunhan, Zhu Yanyang, Zhang Qiuyu

“…On day 7 after tumor implantation, tumor tissues were separated for flow cytometric assay, bulk RNA sequencing and single-cell RNA sequencing (ScRNA-seq). …”
Get full text

Integrated analysis of ATAC-seq and RNA-seq reveals ADSCP2 regulates oxidative phosphorylation pathway in hypertrophic scar fibroblasts by Qian Li, Zhe Quan, Ling Chen, Yiliang Yin, Xin Chen, Jingyun Li

“…This was achieved through the integrated analysis of assay for transposase accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq). …”
Get full text

Novel pyroptosis-immune-related lncRNA signature exhibits a distinct immune cell infiltration landscape in breast cancer by Dedi Kong, Dedi Kong, Hongju Cheng, Meihong Wang

“…The Wilcoxon test was used to reveal differences in immune scores, cell types, functions, and checkpoint genes between these groups. Single-cell sequencing data from GSE176078 were used to validate the immune cell infiltration landscape of the identified lncRNA signatures.ResultsWe identified a six-lncRNA pyroptosis-immune signature comprising MAPT.AS1, CTA.384, D8.34, RP11.561, I11.3, HID1.AS1, AC097713.3, and USP2.AS1. …”
Get full text

Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro by Xiangyu Huang, Wei Qiu, Yuhua Pan, Jianjia Li, Zhao Chen, Kaiying Zhang, Yifei Luo, Buling Wu, Wenan Xu

“…To investigate the proangiogenic mechanism of LPS-EXO, microRNA sequencing was performed to explore the microRNA profile of N-EXO and LPS-EXO. …”
Get full text

METTL16 controls airway inflammations in smoking-induced COPD via regulating glutamine metabolism by Xinyu Jia, Shan Liu, Chunan Sun, Manni Zhu, Qi Yuan, Min Wang, Tingting Xu, Zhengxia Wang, Zhongqi Chen, Mao Huang, Ningfei Ji, Mingshun Zhang

“…To identify the potential target genes and regulatory pathways through METTL16, methylated RNA immunoprecipitation sequencing (meRIP-seq), RNA sequencing (RNA-seq) and metabolomic profiling were used. …”
Get full text

Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC by Yulin Zhang, Yixu Wang, Jilin Peng, Kun Zhao, Ling Li, Yuan Zhang, Ziyu Zhai, Sijie Yuan, Shichao Li, Shichao Li, Shichao Li, Fanglei Ye, Le Wang

“…Knockdown of HNRNPC in the HNSCC cell lines HSC-3 and CAL-27 resulted in a significant decrease in proliferation, invasion, and malignant transformation abilities. RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation and sequencing (MeRIP-seq) data revealed that HNRNPC is involved in cell differentiation, cell migration and apoptosis. …”
Get full text

tiRNA-Gln-CTG is Involved in the Regulation of Trophoblast Cell Function in Pre-eclampsia and Serves as a Potent Biomarker by Yixiao Wang, Xiaohong Ji, Hengmei Shi, Sicong Liu, Hong Yu

“…Results: High-throughput sequencing of tsRNA was performed on plasma from pregnant women with PE and from healthy pregnant controls. …”
Get full text

Prevalence of β-lactam antibiotic resistance of Escherichia coli isolated from a neonatal intensive care unit by Jian Zhou, Jingqian Zhou, Min Chen, Pan Lü, Chunming Jiang

“…Genomic DNA was extracted for whole genome sequencing, construction of plasmid physical maps, locating resistance genes, and analyzing flanking regions and the resistance gene-related sequences. …”
Get full text

Genotype-Phenotype Correlation of G6PD Mutations among Central Thai Children with G6PD Deficiency by Boonchai Boonyawat, Tim Phetthong, Nithipun Suksumek, Chanchai Traivaree

“…Our study demonstrated the efficiency of direct DNA sequencing which can identify 12 missense mutations in Thai children.…”
Get full text

Improving genomic prediction accuracy of pig reproductive traits based on genotype imputation using preselected markers with different imputation platforms by J. Sun, J. Wei, Y. Pan, M. Cao, X. Li, J. Xiao, G. Yang, T. Yu

“…With the development of sequencing technology and price reduction, more and more genotype imputation panels of pig have been investigated, providing an effective and economical method to further study the genetic variation of pig economic traits. …”
Get full text

GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…Methods Human ovarian tissue specimens, RNA sequencing, GEPIA database and bioinformatics analyses were used to analyze the gene correlation, and to identify and validate potential downstream candidates. …”
Get full text

Characterization and molecular detection of pathogenicity and antibiotic resistance genes in Vibrio parahaemolyticus isolated from Pacific white shrimp by Muhammad Arif Mulya, Fachriyan Hasmi Pasaribu, Usamah Afiff, Munti Yuhana

“…The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). …”
Get full text

Knockdown of PDPN in astrocytes reduces hippocampal inflammation in T2DM mice by Xiaohang Wang, Xiaohang Wang, Xiaohang Wang, Qianqian Wang, Zhensheng Cai, Chengming Ni, Huan Wang, Hui Liu, Yusong Zhao, Jinbang Wang, Subo Liu, Xueying Wang

“…Subsequently GFAP-specific promoter adeno-associated virus(AAV)carrying interfering sequence was used to knockdown the key gene in astrocytes of T2DM mice. …”
Get full text

No rumen fermentation profiles and associated microbial diversities difference were found between Hu sheep and Karakul sheep fed a cottonseed hull diet by Jishu Wang, Guangxu E, Meng Zhang, Yanlong Zhang, Tiantian Bai, XuanXuan Pu, Junfeng Liu, Xuefeng Guo, Subir Sarker, Long Cheng

“…Results The 16S rRNA sequencing analysis revealed no significant differences in the relative abundance or dominant bacterial communities in the rumen across all stages. …”
Get full text