Hypercalcemia in Small Lymphocytic Lymphoma with an Elevated Parathyroid Hormone-Related Peptide Associated with Early Richter Transformation by Ashish Bains, Pedro Covas, Olga Timofeeva, Michael Bromberg

“…We report a case of small lymphocytic lymphoma/chronic lymphocytic leukemia (SLL/CLL) associated with hypercalcemia and an elevation in parathyroid hormone-related peptide (PTHrP) in the setting of a Richter transformation. Real-time reverse transcriptase PCR on lymph node biopsy specimens obtained before and after transformation showed an 8-fold increase in PTHrP mRNA levels and about 2-fold decrease in the levels of its cognate receptor PTHR1. …”
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The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats by Yusu Ni, Diyan Chen, Yi Jiang, Danhong Qiu, Wen Li, Huawei Li

“…Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. …”
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Expression of Genes Associated with Efflux Pump and Porins in Acinetobacter baumannii Isolates Recovered from Different Clinical Specimens by Alya Amer Rahi, Huda Hadi Al-Hasnawy

“…Molecular detection of efflux pump genes (adeB and adeJ) was performed using polymerase chain reaction (PCR), and gene expression was measured by reverse transcriptase PCR. Results: A. baumannii isolates showed high resistance to antibiotics, especially β-lactams, carbapenems, and aminoglycosides, whereas colistin remained effective. …”
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Contribution of OqxAB Efflux Pump in Selection of Fluoroquinolone-Resistant Klebsiella pneumoniae by Orsolya Szabo, Bela Kocsis, Nikolett Szabo, Katalin Kristof, Dora Szabo

“…Quantitative reverse-transcriptase PCR was performed from each RNA sample. …”
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Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies by Sujin Kwon, Jung Sun Park, Byungkuk Min, Yong-Kook Kang

“…In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and fast-forming NGFP-iPSCs, have equivalent levels of pluripotency. Multiplex reverse-transcriptase PCR generated gene expression profiles (GEPs) of 13 pluripotency genes in single initially formed-iPSC (if-iPSC) colonies of NGFP and 4F2A group. …”
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Meta-analysis of COVID-19 prevalence during preoperative COVID-19 screening in asymptomatic patients by Menno R Vriens, Ellen de Bock, Mando D Filipe, Milan C Richir, Roger K J Simmermacher, A Christiaan Kroese

“…Therefore, this study aims to determine the prevalence of preoperative COVID-19, confirmed by reverse transcriptase PCR (RT-PCR), in asymptomatic patients.Design Systematic review and meta-analysis.Data sources Pubmed and Embase databases were searched through 20 February 2022.Eligibility criteria All COVID-19 articles including preoperative asymptomatic patients were included.Data extraction and synthesis Two independent reviewers extracted data and assessed risk of bias. …”
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Identification and Characterization of the Diverse Stress-Responsive R2R3-RMYB Transcription Factor from Hibiscus sabdariffa L. by Bahaeldeen Babikar Mohamed, Beenish Aftab, Muhammad Bilal Sarwar, Bushra Rashid, Zarnab Ahmad, Sameera Hassan, Tayyab Husnain

“…Differential display reverse transcriptase PCR and random amplification of cDNA ends (RACE) was used to explore the osmotic stress-responsive transcripts. …”
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ST Elevation Myocardial Infarction in Patients with COVID-19: Case Series by Sofia Lakhdar, Chandan Buttar, Laura Guzman Perez, Nso Nso, Mahmoud Nassar, Giovina Collura

“…The authors conducted a retrospective study of patients admitted to NYC Health + Hospitals, Queens, New York City, USA, with confirmed COVID-19 reverse transcriptase-PCR test findings between 29th March 2020 and 1st May 2020. …”
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Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy by Claudio Azzolini, Ilaria Stefania Pagani, Cristina Pirrone, Davide Borroni, Simone Donati, Muna Al Oum, Diana Pigni, Anna Maria Chiaravalli, Riccardo Vinciguerra, Francesca Simonelli, Giovanni Porta

“…Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. …”
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Deriving and validating a risk prediction model for long COVID-19: protocol for an observational cohort study using linked Scottish data by Jennifer K Quint, Aziz Sheikh, Chris Robertson, Srinivasa Vittal Katikireddi, Emily Moore, Colin R Simpson, Luke Daines, Eleftheria Vasileiou, Syed Ahmar Shah, Rachel H Mulholland, Vicky Hammersley, Steven Kerr, Ting Shi, David Weatherill, Elisa Pesenti

“…We will investigate the potential indicators of long-COVID by performing a matched analysis between those with a positive reverse transcriptase PCR (RT-PCR) test for SARS-CoV-2 infection and two control groups: (1) individuals with at least one negative RT-PCR test and never tested positive; (2) the general population (everyone who did not test positive) of Scotland. …”
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Molecular, Cytogenetic, and Hematological Analysis of Chronic Myeloid Leukemia Patients and Discovery of Two Novel Translocations by Muhammad Asif, Abrar Hussain, Abdul Wali, Nazeer Ahmed, Irfan Ali, Zafar Iqbal, Muhammad Amir, Muhammad Shafiq, Mahmood Rasool

“…Twenty-two samples of CML patients were evaluated by reverse transcriptase PCR and real-time PCR for the patients who failed to respond against imatinib mesylate. …”
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T lymphocytes induce human cancer cells derived from solid malignant tumors to secrete galectin-9 which facilitates immunosuppression in cooperation with other immune checkpoint pr... by Stephanie Schlichtner, Inna M Yasinska, Gurprit S Lall, Steffen M Berger, Sabrina Ruggiero, Dietmar Cholewa, Nijas Aliu, Bernhard F Gibbs, Elizaveta Fasler-Kan, Vadim V Sumbayev

“…Western blot analysis, ELISA, quantitative reverse transcriptase-PCR, on-cell Western and other measurement techniques were used to conduct the study. …”
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Contact patterns between index patients and their close contacts and assessing risk for COVID-19 transmission during different exposure time windows: a large retrospective observat... by Weibing Wang, Genming Zhao, Hao Pan, Bo Zheng, Chenyan Jiang, Yaxu Zheng, Huanyu Wu, Shenghua Mao, Ye Yao, Xiaohuan Gong, Sheng Lin, Bihong Jin, Dechuan Kong

“…Contacts exposed from 3 days before to 3 days after an asymptomatic index case had a positive reverse transcriptase-PCR (RT-PCR) result had a higher risk, with a maximum on day 0 (aRR=1.48; 95% CI 1.37, 1.59).Conclusions The greatest assortativity was for students and weakest for people working age not in the labour force. …”
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