Duckweed: a starch-hyperaccumulating plant under cultivation with a combination of nutrient limitation and elevated CO2 by Ling Guo, Ling Guo, Yang Fang, Songhu Wang, Yao Xiao, Yanqiang Ding, Yanling Jin, Xueping Tian, Anping Du, Zhihua Liao, Kaize He, Shuang Chen, Yonggui Zhao, Li Tan, Zhuolin Yi, Yuqing Che, Lanchai Chen, Jinmeng Li, Leyi Zhao, Peng Zhang, Zhengbiao Gu, Fangyuan Zhang, Yan Hong, Qing Zhang, Hai Zhao

“…These alterations led to a reduction in lignocellulose and protein contents and ultimately an increase in the accumulation of starch in the chloroplasts.ConclusionThis work demonstrates the potential of duckweed as a highly efficient starch producer.…”
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Interplay between oxidative stress, neuroinflammatory cytokines and melatonin in Alzheimer's disease: Insights from cerebrospinal fluid analysis by Francisco Artime-Naveda, David Hevia, Rebeca Alonso-Arias, Carmen Martínez, Isabel Quirós-González, Rafael Cernuda-Cernuda, Alejando Alvarez-Artime, Iván Menéndez-Valle, Rosa M. Sainz, Juan C. Mayo

“…CSF levels of Aβ, T-tau and P-tau were assayed, and patients were further classified according to threshold CSF levels of the three markers protein following the criteria of NIA-AA. Results: Correlational and group analysis showed a positive association between oxidative stress and neuronal damage. …”
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Therapeutic effects of platelet-derived extracellular vesicles on viral myocarditis correlate with biomolecular content by Danielle J. Beetler, Danielle J. Beetler, Danielle J. Beetler, Presley Giresi, Damian N. Di Florio, Damian N. Di Florio, Damian N. Di Florio, Jessica J. Fliess, Elizabeth J. McCabe, Molly M. Watkins, Molly M. Watkins, Molly M. Watkins, Vivian Xu, Matthew E. Auda, Katelyn A. Bruno, Katelyn A. Bruno, Emily R. Whelan, Emily R. Whelan, Emily R. Whelan, Stephen P. C. Kocsis, Brandy H. Edenfield, Sierra A. Walker, Sierra A. Walker, Sierra A. Walker, Logan P. Macomb, Kevin C. Keegan, Angita Jain, Angita Jain, Angita Jain, Andrea C. Morales-Lara, Isha Chekuri, Anneliese R. Hill, Houssam Farres, Joy Wolfram, Joy Wolfram, Atta Behfar, Atta Behfar, Paul G. Stalboerger, Andre Terzic, Andre Terzic, Leslie T. Cooper, DeLisa Fairweather, DeLisa Fairweather, DeLisa Fairweather

“…We injected PEV, pmPEV, or vehicle control in a mouse model of viral myocarditis and examined histology, gene expression, protein profiles, and performed proteome and microRNA (miR) sequencing of EVs.ResultsWe found that both PEV and pmPEV significantly inhibited myocarditis; however, PEV was more effective, which was confirmed by a greater reduction of inflammatory cells and proinflammatory and profibrotic markers determined using gene expression and immunohistochemistry. …”
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Integrating network analysis and experimental validation to reveal the mechanism of Cuscuta chinensis lam. Extract in the treatment of IgA nephropathy by Xianlong Zhang, Mingjie Liang, Ziyang Lin, Minyi Li, Tingting Duan, Yun Han, Lanqing Meng, Mengqiu Li, Guixuan Lin, Tao Xia, Ying Lai, Boen Liang, Bingqiong Li, Minhua Li, Fengxin Kang, Quan Zhu, Zhenghai Li, Junzheng Yang

