Individualized gray matter morphological abnormalities unveil two neuroanatomical obsessive-compulsive disorder subtypes by Baohong Wen, Keke Fang, Qiuying Tao, Ya Tian, Lianjie Niu, Wenqing Shi, Zijun Liu, Jin Sun, Liang Liu, Xiaopan Zhang, Ruiping Zheng, Hui-Rong Guo, Yarui Wei, Yong Zhang, Jingliang Cheng, Shaoqiang Han

“…Sensitivity analyses were conducted to validate the reproducibility of clustering outcomes. To gain deeper insights into the connectomic and molecular underpinnings of structural brain abnormalities in the identified subtypes, we investigated their associations with normal brain network architecture and the distribution of neurotransmitter receptors/transporters. …”
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Current applications and challenges in large language models for patient care: a systematic review by Felix Busch, Lena Hoffmann, Christopher Rueger, Elon HC van Dijk, Rawen Kader, Esteban Ortiz-Prado, Marcus R. Makowski, Luca Saba, Martin Hadamitzky, Jakob Nikolas Kather, Daniel Truhn, Renato Cuocolo, Lisa C. Adams, Keno K. Bressem

“…Design limitations include 6 second-order and 12 third-order codes, such as lack of medical domain optimization, data transparency, and accessibility issues, while output limitations include 9 second-order and 32 third-order codes, for example, non-reproducibility, non-comprehensiveness, incorrectness, unsafety, and bias. …”
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Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid by Yusaku Noma, Hayate Sawayama, Kouta Yamashiro, Keitaro Nishi, Haruka Yoshinaga, Ryota Takaoka, Tsuyoshi Sugiura, Tomonori Sasahira, Tomofumi Hamada, Yasuo Suda, Tatsuo Okui

“…We also applied the assay to a test set of gargle fluid from 10 patients with oral cancer and 10 control subjects, and the results demonstrated the reproducibility of the diagnostic performance. In light of these findings, we propose that this new method could become a noninvasive and convenient screening tool for the detection of oral cancer.…”
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Comparative analysis of deep learning and radiomic signatures for overall survival prediction in recurrent high-grade glioma treated with immunotherapy by Qi Wan, Clifford Lindsay, Chenxi Zhang, Jisoo Kim, Xin Chen, Jing Li, Raymond Y. Huang, David A. Reardon, Geoffrey S. Young, Lei Qin

“…Manual segmentation of the tumor components is time-consuming and poses significant reproducibility issues. We compare the prediction of overall survival (OS) in recurrent high-grade glioma(HGG) patients undergoing immunotherapy, using deep learning (DL) classification networks along with radiomic signatures derived from manual and convolutional neural networks (CNN) automated segmentation. …”
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Linking Research Data with Physically Preserved Research Materials in Chemistry by Chia-Lin Lin, Pei-Chi Huang, Simone Gräßle, Christoph Grathwol, Pierre Tremouilhac, Sylvia Vanderheiden, Patrick Hodapp, Sonja Herres-Pawlis, Alexander Hoffmann, Fabian Fink, Georg Manolikakes, Till Opatz, Andreas Link, M. Manuel B. Marques, Lena J. Daumann, Manuel Tsotsalas, Frank Biedermann, Hatice Mutlu, Eric Täuscher, Felix Bach, Tim Drees, Steffen Neumann, Shashank S. Harivyasi, Nicole Jung, Stefan Bräse

“…A big step towards transparency and reproducibility of the scientific work can be gained if scientists publish their data in research data repositories in a FAIR manner. …”
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Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening by Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav, Omkar Suhas Vinchure, Nazlican Altinisik, Hannes Windoffer, Sarah Muller, Ina Rothenaigner, Sean Lin, Aruljothi Mariappan, Dhanasekaran Rathinam, Ali Mirsaidi, Olivier Goureau, Lucia Ricci-Vitiani, Quintino Giorgio D’Alessandris, Bernd Wollnik, Alysson Muotri, Limor Freifeld, Nathalie Jurisch-Yaksi, Roberto Pallini, Christine R. Rose, Volker Busskamp, Elke Gabriel, Kamyar Hadian, Jay Gopalakrishnan

“…Significant hindrances, however, are morphological and cellular heterogeneity, inter-organoid size differences, cellular stress, and poor reproducibility. Here, we describe a method that reproducibly generates thousands of organoids across multiple hiPSC lines. …”
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Development and cognitive testing of a food-frequency questionnaire to assess intake of plant-based protein foods among older adults by Virginie Drolet-Labelle, Alexandra Bédard, Simone Lemieux, Vicky Drapeau, Lana Vanderlee, Danielle Laurin, Sophie Desroches

