Selective Serotonin Reuptake Inhibitors: Antimicrobial Activity Against ESKAPEE Bacteria and Mechanisms of Action by Thiago Hideo Endo, Mariana Homem de Mello Santos, Sara Scandorieiro, Bruna Carolina Gonçalves, Eliana Carolina Vespero, Márcia Regina Eches Perugini, Wander Rogério Pavanelli, Gerson Nakazato, Renata Katsuko Takayama Kobayashi

“…Regarding their mechanisms of action as antibacterials, for the first time, increased membrane permeability was detected, as evidenced by heightened dye absorption, along with the increased presence of total proteins and dsDNA in the extracellular medium of <i>Escherichia coli</i> ATCC2 25922 and <i>Staphylococcus aureus</i> ATCC 25923, and oxidative stress was also detected in bacteria treated with sertraline and paroxetine, with reduced efficiency observed in the presence of antioxidants and higher levels of oxygen-reactive species evidenced by their reaction with 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate. …”
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Enhanced detection of circulating tumor cells using a MUC1 promoter-driven recombinant adenovirus by Cheng Wang, Huihui Gu, Jia Cai, Chuandong Zhu, Qin Zheng, Hanfeng Xu, Lixue Wang, Yuan Wan

“…In this study, we judiciously constructed a recombinant MUC1-dependent adenovirus (rAdF35-MUC1) that can selectively replicate and overexpress copepod super green fluorescent proteins (copGFP) in MUC1-positive tumor cells to investigate its role in the detection of CTCs.MethodsWe conducted a comparative study between rAdF35-MUC1 and the existing hTERT-dependent adenovirus (rAdF35-hTERT). …”
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Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity by Can Can, Xinyu Yang, Hexiao Jia, Hanyang Wu, Xiaodong Guo, Yihong Wei, Ziting Jia, Wancheng Liu, Amin Zhang, Na He, Hailei Zhang, Daoxin Ma

“…RNA pull-down assay, mass spectrometry, immunofluorescence, co-immunoprecipitation and western blot were performed to identify and confirm the circ_0006896 interacting proteins. Results CircRNA expression patterns in exosomes differ significantly between AML and controls compared to lncRNAs or mRNAs. …”
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Phenolic and amino acid profiles, and antioxidant properties of third generation snack foods adding Citrus mitis by-products and milk powder: impact of extrusion processing and mic... by Xóchitl Ariadna Ruiz-Armenta, Carlos Iván Delgado-Nieblas, José de Jesús Zazueta-Morales, Ernesto Aguilar-Palazuelos, Perla Rosa Fitch-Vargas, José Alberto Gallegos-Infante, José Ángel López-Valenzuela, María Fernanda Quintero-Soto

“…The microwave-expanded snack foods had acceptable sensory properties evaluated by consumers, and adding raw materials rich in phytochemical compounds and high-quality proteins enhanced their nutraceutical/nutritional properties.…”
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Overexpression of IL-6 and STAT3 may provide new insights into ovine pulmonary adenocarcinoma development by Corina Toma, Roxana Popa, Lidia Ciobanu, Ioana Baldea, Irina Amorim, Diana Bochynska, Alan Wolfe, Andrada Negoescu, Claudiu Gal, Marian Taulescu

“…Using WB analyses, a highly significant amount of both IL-6 (p = 0.0078) and STAT3 (p < 0.0001) proteins were present in lung neoplasms, by comparison to the control lungs. …”
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Evaluation of tick salivary and midgut extracellular vesicles as anti-tick vaccines in White-tailed deer (Odocoileus virginianus) by Julia Gonzalez, Cristina Harvey, Cárita de Souza Ribeiro-Silva, Brenda Leal-Galvan, Kelly A. Persinger, Pia U. Olafson, Tammi L. Johnson, Adela Oliva Chavez

“…These results indicate that proteins within female tick SG and MG vesicles are not good candidates for vaccine design against nymphs; however, the on-host adult mortality suggests that tick EVs harbor protective antigens against A. americanum females.…”
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Altered Toll-Like Receptor Signalling in Children with Down Syndrome by Dean Huggard, W. J. Koay, Lynne Kelly, Fiona McGrane, Emer Ryan, Niamh Lagan, Edna Roche, Joanne Balfe, T. Ronan Leahy, Orla Franklin, Ana Moreno-Oliveira, Ashanty M. Melo, Derek G. Doherty, Eleanor J. Molloy

