Insights into mungbean defense response to Cercospora leaf spot based on transcriptome analysis by Sukanya Inthaisong, Pakpoom Boonchuen, Tana Jaichopsanthia, Pongpan Songwattana, Apinya Khairum, Witsarut Chueakhunthod, Akkawat Tharapreuksapong, Panlada Tittabutr, Neung Teaumroong, Piyada Alisha Tantasawat

“…In addition, the qRT-PCR was used to analyze the expression pattern of 22 candidate DEGs belonging to pathogenesis related (PR) proteins, resistance (R) proteins, transcription factors, hypersensitive response (HR), and the essential genes involved in cell wall activity during CLS-infected V. radiata. …”
Get full text

Reproductive performance of female largemouth bass (Micropterus salmoides) broodstock was enhanced by dietary small peptides by Jie Wei, Mingwei Tao, Jiaming Huang, Hangxian Zhou, Qiyou Xu

“…The mRNA expression levels of vgr (1.90 ± 0.29), insulin-like growth factor-1 (1.53 ± 0.67), steroidogenic acute regulatory protein (2.59 ± 0.78), fshr (1.97 ± 0.35) and cyp17a (2.78 ± 1.32) in gonad were up-regulated in the HS group (P < 0.05). …”
Get full text

Comparison of the mid-infrared spectra and prediction equations developed from morning and evening milk samples from twice-a-day milked dairy cows by M. Frizzarin, E. Hayes, A. Casa, D.P. Berry

“…ABSTRACT: Mid-infrared spectroscopy is a technology used globally for quantifying the concentration of fat, protein, lactose, and other constituents in the milk samples of both individual animals and bulk tank milk. …”
Get full text

Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosis by Joan Chang, Adam Pickard, Jeremy A Herrera, Sarah O'Keefe, Richa Garva, Matthew Hartshorn, Anna Hoyle, Lewis Dingle, John Knox, Thomas A Jowitt, Madeleine Coy, Jason Wong, Adam Reid, Yinhui Lu, Cédric Zeltz, Rajamiyer V Venkateswaran, Patrick T Caswell, Stephen High, Donald Gullberg, Karl E Kadler

“…Endogenous collagen production was not required for fibrillogenesis if exogenous collagen was available, but the circadian-regulated vacuolar protein sorting (VPS) 33b and collagen-binding integrin α11 subunit were crucial to fibrillogenesis. …”
Get full text

Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer by Jeremy L. Pinyon, Georg vonJonquieres, Stephen L. Mow, Amr Al Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H. Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley

“…Delivery of DNAs encoding secreted alkaline phosphatase and Cal/09 influenza virus hemagglutinin antigen generate high systemic circulating recombinant protein levels and antibody titres. The findings support adoption of SCD‐CC‐GET for vaccines and immunotherapies, and extend the utility of this technology to meet the demand for efficient vector‐free, precision, deep tissue delivery of NA therapeutics.…”
Get full text

Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig by Peidong Yang, Tingting Luo, Shuqi Yang, Anjing Zhang, Yuan Tang, Li Chen, Jinyong Wang, Yongju Zhao, Zhining Zhong, Xuemin Li, Ziyin Han, Yupei Zhang, Yue Tang, Jideng Ma, Long Jin, Keren Long, Mingzhou Li, Lu Lu

“…Olfactory receptors (ORs) are members of the transmembrane G protein-coupled receptor superfamily, playing a crucial role in odor recognition, which further mediates crucial biological processes in mammals. …”
Get full text

Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil by Felipe Alves Morais, Alexandre Pereira, Aparecida Santo Pietro Pereira, Marcos Lazaro Moreli, Luís Marcelo Aranha Camargo, Marcello Schiavo Nardi, Cristina Farah Tófoli, Jansen Araujo, Lilia Mara Dutra, Tatiana Lopes Ometto, Renata Hurtado, Fábio Carmona de Jesus Maués, Tiene Zingano Hinke, Sati Jaber Mahmud, Monica Correia Lima, Luiz Tadeu Moraes Figueiredo, Edison Luiz Durigon