“…Results: The results demonstrated that a significant decrease in the glomerular filtration rate, accompanied by a notable elevation in biochemical indexes in rats in model group, including the ratio of total protein to creatinine in urine, sCr, BUN, TG, AST, ALT, IL-1β and TNF-α; additionally, the rats in the model group exhibited substantial histopathological alterations, characterized by the presence of many IgA deposits; CCLE has been demonstrated to enhance the glomerular filtration rate, downregulate the levels of sCr, BUN, TG, AST, ALT, IL-1β and TNF-α, reduce the IgA deposition, and ameliorate the histopathological changes in IgA nephropathy rats; Western blotting demonstrated CCLE can suppress the expressions of p-PI3K, p-AKT, p-IKK, p-NF-κB, and p-IκB in IgA nephropathy rats. …”
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The combination of rAAV pseudo-lipid nanoparticle and triamcinolone acetonide enables multi-administration to liver by Chunmei Gan, Mi Leng, Yu Liu, Zhaoyue Zheng, Siwu He, Wen Qiao, Lin Xiao, Yao Xiao, Jingya Ye, Lixing Zhou, Jiao Zhou, Boduan Xiao, Wenxin Zhao, Jiamei Yang, Aohan Wu, Huiyuan Zhang, Hongbo Hu, Xiaobo Cen, Zhiyong Qian, Haohao Dong, C. Alexander Valencia, Lunzhi Dai, Hoi Yee Chow, Lei Zhang, Biao Dong

“…Notable neutralizing antibody reductions of 37.8-fold and 12.7-fold were observed by the combinatorial strategy compared with the independent LNP encapsulation and TAC treatment approaches. The plasma hFIX protein was enhanced to 15.1 μg/mL and the liver bioluminescence was elevated to 1.4 × 108 p/s/cm2/sr following the second and third administrations, with weaker levels in LNP encapsulation (1.9 μg/mL, 2.1 × 104 p/s/cm2/sr) and TAC treatment (3.0 μg/mL, 6.1 × 104 p/s/cm2/sr) groups. …”
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Development of a recombinant infectious bronchitis virus vaccine expressing infectious laryngotracheitis virus multiple epitopes by Guanming Shao, Jun Fu, Yun Pan, Shiying Gong, Chaoyi Song, Sheng Chen, Keyu Feng, Xinheng Zhang, Qingmei Xie

“…In this study, we designed a multi-epitope cassette (gD-T/B) containing multiple T and B cell epitopes of ILTV gD protein. The genetic stability of the full-length gD gene and the gD-T/B multi-epitope cassette replacing non-essential genes in IBV was systematically analyzed. …”
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Comparative analysis of the performance, egg quality and ovarian immune function of fast and slow feather strains in tianfu green shell laying hens at various stages of egg product... by Zhi Hu, Hengyong Xu, Zhipeng Zhang, Yuxiang Lu, Yuxin Zhou, Jiajun Zhu, Qingqing Deng, Xi Wang, Yiping Liu, Yao Zhang, Yan Wang

“…Under the identical feeding and management conditions, the live weight, tibial length, egg production performance, egg quality, serum biochemical indexes, immune protein content, and the expression of related genes in uterine and ovarian tissues were assessed and analyzed. …”
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Interleukin‐37 promotes DMBA/TPA skin cancer through SIGIRR‐mediated inhibition of glycolysis in CD103+DC cells by Fan‐lian Zeng, Xiao‐yan Wang, Ya‐wen Hu, Zhen Wang, Ya Li, Jing Hu, Jia‐dong Yu, Pei Zhou, Xiu Teng, Hong Zhou, Hua‐ping Zheng, Fu‐lei Zhao, Lin‐na Gu, Cheng‐cheng Yue, Shu‐wen Chen, Juan Cheng, Yan Hao, Qi‐xiang Zhao, Chen Zhang, Song Zou, Zhong‐lan Hu, Xiao‐qiong Wei, Xiao Liu, Guo‐lin Li, Nong‐yu Huang, Wen‐ling Wu, Yi‐fan Zhou, Wei Li, Kaijun Cui, Jiong Li

