Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System by Ru Dai, Wei Hua, Wei Chen, Lidan Xiong, Li Li, Yiming Li

“…While these spheres were heterogeneous populations consisting of both proliferative and aggregated cells, the rate of proliferative cells correlated with a seeding density. tSKPs, isolated from FBs, were distinctive from FBs in cell cycle, marker expression, neural differentiation potential, and transcript profiles despite the two sharing partial similarity in certain properties. …”
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Functional and structural connectivity of the subregions of the amygdala in ADHD children with or without ODD by Zhao-Min Wu, Peng Wang, Xue-Chun Liu, Qing-Chao Zhou, Xiao-Lan Cao, Li Sun, Lu Liu, Qing-Jiu Cao, Li Yang, Yu-Feng Wang, Ying Qian, Bin-Rang Yang

“…Functional connectivity and structural connectivity were calculated using bilateral subregions of the Amygdala as seeds. Between-group voxel-wise comparisons were conducted. …”
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Biomass allocation between reproductive and vegetative organs of Artemisia along a large environmental gradient by Tumenjargal Tsogtsaikhan, Xuejun Yang, Ruiru Gao, Jiangrui Liu, Wenqiang Tang, Guofang Liu, Xuehua Ye, Zhenying Huang

“…Methods We collected 22 Artemisia species along a large environmental gradient in China and measured reproductive (infructescences including seeds) and vegetative (leaves, stems and roots) mass for each plant. …”
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Detection and decontamination of Mycoplasma in cultured cells and viral strains by Glaucia Prado de Andrade, Natália Hettwer Pedroso, Alice Becker, Vinicius Soares dos Santos, Juciane Bonella, José Valter Joaquim Silva Júnior, Rudi Weiblen, Eduardo Furtado Flores

“…After, a decontamination protocol of viral seeds was carried out, consisting of amplification in cell culture, centrifugation of the viral suspension, filtration, limiting dilution and culture in mycoplasma-free cells. …”
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Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels by Fransisca A. S. van Esterik, Behrouz Zandieh-Doulabi, Cornelis J. Kleverlaan, Jenneke Klein-Nulend

“…We aimed to test the osteogenic and/or vasculogenic differentiation potential as well as degradation of composites consisting of human adipose stem cells (ASCs) seeded on BCP60/40 or BCP20/80 incorporated in fibrin gels that trigger neovascularization for bone regeneration. …”
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Influence of sowing amount and substrate thickness on the quality of sandy ecological grass blankets by ZOU Jiatong, YU Weijie, ZHANG Zheng, FENG Zhidao, ZHANG Changlin

“…[Objective] Under the condition of ensuring the quality of the grass blanket, the study aims to select the appropriate combination of substrate thickness and seeding rate, and achieve the coordination and unity of ecological and economic effects in grass blanket production. …”
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Electrochemical properties of hydroxyapatite immobilization material for potential cytosensor fabrication by Dennis Adusei, Bernard O. Asimeng, Francis D. Krampa, Elvis K. Tiburu

“…The SHAp/PEDOT: PSS blend was used to modify a screen-printed carbon electrode (SPCE) by drop-casting, followed by seeding of pheochromocytoma (PC 12) and human embryonic kidney (HEK)-293T cells on the modified SPCE to record the EP using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). …”
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Effect of potassium rates and application methods in no-till on soil K availability and crop yield by Gustavo Pesini, Dayana Jéssica Eckert, Matheus Ferrari Menegat, Gustavo Frosi, João Pedro Moro Flores, Lucas Aquino Alves, Dionata Filippi, Tadeu Luis Tiecher, Danilo Rheinheimer dos Santos, Tales Tiecher

