Protocol for in vitro phase separation of N terminus of CIDEC proteins by Yiming Lu, Zanzan Zhu, Jianqin Wang, Xuchao Lyu, Peng Li, Feng-Jung Chen

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Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis by Seiryo Ogata, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki

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Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes by Jinyu Yang, Runying Wang, Na Ren, De-gang Zhao, Xiaozhen Huang, Yichen Zhao

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A novel plasmid‐based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells by Geyao Dong, Tsuyoshi Nakai, Tetsuo Matsuzaki

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Melatonin protects rats against bisphenol A-induced testicular dysfunction through the upregulation of α-smooth muscle actin, vimentin, and S-100 proteins by Olumide Samuel Ajani, Samuel Gbadebo Olukole, Matthew Olugbenga Oyeyemi, Ekundayo Stephen Samuel

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Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli by Sven O. Dahms, Alexander C. Wieland, Hans Brandstetter, Elfriede Dall

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Protocol for purifying and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes by Felichi Mae Arines, Aleksander Wielenga, Randy B. Stockbridge, Ming Li

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Expression of a Novel HIV-1 Gag-Pol-Env-Nef-Rev Multi-Epitope Construct in Escherichia coli by Elahe Akbari, Soheila Ajdari, Esmat Mirabzadeh Ardakani, Elnaz Agi, Vahid Khalaj, Azam Bolhassani

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Differential expression of plasma proteins and pathway enrichments in pediatric diabetic ketoacidosis by Paolo Spagnolo, Enis Cela, Maitray A. Patel, David Tweddell, Mark Daley, Cheril Clarson, Saverio Stranges, Gediminas Cepinskas, Douglas D. Fraser

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Protocol for measuring β-arrestin1-phosphorylated peptide interaction and analyzing conformational dynamics of β-arrestin1 by Kiae Kim, Ka Young Chung

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Precision in 3D: A Fast and Accurate Algorithm for Reproducible Motoneuron Structure and Protein Expression Analysis by Morgan Highlander, Shelby Ward, Bradley LeHoty, Teresa Garrett, Sherif Elbasiouny

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Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli by Christoph Holzner, Katharina Böttinger, Christian G. Huber, Elfriede Dall, Hans Brandstetter

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Restoration of Autophagy and Apoptosis in Myelodysplastic Syndromes: The Effect of Azacitidine in Disease Pathogenesis by Georgia Tsekoura, Andreas Agathangelidis, Christina-Nefeli Kontandreopoulou, Eirini Sofia Fasouli, Eleni Katsantoni, Vaia Pliaka, Leonidas Alexopoulos, Eleni Katana, Myrto Papaioannou, Georgia Taktikou, Maria Eleftheria Strataki, Angeliki Taliouraki, Marina Mantzourani, Nora-Athina Viniou, Panagiotis T. Diamantopoulos, Panagoula Kollia

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Protocols for monitoring condensate formation and dynamics between the phase-separating proteins SET/TAF-Iβ and cytochrome c by Miguel Á. Casado-Combreras, Miguel A. De la Rosa, Irene Díaz-Moreno

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A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems by Agnieszka M. Murakami, Katsuhiro Nagatomo, Hiroshi Koda, Yasutaka Niwa, Manabu Murakami

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Protein Expression Platforms and the Challenges of Viral Antigen Production by Jamie R. V. Sookhoo, Zachary Schiffman, Aruna Ambagala, Darwyn Kobasa, Keith Pardee, Shawn Babiuk

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Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases by Christian Gusenda, Svenja Berlage, Ines Burkhart, Benjamin Chagot, Kira J. Weissman, Harald Schwalbe, Martin Grininger

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Effects of tryptophan supplementation on performance, intestinal morphology and protein abundance of tight junction protein and indoleamine 2, 3-dioxygenase in weaned pigs by Pei Mao, Naizhi Hu, Mengmeng Gao, Lingping Zhao, Chengsheng Song, Wenfeng Ma

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Quantitative measurement of allele‐specific protein expression in a diploid yeast hybrid by LC‐MS by Zia Khan, Joshua S Bloom, Sasan Amini, Mona Singh, David H Perlman, Amy A Caudy, Leonid Kruglyak

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A global view of protein expression in human cells, tissues, and organs by Fredrik Pontén, Marcus Gry, Linn Fagerberg, Emma Lundberg, Anna Asplund, Lisa Berglund, Per Oksvold, Erik Björling, Sophia Hober, Caroline Kampf, Sanjay Navani, Peter Nilsson, Jenny Ottosson, Anja Persson, Henrik Wernérus, Kenneth Wester, Mathias Uhlén

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