Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis by Junxian Hu, Junxian Hu, Yibo Zhu, Yibo Zhu, Zeyu Pang, Zeyu Pang, Xiaoxiao Li, Xiaoxiao Li, Huilin Zhang, Huilin Zhang, Xiangwei Li, Xiangwei Li, Yongjian Gao, Yongjian Gao, Yiyang Wang, Yiyang Wang, Pei Li, Pei Li, Qiang Zhou, Qiang Zhou

“…Calreticulin (CRT), an endoplasmic reticulum retention protein, performs various physiological functions, including the regulation of intracellular calcium levels. …”
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Cell-free DNA: a metabolic byproduct with diagnostic and prognostic potential in rheumatic disorders by Fancheng Liu, Fancheng Liu, Yazhen Su, Yazhen Su, Xinling Liu, Li Zhao, Li Zhao, Zewen Wu, Zewen Wu, Yang Liu, Yang Liu, Liyun Zhang, Liyun Zhang

“…Additionally, several trials have demonstrated a correlation between cfDNA concentrations and the activity of rheumatic diseases, indicating the potential of cfDNA, a novel marker for inflammation, in conjunction with C-creative protein (CRP), Erythrocyte Sedimentation Rate (ESR) to monitor disease activity in rheumatic conditions. …”
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Biosynthesis and activity of Zn-MnO nanocomposite in vitro with molecular docking studies against multidrug resistance bacteria and inflammatory activators by Samy Selim, Tarek M. Abdelghany, Mohammed S. Almuhayawi, Mohammed K. Nagshabandi, Muyassar K. Tarabulsi, Mohammed Yagoub Mohammed Elamir, Asmaa A. Alharbi, Soad K. Al Jaouni

“…MnO-NPs and ZnO-NPs demonstrated inhibitory activity against the crystal structure of putative minohydrolase (PDB ID: 4EWT), methicillin acyl-penicillin binding protein 2a structure (PDB ID: 1MWU) and K2U bound crystal structure of class II peptide deformylase from MRSA (PDB ID: 6JFQ). …”
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Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats by Axelle Amen, Randy Yoo, Amanda Fabra-García, Judith Bolscher, William JR Stone, Isabelle Bally, Sebastián Dergan-Dylon, Iga Kucharska, Roos M de Jong, Marloes de Bruijni, Teun Bousema, C Richter King, Randall S MacGill, Robert W Sauerwein, Jean-Philippe Julien, Pascal Poignard, Matthijs M Jore

“…Thus, this mode of recognition of Pf proteins, previously described only for Pf circumsporozoite protein (PfCSP), extends to other repeats expressed across various stages. …”
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Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet by Xinyan Zhi, Yixin Gu, Wenli Zhao, Shichao Xie, Yangguang Bao, Sofia Morais, Dong Li, Maolong He, Min Jin, Qicun Zhou

“…A positive control diet (PC) was formulated in which fish meal content was 45 %, and in the negative control diet (NC) fish meal was reduced to 27 % and substituted with soybean protein concentrate. A third experimental group consisted of the NC diet supplemented with 0.15 % PE (NC + PE). …”
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Rosiglitazone, a Ligand to PPARγ, Improves Blood Pressure and Vascular Function through Renin-Angiotensin System Regulation by María Sánchez-Aguilar, Luz Ibarra-Lara, Leonardo Del Valle-Mondragón, María Esther Rubio-Ruiz, Alicia G. Aguilar-Navarro, Absalom Zamorano-Carrillo, Margarita del Carmen Ramírez-Ortega, Gustavo Pastelín-Hernández, Alicia Sánchez-Mendoza

“…We conclude that RGZ lowers blood pressure values by increasing the expression of RAS axis proteins ACE2 and AT2R, decreasing the levels of AngII and increasing levels of Ang-(1-7) in a PPARγ-dependent manner. …”
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Long-term trends and patterns in ultra-processed food consumption among Korean adults from 1998 to 2022 by Hyeseung Lee, Yesol Yim, Yerin Cho, Jiyeon Oh, Soeun Kim, Yejun Son, Hyesu Jo, Seohyun Hong, Masoud Rahmati, Hanseul Cho, Jiyoung Hwang, Dong Keon Yon

