Manipulating Intracellular Oxidative Conditions to Enhance Porphyrin Production in <i>Escherichia coli</i> by Bahareh Arab, Murray Moo-Young, Yilan Liu, C. Perry Chou

“…Our approaches included the use of antioxidant reagents and strain engineering. Supplementation with ascorbic acid (up to 1 g/L) increased the UP-III/UP-I ratio from 0.62 to 2.57. …”
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Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation by Beenzu Siamalube, Emmanuel Ehinmitan, Maina Ngotho, Justus Onguso, Steven Runo

“…The transformation efficiency is consistently high, enhancing plasmid uptake while maintaining cell viability, requiring minimal specialized equipment and reagents. The proposed protocol offers significant advantages, including simplicity, reliability, and cost-effectiveness, positioning it as a valuable alternative to traditional techniques in the field of plant biotechnology.…”
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Weight method for determination of soluble β-glucans in barley grain by V. S. Popov, A. V. Konarev, O. N. Kovaleva, N. G. Konkova, V. I. Khoreva

“…The disadvantages of the methods considered include high costs of reagents and equipment, duration of performance, and labor intensity.Results. …”
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Funding of activities of state veterinary services of Russian Federation Subjects by I. M. Klinovitskaya, M. A. Shibayev, A. K. Karaulov

“…The following 4 out of 13 analyzed aspects of activities of the Subjects’ veterinary services were fully funded: staff salaries, anti-epidemic activities, the purchase of reagents and test systems, the implementation of monitoring and screening programmes at the Subject level for contagious animal disease control. …”
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Effortless alkalinity analysis using AI and smartphone technology, no equipment needed, from freshwater to saltwater by Zachary Y. Han, Zihan Zheng, Alan Y. Han, Huichun Zhang

“…Specifically, we employed low-cost commercial reagents to generate color changes in response to alkalinity levels in various freshwater and saltwater samples. …”
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Green and High Throughput Assay Using 96-Microwell Base to Determine Metformin Hydrochloride in the Tablet Dosage Form by Mohammed Alqarni, Atheer Alshehri, Bayan Almalki, Refah Althumali, Maram Alghamdi, Rawan Alqahtani, Safia G. Alotibi, Ali Alqarni, Adel H. Awad, Ibrahim A. Naguib

“…The use of the 96-microwell-plate method is superior to the existing method in terms of simplicity of the procedure, the low economic cost, and its consumption of low amounts of reagents and organic ethanol solvent, making it an environmentally friendly method. …”
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Scoping review of the applications of peptide microarrays on the fight against human infections. by Arthur Vengesai, Maritha Kasambala, Hamlet Mutandadzi, Tariro L Mduluza-Jokonya, Takafira Mduluza, Thajasvarie Naicker

“…Peptide microarray arrays were also used for T-cell linear epitope mapping which may provide more information for the design of peptide-based vaccines and for the development of diagnostic reagents.…”
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Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis by Parsa Kamali, Gregory Fairn

“…Unfortunately, many macrophage cell lines are refractory to most reagents used for transient transfections. Alternative transient approaches, such as electroporation or transduction with lentiviral vectors, typically cause cell death (electroporation) or can be time-consuming to generate numerous lentivirus when using different genes of interest. …”
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Methodological aspects of using NIR spectroscopy to assess biochemical indicators in barley grain by V. S. Popov, T. V. Shelenga, O. N. Kovaleva, V. I. Khoreva

“…The proposed method helps to preserve valuable source material, increases labor efficiency, and does not require chemical reagents. Scanning each sample makes it possible to obtain data for several indicators at once, with a specified replication and standard deviation.…”
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Plant-Mediated Green Synthesis of Ag NPs and Their Possible Applications: A Critical Review by Darbin Kumar Poudel, Purushottam Niraula, Himal Aryal, Biplab Budhathoki, Sitaram Phuyal, Rishab Marahatha, Kiran Subedi

“…Although these methods produce nanoparticles with high purity and well-defined morphology, it is critical to develop cost-effective, energy-efficient, and facile route, such as green synthesis; it suggests the desirable use of renewable resources by avoiding the use of additional solvents and toxic reagents in order to achieve the ultimate goal. However, each method has its pros and cons. …”
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Ultrasound-Assisted Extraction of Micro- and Macroelements in Fruit Peel Powder Mineral Supplement for Osteoporosis Patients and Their Determination by Flame Atomic Absorption Spec... by Muhammad Saleem, Arjumand Iqbal Durani, Rima D. Alharthy, Mahmood Ahmed, Muhammad Imtiaz Shafiq, Abdul Hameed, Syed Mubasher Hussain, Waqas Bashir

“…The proposed UAE method was reliable, efficient, and advantageous over the conventionally employed acid digestion method with regard to less consumption of reagents and short analysis time for the determination of micro- and macroelements in fruit peel powder.…”
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Cytotoxic Activity of Bisphosphonic Derivatives Obtained by the Michaelis–Arbuzov or the Pudovik Reaction by Zsuzsanna Szalai, Janka Bednárik, Boldizsár Szigfrid Tóth, Angéla Takács, Szilárd Tekula, László Kőhidai, Konstantin Karaghiosoff, László Drahos, György Keglevich