“…Using a recombinant nucleocapsid protein of Araraquara virus, we performed ELISA to detect IgG antibodies against hantavirus in human sera. …”
Get full text

CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Organoids could be categorized into the established quasimesenchymal, exocrine-like, and classical subtypes based on KRT81 and CFTR immunoreactivity. CFTR protein expression was confirmed on the transcript level. …”
Get full text

Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1 by Xinxin Li, Jinghua Cui, Zanbo Ding, Ziyan Tian, Yiming Kong, Linghai Li, Yang Liu, Wen Zhao, Xueying Chen, Han Guo, Zhengshuo Cui, Xinwei Li, Jing Yuan, Huina Zhang

“…Notably, overexpression or activation of SIRT1 attenuated the adverse effects and protein changes induced by K.pn EVs on endothelial cells. …”
Get full text

Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model by Tala Curry-Koski, Tala Curry-Koski, Liam P. Curtin, Mitra Esfandiarei, Mitra Esfandiarei, Mitra Esfandiarei, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas

“…Despite these significant clinical observations, investigation into cerebrovascular dysfunction and neuropathology in MFS remains limited.MethodsUsing 6M-control (C57BL/6) and 6M-MFS (Fbn1C1041G/+) and healthy 12M-control male and female mice, we test the hypothesis that abnormal Fbn1 protein expression is associated with altered cerebral microvascular density, BBB permeability, and neuroinflammation in the PCA-perfused hippocampus, all indicative of a pre-mature aging brain phenotype. …”
Get full text

Proximal, Microbiological and Nutritional Characterization in Chinese Potato Flour of the White Variety Colocasia Esculenta for Application in Functional Foods by F. López, L. Arboleda, V. González

“…By obtaining the flour, the proximal percentages are evaluated; moisture 9.01%, ash 2.24 ± 0.15% protein 2.02 ± 0.02% fiber 4.25 ± 0.26% and fat 0.43 ± 0.15%. …”
Get full text

Effects of Hypoxia Stress on Biochemical Indices and Expression of Hypoxia-Related Genes in the Heart of Rainbow Trout (Oncorhynchus mykiss) by Jiuju MA, Jinqiang HUANG, Yongjuan LI, Shenji WU, Lu ZHAO, Yue ZENG

“…Under severe hypoxic stress, lactate dehydrogenase gene (ldh), pyruvate kinase gene (pk), sdh, hif-1α, proline hydroxylase domain protein 2 (egln-1), and von Hippel-Lindau (vhl) expressions were significantly increased at 24 h (P < 0.05) compared to those under moderate hypoxic stress. …”
Get full text

Whole-genome sequencing identifies novel loci for keratoconus and facilitates risk stratification in a Han Chinese population by Yinghao Yao, Xingyong Li, Lan Wu, Jia Zhang, Yuanyuan Gui, Xiangyi Yu, Yang Zhou, Xuefei Li, Xinyu Liu, Shilai Xing, Gang An, Zhenlin Du, Hui Liu, Shasha Li, Xiaoguang Yu, Myopia Associated Genetics Intervention and Consortiums, Hua Chen, Jianzhong Su, Shihao Chen

“…Results The characterization of germline variants entailed correction for population stratification and validation of the East Asian ancestry of the included samples via principal component analysis. For rare protein-truncating variants (PTVs) with minor allele frequency (MAF) < 5%, ZC3H11B emerged as the top prioritized gene, albeit failing to reach the significance threshold. …”
Get full text

mTOR downregulation promotes anti-inflammatory responses via the CCL3-CCR5 axis in hypoxic retinopathy by Tae Kwon Moon, Im Kyeung Kang, Kyoung Jin Lee, Ji Hyun Kim, Hee Jong Kim, A. Reum Han, Ha-Na Woo, Joo Yong Lee, Jun-Sub Choi, Keerang Park, Heuiran Lee

