Optimizing longifolene production in Yarrowia lipolytica via metabolic and protein engineering by Yi-Tong Yao, Xiao Zhang, Chen-Yu Wang, Yu-He Zhang, Da-Wei Li, Wei-Dong Yang, Hong-Ye Li, Li-Gong Zou

Subjects: Get full text

Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering by William S. Grubbe, Bixia Zhang, Aileen Kauffman, Fabian Byléhn, Kasia Padoł, Hae-Gwang Jung, Seung Bum Park, Jessica M. Priest, Engin Özkan, Juan J. de Pablo, T. Jake Liang, Minglei Zhao, Juan L. Mendoza

Get full text

Structure‐based computational design of antibody mimetics: challenges and perspectives by Elton J. F. Chaves, Danilo F. Coêlho, Carlos H. B. Cruz, Emerson G. Moreira, Júlio C. M. Simões, Manassés J. Nascimento‐Filho, Roberto D. Lins

Subjects: Get full text

A novel conditional active biologic anti-EpCAM x anti-CD3 bispecific antibody with synergistic tumor selectivity for cancer immunotherapy by Gerhard Frey, Ana Paula G. Cugnetti, Haizhen Liu, Charles Xing, Christina Wheeler, Hwai Wen Chang, William J. Boyle, Jay M. Short

Subjects: Get full text

Optimizing the thermostability of triketone dioxygenase for engineering tolerance to mesotrione herbicide in soybean and cotton by Stephen M. G. Duff, Stephen M. G. Duff, Lei Shi, Danqi Chen, Xiaoran Fu, Mingsheng Peng, Clayton T. Larue, Janice Weihe, Jessica Koczan, Brian Krebel, Qungang Qi

Subjects: “…protein engineering…”
Get full text

Engineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET by Andreas Evers, Simon Krah, Deniz Demir, Ramona Gaa, Desislava Elter, Christian Schroeter, Stefan Zielonka, Nicolas Rasche, Julia Dotterweich, Christine Knuehl, Achim Doerner

“…Structural molecular modeling of paratope combinations for preferential inter-target binding combined with protein engineering for manufacturability yielded deep insights into the capabilities of rational and library approaches. …”
Get full text

Simultaneous exposure to microplastics and heavy metal lead induces oxidative stress, histopathological damage, and immune dysfunction in marine mussel Mytilus coruscus by Chuanyue Chen, Xueer Wang, Yingrong Jin, Yinchang Yao, Suchang Li, Zhi Liao, Xiaolin Zhang, Xiaojun Yan

Get full text

Impact of antibody architecture and paratope valency on effector functions of bispecific NKp30 x EGFR natural killer cell engagers by Ammelie Svea Boje, Lukas Pekar, Katharina Koep, Britta Lipinski, Brian Rabinovich, Andreas Evers, Carina Lynn Gehlert, Steffen Krohn, Yanping Xiao, Simon Krah, Rinat Zaynagetdinov, Lars Toleikis, Sven Poetzsch, Matthias Peipp, Stefan Zielonka, Katja Klausz

Get full text

Improving the N-glycosylation occupancy of plant-produced IgG1 by engineering the amino acid environment at Asn297 by Kathrin Göritzer, Valentina Ruocco, Ulrike Vavra, Shiva Izadi, Omayra C. Bolaños-Martínez, Thareeya Phetphoung, Nuttapat Pisuttinusart, Waranyoo Phoolcharoen, Richard Strasser

“…This study addressed this limitation of the widely used plant-based expression platform Nicotiana benthamiana by employing protein engineering to enhance N-glycosylation occupancy in plant-produced IgG1. …”
Get full text

Persistent Topological Laplacians—A Survey by Xiaoqi Wei, Guo-Wei Wei

“…Persistent topological Laplacians have demonstrated superior performance over persistent homology in the analysis of large-scale protein engineering datasets. In this survey, we offer a pedagogical review of persistent topological Laplacians formulated in various mathematical settings, including simplicial complexes, path complexes, flag complexes, digraphs, hypergraphs, hyperdigraphs, cellular sheaves, and <i>N</i>-chain complexes.…”
Get full text

Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…Next, and prompted by recent developments in machine learning (ML)-guided protein engineering, we used an unbiased ML- and structure-guided approach to rapidly and efficiently generate a different variant with recovered affinity. …”
Get full text

Psychrophilic Enzymes: From Folding to Function and Biotechnology by Georges Feller

Get full text

Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL) by Marcus Wäneskog, Emma Elise Hoch-Schneider, Shilpa Garg, Christian Kronborg Cantalapiedra, Elena Schäfer, Michael Krogh Jensen, Emil Damgaard Jensen

“…ABSTRACT High-throughput DNA transformation techniques are invaluable when generating high-diversity mutant libraries, a cornerstone of successful protein engineering. However, transformation efficiencies have a direct correlation with the probability of introducing multiple DNA molecules into each cell, although reliable library screenings require cells that contain a single unique genotype. …”
Get full text

Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathways by Renee Hastings, Arjun K. Aditham, Nicole DelRosso, Peter H. Suzuki, Polly M. Fordyce

“…Abstract Understanding how proteins discriminate between preferred and non-preferred ligands (‘selectivity’) is essential for predicting biological function and a central goal of protein engineering efforts, yet the biophysical mechanisms underpinning selectivity remain poorly understood. …”
Get full text

Biocatalytic enantioselective formation and ring-opening of oxetanes by Xia Hua, Yuan-Fei Wang, Xiao Jin, Hong-Yin Yu, Hui-Hui Wang, Yong-Zheng Chen, Nan-Wei Wan

“…Herein, we report the discovery and subsequent protein engineering of a unique halohydrin dehalogenase to develop a biocatalytic platform for enantioselective formation and ring-opening of oxetanes. …”
Get full text

Active learning-assisted directed evolution by Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold

“…Overall, ALDE is a practical and broadly applicable strategy to unlock improved protein engineering outcomes.…”
Get full text

Discovery of potent allosteric antibodies inhibiting EGFR by Léxane Fournier, Lukas Pekar, Birgitta Leuthner, Harald Kolmar, Lars Toleikis, Stefan Becker

Get full text

Sensing and Degradation of Organophosphorus Compounds by Exploitation of Heat-Loving Enzymes by Giuseppe Manco, Eros A. Lampitella, Nagendra S. K. Achanta, Giuliana Catara, Maria Marone, Elena Porzio

“…Finally, we will make examples of integration between sensing and decontamination systems, including protein engineering to enhance OP-degrading activities and detailed characterization of the best variants.…”
Get full text

Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity by Evelyn F Okal, Philip A. Romero, Pete Heinzelman

“…This study leverages protein engineering to enhance ACE2’s therapeutic potential for treating conditions such as respiratory viral infections, acute respiratory distress syndrome, and diabetes. …”
Get full text

One-step introduction of the hexafluoroisobutyl group, synthesis of (S)-5,5,5,5',5',5'-hexafluoroleucine, and its incorporation into peptides and proteins by Delamare, Aline, Naulet, Guillaume, Mummadi, Sandeep, Guichard, Gilles, Compain, Guillaume

“…Overall, this work opens new perspectives in organofluorine chemistry, medicinal chemistry, and protein engineering, which are also discussed herein.…”
Get full text