The effect of salicylic and jasmonic acids on the activity of <i>SnAGO</i> genes in the fungus <i>Stagonospora nodorum</i> Berk. in <i>in vitro</i> culture and during infection o... by M. Yu. Shein, G. F. Burkhanova, I. V. Maksimov

“…RNA interference is a gene silencing mechanism that plays an important role in genetic regulation in a number of eukaryotes. Argonaute (AGO) proteins are central to the complex RNA interference system. …”
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CHAMP1 premature termination codon mutations found in individuals with intellectual disability cause a homologous recombination defect through haploinsufficiency by Yujiro Yoshizaki, Yunosuke Ouchi, Dicky Kurniawan, Eisuke Yumoto, Yuki Yoneyama, Faiza Ramadhani Rizqullah, Hiyori Sato, Mirjam Hanako Sarholz, Toyoaki Natsume, Masato T. Kanemaki, Masanori Ikeda, Ayako Ui, Kenji Iemura, Kozo Tanaka

“…In Epstein-Barr virus-induced lymphoblastoid cells and fibroblasts derived from individuals with CHAMP1 PTC mutations, truncated CHAMP1 proteins of the expected sizes were detected. When DSBs were induced in fibroblasts with PTC mutations, a defect in HR was detected. …”
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Effects of a Novel Glucokinase Activator, HMS5552, on Glucose Metabolism in a Rat Model of Type 2 Diabetes Mellitus by Ping Wang, Huili Liu, Li Chen, Yingli Duan, Qunli Chen, Shoumin Xi

“…The expression patterns of proteins and genes associated with insulin resistance and GK activity were assayed. …”
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Human Placenta-Derived Mesenchymal Stem Cells Reduce Mortality and Hematoma Size in a Rat Intracerebral Hemorrhage Model in an Acute Phase by Bo Young Choi, Ok Joon Kim, Sae-Hong Min, Jeong Hyun Jeong, Sang Won Suh, Tae Nyoung Chung

“…Expression of tight junction proteins (ZO-1, occludin) and coagulation factor VII mRNA was also compared. …”
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Detection of fusion events by RNA sequencing in FFPE versus freshly frozen colorectal cancer tissue samples by Maxim Sorokin, Maxim Sorokin, Maxim Sorokin, Maxim Sorokin, Vladimir Lyadov, Vladimir Lyadov, Vladimir Lyadov, Maria Suntsova, Marat Garipov, Anna Semenova, Natalia Popova, Egor Guguchkin, Rustam Heydarov, Marianna Zolotovskaia, Xiaowen Zhao, Qing Yan, Ye Wang, Evgeny Karpulevich, Anton Buzdin, Anton Buzdin, Anton Buzdin, Anton Buzdin

“…Gene fusion events result in chimeric proteins that are frequently found in human cancers. …”
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Advancing Alzheimer's Therapy: Computational strategies and treatment innovations by Jibon Kumar Paul, Abbeha Malik, Mahir Azmal, Tooba Gulzar, Muhammad Talal Rahim Afghan, Omar Faruk Talukder, Samar Shahzadi, Ajit Ghosh

“…Case studies highlight the application of computational techniques in AD therapeutics, including the suppression of crucial proteins implicated in disease progression and the repurposing of existing drugs for AD management. …”
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Novel adaptive immune systems in pristine Antarctic soils by Marc W. Van Goethem, Oliver K. I. Bezuidt, Rian Pierneef, Surendra Vikram, David W. Hopkins, Thomas Aspray, Grant Hall, Stephan Woodborne, Ian D. Hogg, Trent R. Northen, Weidong Kong, Daniele Daffonchio, Don A. Cowan, Yves Van de Peer, Manuel Delgado-Baquerizo, Thulani P. Makhalanyane

“…Phylogenetic analysis of Cas12 effector proteins hints at divergent evolutionary histories compared to classified type V effectors and indicates that TnpB is likely the ancestor of Cas12 nucleases. …”
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The Pharmacodynamic Impact of Apremilast, an Oral Phosphodiesterase 4 Inhibitor, on Circulating Levels of Inflammatory Biomarkers in Patients with Psoriatic Arthritis: Substudy Res... by Peter H. Schafer, Peng Chen, Lorraine Fang, Andrew Wang, Rajesh Chopra