“…In 2020–2022, ultra-processed foods had a higher proportion of calories from carbohydrates (62.12% vs. 58.81%) and fats (28.22% vs. 22.31%) but a lower proportion from protein (10.32% vs. 17.26%) compared to non-ultra-processed foods. …”
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A Native LH1–RC–HiPIP Supercomplex from an Extremophilic Phototroph by Kazutoshi Tani, Ryo Kanno, Kenji V. P. Nagashima, Mai Kawakami, Naho Hiwatashi, Kazuna Nakata, Sakiko Nagashima, Kazuhito Inoue, Shinichi Takaichi, Endang R. Purba, Malgorzata Hall, Long-Jiang Yu, Michael T. Madigan, Akira Mizoguchi, Bruno M. Humbel, Yukihiro Kimura, Zheng-Yu Wang-Otomo

“…Despite overall highly negative surface-charge distributions for both the cytochrome subunit and HiPIP, the interface between the two proteins was relatively uncharged and neutral, forming a pathway for electron tunneling. …”
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Multidimensional features of sporadic Creutzfeldt-Jakob disease in the elderly: a case report and systematic review by Jiangfeng Liao, Jiangfeng Liao, Wenming Hu, Wenming Hu, Shiheng Chen, Chunyu Huang, Chunyu Huang, Senwei Dong, Senwei Dong, Wanjin Chen, Wanjin Chen, Xiaochun Chen, Longfei Chen, Longfei Chen

“…The sensitive cerebrospinal fluid biomarkers were total tau (83.3%), 14-3-3 protein (75.0%), and PrP RT-QuIC (75.0%). Neuropathological profiles in the cerebral cortex revealed vacuolar spongiosis, neuronal loss, gliosis, and aging-related markers, with synaptic deposit as the commonest PrP pattern (60.0%). …”
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An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing by Jacob W. Fleming, Molly C. McCloskey, Kevin Gray, David R. Nash, Vincent Leung, Christos Michas, Shawn M. Luttrell, Christopher Cavanaugh, Julie Mathieu, Shawn Mcquire, Mark Bothwell, David L. Mack, Nicholas A. Geisse, Alec S.T. Smith

“…Histological analysis revealed characteristic punctate acetylcholine receptor staining co-localized with the presynaptic marker synaptic vesicle protein-2. Dose-response studies using botulinum neurotoxin showed loss of function in a dose- and time-dependent manner (EC50 − 0.11 ± 0.015 µg). …”
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Elevated Serum Extracellular Vesicle‐Packaged SPARC in Hypertension: A Cross‐Sectional Study in a Middle‐Aged and Elderly Population by Xueying Chen, Han Guo, Xinwei Li, Yang Liu, Xinxin Li, Zhengshuo Cui, Huijuan Ma, Jianxun He, Zhechun Zeng, Huina Zhang

“…However, studies on the relevance of EV cargos to hypertension are limited. Secreted protein acidic and rich in cysteine (SPARC) is involved in many metabolic diseases and endothelial dysfunction pathological processes. …”
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Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy by Coraline Radermecker, Pascale Hubert, Patrick Roncarati, Stephanie Demoulin, Charlotte Pilard, Marie Ancion, Celia Reynders, Thomas Lerho, Diane Bruyere, Alizee Lebeau, Margot Meunier, Marie-Julie Nokin, Elodie Hendrick, Olivier Peulen, Philippe Delvenne, Michael Herfs

“…Background High-mobility group box 1 (HMGB1) is a multifunctional redox-sensitive protein involved in various intracellular (eg, chromatin remodeling, transcription, autophagy) and extracellular (inflammation, autoimmunity) processes. …”
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Elevated D-dimer on admission may predict poor prognosis in childhood influenza associated encephalopathy by Huizhen Wang, Lingkong Zeng, Xingfeng Cheng, Hui Li, Hong Zhang, Yuanmei Shi, Yong Zhang, Changjian Li

“…In the univariate analysis, the poor prognosis group compared with the clinically cured group showed a significant increase in the number of days of hospitalization (3.0 vs. 9.5 days, P < 0.001), high-sensitivity C-reactive protein (6.80 vs. 1.88 mg/L, P = 0.003), interleukin-6 (20.26 vs. 8.24 pg/mL, P = 0.001), interleukin-10 (11.75 vs. 4.72 pg/mL, P = 0.003), alanine aminotransferase (104.0 vs. 20.0 U/L, P = 0.011), aspartate azelotransferase (186.5 vs. 37.0 U/L, P = 0.003), serum albumin (37.99 vs. 40.76 g/L, P = 0.042), prothrombin time (13.2 vs. 11.4 s, P = 0.017), D-dimer (4.34 vs. 0.44 mg/L FEU, P < 0.001), peripheral blood CD19 B-cell count (35.11 vs. 32.75 cells/µL, P = 0.018), and cerebrospinal fluid chloride (126.82 vs. 125.50 mmol/L, P = 0.027) were statistically different in the above 11 indicators. …”
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Serum and tear autoantibodies from NOD and NOR mice as potential diagnostic indicators of local and systemic inflammation in Sjögren’s disease by Shruti Singh Kakan, Sara Abdelhamid, Sara Abdelhamid, Yaping Ju, Yaping Ju, J. Andrew MacKay, J. Andrew MacKay, J. Andrew MacKay, Maria C. Edman, Indu Raman, Chengsong Zhu, Prithvi Raj, Sarah F. Hamm-Alvarez, Sarah F. Hamm-Alvarez