“…Each rate of K was applied in a band at the seeding row or broadcast on the soil surface. Grain yields and the available K content in the soil at different soil layers (0.00-0.05, 0.05-0.10, 0.10-0.15, and 0.15-0.20 m) were evaluated after the soybean harvest in 2020/2021. …”
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Successful establishment of CRISPR-based genome-edited clonal cell populations from primary cells of buffalo, goats, and sheep by Priyanka Singh, Bosco Jose, Devika Gautam, Aseem Tara, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…For promoting the growth of single cells, we used growth factor-supplemented media. After seeding a single cell to each well, the plate was kept undisturbed for 5-7 days, and then cell attachment rates were noted. …”
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Factors Affecting the Prognosis after Primary Tumor Resection for Patients with Metastatic Colorectal Cancer with Synchronous Peritoneal Metastasis: A Multi-center, Prospective, Ob... by Satoru Kagami, Kimihiko Funahashi, Hirotoshi Kobayashi, Kenjiro Kotake, Masayasu Kawasaki, Yusuke Kinugasa, Hideki Ueno, Kotaro Maeda, Takeshi Suto, Michio Itabashi, Heita Ozawa, Fumikazu Koyama, Shingo Noura, Hideyuki Ishida, Masayuki Ohue, Tomomichi Kiyomatsu, Soichiro Ishihara, Keiji Koda, Hideo Baba, Kenji Kawada, Yojiro Hashiguchi, Takanori Goi, Yuji Toiyama, Naohiro Tomita, Eiji Sunami, Fumihiko Fujita, Jun Watanabe, Kenichi Hakamada, Goro Nakayama, Kenichi Sugihara, Yoichi Ajioka

“…Methods: Patients were enrolled prospectively in the JSCCR project “Grading of Peritoneal Seeding in Colorectal Cancer.” Factors that may influence overall survival―age, sex, location of the primary tumor, lymph node metastasis, presence of liver metastasis, degree of peritoneal metastasis, peritoneal cancer index (PCI), cancer cure, and postoperative chemotherapy―in the PTR group were examined using multivariate analysis. …”
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Tiny clue reveals the general trend: a bibliometric and visualized analysis of renal microcirculation by Bing Wang, Mengting Xu, Sunjing Fu, Yingyu Wang, Hao Ling, Yuan Li, Bingwei Li, Xueting Liu, Qin Ouyang, Xiaoyan Zhang, Ailing Li, Xu Zhang, Mingming Liu

“…‘Cardiovascular outcome’ and ‘diabetic kidney diseases’ were identified as keywords in their burst period over the past three years.Conclusion Our bibliometric analysis illuminates the contours of nephrology and microcirculation research, revealing a landscape ripe for challenges and the seeds of future scientific innovation. While the trends discerned from the literature emerging opportunities in diagnostic innovation, renal microcirculation research, and precision medicine interventions, their translation to clinical practice is anticipated to be a deliberate process.…”
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Synergistic effects of PGPRs and fertilizer amendments on improving the yield and productivity of Canola (Brassica napus L.) by Haji Muhammad, Muhammad Ijaz, Abdul Sattar, Sami Ul-Allah, Ahmad Sher, Muhammad Asif, Muhammad Dilshad, Khalid Mahmood, Muhammad Waheed Riaz, Muhammad Saqlain Zaheer, Muhammad Rizwan, Salim Manoharadas

“…Conclusion In light of these results, we recommend the application of Bacillus subtilis to canola seeds along with either 2 t/ha biochar or the entire recommended dose of N: P:K (140:55:40 kg/ha). …”
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Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation by Guoyi Dong, Shengpeng Wang, Yuping Ge, Qiuting Deng, Qi Cao, Quanlei Wang, Zhouchun Shang, Wenjie OuYang, Jing Li, Chao Liu, Jie Tang, Weihua Zhao, Ying Gu

“…Here, we demonstrate that dissociated single MSCs seeded in induced pluripotent stem medium (MiPS) adhere loosely to the dish and spontaneously migrate to form spheroids during day 3 to day 6. …”
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Synergistic Effect of Sugarcane Bagasse and Zinc Oxide Nanoparticles on Eco-Remediation of Cadmium-Contaminated Saline Soils in Wheat Cultivation by Emad M. Hafez, Khadiga Alharbi, Hany S. Gharib, Alaa El-Dein Omara, Essam Elatafi, Maha M. Hamada, Emadelden Rashwan, Tarek Alshaal

“…ZnO NPs (25 mg L⁻¹) reduced Cd accumulation in wheat shoots by 43.3% and seeds by 46.3%, while SCB and ZnO NPs combined achieved a reduction of 74.1% and 62.9%, respectively. …”
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Consequences of changing regional integration on environmental development, agricultural markets, and food security by L.A. Omarbakiyev, S.M. Kantarbayeva, A.K. Nizamdinova, S.T. Zhumasheva, G.Zh. Seitkhamzina, A. Saulembekova

