Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production by Guangqiang Wei, Daodian Wang, Daodian Wang, Teng Wang, Gao Wang, Yunmei Chai, Yufang Li, Minhui Mei, Hao Wang, Aixiang Huang

“…PGAAP annotation identified 2,152 protein-encoding genes and 58 rRNAs, 15 tRNAs, and 5 5sRNAs. …”
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Evaluation of the transmission-blocking potential of Plasmodium vivax antigen Pvg37 using transgenic rodent parasites and clinical isolates by Di Zhang, Yan Zhao, Dongyan Liu, Fei Liu, Pengbo Liu, Biying Zhang, Zifang Wu, Wanlapa Roobsoong, Sirasate Bantuchai, Sataporn Thongpoon, Piyarat Sripoorote, Meilian Wang, Liwang Cui, Yaming Cao

“…Transmission-blocking vaccines (TBVs) have shown the potential to inhibit malaria transmission by targeting antigens expressed in sexual-stage parasites. Pbg37, a conserved protein expressed in sexual stages from gametocyte to ookinete in the rodent parasite P. berghei, is a viable target for TBV development.Methods and findingsIn this study, we constructed a transgenic strain, TrPvg37Pb, expressing Pvg37 using the P. berghei ΔPbg37 strain. …”
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Fatty acid synthase inhibition improves hypertension-induced erectile dysfunction by suppressing oxidative stress and NLRP3 inflammasome-dependent pyroptosis through activating the... by Jiaochen Luan, Mengchi Yu, Qi Gu, Xuan Zhou, Yunqiang Shao, Tong Chen, Jiayi Zhang, Zheng Zhu, Ninghong Song, Jie Yang

“…After five weeks of treatment, the erectile function of the rats was assessed, and penile tissues were harvested for further analysis. Molecular and protein expression were assessed using Western blotting, qRT-PCR, immunofluorescence staining, and immunohistochemistry.ResultsThe SHR exhibited ED, indicated by reduced maximum ICP/MAP ratios. …”
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Development of Primer Panels for Whole-Genome Amplification and Sequencing of Human Seasonal Coronaviruses: hCoV-OC43, hCoV-HKU1, hCoV-229E, and hCoV-NL63 by Tamila Musaeva, Artem Fadeev, Maria Pisareva, Veronika Eder, Andrey Ksenafontov, Margarita Korzhanova, Valery Tsvetkov, Alexander Perederiy, Irina Kiseleva, Daria Danilenko, Dmitry Lioznov, Andrey Komissarov

“…A detailed phylogenetic analysis of the sequenced genomes was conducted, highlighting the emergence of hCoV-229E subclades 7b.1 and 7b.2, which carry numerous substitutions in the Spike protein. Additionally, we sequenced a historical hCoV-229E isolate collected in the USSR in 1979, the oldest sequenced 229E virus from Eurasia, and demonstrated that it belongs to Genotype 2. …”
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Predictors of Poor Outcome of Anti-MDA5-Associated Rapidly Progressive Interstitial Lung Disease in a Chinese Cohort with Dermatomyositis by Yuhui Li, Yimin Li, Jian Wu, Miao Miao, Xiaojuan Gao, Wenxin Cai, Miao Shao, Xuewu Zhang, Yan Xu, Lu Cong, Jing He, Xiaolin Sun

“…Antimelanoma differentiation-associated protein 5 (anti-MDA5) autoantibody has been reported in dermatomyositis (DM) to be associated with rapidly progressive interstitial lung disease (RP-ILD). …”
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P Wave Duration/P Wave Voltage Ratio Plays a Promising Role in the Prediction of Atrial Fibrillation: A New Player in the Game by E. Karacop, A. Enhos, N. Bakhshaliyev, R. Ozdemir

“…In a univariate regression analysis, age, smoking, C-reactive protein, brain natriuretic peptide, left atrial diameter, left atrial volume index, P wave duration, P wave voltage, and P wave duration/P wave voltage were significantly associated with the development of new-onset AF. …”
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METTL1 mediates PKM m7G modification to regulate CD155 expression and promote immune evasion in colorectal cancer by Fang Wang, Chen Yang, Fang Zheng, Yang Yan, Guifang Li, Yanyan Feng, Hejia Xu, Zilong He, Dongyan Cai, Hairong Sun, Xiaowei Qi, Yong Mao