“…Notably, IL‐37 induced rapid phosphorylation of adenosine 5‘‐monophosphate (AMP)‐activated protein kinase (AMPK), and via single immunoglobulin IL‐1‐related receptor (SIGIRR), inhibited the long‐term Akt activation. …”
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Sodium butyrate restored TRESK current controlling neuronal hyperexcitability in a mouse model of oxaliplatin-induced peripheral neuropathic pain by Idy H.T. Ho, Yidan Zou, Kele Luo, Fenfen Qin, Yanjun Jiang, Qian Li, Tingting Jin, Xinyi Zhang, Huarong Chen, Likai Tan, Lin Zhang, Tony Gin, William K.K. Wu, Matthew T.V. Chan, Changyu Jiang, Xiaodong Liu

“…Differential expressions of histone deacetylases (HDACs) and pain-related K+ channels were quantified with rt-qPCR and protein assays. Immunofluorescence assays of histone acetylation at H3K9/14 were performed in primary DRG cultures treated with sodium butyrate. …”
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Mucosal SARS-CoV-2 S1 adenovirus-based vaccine elicits robust systemic and mucosal immunity and protects against disease in animals by Najwa D. Aljehani, Levi Tamming, Muhammad Yasir Khan, Rwaa H. Abdulal, Mohamed A. Alfaleh, Aishah Ghazwani, Asalah Helal, Reem M. Alsulaiman, Mohammad A. Sanki, Khalid Alluhaybi, Farah Ayman Sukareh, Rahaf H. Alharbi, Faris H. Alyami, M-Zaki ElAssouli, Salima Shebbo, Wesam H. Abdulaal, Abdullah Algaissi, Ahmad Bakur Mahmoud, Mohammad Basabrain, Diana Duque, Jegarubee Bavananthasivam, Wangxue Chen, Lisheng Wang, Simon Sauve, Turki S. Abujamel, Tarfa Altorki, Rowa Alhabbab, Anh Tran, Xuguang Li, Anwar M. Hashem

“…These findings suggest that IN delivery of rAd5-SARS2-S1 could be a promising approach for inducing mucosal and systemic immunity, offering enhanced protection against SARS-CoV-2 and emerging variants.IMPORTANCEThis publication presents an assessment of the immune response and effectiveness of a vaccine containing genetically modified non-replicating recombinant that expresses the S1 subunit protein of SARS-CoV-2. We conducted a comparative analysis of the immune response potency, durability, and protective effectiveness of this vaccine using intramuscular (IM) and intranasal (IN) inoculation in mice and Syrian hamsters. …”
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Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy by Mari Aikio, Hana M. Odeh, Heike J. Wobst, Bo Lim Lee, Úna Chan, Jocelyn C. Mauna, Korrie L. Mack, Bradley Class, Thomas A. Ollerhead, Alice F. Ford, Edward M. Barbieri, Ryan R. Cupo, Lauren E. Drake, Joshua L. Smalley, Yuan-Ta Lin, Stephanie Lam, Reuben Thomas, Nicholas Castello, Ashmita Baral, Jenna N. Beyer, Mohd A. Najar, John Dunlop, Aaron D. Gitler, Ashkan Javaherian, Julia A. Kaye, George M. Burslem, Dean G. Brown, Christopher J. Donnelly, Steven Finkbeiner, Stephen J. Moss, Nicholas J. Brandon, James Shorter

“…Persistent activation of p38 mitogen-activated protein kinase (MAPK) is implicated in ALS. However, it is unclear how p38 MAPK affects TDP-43 proteinopathy. …”
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Inhibition of S100A8/A9 ameliorates neuroinflammation by blocking NET formation following traumatic brain injury by Guihong Shi, Yiyao Cao, Jianye Xu, Bo Chen, Xu Zhang, Yanlin Zhu, Liang Liu, Xilei Liu, Luyuan Zhang, Yuan Zhou, Shenghui Li, Guili Yang, Xiao Liu, Fanglian Chen, Xin Chen, Jianning Zhang, Shu Zhang