“…We evaluated TLR pathways in 3 ways: determining the expression of TLR2 on the surface of neutrophils and monocytes by flow cytometry, examining the gene expression of key regulatory proteins involved in TLR signal propagation, MyD88, IRAK4, and TRIF, by quantitative PCR, and lastly determining the cytokine production by ELISA following immunomodulation with proinflammatory stimuli (lipopolysaccharide (LPS), Pam3Csk4) and the anti-inflammatory agent SsnB. …”
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The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae by Chongyang Wu, Chaoqin Lin, Xinyi Zhu, Hongmao Liu, Wangxiao Zhou, Junwan Lu, Licheng Zhu, Qiyu Bao, Cong Cheng, Yunliang Hu

“…The extended-spectrum β-lactamases (ESBLs) (blaSHV-12 and blaCTX-M-9a) and AmpC (blaMIR) were encoded on the chromosome, and the pY546 plasmid contained several clusters of genes conferring resistance to metals, such as copper (pco), arsenic (ars), tellurite (ter), and tetrathionate (ttr), and genes encoding many divalent cation transporter proteins. The comparative genomic analyses of the whole plasmid sequence and of the heavy metal resistance gene-encoding regions revealed that the plasmid sequences of Klebsiella pneumoniae (such as pKPN-332, pKPN-3967, and pKPN-262) shared the highest similarity with those of pY546. …”
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Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440 by Jakob Grether, Holger Dittmann, Leon Willems, Tabea Schmiegelt, Elvio Henrique Benatto Perino, Philipp Hubel, Lars Lilge, Rudolf Hausmann

“…Proteomics indicated a remodeling of the respiratory chain and the regulation of stress-related proteins under oxygen limitation. Of the three promoters tested in fluorescent biosensor assays, the promoter of the oxygen-sensitive cbb3-type cytochrome c oxidase gene showed high oxygen-dependent controllability. …”
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<i>Syntrichia laevipila</i> Brid., a Bryophyta from Northwest Argentina as a Source of Antioxidants and Antimicrobials by Luis Ignacio Jiménez, Florencia Maria Correa Uriburu, José Javier Martínez Chamás, Guillermo Martin Suárez, Iris Catiana Zampini, Mario J. Simirgiotis, María Inés Isla

“…The determination of reducing and total sugars yielded 0.22 ± 0.03 mg GE/mL and 1.26 ± 0.24 mg GE/mL, respectively, while the concentration of soluble proteins was 90.60 ± 4.50 µg BSAE/mL. The extract exhibited antioxidant properties by scavenging ABTS^•+, H₂O₂, AAPH, and HO^• radicals. …”
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Virus adaptation to heparan sulfate comes with capsid stability tradeoff by Han Kang Tee, Simon Crouzet, Arunima Muliyil, Gregory Mathez, Valeria Cagno, Matteo Dal Peraro, Aleksandar Antanasijevic, Sophie Clément, Caroline Tapparel

“…When propagated in cell lines, certain viruses acquire positively charged amino acids on their surface proteins, enabling them to utilize negatively charged heparan sulfate (HS) as an attachment receptor. …”
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Impact of pubertal timing on growth progression and final height in subjects affected by RASopathies by Federica Tamburrino, Laura Mazzanti, Dino Gibertoni, Concetta Schiavariello, Annamaria Perri, Eleonora Orlandini, Cesare Rossi, Marco Tartaglia, Marcello Lanari, Emanuela Scarano

“…BackgroundRASopathies, including Noonan syndrome and related disorders, are multisystem conditions caused by mutations in various genes encoding proteins involved in the RAS/MAPK signaling pathway resulting in increased signal flow. …”
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Oridonin: A natural terpenoid having the potential to modulate apoptosis and survival signaling in cancer by Abhishek Chauhan, Hemant Joshi, Divya Kandari, Diwakar Aggarwal, Ritu Chauhan, Hardeep Singh Tuli, Arpit Mehrotra, Abhilasha Sood, Ujjawal Sharma, Darin Mansor Mathkor, Shafiul Haque, Naveen Chandra Joshi, Laurent Dufossé