“…In this study, the effect of METTL1 on RNA m7G levels was evaluated, the effect of METTL1 on PKM mRNA modification was confirmed, the expression level of the PKM2 protein was detected, and the mechanism involved RT‒qPCR, Western blot, RNA stability analysis and RIP analysis. …”
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Quantifying the impact of fermented liquid bio formulations, biofertilizers and organic amendments on horticultural and soil nutrient traits of garden pea ( Pisum sativum L.) by Vandana Thakur, Praveen Sharma, Pradeep Kumar, Payal Sharma, Sartaj Ahmad Bhat, Sunny Sharma, Rehan Azmi, Shivender Thakur

“…., Jeevmurta 20% + Phosphorus solubilizing bacteria 100% + Farmyard manure, and their interaction were found to be best for improving the various horticultural and soil traits i.e. shelling percentage, protein content, effective nodules per plant, available nitrogen and phosphorus content. …”
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Endothelial and neuronal engagement by AAV-BR1 gene therapy alleviates neurological symptoms and lipid deposition in a mouse model of Niemann-Pick type C2 by Charlotte Laurfelt Munch Rasmussen, Signe Frost Frederiksen, Christian Würtz Heegaard, Maj Schneider Thomsen, Eva Hede, Bartosz Laczek, Jakob Körbelin, Daniel Wüstner, Louiza Bohn Thomsen, Markus Schwaninger, Ole N. Jensen, Torben Moos, Annette Burkhart

“…Genetic modification of brain endothelial cells (BECs) is thought to enable secretion of recombinant proteins thereby overcoming the restrictions of the BBB. …”
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Impaired ARID1A expression attenuated the immune response in gastric cancer via histone acetylation by Yu Tang, Ruizhi Zhang, Gan Mao, Chong Li, Yisong Gao, Xuebing Zhou, Wenxiang Nie, Tianyu Song, Suao Liu, Kaixiong Tao, Peng Zhang, Wei Li

“…Real-time PCR was employed for gene expression analysis, while chromatin immunoprecipitation was used to identify transcriptional regulation on target genes. Protein expression and regulation were assessed through various techniques, including Western blot, ELISA, immunohistochemistry, and immunofluorescence. …”
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One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction by Porfirio Estrada-Rojas, Juan Francisco Hernández-Paz, Claudia Alejandra Rodríguez-González, Mónica Elvira Mendoza-Duarte, Santos Adriana Martel-Estrada, Laura Elizabeth Valencia-Gómez, Héctor Camacho-Montes, Imelda Olivas-Armendáriz

“…This synthesis produced four samples with varying BSA concentrations. Due to the lower protein concentration, which provides fewer nucleation sites, sample A (BSA concentration 5 mg ml ^−1 ) showed a particle size distribution over 100 nm and low stability over time. …”
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Инфузия субанестетических доз кетамина в послеоперационном периоде как средство церебропротекции у детей при хирургической коррекции врожденных пороков сердца: проспективное рандом... by Артём Александрович Ивкин, Е. В. Григорьев, Д. Г. Балахнин, А. В. Синицкая

“…С целью установления эффективности церебропротекции использованы сывороточные маркеры повреждения нейроваскулярной единицы: белок S100-β, нейрон-специфическая енолаза (neuron-specific enolase — NSE), глиальный фибриллярный кислый белок (glial fibrillary acidic protein — GFAP), окклюдин, клаудин-1. Забор крови для анализа маркеров проводился в трех контрольных точках: до начала операции, сразу же после завершения искусственного кровообращения и через 16 ч после завершения операции. …”
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Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney disease by Lauren G. Russell, Maria Kolatsi‐Joannou, Laura Wilson, Jennifer C. Chandler, Nuria Perretta Tejedor, Georgie Stagg, Karen L. Price, Christopher J. Rowan, Tessa Crompton, Norman D. Rosenblum, Paul J. D. Winyard, David A. Long

“…In Cpk mice, total GLI3 and GLI3 repressor protein levels were also increased. Reduction of increased Gli3 levels via heterozygous genetic deletion in Cpk mice did not affect cyst formation. …”
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Nutritional properties of Europen eel (Anguilla anguilla) bone peptide-calcium and its apoptosis effect on Caco-2 cells by Hui Teng, Yuewei Qian, Xiaoyun Fan, Hui Cao, Yuting Tian, Lei Chen

