H2AX Phosphorylation: Its Role in DNA Damage Response and Cancer Therapy by Monika Podhorecka, Andrzej Skladanowski, Przemyslaw Bozko

“…Phosphorylation of H2AX plays a key role in DDR and is required for the assembly of DNA repair proteins at the sites containing damaged chromatin as well as for activation of checkpoints proteins which arrest the cell cycle progression. …”
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Effects of exosomes from human dental pulp stem cells on the biological behavior of human fibroblasts by Guan-Yu Chen, Ling-ling Fu, Hui-ping Ye, Ping Cheng, Hong-chao Feng, Ming Yan

“…In addition, DPSCs-Exos was able to inhibit the expression of fibrosis-related genes and proteins in KFs. This study highlights the role of DPSCs-Exos in regulating the biological behaviour of fibroblasts, providing new insights for future applications in the field of cell-free regenerative medicine.…”
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Measuring plant cysteine oxidase interactions with substrates using intrinsic tryptophan fluorescence by Dona M. Gunawardana, Daisy A. Southern, Emily Flashman

“…Abstract Plant Cysteine Oxidases (PCOs) are oxygen-sensing enyzmes that catalyse oxidation of cysteinyl residues at the N-termini of target proteins, triggering their degradation via the N-degron pathway. …”
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Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover. by Shoujun Huang, Zhao Zhang, Chunxia Zhang, Xiangdong Lv, Xiudeng Zheng, Zhenping Chen, Liwei Sun, Hailong Wang, Yuanxiang Zhu, Jing Zhang, Shuyan Yang, Yi Lu, Qinmiao Sun, Yi Tao, Feng Liu, Yun Zhao, Dahua Chen

“…Hedgehog signaling has an unusual reception system consisting of two transmembrane proteins, Patched receptor and Smoothened signal transducer. …”
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PRDX5 and PRDX6 translocation and oligomerization in bull sperm: a response to cryopreservation-induced oxidative stress by Mostek-Majewska Agnieszka, Bossowska-Nowicka Magdalena, Słowińska Mariola, Ciereszko Andrzej

“…Under cryopreservation, these proteins formed high molecular weight oligomers, that may shift from peroxidase to chaperone roles. …”
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BlockingαVβ3 Integrin Ligand Occupancy Inhibits the Progression of Albuminuria in Diabetic Rats by Laura A. Maile, Katherine Gollahon, Christine Wai, Paul Dunbar, Walker Busby, David Clemmons

“…Treatment with the antibody prevented the increase of several profibrotic proteins that have been implicated in the development of DN. …”
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Rhesus Cytomegalovirus-encoded Fcγ-binding glycoproteins facilitate viral evasion from IgG-mediated humoral immunity by Claire E. Otero, Sophia Petkova, Martin Ebermann, Husam Taher, Nessy John, Katja Hoffmann, Angel Davalos, Matilda J. Moström, Roxanne M. Gilbride, Courtney R. Papen, Aaron Barber-Axthelm, Elizabeth A. Scheef, Richard Barfield, Lesli M. Sprehe, Savannah Kendall, Tabitha D. Manuel, Teresa Beechwood, Linh Khanh Nguyen, Nathan H. Vande Burgt, Cliburn Chan, Michael Denton, Zachary J. Streblow, Daniel N. Streblow, Alice F. Tarantal, Scott G. Hansen, Amitinder Kaur, Sallie Permar, Klaus Früh, Hartmut Hengel, Daniel Malouli, Philipp Kolb

“…Since vFcγRs were not required for superinfection of rhesus macaques, we conclude that these proteins can prolong lytic replication during primary infection by evading virus-specific adaptive immune responses, particularly antibodies.…”
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Role of Epigenetic Histone Modifications in Diabetic Kidney Disease Involving Renal Fibrosis by Jing Sun, Yangwei Wang, Wenpeng Cui, Yan Lou, Guangdong Sun, Dongmei Zhang, Lining Miao

“…Renal fibrosis, characterized by the accumulation of extracellular matrix (ECM) proteins in glomerular basement membranes and the tubulointerstitium, is the final manifestation of DKD. …”
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CRISPR/Cas Enzyme Catalysis in Liquid–Liquid Phase‐Separated Systems by Yaqin Zhang, Jianai Chen, Zhina Wu, Chenfei Zhao, Rui Wang, Zhiping Li, Jiasi Wang, Di Wang

“…Abstract The clustered regularly interspaced palindromic repeats (CRISPR) /CRISPR‐associated proteins (Cas) system is the immune system in bacteria and archaea and has been extensively applied as a critical tool in bioengineering. …”
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Growth Phase-Dependent Proteomes of the Malaysian Isolated Lactococcus lactis Dairy Strain M4 Using Label-Free Qualitative Shotgun Proteomics Analysis by Theresa Wan Chen Yap, Amir Rabu, Farah Diba Abu Bakar, Raha Abdul Rahim, Nor Muhammad Mahadi, Rosli Md. Illias, Abdul Munir Abdul Murad

“…A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. …”
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Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices by Julie Allyson, Eve Dontigny, Yves Auberson, Michel Cyr, Guy Massicotte

