Cytogenetic factors decreasing the fertility of pollen and cobs during clogging of tetraploid maize with triploid grains (<i>Zea mays</i> L.) by E. B. Khatefov, A. A. Grushin, V. N. Boyko

“…Metaphase plates of maize roots were stained with Schiff’s reagent according to Feulgen and pollen grains were stained with Lugol’s solution.Results. …”
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Fluorescence Polarization Immunoassay for Rapid, Sensitive Detection of the Herbicide 2,4-Dichlorophenoxyacetic Acid in Juice and Water Samples by Liliya I. Mukhametova, Marya K. Kolokolova, Ivan A. Shevchenko, Boris S. Tupertsev, Anatoly V. Zherdev, Chuanlai Xu, Sergei A. Eremin

“…Monoclonal antibodies (MAb) obtained against 2,4-D were used as a recognition reagent. The kinetics of the interaction of MAb and tracers were studied, and the kinetic parameters of their binding were calculated. …”
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Human umbilical cord derived-mesenchymal stem cells alleviate lipid metabolism disturbances of hepatocytes via mitochondrial transport by Jing Xue, Xiangnan Hu, Suying Xia, Pengfei Ren, Aihong Wang

“…Evaluate lipid deposition in HepG2 cells using oil red staining. Use a reagent kit to detect the triglyceride content in HepG2 cells. …”
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Derivatization of Levoglucosan for Compound-Specific δ13C Analysis by Gas Chromatography/Combustion/Isotope Ratio Mass Spectrometry by Dongling Zhang, Swe Swe Aung, Yong Han, Yanlin Zhang, Yingjun Chen, Hongli Li, Feifei Yu, Yangjun Wang, Jialiang Feng, Wu Wang

“…The recommended reaction temperature is 70°C; the reaction time is 60 min for MBA and 120 min for MSTFA derivatization; and the molar ratio of levoglucosan : MBA : MSTFA is 1 : 1: 100 and 1 : 1: 120 and the reagent volume ratio of MSTFA : pyridine is between 1 : 3 and 1 : 4. …”
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Treatment of a mutant KRAS lung cancer cell line with polyisoprenylated cysteinyl amide inhibitors activates the MAPK pathway, inhibits cell migration and induces apoptosis. by Matthew D Gregory, Kweku Ofosu-Asante, Jassy Mary S Lazarte, Pablo E Puente, Nada Tawfeeq, Nadine Belony, Yong Huang, Ite A Offringa, Nazarius S Lamango

“…An analysis of cells treated with the fluorescently labeled active caspase 3/7 irreversible inhibitor, CaspaTagTM Caspase-3/7 in situ reagent revealed a 124% increase in active caspase at 3 μM over controls. …”
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Synthesis and Evaluation of N-[1-(((3,4-Diphenylthiazol-2(3H)-ylidene)amino)methyl)cyclopentyl]acetamide Derivatives for the Treatment of Diseases Belonging to MAOs by Gülhan Turan-Zitouni, Aouatef Tabbi, Weiam Hussein, Abdullah Burak Karaduman, Begüm Nurpelin Sağlık, Yusuf Özkay

“…The inhibitory activity of compounds (4a-4i) against hMAO-A and hMAO-B enzymes was elucidated by using in vitro fluorometric method using Amplex Red® reagent. In the hMAO-A inhibition assay, compounds 4a, 4b, 4c, and 4i exhibited similar activity with standard drug moclobemide (IC50 = 6.061 ± 0.262 µM) with IC50 values of 7.06 ± 0.18 µM, 6.56 ± 0.20 µM, 6.78 ± 0.15 µM, and 7.09 ± 0.17 µM, respectively. …”
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A prospective, cross-sectional study to establish age-specific reference intervals for neonates and children in the setting of clinical biochemistry, immunology and haematology: th... by Susan M Donath, Monsurul Hoq, Vicky Karlaftis, Susan Mathews, Janet Burgess, John Carlin, Paul Monagle, Vera Ignjatovic

“…However, the accuracy of a reference interval is dependent on the selected reference population, and in paediatrics, the ability of the reference interval to reflect changes associated with growth and age, as well as sex and ethnicity. Differences in reagent formulations, methodologies and analysers can also impact on a reference interval. …”
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A Case of Nonfunctioning Pituitary Carcinoma That Responded to Temozolomide Treatment by Haruko Morokuma, Takao Ando, Takuya Hayashida, Ichiro Horie, Naoko Inoshita, Fumi Murata, Ikuko Ueki, Kan Nakamura, Misa Imaizumi, Toshiro Usa, Atsushi Kawakami

“…Pituitary carcinomas commonly produce either PRL or ACTH, but some do not produce pituitary hormones. The alkylating reagent temozolomide (TMZ) was recently shown to be effective as a treatment for pituitary carcinoma. …”
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<i>In vitro</i> assay of biological activity of a national preparation of macrophage activating factor (GcMAF-RF) by E. V. Levites, S. S. Kirikovich, E. V. Dolgova, A. S. Proskurina, G. S. Ritter, А. A. Ostanin, E. R. Chernykh, S. S. Bogachev

“…The level of NO production was estimated by the accumulation of nitrogen monoxide in the culture supernatants of peritoneal macrophages by the colorimetric method using the Griess reagent. It was shown that GcMAF-RF multiplies the phagocytic activity of macrophages and significantly increases their production of nitrogen monoxide. …”
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Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency by Salvatore Mottola, Alessandra Del Bene, Vincenzo Mazzarella, Roberto Cutolo, Ida Boccino, Francesco Merlino, Sandro Cosconati, Salvatore Di Maro, Anna Messere