“…Of 504 patients randomized in PALACE 1, 150 (placebo: n=51; apremilast 20 mg BID: n=51; apremilast 30 mg BID: n=48) provided peripheral blood plasma samples for analysis in a multiplexed cytometric bead array assay measuring 47 proteins associated with systemic inflammatory immune responses. …”
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Itraconazole promotes melanoma cells apoptosis via inhibiting hedgehog signaling pathway-mediated autophagy by Shunqiao Jin, Shunqiao Jin, Xiaojiao Liu, Xiaojiao Liu, Lingqin Cai, Lingqin Cai, Jiayu Yan, Ling Li, Hongjun Dong, Yuxue Gao, Xicong Zhu, Cong Zhang, Xuezhu Xu

“…Western blot analysis was performed to examine autophagy and associated signaling proteins, while autophagy flux and autophagosome formation were visualized using fluorescence microscopy. …”
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Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells by Yuzhou Gu, Yuzhou Gu, Yuzhou Gu, Kexin Wu, Kexin Wu, Kexin Wu, Bowen Niu, Bowen Niu, Bowen Niu, Zhiting Wang, Zhiting Wang, Zhiting Wang, Yuchen Jie, Yuchen Jie, Yuchen Jie, Zixuan Fan, Zixuan Fan, Zixuan Fan, Junying Li, Junying Li, Congjiao Sun, Congjiao Sun, Congjiao Sun, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Li-Wa Shao, Li-Wa Shao, Li-Wa Shao

“…A gonad-derived PGC line of White Leghorn (WLH) chicken was established and verified through PAS staining and immunofluorescence of PGC-specific proteins. To visually observe PGC migration in vivo, GFP-positive PGCs were prepared and locations of chimeras were validated. …”
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Nobiletin restores the intestinal barrier of HFD-induced obese mice by promoting MHC-II expression and lipid metabolism by Ni Yang, Yue-Shan Pang, Yali Zheng, Yan-Ju Gong, Wei-Jun Ding

“…The results revealed that intestinal tight proteins, including ZO-1 and Occludin, were significantly reduced in HFD-fed mice but markedly restored after nobiletin intervention, particularly in NOH mice. …”
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Achalasia—An Autoimmune Inflammatory Disease: A Cross-Sectional Study by J. Furuzawa-Carballeda, D. Aguilar-León, A. Gamboa-Domínguez, M. A. Valdovinos, C. Nuñez-Álvarez, L. A. Martín-del-Campo, A. B. Enríquez, E. Coss-Adame, A. E. Svarch, A. Flores-Nájera, A. Villa-Baños, J. C. Ceballos, G. Torres-Villalobos

“…Achalasia tissue exhibited an increase in the expression of proteins involved in extracellular matrix turnover, apoptosis, proinflammatory and profibrogenic cytokines, and Tregs and Bregs versus controls (P<0.001). …”
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Selective Immunomodulation of Inflammatory Pathways in Keratinocytes by the Janus Kinase (JAK) Inhibitor Tofacitinib: Implications for the Employment of JAK-Targeting Drugs in Psor... by Martina Morelli, Claudia Scarponi, Laura Mercurio, Francesco Facchiano, Sabatino Pallotta, Stefania Madonna, Giampiero Girolomoni, Cristina Albanesi

“…Tofacitinib is an inhibitor of JAK proteins, which, similarly to SOCS, impedes JAK phosphorylation. …”
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Reprogramming of iPSCs to NPCEC-like cells by biomimetic scaffolds for zonular fiber reconstruction by Tianhui Chen, Zhongxing Chen, Juan Du, Min Zhang, Zexu Chen, Qingyi Gao, Aodong Chen, Qinghao Meng, Yang Sun, Yan liu, Linghao Song, Xinyue Wang, Pranav Prakash Edavi, Chen Xu, Hongmei Zhang, Jinhai Huang, Yongxiang Jiang

