A Full GMP Process to Select and Amplify Epitope-Specific T Lymphocytes for Adoptive Immunotherapy of Metastatic Melanoma by N. Labarriere, A. Fortun, A. Bellec, A. Khammari, B. Dreno, S. Saïagh, F. Lang

“…In the present work, we provide a detailed description of the whole GMP process of sorting and amplification of clinical grade T cells specific for the melanoma antigens Melan-A and MELOE-1. All the reagents used in this process including the sorting reagent were produced in GMP conditions and we document the optimization of the different steps of the process such as peptide stimulation, sorting, and amplification. …”
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Influence of Conventional and Ultrasonic-Assisted Extraction on Phenolic Contents, Betacyanin Contents, and Antioxidant Capacity of Red Dragon Fruit (Hylocereus polyrhizus) by Nurul Shazini Ramli, Patimah Ismail, Asmah Rahmat

“…Total phenolic content (TPC) was determined using Folin-Ciocalteu reagent while quantitative determination of total flavonoid content (TFC) was conducted using aluminium trichloride colorimetric method. …”
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Flowsheets for the Validation of the Reference AmSEL System by Vanel Vincent, Wilden Andreas, Modolo Giuseppe, Geist Andreas, Montuir Marc

“…The objective of this work is to design a flowsheet for the Am stripping and Cm re-extraction steps to recover americium selectively from Cm and Ln, with TODGA as extractant and SO3-Ph-BTBP as complexing reagent. The test was implemented in August 2023 at FZ Jülich with trace amounts of americium and curium.…”
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Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator by Esin Akgul Kalkan, Mehtap Sahiner, Dilek Ulker Cakir, Duygu Alpaslan, Selehattin Yilmaz

“…Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent, an analytical method was developed for the quantitative determination of acetone in human blood. …”
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Design and Optimization of Colorimetric Paper-Based Analytical Device for Rapid Detection of Allopurinol in Herbal Medicine by Rimadani Pratiwi, Rieda Nurwulan Septyani, Rossi Febriany, Febrina Amelia Saputri, Rina Fajri Nuwarda

“…There were 5 specific reagents that reacted positively with allopurinol and only 3 reagents that can be applied to the paper, that is, Folin-Ciocalteu, Tollens, and p-DAB reagent. …”
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Radical Scavenging, Proteases Activities, and Phenolics Composition of Bark Extracts from 21 Medicinal Plants by Muhammad Asam Raza, Durre Shahwar, Tania Khan

“…Total phenolic contents, determined using FC (Folin Ciocalteu) reagent, ranged from 528 to 715 mg GAE/g of crude extract. 15 out of 21 plants showed DPPH activity more than 90% and the rest of plants exhibited the activity in the range of 87–89%. …”
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Isolation of Escherichia coli 0157:H7 Strain from Fecal Samples of Zoo Animal by Aseel Mohammed Hamzah, Aseel Mohammed Hussein, Jenan Mahmoud Khalef

“…E. coli O157:H7 isolates were distinguished by agglutination with E. coli O157:H7 latex reagent (Oxoid), identified among the isolates, which showed that multiple E. coli strains were isolated from one petting zoo animal, in which a single animal simultaneously shed multiple E. coli strains; E. coli O157:H7 was isolated only by selective enrichment culture of 2 g of petting zoo animal feces. …”
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(Pyridin-2-ylmethyl)porphyrins: synthesis, characterization and C–N oxidative fusion attempts by Berthelot, Mathieu, Echaubard, Julie, Bousfiha, Asmae, Devillers, Charles H.

“…A new route for the synthesis of meso-(pyridin-2-ylmethyl)porphyrins was developed, based on the nucleophilic attack of (pyridin-2-ylmethyl)lithium reagent on a porphyrin bearing one free meso position. …”
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Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates by Amin Hadi, Abbas Rastgoo, Maryam Eskandarian, Nooshin Haghighipour, Azam Bolhassani

“…Hence, the use of chemical reagent and mechanical loading simultaneously can be an excellent method in delivering cargoes into DCs and MSCs. …”
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Role of ixodid ticks in tick-borne pathogen spread and circulation in the Belarusian Lakeland by A. A. Osmolovsky, I. A. Subotsina

“….), Babesia spp. and Tick-borne encephalitis virus genetic materials with real-time polymerase chain reaction using the reagent kit for nucleic acid extraction from environmental samples in accordance with the manufacturer’s instruc­tions. …”
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FastContext: A tool for identification of adapters and other sequence patterns in next generation sequencing (NGS) data by E. Viesná, V. Fishman

