Coupling Strategies for the Synthesis of Peptide-Oligonucleotide Conjugates for Patterned Synthetic Biomineralization by Joshua D. Carter, Thomas H. LaBean

“…The yield of four coupling reagents is evaluated, two reagents in water, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) and DMTMM (4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium chloride), and two in dimethylformamide (DMF), PyBOP ((Benzotriazol-1-yloxy) tripyrrolidinophosphonium hexafluorophosphate) and HBTU (O-benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate), while the oligonucleotide fragment is either in solution or immobilized on DEAE. …”
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Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA by Momchilo Vuyisich, Ayesha Arefin, Karen Davenport, Shihai Feng, Cheryl Gleasner, Kim McMurry, Beverly Parson-Quintana, Jennifer Price, Matthew Scholz, Patrick Chain

“…The NEBNext Ultra reagents enable high quality resequencing and de novo assembly of a variety of bacterial genomes when using 100 ng of input genomic DNA. …”
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Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge by M. Biddle, P. Stylianou, M. Rekas, A. Wright, J. Sousa, D. Ruddy, M. I. Stefana, K. Kmiecik, A. Bandrowski, R.A. Kahn, C. Laflamme, E. M. Krockow, H.S. Virk

“…Antibodies are one of the most important reagents used in biomedical and fundamental research, used to identify, and quantify proteins, contribute to knowledge of disease mechanisms, and validate drug targets. …”
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Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays by Kamar Shayah, Joseph Shenekji, Lana Karnoub, Sana Wohbeh, Hagar Hamsho

“…The suggested frugal method relies on providing a new solution for extracting DNA from human saliva without the need for enzymes or high grade reagents or columns, which makes suitable for low budget laboratories in developing countries to manufacture it locally, it uses a phase separation with technical skills to extract the DNA from the saliva achieving safety by not using hazardous reagents, and it is a sustainable eco-friendly method that gives decent yield of good quality DNA in ~20 minutes to be used in PCR applications with a cost that is estimated to be 10 times cheaper than current used methods.…”
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Comparison of Microfluidic Synthesis of Silver Nanoparticles in Flow and Drop Reactors at Low Dean Numbers by Konstantia Nathanael, Nina M. Kovalchuk, Mark J. H. Simmons

“…Aqueous solutions of reagents were supplied through two different channels merging just before the drops were formed. …”
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Simple Instrumental and Visual Tests for Nonlaboratory Environmental Control by L. P. Eksperiandova, S. V. Khimchenko, N. A. Stepanenko, I. B. Shcherbakov

“…As the test tools, the following ones are worked out: polyurethane foam tablets with sorbed colored complexes, the two-layer paper sandwich packaged in slide adapter and saturated by reagents, and polyethylene terephthalate blister with dried reagents. …”
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Adsorption of Zn and Cd Ions on Vermiculite in Buffered and Unbuffered Aqueous Solutions by Xiaofu Wu, Hailan Zhou, Fang Zhao, Chong Zhao

“…Studies of Zn 2+ and Cd 2+ ion adsorption onto vermiculite from buffered and unbuffered aqueous solutions were conducted in order to compare their ion adsorption behaviours in different systems. The use of buffer reagents had a strong and negative effect on the adsorption of the metal ions under study. …”
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New insights into the DNA extraction and PCR amplification of minute ascomycetes in the genus Laboulbenia (Pezizomycotina, Laboulbeniales) by Warre Van Caenegem, Danny Haelewaters

“…We found that halving the reagents used in the single cell-based extraction kit did not significantly affect the probability of successful DNA extraction. …”
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Optimization of chemical transfection in airway epithelial cell lines by Tony J. F. Guo, Wan Yi Liang, Gurpreet K. Singhera, Jasmine Memar Vaghri, Janice M. Leung, Del R. Dorscheid

“…Results We used six common and/or commercially available reagents with varying chemical compositions: Lipofectamine 3000 (L3000), FuGENE HD, ViaFect, jetOPTIMUS, EndoFectin, and calcium phosphate. …”
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Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts by Supriya Sharma, Gaurav Pande, Mohit Kumar Rai, Vikas Agarwal

“…However, establishing primary cultures is challenging and despite availability of many protocols for primary cell cultures reproducibility is lacking either due to cumbersome protocol, need for special reagents, and requirement of training. Herein, we present our simplistic least manipulative protocols for establishing primary human dermal fibroblasts, human pancreatic stellate cells, and human peritoneal fibroblasts that may be followed by other research laboratories for in vitro studies.…”
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Phlorofucofuroeckol-A: A Natural Compound with Potential to Attenuate Inflammatory Diseases Caused by Airborne Fine Dust by Eun-Gyeong Lee, Sung-Kun Yim, Sang-Min Kang, Byung Jae Ahn, Chang-Kwon Kim, Mina Lee, Dongseob Tark, Gun-Hee Lee

