%diag_test: a generic SAS macro for evaluating diagnostic accuracy measures for multiple diagnostic tests by Jacques K. Muthusi, Peter W. Young, Frankline O. Mboya, Samuel M. Mwalili

“…Results We tested the macro for quality control purposes by reproducing results from published work on evaluation of multiple types of dried blood spots (DBS) as an alternative for human immunodeficiency virus (HIV) viral load (VL) monitoring in resource-limited settings compared to plasma, the gold-standard. Plasma viral load reagents are costly, and blood must be prepared in a reference laboratory setting by a qualified technician. …”
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Application of a new highly multiplexed amplicon sequencing tool to evaluate Plasmodium falciparum antimalarial resistance and relatedness in individual and pooled samples from Dsc... by Jacob M. Sadler, Alfred Simkin, Valery P. K. Tchuenkam, Isabela Gerdes Gyuricza, Isabela Gerdes Gyuricza, Abebe A. Fola, Kevin Wamae, Ashenafi Assefa, Ashenafi Assefa, Karamoko Niaré, Kyaw Thwai, Samuel J. White, William J. Moss, Rhoel R. Dinglasan, Sandrine Eveline Nsango, Christopher B. Tume, Jonathan B. Parr, Jonathan B. Parr, Jonathan B. Parr, Innocent Mbulli Ali, Jeffrey A. Bailey, Jeffrey A. Bailey, Jonathan J. Juliano, Jonathan J. Juliano, Jonathan J. Juliano, Jonathan J. Juliano

“…Moreover, genotyping of parasites and detection of molecular markers drug resistance in resource-limited regions requires open-source protocols for processing samples, using accessible reagents, and rapid methods for processing numerous samples including pooled sequencing.MethodsPlasmodium falciparum Streamlined Multiplex Antimalarial Resistance and Relatedness Testing (Pf-SMARRT) is a PCR-based amplicon panel consisting of 15 amplicons targeting antimalarial resistance mutations and 9 amplicons targeting hypervariable regions. …”
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Analytical Methods for Atmospheric Carbonyl Compounds: A Review by Xiaoshuai Gao, Xin Zhang, Yan Nie, Jiemeng Bao, Junling Li, Rui Gao, Yunfeng Li, Wei Wei, Xiaoyu Yan, Yongxin Yan, Hong Li

“…For formaldehyde, offline detection was primarily influenced by chemical reagents and reaction conditions, whereas online monitoring relied on sufficiently stable operating environments. …”
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Plant-Based Antigen Production Strategy for SARS-CoV-2 Nucleoprotein and RBD and Its Application for Detection of Antibody Responses in COVID-19 Patients by Katerina Takova, Valeria Tonova, Ivan Minkov, Eugenia S. Mardanova, Nikolai V. Ravin, Stanislav Kotsev, Maria Pishmisheva, Gergana Zahmanova

“…Our research validates the commercial potential of using plants as an expression system for recombinant protein production and their application as diagnostic reagents for serological detection of infectious diseases, hence lowering the cost of diagnostic kits.…”
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The Impact of Mixed Application of Modified Straw Biochar and Organic Fertilizer on Soil Water Retention and Fertility Maintenance in the Loess Hilly Dry Area by ZHANG Peizhen, LUO Juan, YAO Zonglu, CHANG Fangling, HUO Lili, ZHAO Lixin

“…[Methods] The corn straw biochar produced by pyrolysis at 500 ℃ for 2 h under a nitrogen atmosphere was modified by immersion in 1 mol/L H3PO4, NaOH, and NH4Cl reagents, respectively, to obtain different modified corn straw biochars. …”
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Delay of innate immune responses following influenza B virus infection affects the development of a robust antibody response in ferrets by Thomas Rowe, Ashley Fletcher, Melissa Lange, Yasuko Hatta, Gabriela Jasso, David E. Wentworth, Ted M. Ross

“…IAV infection of ferrets to generate these reagents resulted in a strong antibody response, but IBV infection generated weak antibody responses. …”
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Coagulants for water based on activated aluminum alloys by R.G. Sarmurzina, G.I. Boiko, B.K. Kenzhaliyev, U.S. Karabalin, N.P. Lyubchenko, P.V. Kenyaikin, Zh.B. Ilmaliyev

“…The temperature rises from 20 to 25 degree celsius to the specified optimum without heat supply from the outside due to the interaction among reagents. The process is completed in 2–3 hours. The results confirm that aluminum polyoxychloride is an effective coagulant for drinking and wastewater treatment. …”
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Diagnosis of tuberculosis in buffaloes in Argentina: intradermoreaction, compatible lesions and bacteriological culture by Emilia Irina Martínez, Fabiana Cipolini Galarza, Nolly María Monzón, Ana Gabriela Espasandin, José Luis Konrad, Elvira Falzoni, Soledad Barandiaran, Jimena Marfil, Marcela Martínez Vivot, Diana Elina Martínez

“…In Argentina, bovine and buffalo cattle are under a tuberculosis control and eradication program, which includes tuberculin purified protein derivative bovine (PPDB) diagnosis and slaughter of positive reagents. The use of this control tool is controversial in buffaloes. …”
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