Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines by Anton Wellstein, Thomas Seufferlein, Alexander Kleger, Tim Eiseler, Anton Lahusen, Nora Minhöfer, Kim-André Lohse, Christine Blechner, Jessica Lindenmayer, Sabine Windhorst, Yuan-Na Lin

“…The action of TGM2 via interaction with tubulin and the impact of microtubule dynamics and vesicle trafficking were evaluated by protein analyses and live-cell imaging. Correlation analyses via TCGA data complemented the functional studies.Results We identified transglutaminase 2 (TGM2) as a mediator of T cell suppression in PDAC. …”
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On the substrate turnover rate of NBCe1 and AE1 SLC4 transporters: structure-function considerations by Alexander Pushkin, Liyo Kao, Hristina R. Zhekova, Rustam Azimov, Natalia Abuladze, Xuesi M. Shao, D. Peter Tieleman, Ira Kurtz, Ira Kurtz

“…A transport protein’s turnover rate (TOR) is the maximum rate of substrate translocation under saturating conditions. …”
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Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats by Faisal A. Alzahrani, Mohammed A. El-Magd, Ahmed Abdelfattah-Hassan, Ayman A. Saleh, Islam M. Saadeldin, Eman S. El-Shetry, Abdelnaser A. Badawy, Saleh Alkarim

“…CSCs-exosomes also decreased apoptosis (marked by downregulation of Bax and p53 and upregulation of Bcl2, and increased immunostaining of PCNA), increased angiogenetic activity (revealed by upregulation of VEGF), enhanced metastasis and invasiveness (indicated by upregulation of P13K and ERK proteins and their downstream target MMP9 and downregulation of TIMP1), and induced epithelial mesenchymal transition (marked by increased serum and hepatic level of TGFβ1 mRNA and protein). …”
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Predictive modelling and identification of critical variables of mortality risk in COVID-19 patients by Olawande Daramola, Tatenda Duncan Kavu, Maritha J. Kotze, Jeanine L. Marnewick, Oluwafemi A. Sarumi, Boniface Kabaso, Thomas Moser, Karl Stroetmann, Isaac Fwemba, Fisayo Daramola, Martha Nyirenda, Susan J. van Rensburg, Peter S. Nyasulu

“…Also, Age at admission, Pf ratio (PaO2/FiO2 ratio), troponin T (TropT), ferritin, ventilation, C-reactive protein (CRP), and symptoms of acute respiratory distress syndrome (ARDS) were associated with the severity and fatality of COVID-19 cases. …”
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Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…Measures of cognitive function, neuronal damage, white matter lesion (WML), the fibrous profiles of pericytes and ECM protein were investigated to assess the effect of EDB. …”
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Influence of Chronic Alcohol Use on Osteoblastic Differentiation of Bone Marrow Cells, Bone Properties, and Hepatic and Renal Morphology of Rats by Michelle Cardoso de Sousa, Mariana Raquel da Cruz Vegian, Matheus Alves Biserra, Bruno César Almeida Costa, Samira Esteves Afonso Camargo, Sigmar de Mello Rode, Carolina Judica Ramos, Luciane Dias de Oliveira, Luana Marotta Reis de Vasconcellos

“…The results showed that chronic use of 20% alcohol influenced neither the alkaline phosphatase production nor total protein (p>0.05) in rats, with similar formation of nodules in all groups (p>0.05). …”
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Obesity and fibromyalgia are associated with Difficult-to-Treat Rheumatoid Arthritis (D2T-RA) independent of age and gender by Nicoletta Luciano, Elisa Barone, Enrico Brunetta, Alessio D’Isanto, Maria De Santis, Angela Ceribelli, Marta Caprioli, Giacomo M. Guidelli, Daniela Renna, Carlo Selmi

“…Results Seventy-one/458 (16%) patients fulfilled the 2021 EULAR criteria for D2T-RA with no significant differences for age (median 62 years interquartile range -IQR- 58- 65 vs. 62 IQR 60 – 63 in non-D2T), gender prevalence (23% in both groups) and positivity rates for rheumatoid factors (62% vs. 62% in non-D2T) and Anti-Citrullinated Protein Antibodies (ACPA) (69% vs. 61% in non-D2T). …”
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Schwann Cell‐Specific Ablation of Beclin 1 Impairs Myelination and Leads to Motor and Sensory Neuropathy in Mice by Lisa Gambarotto, Loris Russo, Silvia Bresolin, Luca Persano, Rachele D'Amore, Giulia Ronchi, Federica Zen, Luisa Muratori, Alice Cani, Samuele Negro, Aram Megighian, Sonia Calabrò, Paola Braghetta, Dario Bizzotto, Matilde Cescon

“…Abstract The core component of the class III phosphatidylinositol 3‐kinase complex, Beclin 1, takes part in different protein networks, thus switching its role from inducing autophagy to regulating autophagosomal maturation and endosomal trafficking. …”
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Mechanisms of wogonoside in the treatment of atherosclerosis based on network pharmacology, molecular docking, and experimental validation by Zhaohui Gong, Haixin Yang, Li Gao, Yi Liu, Qingmin Chu, Chuanjin Luo, Liang Kang, Huiqi Zhai, Qiang Xu, Wei Wu, Nan Li, Rong Li

