Targeting UGT2B15 and NR1H4 interaction: a novel therapeutic strategy for polycystic ovary syndrome using naftopidil enantiomers by Xiufen Zheng, Zikai Chen, Miao Liang, Liting Zhou, Miaoru Wang, Silin Zhang, Shuyun Zhang, Lei Ma, Wei Yi, Xiawen Liu

“…(R)-NAF and (S)-NAF disrupted NR1H4 binding to the UGT2B15 promoter without affecting its protein levels, indicating direct interference with its regulation. …”
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Comprehensive evaluation of the nutritional value and contaminants of alfalfa (Medicago sativa L.) in China by Xiaoyan Zhu, Xiaoyan Zhu, Xiaoyan Zhu, Wenxue Chen, Mengyao Li, Boshuai Liu, Boshuai Liu, Boshuai Liu, Shumin Zhao, Menglin Hu, Jiajing Li, Defeng Li, Defeng Li, Defeng Li, Yinghua Shi, Yinghua Shi, Yinghua Shi, Hao Sun, Hao Sun, Hao Sun, Chengzhang Wang, Chengzhang Wang, Chengzhang Wang

“…Nutritional value analysis and contamination evaluation are essential for sustainable utilisation to ensure the quality and safety of alfalfa.AimWe aimed to evaluate the nutritional value and safety of alfalfa in five major regions in China—the Northeast Plain (NP), Inner Mongolia Plateau (IMP), Northwest Desert Oasis (NO), Loess Plateau (LP), and Huanghuaihai Plain (HP).MethodsNinety-five samples representing 44 large-scale pratacultural companies were collected and analysed.ResultsThe average nutritional values of alfalfa hay were 16.43% crude protein (CP), 44.01% neutral detergent fibre (NDF), 33.22% acid detergent fibre (ADF), 10.78% crude ash (Ash), and 91.90% dry matter (DM), which were better in the NO and IMP areas; in particular, the α-linolenic acid value of alfalfa was the highest in the IMP area (p < 0.05). …”
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Multiomics unravels the complexity of male obesity: a prospective observational study by Georgios E. Papadakis, Lucie Favre, Yassine Zouaghi, Nathalie Vionnet, Nicolas J. Niederländer, Michela Adamo, James S. Acierno, Dassine Berdous, Alexia Boizot, Jenny Meylan, Julijana Ivanisevic, Emmanuelle Paccou, Hector Gallart-Ayala, Tim Reyns, Elise Van Caeneghem, Bruno Lapauw, Jérôme Pasquier, Yasser Aleman, Styliani Mantziari, Olivier Salamin, Raul Nicoli, Tiia Kuuranne, Tom Fiers, Patric Hagmann, Federico Santoni, Andrea Messina, Nelly Pitteloud

“…The upregulated genes were involved mainly in inflammation, mitochondrial function, and protein translation. Integration of gene expression and clinical data revealed high FGF21 and low cortisol levels as the top markers correlated with the transcriptomic signature of metabolic risk. …”
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The microbiota-derived bile acid taurodeoxycholic acid improves hepatic cholesterol levels in mice with cancer cachexia by Morgane M. Thibaut, Martin Roumain, Edwige Piron, Justine Gillard, Axelle Loriot, Audrey M. Neyrinck, Julie Rodriguez, Isabelle Massart, Jean-Paul Thissen, Joshua R. Huot, Fabrizio Pin, Andrea Bonetto, Nathalie M. Delzenne, Giulio G. Muccioli, Laure B. Bindels

“…In vitro, TDCA prevented myotube atrophy, whereas in vivo hepatic whole transcriptome analysis revealed that TDCA administration to cachectic mice improved the unfolded protein response and cholesterol homeostasis pathways. …”
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Ginkgetin Alleviates Inflammation and Senescence by Targeting STING by Yadan Liu, Jialin Ye, Zisheng Fan, Xiaolong Wu, Yinghui Zhang, Ruirui Yang, Bing Jiang, Yajie Wang, Min Wu, Jingyi Zhou, Jingyi Meng, Zhiming Ge, Guizhen Zhou, Yuan Zhu, Yichuan Xiao, Mingyue Zheng, Sulin Zhang

“…Subsequent cell biological and biophysical data confirmed that Ginkgetin directly binds to the carboxy‐terminal domain of STING protein, thereby inhibiting STING activation and signal transduction. …”
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Serum ALT activity and its isoenzymes as potential biomarkers for diagnosis of Sarcopenia in older adults: a retrospective, cross-sectional study by Jiangping Zeng, Nannan Li, Jiaying Ge, Huihui Ma, Siqi Sun, Yujie Jing, Chunhua Qian, Ran Cui, Shen Qu, Hui Sheng

