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Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation
Published 2020-07-01“…The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. …”
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A Theoretical Study On Factors Influencing Employees Performance, Rewards And Motivation Within Organisation
Published 2020-09-01“…The study of improving the efficiency of employees in the article is carried out in the following logical sequence: analyzed modern views on personnel management, studied the relationship between the structural elements of personnel management policy: efficiency, remuneration and motivation, developed a mechanism for managing staff efficiency. …”
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BLH3 Regulates the ABA Pathway and Lignin Synthesis Under Salt Stress in <i>Lilium pumilum</i>
Published 2025-06-01“…In this study, we investigated the molecular mechanism underlying the response of <i>LpBLH3</i> to salt stress in <i>Lilium pumilum</i> (<i>L. pumilum</i>) using various techniques, including quantitative PCR (RT-qPCR), determination of physiological indices of plant after Saline-Alkali stress, yeast two-hybrid screening, luciferase complementation imaging (LCI), and chromosome walking to obtain the promoter sequence, analyzed by PlantCARE, electrophoretic mobility shift assay (EMSA), and then dual-luciferase reporter assay(LUC). …”
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La reformulation et multimodalité dans un cours en visioconférence
Published 2015-11-01“…The three conversational sequences analyzed show how the tutors adapt their teaching regulations to the constraints of the device, which may result in the use of live chat and a more expressive body language to paraphrase what has been said.…”
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MOLECULAR CHARACTERIZATION OF MULTI-DRUG RESISTANCE ENTEROCOCCUS FAECALIS ISOLATED FROM MASTITIC COWS MILK
Published 2025-06-01“…E. faecalis gene was sequenced, analyzed, and registered in Gen-bank-NCBI and obtained the accession number (OP566380) that became a reference in Iraq and the world. …”
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Single-cell sequencing in diabetic retinopathy: progress and prospects
Published 2025-01-01“…Single-cell multi-omics sequencing integrates omics information, whereas stereo-sequencing analyzes gene expression and spatiotemporal data simultaneously. …”
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Transcriptome analysis of cadmium accumulation characteristics and fruit response to cadmium stress in Zunla 1 chili pepper
Published 2024-12-01“…Soil pot experiments involved collecting soil and chili pepper samples to measure Cd content. Transcriptome sequencing analyzed the chili pepper fruit’s transcriptome profile under two Cd levels: 0 μM (control) and 50 μM. …”
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Molecular diagnosis of begomovirus associated with leaf curl disease of Dahlia pinnata
Published 2018-12-01“…The fragments were cloned, sequenced, analyzed and submitted to GenBank under the accession numbers (MK122994-full-length) and (MK122995-betasatellites). …”
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First report of Leishmania RNA virus 2 and its high genetic diversity in cutaneous leishmaniasis cases from Ilam Province, Iran
Published 2025-08-01“…Phylogenetic analysis revealed high nucleotide sequence identity (96–99%) with LRV2 isolated from L. major strains from Iran, Turkey, and Uzbekistan. The four sequences analyzed revealed four distinct haplotypes, with high genetic variability among the isolates. …”
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Analisis Sekuensing Nucleotida pada Mutasi Gen MTHFR C677T pada Kondisi Premature Cardio Infarction
Published 2012-12-01“…Gen mutan MTHFR (C677T) diamplifikasi dan visualisasi melalui Automated Sequencer Analyzer dengan pembanding gen CBS exon 3, 7, dan 8 normal, serta data sekuens Gene Bank OMIM. …”
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mtDNA COI and cyt b Analysis of Carassius gibelio (Bloch, 1782) Living in Atatürk Dam Lake (Turkey)
Published 2022-09-01“…Although the same haplotype was detected for the mtDNA COI region with individuals living in Turkey before, a new haplotype was detected for the mtDNA cyt b gene region that was not previously defined. Conclusion: Sequence analyzes of mtDNA COI and cyt b gene regions of individuals belonging to the C. gibelio species living in Atatürk Dam Lake were performed for the first time in this study and compared with the data in the gene bank.…”
