Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) by Wenyue Huang, Shifan Zhang, Fei Li, Hui Zhang, Rifei Sun, Guoliang Li, Shujiang Zhang

“…Analysis of the promoter cis-acting elements of the <i>BraTSD2</i> gene revealed that the promoter is regulated by signals associated with light response, abscisic acid, stress, salicylic acid, and MYBHI. …”
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Role and prognostic value of growth differentiation factor 15 in patient of heart failure with preserved ejection fraction: insights from the PURSUIT-HFpEF registry by Tetsuhisa Kitamura, Shungo Hikoso, Takahisa Yamada, Yoshio Yasumura, Akito Nakagawa, Toshihiro Takeda, Hirota Kida, Akihiro Sunaga, Tomoharu Dohi, Katsuki Okada, Daisaku Nakatani, Yasushi Matsumura, Yasushi Sakata, Shunsuke Tamaki, Takaharu Hayashi, Yoshiharu Higuchi, Masaharu Masuda, Mitsutoshi Asai, Toshiaki Mano, Hisakazu Fuji, Daisaku Masuda, Shizuya Yamashita, Masami Sairyo, Yusuke Nakagawa, Haruhiko Abe, Yasunori Ueda, Kunihiko Nagai, Masamichi Yano, Masami Nishino, Jun Tanouchi, Yoh Arita, Shinji Hasegawa, Takamaru Ishizu, Minoru Ichikawa, Yuzuru Takano, Eisai Rin, Tetsuya Watanabe, Shiro Hoshida, Masahiro Izumi, Hiroyoshi Yamamoto, Hiroyasu Kato, Kazuhiro Nakatani, Mayu Nishio, Keiji Hirooka, Takahiro Yoshimura, Akihiro Tani, Yasushi Okumoto, Yasunaka Makino, Katsuomi Iwakura, Yoshiyuki Kijima, Takashi Kitao, Masashi Fujita, Koichiro Harada, Masahiro Kumada, Osamu Nakagawa, Ryo Araki, Takayuki Yamada, Fusako Sera, Kei Nakamoto, Hidetaka Kioka, Tomohito Ohtani, Nobuyuki Ogasawara, Yukinori Shinoda, Koichi Tachibana, Yohei Sotomi, Taiki Sato, Yuji Yasuga, Toshinari Onishi, Kazunori Kashiwase, Ryu Shutta, Masahiro Seo, Yuki Matsuoka, Yasuhiro Akazawa, Daisuke Sakamoto

“…Background Growth differentiation factor 15 (GDF15) is a cytokine responding to oxidative stress and inflammation, and it regulates appetite and energy balance. The association between GDF15 and clinical factors and its prognostic value in elderly multimorbid patients with heart failure with preserved ejection fraction (HFpEF) have not been well unknown.Methods This exploratory analysis is part of the Prospective mUlticenteR obServational stUdy of patIenTs with Heart Failure with preserved Ejection Fraction study (N=1231), an ongoing, prospective, multicentre observational study of acute decompensated HFpEF (UMIN000021831). …”
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Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement by Fumitoshi Ohori, Hideki Kitaura, Aseel Marahleh, Akiko Kishikawa, Saika Ogawa, Jiawei Qi, Wei-Ren Shen, Takahiro Noguchi, Yasuhiko Nara, Itaru Mizoguchi

“…Osteocytes express receptor activator of nuclear factor kappa-B ligand (RANKL) and regulate osteoclast formation. Orthodontic tooth movement (OTM) occurs by osteoclast resorption of alveolar bone. …”
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Optimization of fermentation conditions to increase the production of antifungal metabolites from Streptomyces sp. KN37 by Xiaoyue Yang, Lijing Yuan, Muhammad Zeeshan, Chuntian Yang, Wen Gao, Guoqiang Zhang, Chunjuan Wang

“…Transcriptomic analysis further elucidated molecular mechanisms behind these changes with the expression of salicylic acid dehydrogenase (SALD) was significantly down-regulated, which was only 0.48 times compared to that before optimization. …”
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Targeting CXCL8 signaling sensitizes HNSCC to anlotinib by reducing tumor-associated macrophage-derived CLU by Xin Hu, Yikang Ji, Mi Zhang, Zhihui Li, Xinhua Pan, Zhen Zhang, Xu Wang

