Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering by Man-Il Huh, Kyoung-Pil Lee, Jeongho Kim, Soojin Yi, Byeong-Ung Park, Hong Kyun Kim

“…To establish an optimized and standardized protocol for the development of optimal scaffold for bioengineering corneal substitutes, we used femtosecond laser to process human corneal tissue into stromal lenticules and studied to find the most efficient decellularization method among various reagents with different tonicities. Methods. The decellularization efficacy of several agents (0.1%, 0.25%, and 0.5% of Triton X-100, SDS, and trypsin-EDTA (TE), resp.) with different tonicities was evaluated. …”
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Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes by Yan Zhang, Xiangming Fang, Fen Shuang, Guoxun Chen

“…Primary hepatocytes from Sprague-Dawley rats were isolated, cultured and treated with insulin in the presence or absence of the indicated reagents over time. The mRNA levels of indicated genes were determined using real-time PCR. …”
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An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts by Aruto Takano, Dennis V. Umali, April H. Wardhana, Dyah H. Sawitri, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi

“…This ultra-simplified protocol for preparation of PCR template, which does not require expensive reagents or equipment, will significantly enhance the sensitive and efficient molecular identification of Eimeria. …”
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Ultra-high-resolution Mass Spectra of Dissolved Organic Matter in the Absorption Mode by Rong QIN, Lun YANG, Qing-long FU

“…Moreover, the absorption mode is mainly favorable to the number of identified peaks for DBPs but has limited effects on decreasing the peak intensity deviation of DBPs peaks due to their low intensity at the environmental levels of chlorination reagents. The results highlight the great potential of the absorption mode technology based on FT-ICR MS for the non-targeted analysis of DOM and emerging pollutants, including DBPs.…”
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A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies by Huifang Chen, Zehong Zou, Ailin Tao

“…These mAbs can, therefore, serve as quantitative diagnostic reagents for peanut and peanut product risk assessment.…”
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Standartization of Forensic Activities and Certification of Expert Laboratories as a Factor for the Development of Forensic Examination by I. V. Kuchynska

“…Considerable attention has been paid to the quality of standard samples, reagents and materials. The emphasis has been placed on the need to participate in proficiency testing programs based on the results of interlaboratory comparative tests as an effective way to monitor the activities of the forensic laboratory. …”
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Application of Response Surface Methodology (RSM) for Simultaneous Optimization of Kinetic Parameters Affecting Gold Leaching in Thiosulfate Based Media: A Statistical Approach by Nomsa P. Baloyi, Joseph M. Nseke, Mamookho E. Makhatha

“…Moreover, with global economic factors causing a decline in gold prices, it is crucial that novel hydrometallurgical methods of extracting gold minimise operational costs by using low-priced reagents such as thiosulfate. In the current study, the response surface methodology (RSM) approach is used to optimize the kinetic factors (temperature and copper, ammonia, and thiosulphate concentration) affecting gold leaching. …”
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Green synthesis of silica-coated gold nanoparticles employing femtosecond laser, solid targets, and water by N. G. P. Machado, M. P. Raele, E. Jimenez-Villar, W. de Rossi

“…This study introduces a novel approach for synthesizing silica-coated gold nanoparticles (AuNP@SiO₂) using femtosecond laser ablation in water, eliminating the need for chemical reagents. The process involves three key laser-based steps: Si ablation, SiNP@SiO₂ fragmentation, and Au ablation, all conducted in a liquid environment. …”
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Broad-Purpose Solutions of N-Chlorotaurine: A Convenient Synthetic Approach and Comparative Evaluation of Stability and Antimicrobial Activity by Bohdan Murashevych, Dmitry Girenko, Iryna Koshova, Ganna Maslak, Konstantin Burmistrov, Dmytro Stepanskyi

“…Considering the stability and compactness of the initial chlorine-active polymers, as well as the possibility of their multiple regeneration, the developed method can form the basis of the technology for obtaining multifunctional NCT solutions for medical purposes with the desired physical and chemical properties without using special equipment or specific reagents.…”
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Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay by Lei Ma, Xueping Wang, Mingliang Zhang, Mengjie Zhu

