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    Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model by Shuyi Liu, Wei Si, Zhengbo Wang

    Published 2025-03-01
    “…We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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    Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

    Published 2025-03-01
    “…By preserving tumor architecture and heterogeneity, this model is applicable for evaluating tumor characteristics, immune interactions, and treatment efficacy in translational cancer research. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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    A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

    Published 2025-03-01
    “…We then detail procedures for performing an adhesion assay.For complete details on the use and execution of this protocol, please refer to Kumari et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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  13. 573

    Estimation of Fresh and Hardened Properties of Self-Compacting Concrete by Optimized Radial Basis Function Methods by David Cadasse, Antonio Fontana

    Published 2022-10-01
    “…Most of the published literature on concrete containing fly ash was limited to predicting the hardened concrete properties. …”
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    A Novel Hybrid Radial Basis Function Method for Predicting the Fresh and Hardened Properties of Self-Compacting Concrete by Zhangabay Nurlan

    Published 2022-04-01
    “…It is observed from the published literature that there were so few studies concentrating on predicting both fresh and hardened properties of self-compacting concrete (SCC). …”
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    Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing by Diane Delobel, Hiromi Nishiyori-Sueki, Ilaria Nisoli, Hideya Kawaji, Pauline Robbe, Piero Carninci, Hazuki Takahashi

    Published 2025-03-01
    “…We describe steps for sequencing, automated data processing, and complete analytical framework ensuring CAGE operability for the determination of TSS and enhancers on the ever-evolving Illumina sequencing platforms. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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