“…<b>Methods:</b> Methylenebisphosphonates may be obtained by the Michaelis–Arbuzov reaction of suitably α-substituted methylphosphonates and trialkyl phosphites or phosphinous esters, while the hydroxy-methylene variations are prepared by the Pudovik reaction of α-oxophosphonates and different >P(O)H reagents, such as diethyl phosphite and diarylphosphine oxides. …”
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Rapid assessment of the main economic value indicators in lupine flour samples using infrared spectroscopy by V. S. Popov, A. V. Salikova, I. N. Perchuk, N. G. Konkova, G. P. Egorova, M. A. Vishnyakova, T. V. Shelenga

“…The analysis does not require reagents and is safe.…”
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A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei by Mitsuru Nakase, Jeewan Thapa, Vanaabaatar Batbaatar, Ochirbat Khurtsbaatar, Batchuluun Enkhtuul, Jugderkhorloo Unenbat, Baasansuren Lkham, Sachiho Fujita, Ai Koshikawa, Apichai Tuanyok, Vannarat Saechan, Hideaki Higashi, Kyoko Hayashida, Yasuhiko Suzuki, Chie Nakajima, Takashi Kimura

“…These obstacles make it difficult to diagnose B. mallei and B. pseudomallei infections in areas where reagents and equipment are difficult to procure. To solve this problem, we developed an easy and ready-to-use dried-format diagnostic tool based on loop-mediated isothermal amplification (LAMP) method. …”
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Antimony recovery from recycled terminals of lead-acid batteries with Na2CO3 and SiC after firstly SsB2O3 formation by Jiménez-Lugos J.C., Sánchez-Alvarado R.G., Cruz-Ramírez A., Romero-Serrano J.A., Hernández-Ramírez A., Rivera-Salinas J.E.

“…The antimony trioxide was reduced by C present in reagents while silicon and sodium formed a slag phase. …”
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Development and validation of a LAMP-based method for rapid and reliable detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald by Moutoshi Chakraborty, Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Shamsul Arafin Bhuiyan, Simon Strachan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, Rebecca Ford, Rebecca Ford

“…The presence of irregular symptoms makes traditional phenotypic detection difficult, and molecular methods necessitate costly equipment, labor, and extended sample-to-answer processing times.MethodsThis study introduces an innovative rapid DNA isolation method requiring no reagents, combined with an isothermal amplification-based assay for efficient detection of Xalb DNA in sugarcane xylem sap, leaf tissue, and meristematic tissue samples. …”
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Selection of an optimal method for screening the collection of narrow-leaved lupine held by the Vavilov Institute for the qualitative and quantitative composition of seed alkaloids by A. V. Kushnareva, Т. V. Shelengа, I. N. Perchuk, G. P. Egorova, L. L. Malyshev, Yu. A. Kerv, A. L. Shavarda, M. A. Vishnyakova

“…Qualitative and quantitative indices were compared when target compounds had been extracted with multicomponent mixtures and individual reagents (chloroform, methanol, etc.) and the extracts analyzed using gas chromatography-mass spectrometry. …”
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Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification by Christian Diamant Mossoro-Kpinde, Ralph-Sydney Mboumba Bouassa, Mohammad-Ali Jenabian, Serge Tonen Wolyec, Leman Robin, Mathieu Matta, Jean de Dieu Longo, Gérard Grésenguet, Laurent Andreoletti, Laurent Bélec

“…We evaluated the performances of Amplix real-time PCR platform developed by Biosynex (Strasbourg, France), combining automated station extraction (Amplix station 16 Dx) and real-time PCR (Amplix NG), for quantifying plasma HIV-1 RNA by lyophilized HIV-1 RNA-based Amplix reagents targeting gag and LTR, using samples from HIV-1-infected adults from Central African Republic. …”
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Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay by Bhagya Deepachandi, Sudath Weerasinghe, Thisira Priyantha Andrahennadi, Nadira D. Karunaweera, Nadeeja Wickramarachchi, Preethi Soysa, Yamuna Siriwardana

“…Concentrations of unknown samples were calculated using a standard curve prepared using a standard series of bovine serum albumin (BSA). The optimized reagents were 2 N NaOH (sodium hydroxide), 2% Na2CO3 (sodium carbonate), 1% CuSO4 (copper sulfate), 2% KNaC4H4O6 (potassium sodium tartrate), and 2 N Folin and Ciocalteu’s phenol. …”
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Justification and selection of a method for restricting water inflow to horizontal wells in low-permeability terrigenous reservoir conditions using the example of the YuS2 formatio... by Olga V. Savenok, Nailya Kh. Zharikova, Elizaveta P. Chuykova, Alexander E. Verisokin, Mahmoud Hadid, Larisa V. Povarova, Denis S. Kalashnikov, Anna S. Domanova

“…Subject. Various chemical reagents for isolating water inflow and methods for determining the suitability of a well for water isolation work. …”
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