Protocol for assessing T cell receptor-mediated human T cell cytotoxicity by Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao

“…This protocol can be applied to small numbers of effector cells isolated from patient samples.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy by Jian Zhou, Fengling Du, Fan Zhang, Xianhui Zhang, Yuanyuan Wu, Lifang Zhang, Ping Liao, Tianqi Tu, Jianhua Peng, Tao Li, Yong Jiang

“…We then detail procedures for the preparation of a coverslip with a customized titanium ring and cranial window for two-photon microscopy scanning.For complete details on the use and execution of this protocol, please refer to Zhou et al. 1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for functional screening of CFTR-targeted genetic therapies in patient-derived organoids using DETECTOR deep-learning-based analysis by Mattijs Bulcaen, Ronald B. Liu, Kasper Gryspeert, Sam Thierie, Anabela S. Ramalho, François Vermeulen, Xavier Casadevall I Solvas, Marianne S. Carlon

“…We also detail procedures for applying pre-trained models and training custom models on new customized datasets.For complete details on the use and execution of this protocol, refer to Bulcaen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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GNI Corpus Version 1.0: Annotated Full-Text Corpus of to Support Biomedical Information Extraction by So-Yeon Oh, Ji-Hyeon Kim, Seo-Jin Kim, Hee-Jo Nam, Hyun-Seok Park

“…Text corpus for this journal annotated with various levels of linguistic information would be a valuable resource as the process of information extraction requires syntactic, semantic, and higher levels of natural language processing. In this study, we publish our new corpus called GNI Corpus version 1.0, extracted and annotated from full texts of Genomics & Informatics, with NLTK (Natural Language ToolKit)-based text mining script. …”
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Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology by Vineeth A. Raveendran, Jessica C. Pressey, Melanie A. Woodin

“…We describe steps for culturing neurons isolated from postnatal pups and electrophysiological measurement of EGABA.For complete details on the use and execution of this protocol, please refer to Raveendran et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Contributors to the Edition by Prof Ronit Frenkel

“…The Thinker is a ‘hybrid’ journal, publishing both journalistic pieces with more academic articles and contributors can now opt to have their submissions peer reviewed. …”
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Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells by John Kim, Hilla Weidberg

“…We then show how co-immunoprecipitation can be used to test the stalling of precursors inside TOM.For complete details on the use and execution of this protocol, please refer to Kim et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software by Pieter Both, Soochi Kim, Jengmin Kang, Marina Arjona, Daniel I. Benjamin, Christopher W. Nutter, Armon Goshayeshi, Thomas A. Rando

“…By automating these traditionally labor-intensive processes, Myotally improves research efficiency and data consistency. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol to generate a 3D atherogenesis-on-chip model for studying endothelial-macrophage crosstalk in atherogenesis by Katie M.L. Hanford, Kim E. Dzobo, Miranda Versloot, Jorge Peter, Jeffrey Kroon

“…This protocol could be adapted toward more complex plaque microenvironment or other disease settings. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Sensor Resource Sharing Approaches in Sensor-Cloud Infrastructure by Yujin Lim, Jaesung Park

“…In this paper, we introduce sensor-cloud infrastructure and present not only comprehensive technical issues like sensor description and publish/subscribe middleware but also applications using sensor-cloud infrastructure. …”
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Protocol for fecal microbiota transplantation: A microaerophilic approach for mice housed in a specific pathogen-free facility by Shari Wouters, Hugo Moors, Mieke Verslegers, Natalie Leys, Surbhi Malhotra-Kumar, Samir Kumar-Singh, Mohamed Mysara

“…This protocol focuses on age- and gender-matched, healthy donor mice using a mobile and cost-effective alternative to an anoxic cabinet. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics by Yan Ma, Keshu Dong, Jie Hu, Yiyun Tang, Hanfu Xu

“…This protocol enables the exploration of the spatial distribution of SG cells at single-cell resolution.For complete details on the use and execution of this protocol, please refer to Ma et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

“…By preserving tumor architecture and heterogeneity, this model is applicable for evaluating tumor characteristics, immune interactions, and treatment efficacy in translational cancer research. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

“…We detail the steps for using whole-cell patch-clamp electrophysiology to investigate the properties of synaptic transmission and synaptic plasticity in this model system.For complete details on the use and execution of this protocol, please refer to Patton et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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An architecture of database-based open resource service networking by Fenlin ZHOU, Xiaocong QIAN, Dongyu YUAN

“…The deep cloud-network integration hopes that the network side can provide fine-grained and on-demand customized services for cloud-side services, which poses a great challenge to existing IP bearer networks.To this end, an architecture of database-based open resource service networking was proposed.By abstracting the atomic service capabilities of the network and publishing services through a strongly consistent distributed key-value database, on-demand use of networking services at the cloud-side, joint cloud-network orchestration, and one point of business access could be achieved, which could flexibly meet the needs of cloud-network integration and achieve efficient carrying and refined services for diversified businesses.…”
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Contributors to this edition by Ronit Frenkel

“…The Thinker is a ‘hybrid’ journal, publishing both journalistic pieces with more academic articles and contributors can now opt to have their submissions peer reviewed. …”
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Contributors to this edition by Ronit Frenkel

“…The Thinker is a ‘hybrid’ journal, publishing both journalistic pieces with more academic articles and contributors can now opt to have their submissions peer reviewed. …”
Get full text

Occidente, canon y literatura hispanoamericana by Dante Liano

“…This article proposes the following idea: that, with what is known as the «boom», the Spanish American literature entirely joined modernity (at least, in the case of modern publishing industry’s devices) and also became a member of western canon. …”
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