“…Discussion: Our FFQ provides a comprehensive measure of PBP intake, is well understood by older adults in Québec and will support rigorous assessment of PBP intake in this population but requires further validation to confirm its validity and reproducibility. …”
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Optical time-lapsed in situ mechanochemical studies on metal halide perovskite systems by Yonghao Xiao, David G. Bradley, Wei Xin Chan, Xinwen Hu, Lian Xiao, Zhenpeng Liu, Zi Yang Tan, Xin Zhao, Chenfei Li, Mansour Sadek, Vikash Kumar Ravi, Ying Hern Tam, Xiaotao Wang, Felipe García, Handong Sun, Seow Chin Low, Poh Boon Phua, Tze Chien Sum, Xiuwen Zhou, Yee-Fun Lim, John V. Hanna, Han Sen Soo

“…Abstract Metal halide perovskites are attractive for optoelectronic applications, but the existing solution-based synthetic methods rely on hazardous solvents and lack reproducibility. To overcome these challenges, solvent-free mechanochemical synthesis of perovskites can be conducted, although traditional opaque ball milling equipment hinder real-time monitoring of the reaction progress. …”
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Refined methodology for quantifying Pseudomonas aeruginosa virulence using Galleria mellonella by Christopher M. R. Axline, Travis J. Kochan, Sophie Nozick, Timothy Ward, Tania Afzal, Issay Niki, Sumitra D. Mitra, Ethan VanGosen, Julia Nelson, Aliki Valdes, David Hynes, William Cheng, Joanne Lee, Prarthana Prashanth, Timothy L. Turner, Nathan B. Pincus, Marc H. Scheetz, Kelly E. R. Bachta, Alan R. Hauser

“…We also describe a quality control metric for enhancing the reproducibility of LT50 values. This approach provides an accurate and reproducible methodology for using G. mellonella larvae to measure and compare the virulence of P. aeruginosa strains.IMPORTANCEPseudomonas aeruginosa is a significant cause of morbidity and mortality. …”
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Standardization, validation, and comparative evaluation of a convenient surrogate recombinant vesicular stomatitis virus plaque reduction test for quantification of Hantaan orthoha... by Jing Wei, Hui Zhang, Jiawei Pei, Qiqi Yang, Yuan Wang, Xiaolei Jin, He Liu, Liang Zhang, Hongwei Ma, Linfeng Cheng, Yangchao Dong, Yingfeng Lei, Yinlan Bai, Zhikai Xu, Pengbo Yu, Fanglin Zhang, Wei Ye

“…We observed positive correlations between two neutralization assays among HFRS patients and inactive vaccine recipients (R2 = 0.5994 and 0.3440, respectively) and confirmed the clear specificity with healthy donors without vaccinated and reproducibility with three more assays. Our results suggest that rVSV-HTNV-GP based PRNT is a reliable lower-biosafety level surrogate for HTNV NAbs evaluation, which is easy to perform with higher sensitivity. …”
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Specific detection of pigeon parvovirus with TaqMan real-time PCR technology by Cuiteng Chen, Chunhua Zhu, Shuyu Chen, Zhen Chen, Huanru Fu, YuYi Chen, Mengyan Zhang, Wenyu Zhang, Yu Huang, Longfei Cheng, Chunhe Wan

“…In conclusion, a TaqMan-based fluorescence quantitative PCR method was developed, with the advantages of sensitivity, specificity, and reproducibility. This method can be used for further epidemiological monitoring of PiPV infection.…”
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Refining Established Practices for Research Question Definition to Foster Interdisciplinary Research Skills in a Digital Age: Consensus Study With Nominal Group Technique by Jana Sedlakova, Mina Stanikić, Felix Gille, Jürgen Bernard, Andrea B Horn, Markus Wolf, Christina Haag, Joel Floris, Gabriela Morgenshtern, Gerold Schneider, Aleksandra Zumbrunn Wojczyńska, Corine Mouton Dorey, Dominik Alois Ettlin, Daniel Gero, Thomas Friemel, Ziyuan Lu, Kimon Papadopoulos, Sonja Schläpfer, Ning Wang, Viktor von Wyl