“…Additionally, the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell apoptosis. …”
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Tackling the unyielding: testing two novel approaches against NDM-producing Enterobacterales and Pseudomonas aeruginosa isolates collected in India by Yamuna Devi Bakthavatchalam, Bijayini Behera, Anand Shah, Purva Mathur, Raja Ray, Bashir Ahmed Fomda, Kamini Walia, Balaji Veeraraghavan

“…All E. coli isolates harbored four amino acid inserts in their penicillin-binding protein 3. Using the highest susceptible breakpoint among CLSI, FDA, and EUCAST interpretive criteria, cefiderocol susceptibility was 39.3%, ≤80%, and 57%, for NDM ± OXA-48-like-producing E. coli, NDM ± OXA-48-like-producing K. pneumoniae, and NDM-producing P. aeruginosa, respectively. …”
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P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors by Marjory Alana Marcello, Elaine Cristina Morari, Lucas Leite Cunha, Aline Carolina De Nadai Silva, Dirce Maria Carraro, André Lopes Carvalho, Fernando Augusto Soares, José Vassallo, Laura Sterian Ward

“…Besides its major role in cell proliferation, DNA repair, and apoptosis, functional p53 protein is involved in the induction of antitumor cytotoxic-T-cell activity against carcinoma cells. …”
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Carbohydrate ligand engagement with CD11b enhances differentiation of tumor-associated myeloid cells for immunotherapy of solid cancers by Mei Zhang, Veronique Roche, Victor Sandoval, Claire Wolford, Zachary Senders, Julian Anthony Kim, Susan Pereira Ribeiro, Alex Yicheng Huang, Rafick-Pierre Sekaly, Joshua Lyons

“…We have applied peptide microarrays, multiparameter FACS (fluorescence-activated cell analysis) analysis, cellular/molecular immunological technology, advanced microscopic imaging, and transgenic mouse models of solid cancers, to study the interaction between BG34-200 carbohydrate ligand and CD11b protein and the resulting immunological changes in the context of solid cancers, including osteosarcoma, advanced melanoma, and pancreatic ductal adenocarcinoma (PDAC).Results Our results show that BG34-200 can bind directly to the activated CD11b on its I (or A) domain, at previously unreported peptide residues, in a multisite and multivalent manner. …”
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RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia by Tim Kong, Angelo B. A. Laranjeira, Christopher T. Letson, LaYow Yu, Shuyang Lin, Jared S. Fowles, Daniel A. C. Fisher, Sherwin Ng, Wei Yang, Fan He, Minyoung Youn, Kailen Mark, Ana San Jose, Jingxian Liu, Alexander B. Kim, Maggie J. Cox, Mary C. Fulbright, Aarthi Jayanthan, Gerrit Los, Stacey L. Rentschler, Li Ding, Kathleen M. Sakamoto, Sandra E. Dunn, Grant A. Challen, Stephen T. Oh

“…Combining ATAC-Seq, CUT&Tag, RNA-seq, and CyTOF, we demonstrate that targeting of ribosomal protein S6 kinase A1 (RSK1) suppresses NFκB activation and diminishes pro-inflammatory mediators including tumor necrosis factor (TNF) associated with MPN disease severity and transformation. …”
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Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks by Shiyu Wu, Xinrui Liu, Yaxin Liu, Shikai Wang, Wei Peng, Ming Zhang, Binglin Yue, Hui Wang, Jikun Wang, Jincheng Zhong, Fang Sun, Yixi Kangzhu, Jiabo Wang

“…Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, which will affect the growth and development of animals and will bring huge economic benefits when applied to actual production and life. …”
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Preoperative Butyrylcholinesterase Level as an Independent Predictor of Overall Survival in Clear Cell Renal Cell Carcinoma Patients Treated with Nephrectomy by Takuya Koie, Chikara Ohyama, Jotaro Mikami, Hiromichi Iwamura, Naoki Fujita, Tendo Sato, Yuta Kojima, Ken Fukushi, Hayato Yamamoto, Atsushi Imai, Shingo Hatakeyama, Takahiro Yoneyama, Yasuhiro Hashimoto, Masato Kitayama, Kazuyoshi Hirota

“…On univariate analysis, performance status; anemia; hypoalbuminemia; preoperative levels of BChE, corrected calcium, and C-reactive protein; and distant metastasis status were significantly associated with OS. …”
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