“…S100A8/A9, also known as calprotectin or myeloid-related protein-8/14 (MRP8/14), is an alarmin primarily secreted by activated neutrophils with potent pro-inflammatory property. …”
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Bisphenol B restrains rat leydig cell function via H3K27me3/H3K9me3 histone modifications by Jiayi He, Huiqian Zhang, Hehua Quan, Qingyuan Wang, Congcong Wen, Yiyan Wang, Yang Zhu, Ren-Shan Ge, Xiaoheng Li

“…BPB did not alter the numbers of CYP11A1+ Leydig cells and SOX9+ Sertoli cells, but it downregulated the expression of key genes in testosterone synthesis pathway (Lhcgr, Scarb1, Star, Cyp11a1, Cyp17a1, Hsd11b1, Hsd17b3, and Insl3) and their corresponding protein levels. Notably, BPB significantly boosted the expressions of histone methylation markers like EEF1A1, SUZ12, EED, EZH2, H3K27me3, and H3K9me3 in vivo. …”
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Mechanism of hsa_circ_0069443 promoting early pregnancy loss through ALKBH5/FN1 axis in trophoblast cells by Bai-xue Li, Mei-yao Wu, Zhi-hui Wang, Dong-mei Zhou, Jian-qi Li, Bing-feng Lu, Xiao-ling Lin, Yang Zhao, Xiu-jie Sheng

“…This study also found that hsa_circ_0069443 can bind to the RNA-binding protein demethylase ALKBH5, affecting the overall m6A level of trophoblast cells, and hsa_circ_0069443 and ALKBH5 can regulate the expression level of FN1, verifying the role of the 0069443/ALKBH5/FN1 axis in trophoblast cells and endometrial stromal cells. …”
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Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti... by Arndt Borkhardt, Nan Qin, Stephen T Keir, Darell D Bigner, Allison Cole, Matthias Wölfl, Viktoria Marquardt, Johanna Theruvath, David Pauck, Daniel Picard, Lena Blümel, Mara Maue, Jasmin Bartl, Ulvi Ahmadov, Maike Langini, Frauke-Dorothee Meyer, Joselyn Cruz-Cruz, Claus M Graef, Till Milde, Olaf Witt, Anat Erdreich-Epstein, Gabriel Leprivier, Ulf Kahlert, Anja Stefanski, Kai Stühler, Julia Hauer, Thomas Beez, Christiane B Knobbe-Thomsen, Ute Fischer, Jörg Felsberg, Finn K Hansen, Rajeev Vibhakar, Sujatha Venkatraman, Samuel H Cheshier, Guido Reifenberger, Thomas Kurz, Marc Remke, Siddhartha Mitra

“…Here, we report a cross-entity, epigenetic drug screen to evaluate therapeutic vulnerabilities in MYC amplified MB, which sensitizes them to macrophage-mediated phagocytosis by targeting the CD47-signal regulatory protein α (SIRPα) innate checkpoint pathway.Methods We performed a primary screen including 78 epigenetic inhibitors and a secondary screen including 20 histone deacetylase inhibitors (HDACi) to compare response profiles in atypical teratoid/rhabdoid tumor (AT/RT, n=11), MB (n=14), and glioblastoma (n=14). …”
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Efficacy and safety of SHR8554 on postoperative pain in subjects with moderate to severe acute pain following orthopedic surgery: A multicenter, randomized, double-blind, dose-expl... by Yanhua Zhao, Zhisheng Lu, Xuesong Song, Haihui Xie, Xungang Xiao, Guonian Wang, Qi Zhou, Qingmei Zhang, Liang Liu, Zhijian Lan, Ning Bai, Haiyan Wang, Zhihao Pan, Liang Dong, Xianzhong Lin, Gang Chen, Qinghui Wang, Jiangtao Dong, Jia Deng, Yongshan Nan, Jiaqiang Zhang, Xiaohua Zhou, Yanjuan Huang, Yongquan Chen, Huanzhong He, Ye Yang, Dengpan Yao, Jintai Jia, Shuan Jin, Yafeng Zhang, Zhonghui Luo, Jiangang Li, Linzhong Zhang, Fang Wang, Juehua Jing, Jiang Zhu, Lin Li, Guangda Wang, Huajing Guo, Qiang Wang, Diansan Su, Weifeng Yu, Xiyao Gu