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Physicochemical Properties and Stability of Antioxidant Peptides from Swim Bladder of Grass Carp (<i>Ctenopharyngodon idella</i>)
Published 2025-03-01“…From 464 GCP-II sequences analyzed via LC-MS/MS, 7 bioactive peptides were selected based on antioxidant activity and functional sequence motifs, and were named peptides 1 to 7, respectively. …”
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Digital health projects financing: challenges and opportunities
Published 2021-03-01“…For gaining the research goal, this study was conducted in the following logical sequence: analyzing the effectiveness and stability of budget funding for the electronic health record projects in Ukraine within the eHealth initiative; defining the current state of Telemedicine and Mobile Medicine financings; identifying the potential directions for solving the problem of lack of funding based on world experience; determining the innovative digital medicine projects implemented by startups; setting the relationship between the amount of venture financing, the maturity and number of employees of companies. …”
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Investigating the renoprotective effects of Polygonatum sibiricum polysaccharides (PSP) on D-galactose-induced aging mice: insights from gut microbiota and metabolomics analyses
Published 2025-04-01“…Kidney pathology was assessed via H&E and Masson’s trichrome staining. 16S rRNA sequencing analyzed gut microbiota, and non-targeted metabolomics identified metabolic changes. …”
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Effect of <i>Elaeagnus angustifolia</i> Honey in the Protection Against Ethanol-Induced Chronic Gastric Injury via Counteracting Oxidative Stress, Interfering with Inflammation and...
Published 2025-05-01“…Western blot was employed to assess COX-2 protein expression, while 16<sup>S</sup> rRNA sequencing analyzed gut microbiota composition. The results demonstrated that EAH could play a role in gastric injury caused by long-term alcoholism by protecting gastric tissue structure, interfering with oxidative stress and inflammatory response, and remodeling the intestinal microbial community.…”
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Morphological, molecular and 3D synchrotron X-ray tomographic characterizations of Helicascus satunensis sp. nov., a novel mangrove fungus
Published 2024-11-01“…The morphology of this fungus was investigated and combined with multigene sequence analyzes of small subunit (SSU), large subunit (LSU), internal transcribed spacer (ITS) ribosomal DNA, translation elongation factor 1-alpha (TEF-1α) and RNA polymerase II (RPB2) genes. …”
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Spatiotemporal composition and diversity of endophyte communities in Dracaena cambodiana on Hainan Island
Published 2025-02-01“…Understanding the endophyte communities and their functional roles in resin production could enable the development of efficient induction techniques for resin production.MethodsIn this study, ITS and metagenomic sequencing analyzed endophyte communities’ characteristics and functional traits in different tissues and D. cambodiana across multiple wild populations on Hainan Island.ResultsWe identified distinct fungal genera that were dominant in different tissues. …”
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FOS as a biomarker for myocardial infarction treatment with Deng's Yangxin Decoction: a systems biology-based analysis
Published 2025-05-01“…Single-cell RNA sequencing analyzed hub biomarker expression in coronary plaques.ResultsFOS was a core biomarker for DYX for MI. …”
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Promoter polymorphisms in the JK*01 W.06 allele associated with the Jk(a + w) weak antigen phenotype
Published 2025-06-01“…Third-generation sequencing analyzed the JK gene haplotype sequences, quantifying the frequency of the JK*01 W.06 allele and characterizing promoter sequence variations. …”
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Comparative Analysis of Energy Efficiency in Conventional, Modular, and 3D-Printing Construction Using Building Information Modeling and Multi-Criteria Decision-Making
Published 2024-12-01“…Minitab is utilized for experimental design to determine the number and configurations of sequences analyzed. The Simple Additive Weighting (SAW) method, applied as an MCDM tool, is used to assess and rank the performance of sequences based on equally weighted criteria. …”
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