“…Abstract Background Alanine aminotransferase (ALT) is an enzyme crucial for energy and protein metabolism in muscle cells. Despite this, its association with sarcopenia remains inadequately explored. …”
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DLBWE-Cys: a deep-learning-based tool for identifying cysteine S-carboxyethylation sites using binary-weight encoding by Zhengtao Luo, Zhengtao Luo, Zhengtao Luo, Qingyong Wang, Qingyong Wang, Qingyong Wang, Yingchun Xia, Yingchun Xia, Yingchun Xia, Xiaolei Zhu, Xiaolei Zhu, Xiaolei Zhu, Shuai Yang, Shuai Yang, Shuai Yang, Zhaochun Xu, Zhaochun Xu, Lichuan Gu, Lichuan Gu, Lichuan Gu

“…Thus, this model paves the way for predicting cysteine S-carboxyethylation modification sites in protein sequences. The source code of DLBWE-Cys and experiments data are available at: https://github.com/ztLuo-bioinfo/DLBWE-Cys.…”
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Identification of Lauric Acid as a Potent Sodium Channel NaV1.5 Blocker from Compound Chinese Medicine Wenxin Keli by Xie W, Gao J, Liang Y, Huang C, Zhang B, Chen X, Yao X, Nan G, Wu H, Wang Y, Wu L, Wang T, Zhu Y

“…Molecular docking and molecular dynamics simulation suggested that LA binds to the NaV 1.5 protein, with a significant binding affinity forming interactions with functionally essential residues and blocks the inward flow of Na+. …”
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Determination and prediction of the apparent and standardized ileal amino acid digestibility in soybean meal fed to the pullets by Sumei Cao, Yuxin Yang, Guangtian Ma, Jing Wang, Kai Qiu, Zhigang Song, Shugeng Wu, Guanghai Qi, Haijun Zhang

“…The AID of AA was correlated with the dry matter (DM), gross energy (GE), crude protein (CP), EE, Ash, and reactive Lys (rLys) (P < 0.05), and SID of AA was correlated with the GE, CP, EE, CF, neutral detergent fiber (NDF) and rLys (P < 0.05). …”
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Irisin regulates oxidative stress and mitochondrial dysfunction through the UCP2-AMPK pathway in prion diseases by Pei Wen, Zhixin Sun, Dongming Yang, Jie Li, Zhiping Li, Mengyang Zhao, DongDong Wang, Fengting Gou, Jingjing Wang, Yuexin Dai, Deming Zhao, Lifeng Yang

“…Abstract Prion diseases are a group of fatal neurodegenerative disorders characterized by the abnormal folding of cellular prion proteins into pathogenic forms. The development of these diseases is intricately linked to oxidative stress and mitochondrial dysfunction. …”
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Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) by Muslim Qadir, Muslim Qadir, Muslim Qadir, Xinyi Lin, Farhan Nabi, Kishore Kumar Ashok, Xue-Rong Zhou, Qingbin Sun, Peiman Shi, Xinfa Wang, Jiaqin Shi, Hanzhong Wang

“…Transcriptomic analysis identified 7689 differentially expressed genes (DEGs) between the two pools, of which nearly half were enriched into functional categories of reported genes regulating ONPO, including protein, RNA, signalling, miscellaneous, development, hormone metabolism, and tetrapyrrole synthesis. …”
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Sox6 and ALDH1A1 Truncation by Asparagine Endopeptidase Defines Selective Neuronal Vulnerability in Parkinson's Disease by Shuke Nie, Bowei Li, Mengmeng Wang, Zijun Chen, Jiayan Ren, Zixuan Li, Xinli Xu, Zhengjiang Qian, Zhongyun Xie, Jianxin Han, Zhentao Zhang, Zhaohui Zhang, Yingjie Zhu, Zuxin Chen, Xifei Yang, Keqiang Ye

“…Here, it is shown that Legumain, also known as asparagine endopeptidase (AEP), is activated in a subgroup of SRY‐box transcription factor 6 /Aldehyde dehydrogenase 1 family member A1, (Sox6+/ALDH1A1+) neurons in the ventral tier of the SNpc and cleaves Sox6 and ALDH1A1, leading to repression of Special AT‐rich sequence binding protein 1 (Satb1) that is a dimeric/tetrameric transcription factor specifically binding to AT‐rich DNA sequences, and toxic dopamine metabolite accumulation. …”
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Ginsenoside Rg1 alleviates chronic testicular damage caused by cranial irradiation through the SCF/PI3K/Akt/mTOR pathway in mice by Qiuhua Zhou, Yiquan Ou, Xiangsheng Tian, Yujun Ning, Yuwei Mao, Weichao Zhao, Dingxin Long

“…Additionally, quantitative PCR and Western blot showed that Rg1 downregulated SCF, p-PI3K, p-Akt, and mTOR protein expressions in irradiated mice. Conclusions: Ginsenoside Rg1 potentially alleviate chronic testicular structural and functional damage by inhibiting germ cell apoptosis through the modulation of the HPG axis and the PI3K/Akt/mTOR pathway, suggesting that it is a potential therapeutic agent for reproductive disorders induced by cranial irradiation.…”
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S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling by Liuyuan Xia, Xin Guo, Dong Lu, Yixin Jiang, Xiaohui Liang, Yiwen Shen, Jiayi Lin, Lijun Zhang, Hongzhuan Chen, Jinmei Jin, Xin Luan, Weidong Zhang

