PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages by Sreelakshmi K. Sreenivasamurthy, Carlos Gustavo Baptista, Christopher M. West, Ira J. Blader, Jeffrey D. Dvorin

“…PfFBXO1-deficient gametocytes are abnormally shaped and fail to fully mature. Proteomic analysis identified PfSKP1 as one of PfBXO1’s stable interacting partners, while other major proteins included multiple IMC pellicle and membrane proteins. …”
Get full text

Methyl-Arginine Profile of Brain from Aged PINK1-KO+A53T-SNCA Mice Suggests Altered Mitochondrial Biogenesis by Georg Auburger, Suzana Gispert, Nadine Brehm

“…Now we studied brain hemispheres of DM mice at age of 18 months in a hypothesis-free approach, employing a quantitative label-free global proteomic mass spectrometry scan of posttranslational modifications focusing on methyl-arginine. …”
Get full text

Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation by Imelda Ontoria-Oviedo, Itziar Palacios, Joaquín Panadero, Belén Sánchez, Francisco García-García, Adolfo López-Cerdán, Akaitz Dorronsoro, Delia Castellano, Luis Rodríguez-Borlado, Antonio Bernad, Pilar Sepúlveda

“…The CPC and BM-MSC receptome were analyzed by proteomic analysis of biotin-labeled surface proteins. …”
Get full text

The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon by Lu Zou, Dan Cao, Qing Sun, Wenjun Yu, Bingzong Li, Guoqiang Xu, Liang Zhou

“…In this study, we explored the interactome for CRBN using proximity labeling technique TurboID and quantitative proteomics, and then investigated the antileukemia effect of Len. …”
Get full text

Reprogramming of iPSCs to NPCEC-like cells by biomimetic scaffolds for zonular fiber reconstruction by Tianhui Chen, Zhongxing Chen, Juan Du, Min Zhang, Zexu Chen, Qingyi Gao, Aodong Chen, Qinghao Meng, Yang Sun, Yan liu, Linghao Song, Xinyue Wang, Pranav Prakash Edavi, Chen Xu, Hongmei Zhang, Jinhai Huang, Yongxiang Jiang

“…Ocular tissue and aqueous humour samples from patients with EL were subjected to RNA sequencing and Olink high-throughput proteomic analysis, revealing the downregulation of pathogenic genes (FBN1, MFAP2) and upregulation of secretory proteins (IL-12, MMP-1). …”
Get full text

Bilayer 3D co‐culture platform inducing the differentiation of normal fibroblasts into cancer‐associated fibroblast like cells: New in vitro source to obtain cancer‐associated fibr... by Yeon Ju Kim, Hyeon Song Lee, Dohyun Kim, Hwa Kyung Byun, Woong Sub Koom, Won‐Gun Koh

“…The mRNA expression levels of CAF‐related markers were significantly upregulated in 3D, but not in 2D co‐culture. Proteomic analysis identified upregulated proteins associated with CAFs, further confirming the transformation of normal fibroblasts into CAF within the proposed 3D co‐culture platform. …”
Get full text

RSK4 promotes the metastasis of clear cell renal cell carcinoma by activating RUNX1-mediated angiogenesis by Jing Ma, Yanru Yang, Kaijing Wang, Jin Liu, Junyi Feng, Gongcheng Wang, Shuangping Guo, Linni Fan

“…We analyzed changes in the expression of target genes through transcriptomic and proteomic assessments. We also conducted tube formation assays and VEGF ELISAs to understand the role of RSK4 in angiogenesis. …”
Get full text

The metabolomic approach to the assessment of cultivar specificity of Brassica napus L. seeds by G. N. Smolikova, A. L. Shavarda, I. V. Alekseichuk, V. V. Chantseva, S. S. Medvedev

“…Recent biomolecular studies tend to involve combinations of different methods and approaches that allow analyzing organisms on the genomic and proteomic levels, as well as on the level of metabolomics. …”
Get full text