“…This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usage compared to conventional manual SPPS protocols. …”
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Antioxidant and anti-inflammatory function of walnut green husk aqueous extract (WNGH-AE) on human hepatocellular carcinoma cells (HepG2) treated with t-BHP. by Zheng-Qiang Li, Yu-Ting Gao, Chen-Fa Zhao, Rui An, Yan-Lv Wu, Zhi-Pang Huang, Ping Ma, Xu Yang, Rong She, Xiao-Yan Yang

“…The total phenols, total polysaccharides, and total flavonoids in WNGH-AE were analyzed using the Folin-Ciocalteu's reagent, the phenol-sulfuric acid method, and the spectrophotometry, respectively. …”
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The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research by Yun Liu, Ting‐Ting Feng, Wei Tong, Zhi Guo, Xia Li, Qi Kong, Zhi‐Guang Xiang

“….), the attached cells were washed with PBS and harvested in Trizol reagent. Then The harvest is subjected to transcriptome sequencing. …”
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miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia by Mete Ozkurt, Thomas Hellwig-Bürgel, Reinhard Depping, Selda Kadabere, Rumeysa Ozyurt, Abdullah Karadag, Nilüfer Erkasap

“…We transfected the miR663 group with the mimic-miR663 (30 pmol) for 24 hrs; other groups were treated with a transfection reagent followed by treatment of TNF-α for 24 hrs; miR663 groups were treated with TNF-α for 24 hrs; and the control group was incubated with normal medium. …”
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Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae by Tjalling Gijsbert Tjalsma, Yannick Patrice Didion, Ziran Su, Magdalena Malankowska, Pablo Torres-Montero, José Luis Martínez, Manuel Pinelo

“…DES lidocaine:decanoic acid 1:3 resulted in a protein yield of 2.45 (±0.03) mg/mL, which outperformed a method using a standard lysis reagent that resulted in 2.33 (±0.04) mg/mL. It was concluded that the underlying linkages between HBA and HBD play a key role because different HBA and HBD combinations have a bearing on whether protein release is successful.…”
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Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products by Mingfei Pan, Liping Hong, Jingying Yang, Xiaoqian Xie, Kaixin Liu, Shuo Wang

“…Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. …”
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Development of High-performance Proton Transfer Reaction Time-of-Flight Mass Spectrometer by Chun-guang XIE, Ji-yang ZHANG, Tong YANG, Yu-liang HUANG, Shu-guang XIE, Hui ZHU, Jing-xuan SUN, Ping CHENG

“…Based on the proton affinity of the reaction reagent ions, the system can analyze the majority of VOCs while excluding interference from air components. …”
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Cell-free DNA predicts all-cause mortality of sepsis-induced acute kidney injury by Feixiang Xu, Xiao Tan, Jianli Wang, Su Lu, Hailin Ding, Mingming Xue, Yumei Chen, Sheng Wang, Jie Teng, Yiqin Shi, Zhenju Song

“…CfDNA was extracted from serum and quantified using Quant-iT PicoGreen dsDNA Reagent.Results Overall survival was significantly lower in the high cfDNA group than in the low cfDNA group (Log-Rank p = 0.012). …”
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Carbon-carbon triple bond cleavage and reconstitution to achieve aryl amidation using nitrous acid esters by Zi-Ying Wang, Shoujun Wang, Nan-Nan Dai, Yao Xiao, Yu Zhou, Wen-Chan Tian, Dongru Sun, Qiang Li, Yong Wang, Wen-Ting Wei

“…Nitrous acid ester serves as both a radical source and a hydrogen atom transfer (HAT) reagent to functionalize and utilize the two carbon atoms of the C–C triple bond. …”
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Blockade of Erythropoietin-Producing Human Hepatocellular Carcinoma Receptor B1 in Spinal Dorsal Horn Alleviates Visceral Pain in Rats by Chen-Li Sun, Cheng-Wen Li, Nong He, Yuan-Zhang Tang, Xiu-Liang Li, Fu-Shan Xue, Jia-Xiang Ni

“…Adult male Sprague-Dawley rats were randomly divided into three groups receiving different treatments (n = 16 per group): intracolonic vehicle (control group), intracolonic 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) (TNBS group), and intracolonic TNBS and intrathecal EphB1 receptor blocking reagent (TNBS + EphB2-Fc group). Visceral hyperalgesia was evaluated with quantification of visceral pain threshold induced by colorectal distention. …”
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Development of a cell-penetrating peptide-based nanocomplex for long-term delivery of intact mitochondrial DNA into epithelial cells by Kyrie Wilson, Charles Holjencin, Hwaran Lee, Balasubramaniam Annamalai, Masaaki Ishii, Jeremy L. Gilbert, Andrew Jakymiw, Bärbel Rohrer

“…More specifically, the novel therapeutic nanoparticle complex used in our study was generated by combining a cell-penetrating peptide (CPP) with purified mtDNA, in conjunction with a mitochondrial targeting reagent. The generated nanoparticle complexes were found to be taken up by cells and localized to mitochondria, with exogenous mtDNA retention/maintenance, along with mitochondrial RNA and protein production, observed in mitochondria-depleted ARPE-19 cells at least 4 weeks following a single treatment. …”
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