“…Understanding the spatial heterogeneity of glucose deficiency can reveal the molecular mechanisms regulating cancer metabolism and identify therapeutic targets to improve effective nutrient-starvation therapies. …”
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Bioelectric stimulation outperforms brain derived neurotrophic factor in promoting neuronal maturation by María del Pilar Diego-Santiago, María Ujué González, Esther María Zamora Sánchez, Nuria Cortes-Carrillo, Carlos Dotti, Francesc Xavier Guix, Sahba Mobini

“…Additionally, we address the question regarding which ES parameters regulate biological responses. The neuronal differentiation and maturation of SH-SY5Y cells were tested under several pulsed ES regimes. …”
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Promotion of microfracture-mediated cartilage repair by the intra-articular injection of Mg2+ by Zhian Chen, Tianhua Zhou, Zhengbo Yin, Peiya Duan, Ying Zhang, Yujiao Feng, Rongmao Shi, Yongqing Xu, Rongqing Pang, Hongbo Tan

“…The RT-qPCR results showed that sirtuin 1/bone morphogenetic protein-2/sex-determining region Y box 9 (SIRT1/BMP-2/SOX-9) and hypoxia-inducible factor 1-alpha (HIF-1α) messenger RNA levels were up-regulated after Mg2+ injection. Conclusion: MFX combined with Mg2+ treatment has a positive effect on cartilage repair. …”
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Effects of Nicotinamide Riboside Supplementation on Postmortem Mitochondrial Functionality and Apoptotic Activation by Chen Zhu, Luke A. Whitcomb, Adam J. Chicco, Morgan E. Gravely, Hanna M. Alcocer, Daniela A. Alambarrio, John M. Gonzalez, Colton L. Smith, Mahesh N. Nair, Huey Yi Loh, Terry E. Engle, Arya Niraula, Chaoyu Zhai

“…Longissimus dorsi muscle samples were collected at 45 min and 24 h postmortem and analyzed for mitochondrial functionality using high-resolution respirometry and apoptotic protein abundance (apoptosis regulator Bcl-2-associated X (BAX), apoptotic inducing factor (AIF), and caspase 3 (CASP3)) via immunoblotting. …”
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Efek Pemberian Suntikan Subkutan Vitamin C Terhadap Luka Insisi Dermal by Surya Darma, Menker Manjas, Deddy Saputra, Salmiah Agus, Erkadius Erkadius

“…Besides that, vitamin C also functions in regulating and stabilizing procollagen mRNA gen transcription in dermal collagen synthesis. …”
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Establishment of the fingerprint of ganoderma lucidum spore powder and study on the anti-inflammatory activity of ganoderma lucidum spore oil by Yu Zhang, Lihui Hou, Le Wang, Yunshan Si, Hongjuan Wen, Ying Gao, Xiuhua Yu

“…[Results] The HPLC fingerprints were calibrated with a total of 12 peaks, of which peaks 1-5 were ganoderic acid C2, ganoderic acid G, ganoderic acid B, ganoderic acid A, ganoderic acid D, respectively; the current version of the Pharmacopoeia method of HPTLC showed that 10 batches of samples complied with the regulations, and the quality of raw materials of different origins was found to be similar to that of the HPLC fingerprints after 2 unfoldings by using trichloromethane-acetonitrile-methanol-formic acid (26∶4 ∶1∶1) as the unfolding agent. …”
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ANGPTL4: A Predictive Marker for Diabetic Nephropathy by Eman Al Shawaf, Mohamed Abu-Farha, Sriraman Devarajan, Zahra Alsairafi, Irina Al-Khairi, Preethi Cherian, Hamad Ali, Aditi Mathur, Fahd Al-Mulla, Abdulnabi Al Attar, Jehad Abubaker

“…ANGPTL4 is a glycoprotein that is involved in regulating triglyceride metabolism by inhibiting LPL activity under fasting conditions. …”
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Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper by Alexandra Rucavado, Teresa Escalante, Catarina F. P. Teixeira, Cristina María Fernándes, Cecilia Díaz, José María Gutiérrez