“…Recent studies have highlighted the importance of calcium signaling in enhancing interactions between tumor cells and the surrounding stroma, with the S100 family of proteins serving as important regulators. While the roles of some S100 proteins have been extensively studied, others, such as S100A13, remain less well understood. …”
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Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats by Amaka Cecilia Agbara, Ejike Daniel Eze, Christian Emeka Offor, Adam Moyosore Afodun, Ezebuilo Ugbala Ekpono, Peter Chinedu Agu, Chinyere Aloke, Nkeiru Nwaamaka Ezeani, Emmanuel Orire Ikuomola, Ekom Monday Etukudo, Ilemobayo Victor Fasogbon, Angela Mumbua Musyoka, Patrick Maduabuchi Aja

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Ion homeostasis and coordinated salt tolerance mechanisms in a barley (Hordeum vulgare L.)doubled haploid line by Hongwei Xu, Hui Chen, Nigel G. Halford, RugenXu, Ting He, Bangwei Yang, Longhua Zhou, HuiminGuo, ChenghongLiu

“…Furthermore, the activities of ascorbate peroxidase, superoxide dismutase, and peroxidase, along with the levels of proline, betaine, malondialdehyde, and soluble protein, were correlated with ion efflux and played a vital role in the response of DH20 to salt stress. …”
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Implication of Nonalcoholic Fatty Liver Disease, Metabolic Syndrome, and Subclinical Inflammation on Mild Renal Insufficiency by Ga Eun Nam, Soon Young Hwang, Hye Soo Chung, Ju Hee Choi, Hyun Jung Lee, Nam Hoon Kim, Hye Jin Yoo, Ji-A Seo, Sin Gon Kim, Nan Hee Kim, Sei Hyun Baik, Kyung Mook Choi

“…In partial correlation analysis, several components of MetS and liver aminotransferase levels, but not high-sensitivity C-reactive protein (hsCRP), were associated with eGFR. Multivariate logistic regression analysis demonstrated the independent association of NAFLD (P=0.034) and MetS (P=0.018) with mild renal insufficiency, but not elevated hsCRP (P=0.885). …”
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Nucleated red blood cells for characterization of systemic inflammatory response syndrome in dogs by ARom Cho, Hyeona Bae, Youngju Kim, Yeseul Jeon, Rankyung Jung, Minji Kim, Minjeong Kang, Sumin Cha, Kyu‐Woan Cho, Dong‐In Jung, Jinho Park, DoHyeon Yu

“…Results The median WBC (17.63, interquartile range [IQR]: 11.72‐20.24 × 109/L), neutrophils (12.28, IQR: 7.17‐16.88 × 109/L), band neutrophils (1288.5, IQR: 252.5‐2575 cells/μL), serum IL‐6 (731.80, IQR: 299.79‐5522.05 pg/mL), and plasma C‐reactive protein (4.10, IQR: 1.00‐8.58 mg/L) were significantly higher in nRBC‐positive dogs than negative dogs (11.27, IQR: 7.63‐15.13 × 109/L; 7.57, IQR: 4.96‐11.71 × 109/L; 62.5, IQR: 0‐350.25 cells/μL; 232.30, IQR: 99.33‐447.01 pg/mL; 0.40, IQR: 0.10‐3.00 mg/L, respectively; P < .05). …”
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A c-type lectin HcLec1 with dual function of immunology and mineralization from the freshwater oyster (Hyriopsis cumingii Lea) by Xiaofeng Chen, Xiaoya Shen, Shijun Liu, Wenjuan Li, Wenjuan Li, He Wang, Jiale Li, Jiale Li, Zhiyi Bai, Zhiyi Bai

“…The recombinant HcLec1 protein demonstrated binding affinity to LPS and PGN, a robust ability to agglutinate Escherichia coli, Staphylococcus aureus, Aeromonas veronii, and Aeromonas hydrophila, and significantly inhibited bacterial growth (P &lt; 0.05). …”
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Urinary biomarkers for diagnosing acute kidney injury in sepsis in the emergency department by Sumin Baek, Inwon Park, Seonghye Kim, Young Woo Um, Hee Eun Kim, Kyunghoon Lee, Jae Hyuk Lee, You Hwan Jo

“…Initial urine samples were collected, and urinary biomarkers (dickkopf-3, soluble triggering receptor expressed on myeloid cells-1, kidney injury molecule-1, neutrophil gelatinase-associated lipocalin (NGAL), and tissue inhibitor of metalloproteinases-2 (TIMP-2), and insulin-like growth factor binding protein-7 (IGFBP-7), and TIMP-2 × IGFBP-7) were analyzed using an enzyme-linked immunosorbent assay kit. …”
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