“…Internal changes will help the industry increase international competitiveness and efficiency while promoting sustainable development and ensuring food security and environmental safety.CONCLUSION: The article determined that it is necessary to prioritize developing multilateral partnerships to address transportation and logistics challenges in the export and import of agricultural products, and reduce dependence on the import of seeds, breeding products, fodder, and agricultural machinery. …”
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Optimization of Toxicity, Biodegradability, and Skin Irritation in Formulations Containing Mixtures of Anionic and Nonionic Surfactants Combined with Silica Nanoparticles by Manuela Lechuga, Mercedes Fernández-Serrano, Josefa Núñez-Olea, Juan Francisco Martínez-Gallegos, Francisco Ríos

“…Toxicity assessments were conducted using bacteria <i>Vibrio fischeri</i> for aquatic toxicity and <i>Lepidium sativum</i> seeds for terrestrial plant effects, revealing that individual surfactants like the anionic alkyl ether carboxylic acid EC-R_12–14E₃ exhibit high toxicity levels, while the nonionic fatty-alcohol ethoxylate FAE-R_12–14E₁₁ shows comparatively lower environmental impact. …”
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Effect of Sodium Azide on Quantitative and Qualitative Stem Traits in the M2 Generation of Ethiopian Sesame (Sesamum indicum L.) Genotypes by Micheale Yifter Weldemichael, Yemane Tsehaye Baryatsion, Desta Berhe Sbhatu, Girmay Gebresamuel Abraha, Hagos Mohammedseid Juhar, Abraha Birhan Kassa, Fiseha Baraki Sibhatu, Hailay Mehari Gebremedhn, Tesfakiros Semere Gebrelibanos, Mohammed Mebrahtu Mossa, Mullubrhan Mekonen Gebru, Birhanu Kahsay Meresa, Medhin Teklay, Birhanu Debesay Berhe, Haftay Abadi Gebru

“…The aim of this study was to examine the effect of NaN3 on quantitative and qualitative stem traits in the M2 generation of Ethiopian sesame (Sesamum indicum L.) genotypes. Seeds of fourteen sesame genotypes were used in this study and germinated and grown under greenhouse conditions. …”
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Production of organic fertilizer using pleurotus sp. as a process accelerator by A. Medina-Buelvas, A. Garcia-Guan, B. Barraza-Amador, E. Espinosa-Fuente, M. Del Castillo-Cabrales, K. Mendez-Gutiérrez, N. Rosales-Hernández

“…To evaluate the biological efficiency of the compost, two doses were tested with each 100 gram and 200 gram of fertilizer for each 500 gram of soil using corn seeds, which were sown in bags over a period of three months. …”
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Suppression of MCP-1, IFN-γ and IL-6 production of HNSCC ex vivo by pembrolizumab added to docetaxel and cisplatin (TP) exceeding those of TP alone is linked to improved survival by Jana Wellhausen, Louisa Röhl, Michael Berszin, Irene Krücken, Irene Krücken, Veit Zebralla, Veit Zebralla, Markus Pirlich, Markus Pirlich, Matthaeus Stoehr, Matthaeus Stoehr, Susanne Wiegand, Susanne Wiegand, Susanne Wiegand, Andreas Dietz, Andreas Dietz, Theresa Wald, Theresa Wald, Gunnar Wichmann, Gunnar Wichmann

“…As biomarkers for response to TP and pembrolizumab +TP are missing but may include cytokines, this work aims on determining cytokines potentially linked to outcome as prognostic markers sufficient to predict and/or monitor response to successful LOP.MethodsCollagenase IV digests were generated from 47 histopathological confirmed HNSCC tumor samples and seeded in 96-well plates containing pembrolizumab, docetaxel, cisplatin either solely or in binary or ternary combination. …”
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Impact of Arbuscular Mycorrhizal Symbiosis on Photosynthetic, Antioxidant Enzyme, and Water Flux Parameters in Salt-Stressed Chickpea (<i>Cicer arietinum</i>) Plants by Pooja Pooja, Sarita Devi, Sridevi Tallapragada, Yogesh K. Ahlawat, Nisha Sharma, Pankaj Kasnia, Nita Lakra, Rosa Porcel, Jose M. Mulet, Khalid M. Elhindi

“…<i>Rhizophagus fasciculatus</i> was used to inoculate the seeds of three different chickpea varieties (HC-3, CSG-8962, and C-235), and the physiological and biochemical changes of the AMF-inoculated and non-inoculated chickpea plants were studied. …”
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