Elucidation of peptide screen for targeted identification of Yersinia pestis by nano-liquid chromatography tandem mass spectrometry by Priya Rani, Syed Imteyaz Alam, Sandeep Singh, Subodh Kumar

“…We employed a shotgun proteomic approach using nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) to discover Y. pestis-specific peptides. …”
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The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis by Louise Tzung-Harn Hsieh, Belinda S Hall, Jane Newcombe, Tom A Mendum, Sonia Santana Varela, Yagnesh Umrania, Michael J Deery, Wei Q Shi, Josué Diaz-Delgado, Francisco J Salguero, Rachel E Simmonds

“…Unbiased quantitative proteomics identified a profound effect on proteoglycans, driven by rapid loss of type II transmembrane proteins of the Golgi, including enzymes required for glycosaminoglycan (GAG) synthesis, combined with a reduction in the core proteins themselves. …”
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Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime by Debjit Roy, Xavier Michalet, Evan W. Miller, Kiran Bharadwaj, Shimon Weiss

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Changes of plasma cytokines content in Graves’ orbitopathy patients, could be potential markers by Ying Fu, Yuanyuan Zhang, Yaxin An, Bin Cao, Dong Zhao

“…We used label-free liquid chromatography–mass spectrometry/MS (LC–MS/MS) quantitative proteomic to analyze plasma cytokines content of Graves’ disease and GO patients. …”
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Involvement of Cholesterol Metabolic Pathways in Recovery from Noise-Induced Hearing Loss by Na Sai, Xi Shi, Yan Zhang, Qing-qing Jiang, Fei Ji, Shuo-long Yuan, Wei Sun, Wei-Wei Guo, Shi-Ming Yang, Wei-Ju Han

“…This is the first study to use proteomics analysis to analyze the molecular mechanisms of acute noise-induced hearing loss and its recovery in a large animal model (miniature pigs). …”
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BIOINFORMATIC ANALYSIS OF THE GENOME OF THE Geobacillus stearothermophilus 22 STRAIN ISOLATED FROM THE GARGA HOT SPRING, BAIKAL REGION by A. S. Rozanov, T. V. Ivanisenko, A. V. Bryanskaya, S. V. Shekhovtsov, M. D. Logacheva, O. V. Saik, T. K. Malup, P. S. Demenkov, T. N. Goryachkovskaya, V. A. Ivanisenko, S. E. Peltek

“…Nucleotide sequences (contigs) of the strain were found to be similar to the genome of the thermophilic strain G. kaustophilus Y412MC52. The proteome of the new strain was analyzed. Fragments associated with hemicellulases (endoxylanase, beta xylosidase, and arabinofuranosidase) and the endoxylanase enzyme were detected.…”
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High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human... by Johanna L. Grün, Aaron N. Manjarrez-Reyna, Angélica Y. Gómez-Arauz, Sonia Leon-Cabrera, Felix Rückert, José M. Fragoso, Nallely Bueno-Hernández, Sergio Islas-Andrade, Guillermo Meléndez-Mier, Galileo Escobedo

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Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies by Wen-Ting K. Tsai, Yinyin Li, Zhaojun Yin, Peter Tran, Qui Phung, Zhenru Zhou, Kun Peng, Dan Qin, Sien Tam, Christoph Spiess, Jochen Brumm, Manda Wong, Zhengmao Ye, Patrick Wu, Sivan Cohen, Paul J. Carter

“…Assay methods used include dendritic cell internalization, T cell proliferation, and T cell epitope identification by in silico prediction and MHC-associated peptide proteomics. Data from each method were considered independently and then together for an overall integrated immunogenicity risk assessment. …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

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Discovery of a sushi domain-containing protein 2-positive phenotype in circulating tumor cells of metastatic breast cancer patients by Kai Bartkowiak, Parinaz Mossahebi Mohammadi, Paula Nissen, Stefan Werner, David Agorku, Antje Andreas, Maria Geffken, Sven Peine, Karl Verpoort, Thomas M. Deutsch, Laura L. Michel, Andreas Schneeweiss, Verena Thewes, Andreas Trumpp, Olaf Hardt, Volkmar Müller, Sabine Riethdorf, Hartmut Schlüter, Klaus Pantel