“…Ocular tissue and aqueous humour samples from patients with EL were subjected to RNA sequencing and Olink high-throughput proteomic analysis, revealing the downregulation of pathogenic genes (FBN1, MFAP2) and upregulation of secretory proteins (IL-12, MMP-1). The high expression of FBN1 and MFAP2 in NPCECs suggests their potential as candidates for zonular fiber construction; however, the limited availability of donor sources restricts the feasibility of NPCEC transplantation therapy. …”
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Chemoproteomic analysis of the promising candidate molecule of the indole derivative with lab code SV-1010 and other non-steroidal anti-inflammatory drugs by Pavel A. Galenko-Yaroshevsky, Ivan Yu. Torshin, Andrey N. Gromov, Ivan A. Reyer, Olga A. Gromova, Tereza R. Glechyan, Konstantin F. Suzdalev, Andrey V. Zadorozhniy, Anait V. Zelenskaya

“…Results: The significant differences in the effects of the studied molecules were found for 820 proteins of the rat proteom. SV-1010, to a lesser degree than the other molecules, can inhibit dopamine D1- and D2-type receptors and, at the same time, stimulate the release of dopamine in the neostriatum (EC50 = 27 nM). …”
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Bioactive Potential of Some <i>Bacillus thuringiensis</i> Strains from Macapá, Amazon, Brazil, Against the Housefly <i>Musca domestica</i> (Diptera: Muscidae) Under Laboratory Cond... by Tatiane Aparecida Nascimento, Maria José Paes, Fernando Hercos Valicente, Margareth Maria de Carvalho Queiroz

“…PCR was employed to detect genes encoding Cry and Cyt proteins and Vips. Six strains exhibited 70 to 100% larval mortality, with five containing <i>cry</i> genes, none harboring <i>cyt</i> genes, and four carrying <i>vip</i> genes. …”
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Nanofertilizers benefited maize to cope oxidative stress under saline environment by Abbas Shoukat, Zulfiqar Ahmad Saqib, Allah Nawaz, Kashif Zulfiqar Amir, Ilyas Ahmad, Ali Hamza, Karl Hermann Mühling

“…Higher proline content, proteins, relative water and chlorophyll content in plants treated with nano fertilizers demonstrated improved osmoregulation, metabolic homeostasis as well as maintaining water balance and photosynthetic efficiency under stress conditions. …”
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Development of the test kit for detection of SARS-CoV-2 antibodies in sera of susceptible animals by M. A. Volkova, N. G. Zinyakov, P. S. Yaroslavtseva, I. A. Chvala, T. S. Galkina, D. B. Andreychuk

“…The use of a purified concentrated inactivated virus as an antigen allows the detection of antibodies to various SARS-CoV-2 immunodominant proteins (S and N). The reaction conditions were optimized, and a positive-negative threshold was established by testing of 154 negative sera from animals of six species (ferrets, minks, foxes, arctic foxes, cats and dogs). …”
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Binding and cleavage of pro-urokinase by a tegument extract of Fasciola hepatica newly excysted juveniles activate the host fibrinolytic system by Judit Serrat, María Torres-Valle, Carolina De Marco Verissimo, Mar Siles-Lucas, Javier González-Miguel

“…We found that F. hepatica cathepsins B3, L3, enolase and glutathione S-transferase mediate this interaction, suggesting a multifactorial or moonlighting role for these proteins. Additionally, our results revealed that the tegument of FhNEJ contains a protease that is capable of cleaving and activating pro-u-PA into its catalytically active form, which positively impacts the capacity of the parasites to generate plasmin from the host PLG. …”
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Genome-Wide Analyses Reveal the Roles of CCT Genes as Regulators of Abiotic Stress Responses in <i>Citrullus lanatus</i> by Yajie Hong, Mengsha Li, Wona Ding, Jun Shi, Zishuo Zheng, Nailin Xing, Qiuping Li

“…Functional diversity was also evident among CCT family genes within a given species as well as among species attributable to changes in the structural features and expression patterns of the genes and the encoded proteins.…”
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