“…RPA and CRISPR/Cas12a reagents were added to the bottom and lid of the test tube at once, allowing the detection process to occur within a single reaction tube. …”
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Simultaneous Determination of Malachite Green, Chloramphenicols, Sulfonamides, and Fluoroquinolones Residues in Fish by Liquid Chromatography-Mass Spectrometry by Yongping Chen, Sudong Xia, Xianqin Han, Zhiru Fu

“…The selection of extraction reagents was optimized using different types of microfiltration membrane, mobile phase, and LC column. …”
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Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products by Oleh Smutok, Maria Karkovska, Halyna Smutok, Mykhailo Gonchar

“…The main advantages of the proposed method when compared to the LDH-based routine approaches are a higher sensitivity (2.0 μM of L-lactate), simple procedure of analysis, usage of inexpensive, nontoxic reagents, and small amount of the enzyme. Enzymatic oxidation of L-lactate catalyzed by flavocytochrome b2 and coupled with formazan production from nitrotetrazolium blue was shown to be used for L-lactate assay in food samples. …”
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Design and fabrication of novel microfluidic-based droplets for drug screening on a chronic myeloid leukemia cell line. by Niloofar Jaafari, Amir Asri Kojabad, Rima Manafi Shabestari, Majid Safa

“…Using microfluidic chips, single-cell analysis can be conducted more effectively, requiring fewer reagents and maintaining biocompatibility. Due to the chip's ability to manipulate small volumes of fluid, high-throughput screening assays can be developed that are both miniaturized and automated. …”
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Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility by Chenyue Qian, Heming Guo, Xiaohong Chen, Aiming Shi, Sicheng Li, Xin Wang, Jie Pan, Chen Fang

“…Genotyping of 8 selected SNPs of PD-1 and PD-L1 was carried out by the pyrosequencing PSQ 24 System using PyroMark Gold reagents (QIAGEN). Results. SNP rs4143815 in PD-L1 was significantly associated with T1DM. …”
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Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs by Anna Schade, Paula Müller, Evgenya Delyagina, Natalia Voronina, Anna Skorska, Cornelia Lux, Gustav Steinhoff, Robert David

“…We found that all tested transfection reagents had high miR uptake rates (yielded over 60%) and no significant cytotoxic effects. …”
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‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…The <i>mScarlet-I</i> gene was introduced into the pYFRed for the screening of the positive recombinants by the unaided eye without the need for additional reagents/equipment. pYFRed is easy to construct in an ordinary laboratory, which facilitates researchers to develop their cloning vector without purchasing commercial cloning vectors.…”
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Characterization of combined blast- and fragments-induced synergetic damage in polyurea coated liquid-filled container by Chen Tao, Chong Ji, Xin Wang, Juan Gu, Yuting Wang, Changxiao Zhao

“…Liquid-filled containers (LFC) are widely used to store and transport petroleum, chemical reagents, and other resources. As an important target of military strikes and terrorist bombings, LFC are vulnerable to blast waves and fragments. …”
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Controversial Roles of Regenerating Family Proteins in Tissue Repair and Tumor Development by Luting Yu, Qingyun Wu, Shenglong Jiang, Jia Liu, Junli Liu, Guoguang Chen

“…These theoretical foundations facilitate the subsequent development of diagnostic reagents and therapeutic drugs targeting Reg proteins.…”
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Elevated Concentration of Defensins in Hepatitis C Virus-Infected Patients by Ehab H. Mattar, Hussein A. Almehdar, Abdullah A. AlJaddawi, Isam ElDin M. Abu Zeid, Elrashdy M. Redwan

“…The obtained results were calibrated against kit standard reagents. Total defensin concentrations in HCV-infected patients were significantly higher (2- to 105-fold) compared to healthy individuals. …”
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Albumin as an Effective Auxiliary Agent for the Enriched Extraction of Anthraquinones and Curcumin from Plant Matrices by Chiara Collevecchio, Salvatore Genovese, Francesco Epifano, Lorenzo Marchetti, Serena Fiorito

“…The easy-to-handle procedures, readily accessible facilities, and the employment of cheap substrates and reagents represent the most evident advantages of the methodology described herein.…”
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