“…BackgroundSjögren’s Disease (SjD) is an autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands (LG). The LG produces the protein-rich aqueous component of tears, and SjD-associated autoimmune dacryoadenitis (AD) may thus alter tear autoantibody composition.MethodsThe presence of tertiary lymphoid structures (TLS) in LG from two murine models of SjD-associated AD, male non-obese diabetic (NOD) and male non-obese insulitis resistant (NOR) mice, were evaluated using immunofluorescence. …”
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IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice by Tingwenyi Hu, Xiangrong Tang, Tiechao Ruan, Shunhua Long, Guicen Liu, Jing Ma, Xueqi Li, Ruoxuan Zhang, Guoning Huang, Ying Shen, Tingting Lin

“…Computer-aided sperm analysis, Papanicolaou staining, and electron microscopy were applied to evaluate the motility, morphology, and ultrastructure of spermatozoa, respectively. Protein mass spectrometry, western blotting, and bioinformatic analyses were performed to identify critical components of mammalian RS1 to model its structure and explore the pathological mechanism of IQUB deficiency. …”
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Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport by Mengmeng Wang, Min Yu, Amel Thanina Amrouche, Fan Jie, Shengyang Ji, Baiyi Lu

“…Ezetimibe could significantly inhibit the transport of 7-ketophytosterols (P < 0.05), which means that their transport depends on niemann-pick c1-like 1 (NPC1L1) protein. The transport of cholesterol-d7 was significantly inhibited by 7-ketophytosterols (P < 0.05). …”
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The efficacy of neoadjuvant immunotherapy and lymphocyte subset predictors in locally advanced esophageal squamous cell carcinoma: A retrospective study by Ruotong Wang, Shaodi Wen, Xiaoyue Du, Jingwei Xia, Bowen Hu, Yihan Zhang, Guoren Zhou, Feng Jiang, Xiaomin Lu, Miaolin Zhu, Xinyu Xu, Bo Shen

“…Abstract Background Despite the recognized therapeutic potential of programmed cell death protein 1/programmed death‐ligand 1 (PD‐1/PD‐L1) inhibitors in advanced esophageal squamous cell carcinoma (ESCC), their role in neoadjuvant therapy and reliable efficacy biomarkers remain elusive. …”
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Effect of Repetitive Transcranial Magnetic Stimulation on Patients with Post-Stroke Cognitive Impairment by Zheyi YU, Weiming ZHANG

“…ELISA method was used to observe the changes of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), interleukin-6(IL-6) and high-sensitivity C-reactive protein (hs-CRP) in the two groups after treatment.Results:There were no significant differences in the scores of MoCA, including orientation (ORT), visual space and executive function (EF), naming (NAM), memory (MEM), attention (ATT), language (LANG) and abstract ability (ABS) before treatment between the two groups (<italic>P</italic>&gt; 0.05). …”
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Cardiac repair using regenerating neonatal heart tissue-derived extracellular vesicles by Hanjing Li, Yining Liu, Yuqing Lin, Sijia Li, Chenlu Liu, Ao Cai, Wei Li, Wanyu Zhang, Xinlu Gao, Zhongyu Ren, Haoyu Ji, Yang Yu, Xiuxiu Wang, Wenya Ma, Ning Wang, Dan Zhao, Tianlong Li, Yu Liu, Benzhi Cai

“…Proteomic analysis reveals the expression of Wdr75 protein, a regulator of p53, is higher in AR-Neo-EVs than in Neo-EVs. …”
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Decrease in phase angle after Roux-en-Y gastric bypass is related to increase of anti-inflammatory status by Amanda Cristina Araújo Gomes, Amanda Machado Fiorotti, Amanda Motta de Bortoli, Beatriz Bobbio de Brito, João Arthur Souza Fiorido, Luciane Bresciani Salaroli, Andressa Bolsoni Lopes, Fabiano Kenji Haraguchi

“…Assessments included serum levels of alpha-1-acid glycoprotein (AGP), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), adiponectin, and resistin, as well as anthropometric data and body composition. …”
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