CryoSCAPE: Scalable immune profiling using cryopreserved whole blood for multi-omic single cell and functional assays by Alexander T. Heubeck, Cole Phalen, Neel Kaul, Peter J. Wittig, Jessica Garber, Morgan Weiss, Palak C. Genge, Zachary Thomson, Claire Gustafson, Julian Reading, Peter J. Skene

“…We present CryoSCAPE (Cryopreservation for Scalable Cellular And Proteomic Exploration), a scalable method for immune studies of human PBMC with multi-omic single cell assays using direct cryopreservation of whole blood. …”
Get full text

Starvation-induced metabolic rewiring affects mTORC1 composition in vivo by Edgar Kaade, Simone Mausbach, Nina Erps, Marc Sylvester, Farhad Shakeri, Ron D. Jachimowicz, Volkmar Gieselmann, Melanie Thelen

“…Therefore, we investigated the changes of the proteome of liver lysosomes in mice starved short-term for 6h or long-term for 24h. …”
Get full text

Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice... by Wenqi Wang, Yidi Qu, Siyu Li, Jinyu Chu, Hongxin Yang, Lirong Teng, Di Wang

“…Based on the screening of proteomic analysis on hippocampus, CCG were confirmed that it could regulate the expression levels of proteins related to mitochondrial dysfunction, mainly through activating on AMPK/Nrf2 signaling, in APP/PS1 mice and L-Glu-exposed HT22 cells. …”
Get full text

The triterpenoids-enriched extracts from Antrodia cinnamomea mycelia attenuate alcohol-induced chronic liver injury via suppression lipid accumulation in C57BL/6 mice by Yange Liu, Ronglong Chen, Lanzhou Li, Ruitao Dong, Hui Yin, Yawen Wang, Anhui Yang, Jianbin Wang, Changtian Li, Di Wang

“…Combining with the pathological observations, ACT displayed an anti-steatosis effects to restrain the progress of ALD. Based on proteomic analysis and enzyme-linked immunosorbent assay, ACT had been confirmed to regulate the levels of lipid biogeneration-related factors and depressed the over-accumulation of hepatic reactive oxygen species. …”
Get full text

Modulation of biological activities in adipose derived stem cells by histone deacetylation by Sallam Abdallah, Mouna Tabebi, Sawsan Qanadilo, Neserin Ali, Jing Wang, Pádraig D’Arcy, Wen Zhong, Folke Sjoberg, Moustafa Elmasry, Ahmed El-Serafi

“…Microarray analysis revealed 74 upregulated and 40 downregulated differentially expressed genes, including upregulation of P53 targets, CDKN1A and MDM2. Proteomic analysis identified 631 upregulated and 823 downregulated proteins compared to the vehicle. …”
Get full text

Comprehensive Enteroviral Serology Links Infection and Anti‐Melanoma Differentiation‐Associated Protein 5 Dermatomyositis by Sahana Jayaraman, Eleni Tiniakou, William R. Morgenlander, Miso Na, Lisa Christopher‐Stine, H. Benjamin Larman

“…Methods We employed Phage ImmunoPrecipitation Sequencing to profile serum antibodies against the human proteome, the human virome, and a comprehensive enterovirus library. …”
Get full text

Spatially Resolved Multiomics: Data Analysis from Monoomics to Multiomics by Changxiang Huan, Jinze Li, Yingxue Li, Shasha Zhao, Qi Yang, Zhiqi Zhang, Chuanyu Li, Shuli Li, Zhen Guo, Jia Yao, Wei Zhang, Lianqun Zhou

“…Spatial monoomics techniques in epigenomics, genomics, transcriptomics, proteomics, and metabolomics can enhance our understanding of biological functions and cellular identities by simultaneously measuring tissue structures and biomolecule levels. …”
Get full text