“…This suggests that MMP activity was regulated by a highly controlled activation of latent forms and, probably, by a concomitant synthesis of MMP inhibitors. …”
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Circ_0001084/miR-181c-5p/PTPN4 Axis Mitigates Cardiomyocyte Injury by Modulating the TLR4/NF-&kappa;B Pathway: Insights into Therapeutic Potential for Myocardial Reperfusion Injury by Deng H, Fan Q, Huang L, Ouyang W, Zhu W

“…Non-coding RNAs, including miRNAs, have been increasingly recognized for their roles in regulating cardiomyocyte responses to hypoxia/reoxygenation (H/R) injury. miR-181c-5p, in particular, has been implicated in inflammatory and apoptotic processes, suggesting its potential involvement in exacerbating cellular damage.Methods: This study combined bioinformatic and experimental techniques to investigate myocardial injury. …”
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In-Situ Electrospinning Dressings Loaded with Kaempferol for Reducing MMP9 to Promote Diabetic Ulcer Healing by Li J, Meng H, Guo W, Zhou L, Wu S, Gao G, Liu Q, You D, Qu W

“…Histological and immunofluorescence staining were performed to determine the status of epithelization, collagen deposition, MMP9 expression, macrophage polarization, inflammation response and angiogenesis.Results: Material science experiments have shown that the dressing has optimal fiber micromorphology and good water vapor transport properties (WVTR: 4.88 kg m− 2 24h− 1); in vivo, diabetic wound experiments have shown that the high porosity and pharmacological effects of PED4 can mutually promote the rapid healing of diabetic wounds (healed 95.9% on day 15), facilitate the transformation of macrophages from M1-type to M2-type and regulate the expression of MMP9.Conclusion: Portable electrospinning dressings equipped with kaempferol not only better fit irregular wounds, but also promote wound healing through MMP9 and macrophage polarization. …”
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Midgut immune profiling and functional characterization of Aedes aegypti ABC transporter gene(s) using systemic and local bacterial challenges by Vikas Kumar, Shilpi Garg, Diksha Sisodia, Lalita Gupta, Sanjeev Kumar, Vishal Saxena

“…In contrast, midgut bacterial load was significantly regulated by induction of defensin A and cecropin G in the late hours of local bacterial challenges in AaeABCG3-silenced mosquitoes. …”
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Impact of Prenatal Stress on Neuroendocrine Programming by Odile Viltart, Christel C. A. Vanbesien-Mailliot

“…In this view, evolution has selected tightly regulated processes aimed at maintaining stability among internal parameters despite external changes, a process termed homeostasis. …”
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The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis by Uun Yanuhar, Achmad Khumaidi

“…An outcome from gene transcription, the protein protects against pathogen infection, such as receptor clustering, antigen internalization, and regulating vesicle for antigen processing. ß - actin expression will determine the success of immune response of the organism. …”
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Collaborative design of a care pathway for pharmacy-based PrEP delivery in Nigeria: insights from stakeholder consultation by Obinna I. Ekwunife, Theodora C. Omenoba, Ugochi Eyong, Valentine Okelu, Michael Alagbile, Ifeanyi Ume, Ambrose Eze, Aderinola Fisayo, Gloria Aidoo-Frimpong, Farah Shroff, Chimezie Anyakora

“…The participants were purposefully selected to ensure diverse representations, including youth who are potential PrEP users, pharmacy providers, regulators, and representatives from civil society organizations. …”
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Large language models generating synthetic clinical datasets: a feasibility and comparative analysis with real-world perioperative data by Austin A. Barr, Joshua Quan, Eddie Guo, Emre Sezgin, Emre Sezgin

“…BackgroundClinical data is instrumental to medical research, machine learning (ML) model development, and advancing surgical care, but access is often constrained by privacy regulations and missing data. Synthetic data offers a promising solution to preserve privacy while enabling broader data access. …”
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Activated Growth Factor From Platelets as Treatment for Diabetic Retinopathy Through Antioxidant-Oxidative Stress Pathway by Amin R, Hidayat R, Maritska Z, Putri TW

“…This study was aimed to evaluate activated growth factor (AGF) from platelet for diabetic retinopathy treatment, utilizing an in vivo investigation to regulate the antioxidant-oxidative stress pathway.Methods: The activated growth factor was initially derived by extracting intravenous blood from the rats. …”
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