“…AAV2-shmTOR notably decreased mTOR expression in rMC1 cells under hypoxic conditions, as verified by co-staining for mTOR and glial fibrillary acidic protein (GFAP). It effectively interrupted the activation of mTOR signaling triggered by hypoxia. …”
Get full text

Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 by Shen Chen, Kexin Nie, Hongzhan Wang, Yang Gao, Xinyue Jiang, Hao Su, Zhi Wang, Yueheng Tang, Fuer Lu, Hui Dong, Jingbin Li

“…Short-chain fatty acids (SCFAs) in the intestines were measured to determine if WMW’s effects on HSF1 are mediated through SCFAs. Protein expression was assessed using western blot, immunohistochemistry, immunofluorescence and RT-qPCR were employed to detect the mRNA levels. …”
Get full text

Effectiveness and safety of PD-1/L1 inhibitors as first-line therapy for patients with advanced or metastatic urothelial carcinoma who are ineligible for platinum-based chemotherap... by Weiming Liang, Weiming Liang, Zhijing Wang, Zhilong Huang, Yanping Huang, Chunyan Li, Yiwen Liang, Miaoyan Huang, Duo Zhang, Chenchen Li

“…ObjectiveTo evaluate the efficacy and safety of programmed cell death protein 1 or its ligand (PD-1/L1) inhibitors as first-line therapy in advanced or metastatic urothelial carcinoma (mUC) who are ineligible for platinum-based chemotherapy.MethodA systematic search was conducted in four databases (Pubmed, Embase, Web of Science, and the Cochrane Library) to find articles that evaluate the effectiveness of first-line PD-1/L1 inhibitors for mUC, from the establishment of the databases to 22 November 2023. …”
Get full text

Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format by Kalvaityte U, Bagdonas E, Kirdaite G, Kausaite-Minkstimiene A, Uzieliene I, Ramanaviciene A, Popov A, Butkiene G, Karabanovas V, Denkovskij J, Mobasheri A, Bernotiene E

“…The purpose of the present research was to develop sensitive multiplex detection model of QD-based ELISA (QLISA), through the spectroscopic QD-analyte complex measurements in microvolume liquid droplets on a glass microslide.Methods: QLISA was used for the detection of cartilage oligomeric matrix protein (COMP) and human growth hormone (hGH) as model analytes. …”
Get full text

Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function by Annat Raiter, Yael Barhum, Julia Lipovetsky, Chen Menachem, Sharona Elgavish, Shmuel Ruppo, Yehudit Birger, Shai Izraeli, Orna Steinberg-Shemer, Rinat Yerushalmi

“…We recently demonstrated that Galectin-3 (Gal-3) binding protein/Gal-3 complex secreted by TNBC cells induces immunosuppression, through inhibiting CD45 signaling in T cells. …”
Get full text

Belimumab versus telitacicept in sequential treatment after rituximab for refractory lupus nephritis: a real-world multicentre study by Fei Han, Hong Pan, Yi Yang, Jianghua Chen, Jing Xue, Lan Lan, Yiting Chen, Pingping Ren, Yaomin Wang, Xin Lei, Jianhang Xu, Xiaochan Chen, Qiankun Zhang, Junni Wang, Nan Shi, Liangliang Chen, Lie Jin

“…The outcomes of renal response rates were evaluated.Results In this study cohort, 63.3% presented with anti-dsDNA antibody positivity and 79.6% exhibited hypocomplementemia, with a mean Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) Score of 13±6, estimated glomerular filtration rate (eGFR) of 76.2 (30.2, 113.7) mL/min and urinary protein creatinine ratio (uPCR) of 2.45 (0.77, 5.19) g/g. …”
Get full text

SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1 by Linlin Cai, Yunting Xie, Haijing Hu, Chongyang Lv, Liangting Shao, Huhu Wang, Xinglian Xu, Guanghong Zhou

“…Utilizing a combined BALB/c mouse model and HepG2 cell model, we demonstrated that SaaS enhances hepcidin synthesis via the bone morphogenetic protein (BMP)-SMAD pathway, leading to decreased ferroportin 1 level. …”
Get full text