Associations between accurate measures of adiposity and fitness, blood proteins, and insulin sensitivity among South Asians and Europeans by Pik Fang Kho, Pik Fang Kho, Laurel Stell, Shirin Jimenez, Shirin Jimenez, Daniela Zanetti, Daniela Zanetti, Daniela Zanetti, Daniel J. Panyard, Kathleen L. Watson, Ashish Sarraju, Ashish Sarraju, Ming-Li Chen, Ming-Li Chen, Lars Lind, John R. Petrie, Khin N. Chan, Holly Fonda, Kyla Kent, Kyla Kent, Jonathan N. Myers, Jonathan N. Myers, Jonathan N. Myers, Latha Palaniappan, Latha Palaniappan, Latha Palaniappan, Fahim Abbasi, Themistocles L. Assimes, Themistocles L. Assimes, Themistocles L. Assimes, Themistocles L. Assimes

“…We explored this possibility by investigating the relationship between ‘gold standard’ measures of adiposity, fitness, selected proteomic biomarkers, and insulin sensitivity among a cohort of SAs and Europeans (EURs).MethodsA total of 46 SAs and 41 EURs completed ‘conventional’ (lifestyle questionnaires, standard physical exam) as well as ‘gold standard’ (dual energy X-ray absorptiometry scan, cardiopulmonary exercise test, and insulin suppression test) assessments of adiposity, fitness, and insulin sensitivity. …”
Get full text

Platelet‐derived apoptotic vesicles ameliorate nonalcoholic fatty liver disease by regulating lipid metabolism via apolipoprotein A‐II by Yuhe Jiang, Yike Liao, Zeying Wang, Lei Zhu, Yunsong Liu, Ping Zhang, Yuan Zhu, Wenyue Li, Yongsheng Zhou, Xiao Zhang

“…The results showed that PLT‐apoVs exhibited superior effects in diminishing lipid accumulation in liver induced by high‐fat diet than MSC‐apoVs. Through proteomic analysis, we defined and validated apolipoprotein A‐II (APOA2) as a regulator for apoV‐mediated MSC adipogenesis, which could be used as a target to enhance apoV therapeutic potential in the lipid metabolism biomedical field. …”
Get full text

NERVE 2.0: boosting the new enhanced reverse vaccinology environment via artificial intelligence and a user-friendly web interface by Andrea Conte, Nicola Gulmini, Francesco Costa, Matteo Cartura, Felix Bröhl, Francesco Patanè, Francesco Filippini

“…Abstract Background Vaccines development in this millennium started by the milestone work on Neisseria meningitidis B, reporting the invention of Reverse Vaccinology (RV), which allows to identify vaccine candidates (VCs) by screening bacterial pathogens genome or proteome through computational analyses. When NERVE (New Enhanced RV Environment), the first RV software integrating tools to perform the selection of VCs, was released, it prompted further development in the field. …”
Get full text

Ginsenoside F1 administration promotes UCP1-dependent fat browning and ameliorates obesity-associated insulin resistance by Yuhan Meng, Weili Li, Chenxing Hu, Si Chen, Haiyang Li, Feifei Bai, Lujuan Zheng, Ye Yuan, Yuying Fan, Yifa Zhou

“…We also investigated the ability of F1 on energy metabolism in obesity-induced diabetic mice, including body weight, body composition and energy expenditure. The results of proteomics showed that F1 significantly up-regulated thermogenesis proteins and lipolytic proteins, but down-regulated fatty acid synthesis proteins. …”
Get full text

A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells by Anna Babin-Ebell Gonçalves, Yifang Mao, Tinja Baljkas, Felix Wiedmann, Larissa Eis, Franziska Pilz, Manuel Winkler, Sina W. Kürschner-Zacharias, Marlene Hoffarth, Charlotta Funaya, Réza Shahidi, Cyrill Géraud, Chi-Chung Wu, Constanze Schmidt, Sergij Goerdt, Philipp-Sebastian Reiners-Koch, Mahak Singhal

“…Combining magnetic and fluorescence-activated cell sorting (FACS), LSECs from different zones of the hepatic sinusoid can now be isolated in high numbers in less than two hours for downstream applications including proteomics. Our protocol enables the isolation of LSECs from fibrotic liver tissues from mice and healthy liver tissues from higher vertebrate species (pigs), where traditional perfusion-based digestion protocols have very limited application. …”
Get full text