“…After a LC-MC/MS based proteomics analysis of CTC-ITB-01, we found extraordinary high levels of the poorly characterized protein SUSD2 (sushi domain-containing protein 2) in CTC-ITB-01. …”
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Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy by Sarala Raj Murthi, Andreas Petry, Bachuki Shashikadze, Jan B. Stöckl, Manuel Schmid, Gianluca Santamaria, Karin Klingel, Damir Kračun, Xinpei Chen, Sabine Bauer, Joachim P. Schmitt, Florian Flenkenthaler, Josh Gorham, Christopher N. Toepfer, David Potěšil, Pavel Hruška, Zbyněk Zdráhal, Zsuzsanna Mayer, Mathieu Klop, Luisa Lehmann, Yishi Qin, Laura Papanakli, Nadine Spielmann, Alessandra Moretti, Thomas Fröhlich, Peter Ewert, Stefan Holdenrieder, Jonathan G. Seidman, Christine E. Seidman, Agnes Görlach, Cordula M. Wolf

“…Cardiomyocyte-specific abolishment of Hif-1a blunted the disease phenotype, as evidenced by decreased left ventricular wall thickness, reduced myocardial fibrosis, disordered SRX/DRX state and ROS production. cHif1aKO induced normalization of pro-hypertrophic and pro-fibrotic left ventricular remodeling signaling evidenced on whole transcriptome and proteomics analysis in α-MHC719/+ mice. Proteomics of serum samples from patients with early onset HCM revealed significant modulation of HIF. …”
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Inflammation-Related Mechanisms in Chronic Kidney Disease Prediction, Progression, and Outcome by Simona Mihai, Elena Codrici, Ionela Daniela Popescu, Ana-Maria Enciu, Lucian Albulescu, Laura Georgiana Necula, Cristina Mambet, Gabriela Anton, Cristiana Tanase

“…Recent omics advances could provide novel insights in deciphering the disease pathophysiology; thus, identification of circulating biomarker panels using state-of-the-art proteomic technologies could improve CKD early diagnosis, monitoring, and prognostics. …”
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Impact of High Glucose on Bone Collagenous Matrix Composition, Structure, and Organization: An Integrative Analysis Using an Ex Vivo Model by Rita Araújo, Ricardo N. M. J. Páscoa, Raquel Bernardino, Pedro S. Gomes

“…A comprehensive approach integrating histological evaluation, histomorphometry, ATR-FTIR spectroscopy, and proteomics was adopted to unravel structural, biochemical, and molecular changes in the ECM. …”
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Therapeutic effects of platelet-derived extracellular vesicles on viral myocarditis correlate with biomolecular content by Danielle J. Beetler, Danielle J. Beetler, Danielle J. Beetler, Presley Giresi, Damian N. Di Florio, Damian N. Di Florio, Damian N. Di Florio, Jessica J. Fliess, Elizabeth J. McCabe, Molly M. Watkins, Molly M. Watkins, Molly M. Watkins, Vivian Xu, Matthew E. Auda, Katelyn A. Bruno, Katelyn A. Bruno, Emily R. Whelan, Emily R. Whelan, Emily R. Whelan, Stephen P. C. Kocsis, Brandy H. Edenfield, Sierra A. Walker, Sierra A. Walker, Sierra A. Walker, Logan P. Macomb, Kevin C. Keegan, Angita Jain, Angita Jain, Angita Jain, Andrea C. Morales-Lara, Isha Chekuri, Anneliese R. Hill, Houssam Farres, Joy Wolfram, Joy Wolfram, Atta Behfar, Atta Behfar, Paul G. Stalboerger, Andre Terzic, Andre Terzic, Leslie T. Cooper, DeLisa Fairweather, DeLisa Fairweather, DeLisa Fairweather