“…Results: Oridonin induces apoptosis through intrinsic and extrinsic pathways, involving ROS amplification and mitochondrial membrane potential regulation. It modulates proteins like Bax, Bcl-2, cytochrome c, and caspases, leading to DNA fragmentation. …”
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Scrutinizing the evidence of anthracene toxicity on adrenergic receptor beta-2 and its bioremediation by fungal manganese peroxidase via in silico approaches by Muhammad Naveed, Khadija Khatoon, Tariq Aziz, Rida Naveed, Muhammad Nouman Majeed, Maida Salah Ud Din, Tayyab Javed, Ayaz Ali Khan, Abdullah F. Alasmari

“…The tertiary structure of both proteins was predicted using the SWISSMODEL tool and molecular docking using Autodock vina revealed strong binding affinities of anthracene with adrenergic receptor beta 2, showing a binding energy of − 6.6 kcal/mol with anthracene confirming the negative impacts on human health. …”
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DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage by Qiao Yang, Yaya Feng, Lizhen Liu, Linlin Yang, Mingshu Wang, Ying Wu, Bin Tian, Xumin Ou, Renyong Jia, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Juan Huang, Di Sun, Yu He, Zhen Wu, Ling Zhang, Yanling Yu, Anchun Cheng

“…Previous studies have shown that herpes simplex virus 1 (HSV-1) pUL15 can enter the nucleus without other viral proteins and help other terminase subunits enter the nucleus. …”
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A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat by Sandrine Nkoubat Tchoundjwen, Armel Georges Kamgang Tchawou, Clémence Mvongo, Adamou Mfopa, Joseph Ngakou Mukam, Paul Aimé Noubissi, Gaetan Olivier Fankem, René Kamgang, Jean Louis Essame Oyono

“…This study aimed to evaluate the effects of a Cameroon-comparable fat diet on visceral obesity in rats.Two groups of male Wistar rats were fed for four months with respectively a normal diet (ND) (3400 kcal/kg of food) and a high-fat diet (HFD) containing maize, cassava, palm oil, and sugar (MACAPOS 2): 35 % carbohydrate, 55 % fat and 10 % proteins (4730 kcal/kg of food). Lee index, body weight, food intake, blood and hepatic lipids, body fat, insulin resistance, glucose tolerance, glycemia, serum insulin, leptin, and adiponectin were evaluated.HFD significantly (P < 0.01) increased body weight and decreased food intake. …”
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Baicalin Liposome Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Inhibiting TLR4/JNK/ERK/NF-κB Pathway by Yu Long, Yan Xiang, Songyu Liu, Yulu Zhang, Jinyan Wan, Qiyue Yang, Mingquan Cui, Zhimin Ci, Nan Li, Wei Peng

“…Pharmacodynamics studies showed that BA-LP had a better effect than the BA group at the same dosage on reducing the W/D ratio, alleviating the lung injury score, and decreasing the proinflammatory factors (TNF-α, IL-1β) and total proteins in bronchoalveolar lavage fluids (BALF). In addition, the therapeutic effects of BA-LP showed a dose-dependent manner. …”
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The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles by Daniele R. Pereira, Yunys Pérez-Betancourt, Bianca C. L. F. Távora, Geraldo S. Magalhães, Ana Maria Carmona-Ribeiro, Eliana L. Faquim-Mauro

“…<b>Background/Objective</b>: Cationic polymers were shown to assemble with negatively charged proteins yielding nanoparticles (NPs). Poly-diallyl-dimethyl-ammonium chloride (PDDA) combined with ovalbumin (OVA) yielded a stable colloidal dispersion (OVA/PDDA-NPs) eliciting significant anti-OVA immune response. …”
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Insight on the Genetics of Atrial Fibrillation in Puerto Rican Hispanics by Ariel F. Gonzalez-Cordero, Jorge Duconge-Soler, Hilton Franqui-Rivera, Roberto Feliu-Maldonado, Abiel Roche-Lima, Israel Almodovar-Rivera

“…Some of these SNPs are proteins involved in lysosomal activities responsible for breaking ceramides to sphingosines and collagen deposition around atrial cardiomyocytes. …”
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Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates depression-like behaviour in female mice after a mild chronic stress paradigm by Christa Hohoff, Nicole Kerkenberg, Mingyue Zhang, Weronika Palkowska, Lydia Wachsmuth, Maja Peng, Lena Stiehl, Christiane Schettler, Johannes C. S. Zang, Andreas Huge, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang

“…ZDHHC7 might modulate also the response to CS via palmitoylation of sex steroid hormone receptors and other proteins critical for neuronal structure and functions. …”
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