“…We propose 3 clusters of contextual and methodological adaptations to this workflow to better integrate data science practices: (1) acknowledging real-life constraints and limitations in research scope; (2) allowing more iterative, data-driven approaches to research question formulation; and (3) strengthening research quality through reproducibility principles and adherence to the findable, accessible, interoperable, and reusable (FAIR) data principles. …”
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Reliability of radiomics features as imaging biomarkers for evaluating brain aging: A study based on myelin protein and diffusion tensor imaging by Yuting Yan, Mengmeng Hu, Xiaodong He, Yuyun Xu, Xiaojun Sun, Jiaxuan Peng, Fanfan Zhao, Yuan Shao

“…Ten middle-aged and ten old-aged mice were assigned to the testing set to further validate the reproducibility of the features. T2-weighted imaging and DTI were conducted to obtain white matter radiomics features and DTI parameters. …”
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Impact of Cordyceps sinensis on coronary computed tomography angiography image quality and renal function in a beagle model of renal impairment by Peiji Song, Peiji Song, Kun Li, Xiaodie Xu, Guifeng Zhang, Zengkun Wang, Linbing Sun, Zekai Zhao, Ting Li, Ximing Wang, Zhangyong Xia, Zhangyong Xia, Zhangyong Xia, Zhangyong Xia

“…A significant correlation was found between MRI parameters and laboratory renal function markers, with excellent inter- and intra-observer reproducibility, and high repeatability in the measurements.ConclusionHR-dependent personalized CM CCTA imaging did not compromise image quality. …”
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Assessment of Lower Limb Muscle Volume Using 3D Ultrasonography: Validity and Reliability Compared to MRI by Paul Ritsche, Romina Ledergerber, Michele Pansini, Francesco Santini, Oliver Faude

“…Our openly accessible documentation of the methodology supports reproducibility and further innovation in the field. …”
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Validación y adaptación cultural al español de la Rúbrica de retroalimentación del desempeño quirúrgico (SPR) by Jaime Andrés Leal, Daniela Gutiérrez Zúñiga, Stephanie Renza, Felipe Valbuena

“…The psychometric validation assessed face, content, convergent validity, internal consistency, and reproducibility. Results: A total of 160 evaluations using the SPR were conducted, of which 129 were suitable for analysis. …”
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Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy by Yao WANG, Yingying SUN, Yubing JING, Xiyan YANG, Yanfei LI, Jiaxiang HUANG, Xiujin CHEN, Zhaozhou LI, Li CAO, Huaibin KANG

“…Results showed that AuNPs with a particle size of 40 nm had good enhancement effect, the SERS characteristic peak of LAS was at 1118 cm−1, the AuNPs substrate had good reproducibility, and the optimal addition concentration of HNO3 was 0.5 mol/L. …”
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Development and validation of a food-related quality of life questionnaire (CANUT-QVA) for cancer patients by Kenza Drareni, Catherine Mercier, Reisya Rizki Riantiningtyas, Anestis Dougkas, Benjamin Riche, Pascale Roux, Chantal Fingal, Hélène Labrosse, Fadila Farsi, David Dayde, Mélanie Roche, Julie Anne Nazare, Arnaud Fournel, Delphine Maucort-Boulch, Moustafa Bensafi, Véronique Mourier, Amandine Bruyas, Agnès Giboreau

“…Analysis of test-retest reliability was also performed to evaluate reproducibility. Results: Out of the 46 items, 39 items showed significant differences between patients and healthy controls. …”
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超高效液相色谱-三重四极杆串联质谱法同时 测定植物油中4种维生素E异构体Simultaneous determination of four vitamin E isomers in vegetable oil by ultra-high performance liquid chromatography- triple quadrupole... by 李彩玲1，张见1,2，刘元法1,3，王晓超1，梅东旭1 LI Cailing1, ZHANG Jian1,2, LIU Yuanfa1,3, WANG Xiaochao1, MEI Dongxu1

“…This method has the advantages of accuracy, sensitivity, and good reproducibility, and it can be applied to the analysis of actual samples. …”
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Pragmatic algorithm for visual assessment of 4-Repeat tauopathies in [18F]PI-2620 PET Scans by Theresa Bauer, Matthias Brendel, Mirlind Zaganjori, Alexander M. Bernhardt, Alexander Jäck, Sophia Stöcklein, Maximilian Scheifele, Johannes Levin, Thilo van Eimeren, Alexander Drzezga, Osama Sabri, Henryk Barthel, Robert Perneczky, Günter Höglinger, Nicolai Franzmeier, Johannes Gnörich

“…This study aims to investigate the effectiveness of visual evaluation of [18F]PI-2620 images for diagnosing 4R-tauopathies and to develop a straight-forward reading algorithm to improve objectivity and data reproducibility. Methods: A total of 83 individuals with [18F]PI-2620 PET scans were included. …”
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