“…Biased µ-opioid receptor (MOR) agonists enhance pain relief by selectively activating G protein-coupled receptor signaling and minimizing β-arrestin-2 activation, resulting in fewer side effects. …”
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Changes in laboratory value improvement and mortality rates over the course of the pandemic: an international retrospective cohort study of hospitalised patients infected with SARS... by Ramakanth Kavuluru, Xuan Wang, Paul Avillach, Florence Bourgeois, Kee Yuan Ngiam, Gabriel A Brat, Isaac Kohane, Tianxi Cai, Yuk-Lam Ho, Yuan Luo, Harrison G Zhang, Kelly Cho, Vincent Benoit, Antoine Neuraz, Chuan Hong, Sehi L'Yi, Griffin Weber, Bryce W Q Tan, Alba Gutiérrez-Sacristán, Clara-Lea Bonzel, Nathan P Palmer, Alberto Malovini, Valentina Tibollo, Meghan R Hutch, Molei Liu, Riccardo Bellazzi, Luca Chiovato, Fernando J Sanz Vidorreta, Trang T Le, William Yuan, Bertrand Moal, Michele Morris, David A Hanauer, Sarah Maidlow, Kavishwar Wagholikar, Shawn Murphy, Hossein Estiri, Adeline Makoudjou, Patric Tippmann, Jeffery Klann, Robert W Follett, Nils Gehlenborg, Gilbert S Omenn, Zongqi Xia, Arianna Dagliati, Shyam Visweswaran, Lav P Patel, Danielle L Mowery, Emily R Schriver, Malarkodi Jebathilagam Samayamuthu, Sara Lozano-Zahonero, Daniela Zöller, Amelia L M Tan, Byorn W L Tan, John H Holmes, Petra Schubert, Brett K. Beaulieu-Jones, Miguel Pedrera-Jiménez, Noelia García-Barrio, Pablo Serrano-Balazote, Andrew South

“…The average C reactive protein rate of change was –4.72 mg/dL vs –4.14 mg/dL per day (p=0.05). …”
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Presenting the local model of increasing the market share in Iran's dairy industry with a qualitative approach by leila taheri fard, farzad asayesh, alireza rousta

“…Milk and its products are of special importance in human life due to their extraordinary nutritional value, and they always provide part of the body's needs in the human food pyramid, including protein and some vitamins and especially calcium, but they do not have substitute products; therefore, in terms of examining alternative goods, the circle of investigation should be limited to all types of dairy products (Mousavinejad & Khoramnia, 2021). …”
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Proteomics study of Mongolian medicine warm acupuncture in the treatment of p-chlorophenylalanine-induced insomnia in rats by Yanan Xu, Jianxun Wen, Wenyan Han, Gula A, Xiulan Su

“…In this study, 6383 proteins were identified, including 45 proteins with increased expression and 31 proteins with decreased expression in the insomnia model group (M) compared to the control group (C); 101 proteins showed an increase and 48 proteins showed a decrease between the warm acupuncture group (W) and C; 26 proteins showed an increase and 22 proteins showed a decrease between W and M. …”
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Polarity Determinants in Dendritic Spine Development and Plasticity by Huaye Zhang

“…Interestingly, some polarity proteins also utilize neuronal-specific mechanisms. …”
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