Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression by V. E. Tvorogova, E. Y. Krasnoperova, A. A. Kudriashov, K. A. Kuznetsova, E. A. Potsenkovskaya, Y. A. Fedorova, L. A. Lutova

“…Among the SE regulators, proteins of the WOX family play significant roles. …”
Get full text

Frameshift variation in the HMG-CoA reductase gene and unresponsiveness to cholesterol-lowering drugs in type 2 diabetes mellitus patients by Esmat Khaleqsefat, Khder Hussein Rasul, Ramiar Kamal Kheder, Sonia Baban, Jamil Baban

“…As a result, translation was prematurely terminated, leading to the production of truncated proteins.…”
Get full text

Multi-stage structure-based virtual screening approach combining 3D pharmacophore, docking and molecular dynamic simulation towards the identification of potential selective PARP-1... by Mahmoud A. El Hassab, Wagdy M. Eldehna, Ghaneya S. Hassan, Sahar M. Abou-Seri

“…Several enzymes and proteins have been discovered as highly attractive targets for cancer treatment. …”
Get full text

Identification of multicohort-based predictive signature for NMIBC recurrence reveals SDCBP as a novel oncogene in bladder cancer by Chen Zhang, Hubin Yin, Tinghao Li, Junrui Chen, Weiyang He, Ke Ren, Bo Li, Xudong Liu

“…We integrated the two proteins with clinical factors to develop a nomogram model, which showed superior accuracy compared to individual parameters. …”
Get full text

Indirect determination of FMDV 146S component concentration in non-inactivated suspension by comparison of graphs of the second derivative for real-time RT-PCR curves by M. I. Doronin, D. V. Mikhalishin, V. A. Starikov, D. A. Lozovoy, A. V. Borisov

“…The main advantage of the proposed method is the capacity to determine the concentration of FMDV 146S component in a suspension with a high level of ballast proteins (more than 7.00 mg/cm3) and complete viral particles (from 0.01 to 5.00 μg/cm3).…”
Get full text

Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> by Yuxuan Jin, Shuting Zhou, Zhihui Du, Weize Wang, Zhilin Chen

“…DcTPSb1, located in the chloroplasts, was identified as a member of the TPS-b subfamily associated with monoterpenes synthesis, showing close phylogenetic relationships with homologous proteins in related plant species. An analysis of the promoter region of <i>DcTPSb1</i> indicated that it may be regulated by methyl jasmonate (MeJA) responsiveness. …”
Get full text

Combined anti-leukemic effect of gilteritinib and GSK-J4 in FLT3-ITD+ acute myeloid leukemia by Qi Zhou, Yongyu Guan, Pingping Zhao, Huiyuan Chu, Yaming Xi

“…At the same time, GSK-J4 enhances the expression of apoptosis-related proteins (Bax and cleavage caspase-9). In addition, gilteritinib or GSK-J4 monotherapy increases reactive oxygen species (ROS) production, and the combination has a synergistic effect, accelerating leukemic cell death. …”
Get full text

Enzymes enabling the biosynthesis of various C20 polyunsaturated fatty acids in a sea urchin Hemicentrotus pulcherrimus by Yingying Peng, Yutaka Haga, Naoki Kabeya

“…Specifically, 3 fatty acid desaturases (FadsA, FadsC1 and FadsC2) and 13 elongation of very-long-chain fatty acids proteins (Elovl-like, Elovl1/7-like, Elovl2/5-like, Elovl4-like, Elovl8-like and Elovl6-like A–H) were identified in their genome and transcriptomes. …”
Get full text

Remote Inflammatory Preconditioning Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Inhibition of Intrinsic Apoptosis in Rats by Yong Liu, Jiahang Xu, Liang Zhao, Jing Cheng, Baojun Chen

“…Apoptotic cells were reduced significantly by the RInPC, with the simultaneous improvement of apoptosis-related proteins. Reduction of MPO and MDA and increasing of SOD activity were found significantly improved by the RInPC. …”
Get full text

The Effect of Mesenchymal Stem Cell-Derived Exosomes and miR17-5p Inhibitor on Multicellular Liver Fibrosis Microtissues by Farnaz Sani, Mina Soufi Zomorrod, Negar Azarpira, Masoud Soleimani

“…According to the suppression of the TGF-β1 pathway, LX2 activation was inhibited, reducing extracellular matrix proteins, including COL I and α-SMA. Also, miR-17-5p expression was elevated in fibrosis conditions. …”
Get full text

Peroxisome Proliferator-Activated Receptor γ Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy by Kexing Hao, Jing Wang, Hengbin Yu, Lei Chen, Weibin Zeng, Zhengrong Wang, Guangdong Hu

“…After 12 h, 24 h, and 48 h of drug treatment, the levels of autophagy-related proteins were detected by Western blot, the triglyceride content and MDA level of cells were detected by colorimetry, and the lipid droplets and lysosomes were localized by immunofluorescence. …”
Get full text