“…We aimed to identify potential seaweed anti-inflammatory bioactive reagents and determine their effects on systemic inflammatory responses induced by FD particles. …”
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Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens by Patricia Cuenca-Lara, Patricia Cuenca-Lara, Miriam Blay-Benach, Miriam Blay-Benach, Zoraida Cervera, Zoraida Cervera, Cristian Melgarejo, Cristian Melgarejo, Julia Moraleda, Julia Moraleda, Iker A. Sevilla, Joseba M. Garrido, Mahavir Singh, Gareth J. Jones, Bernat Pérez de Val, Bernat Pérez de Val

“…However, the use of reagents based on the defined antigens eliminated or reduced the vaccine-induced diagnostic interferences in all groups. …”
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Nucleophilic addition to carbonyl groups from qualitative to quantitative computational studies. A historical perspective by Eisenstein, Odile

“…The mechanism of this reaction for various nucleophiles (especially the early main group hydride and the Grignard reagents) and the various noncyclic and cyclic aldehydes or ketones has fascinated computational chemists for nearly 40 years. …”
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Can antibodies be “vegan”? A guide through the maze of today’s antibody generation methods by Stefan Dübel

“…Nevertheless, the use of antibody reagents that are poorly characterized has led to the publication of false or misleading results. …”
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Discovery and Verification of Gelsolin as a Potential Biomarker of Colorectal Adenocarcinoma in a Chinese Population: Examining Differential Protein Expression using an Itraq Label... by Nai-Jun Fan, Chun-Fang Gao, Chang-Song Wang, Jing-Jing Lv, Guang Zhao, Xin-Hua Sheng, Xiu-Li Wang, Dong-Hui Li, Qing-Yin Liu, Jian Yin

“…This article describes a novel technology that uses isotopic reagents to tag selected proteins that show a consistent pattern of differential expression in CRC.…”
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Study on Designing Sulfur Containing Bifunctional Building Block with Improved Refractive Index by G. S. Jha, P. Chhabra, G. Suri, M. Tyagi, P. Arora, G. Seshadri, R. K. Khandal

“…The reaction is highly exothermic and the effect of temperature, rate of addition of reagents and reaction time are important factors affecting the formation of diol. …”
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Microwave-assisted heating in a novel thin film-liquid spinning coaxial reactor by Guennadi A. Kouzaev

“…It is hypothesized that the acceleration of the conversation rate of chemical reactions is due to the excitation of micro-vortices for better mixing and the direct application of microwaves to a mix of polar reagents within the narrow gap. This study presents initial experimental results of microwave liquid heating, hydrodynamics, and mechanics in this reactor.…”
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Epidemiology of Brucellosis in Small Ruminants of Rural and Peri-Urban Areas of Multan, Pakistan by Mian Muhammad Awais, Bakhtawar Khadim, Masood Akhtar, Muhammad Irfan Anwar, Gohar Khadim, Abdul Sammad Ali Khan Shirwany, Halil Selcuk Biricik, Abdul Razzaq, Muhammad Sibtain Bhatti

“…For this purpose, sera samples (n = 392) of small ruminants were collected and subjected to preliminary screening using commercially available RBPT reagents followed by serodetection of brucellosis using multispecies i-ELISA kit (ID.vet, France). …”
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Comparative study of PCR test kits for ASFV DNA detection by M. S. Krasnikova, S. P. Yatsentyuk, M. B. Bryusova, A. D. Kozlova, М. А. Gergel

“…The paper presents comparative test results of 12 domestically produced diagnostic kits/PCR test systems for DNA detection of the African swine fever virus with regard to the following parameters: completeness and correctness of instructions for use; labeling and package contents; convenience of using the kit; shelf life stability of reagents; stability of reagents after transportation and repeated freezing – thawing; batch-to-batch repeatability; sensitivity of various test materials and specificity of kits. …”
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Quantifying LAGA mutated mouse IgG2a monoclonal antibody with a rapid pepsin digestion enabled immunoaffinity LC/MS/MS assay by Linlin Dong, Susan Chen, Konstantin Piatkov, Dong Wei, Mark G. Qian

“…A sensitive and specific bioanalytical method was required to measure the exposure of a LAGA-mutated surrogate mouse IgG2a monoclonal antibody in mouse plasma, but the lack of highly specific reagents for the LAGA mutant hindered the development of a ligand-binding assay. …”
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