“…Proteome and miR sequencing of EVs revealed that PEV miRs specifically targeted antiviral, Toll-like receptor (TLR)4, and inflammasome pathways known to contribute to myocarditis while pmPEV contained general immunoregulatory miRs.DiscussionThese differences in EV content corresponded to the differing anti-inflammatory effects of the two types of EVs on viral myocarditis.…”
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Formation mechanisms of ethyl acetate and organic acids in Kluyveromyces marxianus L1-1 in Chinese acid rice soup by Na Liu, Likang Qin, Laili Hu, Song Miao

“…This study aims to explore the formation mechanism of ethyl acetate and organic acids in acid rice soup (rice-acid soup) inoculated with Kluyveromyces marxianus L1-1 through the complementary analysis of transcriptome and proteome. The quantity of K. marxianus L1-1 varied significantly in the fermentation process of rice-acid soup and the first and third days were the two key turning points in the growth phase of K. marxianus L1-1. …”
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An Interventional Magnetic Resonance Imaging Technique for the Molecular Characterization of Intraprostatic Dynamic Contrast Enhancement by Cynthia Ménard, Robert C. Susil, Peter Choyke, Jonathan Coleman, Robert Grubb, Ahmed Gharib, Axel Krieger, Peter Guion, David Thomasson, Karen Ullman, Sandeep Gupta, Virginia Espina, Lance Liotta, Emanuel Petricoin, Gabor Fitchtinger, Louis L. Whitcomb, Ergin Atalar, C. Norman Coleman, Kevin Camphausen

“…Here we demonstrate the feasibility of precisely colocalizing DCE-MRI data with the genomic and proteomic profiles of underlying biopsy tissue using a novel MRI-guided biopsy technique in patients with prostate cancer.…”
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Functional heterogeneity of mesenchymal stem cells and their therapeutic potential in the K18-hACE2 mouse model of SARS-CoV-2 infection by Kátia Nunes da Silva, Fernanda Martins Marim, Gisele Vieira Rocha, Zaquer Suzana Munhoz Costa-Ferro, Luciana Souza de Aragão França, Carolina Kymie Vasques Nonaka, Bruno Diaz Paredes, Erik Aranha Rossi, Erick Correia Loiola, Corynne Stephanie Ahouefa Adanho, Rachel Santana Cunha, Mayck Medeiros Amaral da Silva, Fernanda Ferreira Cruz, Vivian Vasconcelos Costa, Dalila Lucíola Zanette, Clarissa Araújo Gurgel Rocha, Renato Santana Aguiar, Patricia Rieken Macedo Rocco, Bruno Solano de Freitas Souza

“…The cells were assessed for their proliferative capacity, immunophenotype, trilineage differentiation, proteomic profile, and in vitro immunomodulatory potential by co-culture with activated lymphocytes. …”
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Sex differences in epigenetic ageing for older people living with HIVResearch in context by Carrie D. Johnston, Alina P.S. Pang, Eugenia L. Siegler, Charlene Thomas, Chelsie O. Burchett, Mia Crowley, Rochelle O'Brien, Lishomwa C. Ndhlovu, Marshall J. Glesby, Michael J. Corley

“…We profiled plasma biomarkers with Olink high-throughput proteomics to test associations with epigenetic age acceleration. …”
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Propionate metabolism in Desulfurella acetivorans by Eugenio Pettinato, Thomas M. Steiner, Eric A. Cassens, Thomas Geisberger, Christian Seitz, Simone König, Wolfgang Eisenreich, Ivan A. Berg

“…However, activities of the methylcitrate cycle enzymes could not be detected in extracts of propionate-grown cells, and experiments with [U-13C3]propionate and comparative proteomic analysis of acetate- and propionate-grown cells suggested that the methylcitrate cycle is not active during propionate growth. …”
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Usefulness of C-Reactive Protein as a Marker for Prediction of Future Coronary Events in the Asian Indian Population: Indian Atherosclerosis Research Study by Veena S. Rao, Natesha B. Kadarinarasimhiah, Shibu John, Sridhara Hebbagodi, Jayashree Shanker, Vijay V. Kakkar

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