“…Despite a fairly high level of current knowledge, during the protocol development process researches often have to deal with various kinds of unexpected experiment outcomes, which result either from lack of information, lack of knowledge, or defects in reagent manufacturing. Detection and analysis of service sequences, their distribution and linkage may provide important information for protocol optimization. …”
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Efficacy of bacteriophages with Aloe vera extract in formulated cosmetics to combat multidrug-resistant bacteria in skin diseases by Sodaf A. Maan, Abeer A. Faiesal, Gamar M. Gamar, Noha K. El Dougdoug

“…These bacterial isolates were biochemically identified as S. aureus and P. aeruginosa with probabilities of 98% and 99%, respectively, through VITEK2 system, and were confirmed as multidrug-resistant based on minimum inhibitory concentration test using colorimetric reagent cards. Lytic phages specific to these isolates were isolated, identified through plaque assays, transmission electron microscopy and classified morphologically according to the new International Committee on Taxonomy of Viruses classification as members of the Straboviridae, Drexlerviridae, and Autographiviridae families. …”
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Chemical and Sensory Characterisation of Two Rubus rosifolius (Red Raspberry) Varieties by Theresa F. Rambaran, Camille S. Bowen-Forbes

“…Total phenolic content was determined using the Folin-Ciocalteu reagent assay, and the identification and quantification of anthocyanins were done via HPLC-MS and HPLC-UV, respectively. …”
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Pre-transfusion detection and transfusion therapy of a patient with autoanti-B and cold agglutinin by YANG Linyu, CHEN LI, LI Zhongjun

“…[Methods] Red blood cells of patient were washed with 37℃ physiological saline and treated with sulfhydryl reagent. ABO blood group antigen was detected by tube method and microcolumn gel method. …”
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Leach-SX-EW copper revalorization from overburden of abandoned copper mine Cerovo, Eastern Serbia by Stevanović Z., Antonijević M., Jonović R., Avramović Lj., Marković R., Bugarin M., Trujić V.

“…It was established that for 120 hours of leaching on pH=1.5 without oxidant agents, copper concentration in pregnant leach solutions enriched up to 1.08g/dm3 which was enough for copper extraction from solution with SX-EW treatment. As extraction reagent in SX circuit was used LIX-984N in a kerosene diluent. …”
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Impact of Alkaline Pretreatment to Enhance Volatile Fatty Acids (VFAs) Production from Rice Husk by Qian Fang, Sinmin Ji, Dingwu Huang, Zhouyue Huang, Zilong Huang, Yunyi Zeng, Yu Liu

“…The study investigated the effects of reagent concentration and pretreatment time on protein, carbohydrates, and dissolved chemical oxygen demand (SCOD) dissolution after the pretreatment. …”
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A Sensitive and Rapid Method for Detecting Formaldehyde in Brain Tissues by Xiangpei Yue, Yaoyue Zhang, Wen Xing, Yutong Chen, Chenyang Mu, Zhan Miao, Peichun Ge, Tingting Li, Rongqiao He, Zhiqian Tong

“…Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4-dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature. …”
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Cellular Anti-Inflammatory and Antioxidant Activities of Bamboo Sasa albomarginata Leaf Extract and Its Constituent Coumaric Acid Methyl Ester by Shiori Kojima, Masatoshi Hakamata, Toshimichi Asanuma, Rie Suzuki, Jun-ichi Tsuruda, Takeshi Nonoyama, Yinzhi Lin, Hitomi Fukatsu, Naoki Koide, Kazuo Umezawa

“…ROS production was measured by a fluorescent reagent. Ultrahigh performance liquid chromatography (UHPLC)/MS was used for the ingredient analysis. …”
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How to Efficiently Produce Ultrapure Acids by Damiano Monticelli, Alessio Castelletti, Davide Civati, Sandro Recchia, Carlo Dossi

“…Subboiling distillation has been used since two decades for the purification of analytical grade acids from inorganic contaminants and demonstrated an efficient method to obtain pure acids starting from reagent grade chemicals. Nevertheless, the effect of the subboiling parameters on the purity of the distilled acids has never been methodically investigated. …”
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Esterification of Oleic Acid for Biodiesel Production Using a Semibatch Atomization Apparatus by Marcell S. Deus, Katherine C. O. Deus, Daniel S. Lira, Jackson A. Oliveira, Carlos E. A. Padilha, Domingos F. S. Souza

“…Higher pressure values in the atomization nozzle led to the generation of small oleic acid droplets, which accelerated reagent consumption during the reaction. On the other hand, conversion values were reduced by increasing the oleic acid flow rate. …”
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