Construction of oncolytic reporter influenza viral vectors and assessment of their safety following intracranial administration in rats by A. A. Pulkina, A. S. Mustafaeva, E. A. Romanovskaya-Romanko, M. A. Plotnikova, O. O. Ozhereleva, M. A. Shuklina, L. N. Kiseleva, Y. S. Alekseeva, N. S. Kurmazov, A. R. Muslimov, A.-P.S. Shurygina, M. A. Stukova

“…METHODS: Chemiluminescent reporter influenza A virus strains were obtained by reverse genetics. …”
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Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) by Aistė Petruškevičiūtė, Ugnė Šimuliūnaitė, Catalina M. Polanco, Barbara Rojas, Simonas Kuras, Beatričė Valatkaitė-Rakštienė, Rimvydas Norvilas, Akshay Kumar Vijaya, Patricia Muñoz, Urtė Neniškytė, Artūras Jakubauskas, Aurelijus Burokas, Ivan Nalvarte, Jose Inzunza, Daniel Naumovas, Mindaugas Stoškus, Laimonas Griškevičius, Daiva Baltriukienė, Jonathan Arias

Subjects: “…Genetically encoded reporter…”
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HSP70-Inducible hNIS-IRES-eGFP Reporter Imaging: Response to Heat Shock by Jiantu Che, Mikhail Doubrovin, Inna Serganova, Lyudmila Ageyeva, Tatiana Beresten, Ronald Finn, Ronald Blasberg

“…The pQHNIG70 reporter system can be used to study stress and drug responses in transduced cells and tissues.…”
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Comparison of and as Reporter Genes for Noninvasive Imaging of Bone Mesenchymal Stem Cells Transplanted into Infarcted Rat Myocardium by Shuo Hu, Wei Cao, Xiaoli Lan, Yong He, Juntao Lang, Congjiao Li, Jia Hu, Rei An, Zairong Gao, Yongxue Zhang

“…Both rNIS and hNIS are useful reporter genes to monitor BMSCs transplanted into infarcted myocardium in vivo with rNIS being superior to hNIS as the reporter gene.…”
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Optimizing Imaging of Three-Dimensional Multicellular Tumor Spheroids with Fluorescent Reporter Proteins Using Confocal Microscopy by Lucia le Roux, Andrei Volgin, David Maxwell, Katashi Ishihara, Juri Gelovani, Dawid Schellingerhout

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Reversible Electroporation–Mediated Liposomal Doxorubicin Delivery to Tumors Can Be Monitored With Zr-Labeled Reporter Nanoparticles by Govindarajan Srimathveeravalli PhD, Dalya Abdel-Atti BS, Carlos Pérez-Medina PhD, Haruyuki Takaki MD, PhD, Stephen B. Solomon MD, Willem J. M. Mulder PhD, Thomas Reiner PhD

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In silico identification of switching nodes in metabolic networks by Mairet, Francis

Subjects: “…Genome-scale metabolic model, Branching point, Reporter metabolites, Flux Balance Analysis…”
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Reports by D. Jean Lodge, Sharon A. Cantrell, Jennifer Luangsa-ard, Ali Ammarellou, Ahmed Abdel-Azeem, Ramon Alberto Batista Garcia, Sanjay K. Singh

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Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter by Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen

“…We describe steps for designing guide RNA to direct Cas9 endonuclease to consensus splice sites, producing transgenic mice through pronuclear injection, and screening for desired mutations in cultured mammalian cells using a minigene splicing reporter. Splice isoform-specific mouse mutants provide valuable tools for genetic analyses beyond loss-of-function and transgenic alleles.For complete details on the use and execution of this protocol, please refer to Dailey-Krempel et al.1 and Johnson et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Neuron-Specific Fluorescence Reporter-Based Live Cell Tracing for Transdifferentiation of Mesenchymal Stem Cells into Neurons by Chemical Compound by Do Won Hwang, Hyun Woo Kwon, Jaeho Jang, Hee Jung Jung, Kwang Rok Kim, Dong Soo Lee

“…Plasmid vector containing red fluorescence reporter genes under the control of the tubulin α1 (Tα1) promoter (pTα1-DsRed2) traced the neuronal differentiation of MSCs. …”
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Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells by Vanessa R. Bevilaqua, Gabriel F. Pelentir, Moema A. Hausen, Eliana A. R. Duek, Vadim R. Viviani

“…Firefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes. …”
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