“…In the present study, we used selective NMDA receptor antagonists to investigate the involvement of NR2A and NR2B subunits in the modulatory effect of basal NMDA receptor activity on the phosphorylation of Tau proteins. We observed, in acute hippocampal slice preparations, that blockade of NR2A-containing NMDA receptors by the NR2A antagonist NVP-AAM077 provoked the hyperphosphorylation of a residue located in the proline-rich domain of Tau (i.e., Ser199). …”
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Sarcoglycans are enriched at the neuromuscular junction in a nerve-dependent manner by Michela Gloriani, Bianca Cheli, Chiara D’Ercole, Veronica Ruggieri, Marianna Cosentino, Mireia Serrat Pineda, Biliana Lozanoska-Ochser, Francesca Grassi, Marina Bouché, Luca Madaro, Carles Sánchez Riera

“…Here, we show that SGC proteins are enriched at the post-synaptic membrane of neuromuscular junctions (NMJs). …”
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Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient by Rosalinda Yasato Camargo, Cristina Takami Kanamura, Celso Ubirajara Friguglietti, Célia Regina Nogueira, Sonia Iorcansky, Alfio José Tincani, Ana Karina Bezerra, Ester Brust, Fernanda Christtanini Koyama, Anamaria Aranha Camargo, Fernanda Orpinelli R. Rego, Pedro Alexandre Favoretto Galante, Geraldo Medeiros-Neto, Ileana Gabriela Sanchez Rubio

“…Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. …”
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Proteomic Analysis of the Murine Liver Response to Oral Exposure to Aflatoxin B1 and Ochratoxin A: The Protective Role to Bioactive Compounds by Silvia Trombetti, Alessandra Cimbalo, Michela Grosso, Pilar Vila-Donat, Jordi Mañes, Lara Manyes

“…Gene ontology visualization allowed the identification of proteins involved in important biological processes such as the response to xenobiotic stimuli and liver development. …”
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Genetic and molecular mechanisms of hydrocephalus by Xuehai Deng, Xuehai Deng, Yiqian Chen, Yiqian Chen, Qiyue Duan, Qiyue Duan, Jianlin Ding, Jianlin Ding, Zhong Wang, Zhong Wang, Junchi Wang, Xinlong Chen, Liangxue Zhou, Long Zhao

“…Various genetic and molecular abnormalities contribute to the mechanisms of hydrocephalus, including gene deletions or mutations, the activation of cellular inflammatory signaling pathways, alterations in water channel proteins, and disruptions in iron metabolism. Several studies have demonstrated that modulating the expression of key proteins, including TGF-β, VEGF, Wnt, AQP, NF-κB, and NKCC, can significantly influence the onset and progression of hydrocephalus. …”
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Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy by Xuejiao Tian, Tzu-Yang Lin, Po-Ting Lin, Min-Ju Tsai, Hsin Chen, Wen-Jie Chen, Chia-Ming Lee, Chiao-Hui Tu, Jui-Cheng Hsu, Tung-Han Hsieh, Yi-Chung Tung, Chien-Kai Wang, Suewei Lin, Li-An Chu, Fan-Gang Tseng, Yi-Ping Hsueh, Chi-Hon Lee, Peilin Chen, Bi-Chang Chen

“…Abstract Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixation in the nervous system. …”
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The Evolution of Immunosuppressive Therapy in Pig-to-Nonhuman Primate Organ Transplantation by S. A. Sanatkar, K. Kinoshita, A. Maenaka, H. Hara, D. K. C. Cooper

“…Major steps were the identification and deletion of expression of the three known glycan xenoantigens on pig vascular endothelial cells, the transgenic expression of human “protective” proteins, e.g., complement-regulatory, coagulation-regulatory, and anti-inflammatory proteins, and the administration of an immunosuppressive regimen based on blockade of the CD40/CD154 T cell co-stimulation pathway. …”
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Proximity Labeling-Based Identification of MGAT3 Substrates and Revelation of the Tumor-Suppressive Role of Bisecting GlcNAc in Breast Cancer via GLA Degradation by Bowen Wang, Xin He, Yue Zhou, Zengqi Tan, Xiang Li, Feng Guan, Lei Lei

“…This approach enriched 116 and 189 proteins, respectively, identifying 17 common substrates shared with bisecting GlcNAc-bearing proteome obtained via intact glycopeptide enrichment methods. …”
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Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4 by Zhe Huang, Ming Wang, Yitian Chen, Hua Tang, Kuo Tang, Mingkuan Zhao, Wei Yang, Zhengjun Zhou, Junjie Tian, Wei Xiang, Shenjie Li, Qinglian Luo, Luotong Liu, Yanru Zhao, Tao Li, Jie Zhou, Ligang Chen

“…We further confirmed that migrasomes are enriched in extracellular matrix (ECM)-related proteins such as p21-activating kinase 4 (PAK4) and laminin alpha 4 (LAMA4). …”
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Epitope-imprinted biomaterials with tailor-made molecular targeting for biomedical applications by Youlu Diao, Jia Gao, Yue Ma, Guoqing Pan

“…Epitope imprinting strategy, an improved MIT by imprinting the epitopes of biomolecules (e.g., proteins and nucleic acids), enables to target the entire molecule through recognizing partial epitopes exposed on it, greatly expanding the applicability and simplifying synthesis process of molecularly imprinted polymers (MIPs). …”
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