Molecular mechanisms and comparative transcriptomics of diapause in two corn rootworm species (Diabrotica spp.) by Melise C. Lecheta, Chad Nielson, B. Wade French, Emily A.W. Nadeau, Nicholas M. Teets

“…Diapause initiation involves shutdown of the cell cycle by downregulating cyclin-related genes, downregulation of aerobic metabolism, with concurrent upregulation of stress-related genes, especially heat shock proteins, the proteasome, and immune-related genes. …”
Get full text

Candidate genes for productivity identified by genome-wide association study with indicators of class in the Russian meat merino sheep breed by A. Y. Krivoruchko, O. A. Yatsyk, E. Y. Safaryan

“…The listed genes encode proteins involved in the control of the cell cycle and DNA replication, regulation of cell proliferation and apoptosis, lipid and carbohydrate metabolism, the development of the inflammatory process and the work of circadian rhythms. …”
Get full text

Screening approaches and potential of isolated lactic acid bacteria for improving fermentation of Saccharina latissima by Evangelia Zioga, Susan Løvstad Holdt, Fredrik Gröndahl, Claus Heiner Bang-Berthelsen

“…Although the results regarding proteolytic capacity were not sufficient to prove that the candidates can also provide some flavor generation by the cleavage of proteins, NFICC1746 and NFICC2041 exhibited potential in releasing free alanine, glutamate and asparate as free amino acids. …”
Get full text

SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells by Linli Li, Xifeng Liu, Bipin Gaihre, Sungjo Park, Yong Li, Andre Terzic, Lichun Lu

“…SDF-1α released from the composites significantly promoted migration and osteogenic differentiation of MSCs documented by upregulated expression of osteogenic-related proteins, ALP, Runx2, OCN, and OPN. SDF-1α at 100 ng/ml was optimal for enhanced migration and osteogenic proficiency. …”
Get full text

Model systems of human immunodef iciency virus (HIV-1) for in vitro eff icacy assessment of candidate vaccines and drugs against HIV-1 by N. B. Rudometova, D. N. Shcherbakov, A. P. Rudometov, A. A. Ilyichev, L. I. Karpenko

“…The classical method of virology, which makes it possible to evaluate the neutralizing activity of the sera of animals immunized with experimental vaccines and the eff icacy of chemotherapy agents is the method of neutralization using viral isolates and infectious molecular clones, i. e. infectious viral particles obtained via cell transfection with a plasmid vector including the full-length HIV-1 genome coding structural, regulatory, and accessory proteins of the virus required for the cultivation of replication-competent viral particles in cell culture. …”
Get full text

Complex immunotoxic effects of T-2 Toxin on the murine spleen and thymus: Oxidative damage, inflammasomes, apoptosis, and immunosuppression by Mingyan Chen, Yanfang Zhang, Yangbo Zhao, Kewei Cao, Ruiyan Niu, Dongguang Guo, Zilong Sun

“…Furthermore, the levels of NLRP3, ASC, caspase-1, and IL-1β proteins were significantly elevated, indicating the activation of the NLRP3 inflammasome pathway. …”
Get full text

Ocular TGF-β, Matrix Metalloproteinases, and TIMP-1 Increase with the Development and Progression of Diabetic Retinopathy in Type 2 Diabetes Mellitus by Lucia Saucedo, Isabel B. Pfister, Souska Zandi, Christin Gerhardt, Justus G. Garweg

“…Similarly, an increase in MMP-3 and TIMP-1 levels in the AH was evident in the later stages of DR, corresponding to a 7.7- and 2.4-fold increase compared to the control group, respectively, whereas serum levels of the studied proteins remained similar. In conclusion, increased concentrations of TGF-β1, MMP-3, and TIMP-1 in the AH, but not in the serum, in advanced NPDR/PDR indicate that the intraocular regulation for these cytokines is independent of the systemic one and suggest their involvement in the progression of DR.…”
Get full text

Evaluation of Nutritive and Antioxidant Properties of Blanched Leafy Vegetables Consumed in Northern Côte d’Ivoire by Oulai Patricia D., Zoué Lessoy T., Niamké Sébastien L.

“…The registered losses (p0.05) at 15 min were as follow: ash (0.08-10.01%), proteins (0.36-12.03%), vitamin C (19.56-68.67%), carotenoids (18.91-55.48%) oxalates (3.58-21.39%) and phytates (10.51-68.02%). …”
Get full text

Effect of cubebin against streptozotocin-induced diabetic nephropathy rats via inhibition TNF-α/NF-κB/TGF-β: in vivo and in silico study by Rahamat Unissa Syed, Sivakumar S. Moni, Weiam Hussein, Taghreed Mohammad Saad Alhaidan, Sondos Mohammed Y. Abumilha, Lama Khalid Alnahdi, Ling Shing Wong, Vetriselvan Subramaniyan, Vinoth Kumarasamy

“…The results of MDS provided insight into the mechanisms that associate proteins TNF-α, NF-κB, and TGF-β1 in conformational dynamics upon binding to cubebin. …”
Get full text