“…This included pathological morphology and lipid deposition analysis of mouse aorta, serum lipid level analysis, Elisa analysis, oxidative stress analysis, reactive oxygen species (ROS) fluorescence assay, immunohistochemical analysis and protein blot analysis. Results Predictions were obtained that wogonoside treatment of atherosclerosis has 31 core targets, which are mainly focused on pathways such as Toll-like receptor (TLR) signaling pathway and NF-kappa B (NF-κB ) signaling pathway. …”
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Significance of KLK7 expression, polymorphisms, and function in sheep horn growth by Mingzhu Shan, Hao Li, Xinyue Li, Guoqing Zhang, Jianxin Shi, Pingjie Feng, Lisheng Zhou, Zhangyuan Pan, Mingxing Chu

“…By comparing the amino acid sequence of KLK7 with KLK7 sequences in other species, we discovered eight amino acids at specific positions in the KLK7 protein sequence that may have regulatory functions in determining the size of horns in ruminant animals. …”
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Associations between skeletal muscle phenotype, positional role, and on‐ice performance in elite male ice hockey players by Jeppe F. Vigh‐Larsen, Hallur Thorsteinsson, Martin Thomassen, Jeppe Panduro, Bjørn Fristrup, Morten B. Randers, Jens L. Olesen, Peter Krustrup, Kristian Overgaard, Lars Nybo, Magni Mohr

“…Resting thigh muscle biopsies were analyzed for metabolic enzyme activity and protein expression linked to performance. On‐ice intermittent exercise capacity, repeated sprint ability, and maximal isometric knee‐extensor torque were also assessed. …”
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Exposure to nano-polystyrene during pregnancy leads to Alzheimer's disease-related pathological changes in adult offspring by Fengxu Wang, Jin Wang, Na Luo, Yonghua Luo, Zhengnan Gao, Ye Cui, Mengna Jiang, Zhaoping Shen, Jing Xiao, Peng Xue, Xiaoke Wang, Shali Yu, Qiyun Wu, Xinyuan Zhao

“…Furthermore, the expression of Aβ protein was markedly increased, consistent with typical AD pathological features. …”
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Waning neutralizing antibodies through 180 days after homologous and heterologous boosters of inactivated COVID-19 vaccine by Zhifei Chen, Fangqin Xie, Hairong Zhang, Dong Li, Suhan Zhang, Mengping Zhang, Junrong Li, Jianfeng Xie, Lina Zhang, Xiuhui Yang, Dongjuan Zhang

“…This cohort study involved 320 healthy individuals, who were randomly assigned to four groups, to receive boosters with inactivated vaccine (COVac and BIBP), the adenovirus type-5-vectored vaccine (Convidecia), and the recombinant protein-based vaccine (Zifivax), respectively, all the vaccines studied had the Wuhan variant as their parental variant. …”
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Molecular mechanisms of aflatoxin neurotoxicity and potential neuroprotective agents by Chongshan Dai, Erjie Tian, Hui Li, Subhajit Das Gupta, Zhihui Hao, Zhanhui Wang, Tony Velkov, Jianzhong Shen

“…Several signaling pathways, including gasdermin D, toll like receptor 2 (TLR2), TLR4, Akt, NF-κB, ERK/MAPK, protein kinase C (PKC), and mitochondrial apoptotic pathways have been shown to participate in AFB1-induced neuronal or astrocyte cell death. …”
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GAMG alleviates liver fibrosis through inducing ferroptosis in inflammatory macrophages via the IRF1/SLC7A11 signaling pathway by Qing Pang, Shuai Zhou, Yong Wang, Hongtao Pan, Zhixin Wang, Xilaing Qin, Chao Zhu, Shilei Chen, Huichun Liu, Xiaosi Hu, Hao Jin

“…In addition, GAMG remarkably decreased the content of collagen protein and improved liver function indicators. Single-cell RNA sequencing revealed that GAMG significantly affected the changes of macrophage subsets in LF, and Funrich analysis identified IRF1 as a key transcription factor regulating the macrophage genome. …”
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Comparison of the Influence of Vegetarian and Mediterranean Diets on the Formation of Kidney Stones by Sebastian Samuła, Michał Mazur, Dorota Waz, Jakub Szarłowicz, Zofia Goliszek, Karolina Łucja Sobek, Wiktoria Tabin-Barczak, Aldona Sokołowska, Klaudia Fikas, Kamil Chwaliszewski

“…It combines the advantages of the vegetarian diet with additional benefits provided by dairy, healthy fats, and moderate animal protein intake. Its comprehensive preventive effects and adaptability to patient needs make it a valuable dietary model for kidney stone prevention and management. …”
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Influence of spouting period on microbiological and nutritional attribute of sesame seed flour by Joseph Oghenewogaga Owheruo, Patrick Othuke Akpoghelie, Great Iruoghene Edo, Emad Yousif, Agatha Ngukuran Jikah, Priscillia Nkem Onyibe, Ufuoma Ugbune, Endurance Fegor Isoje, Ufuoma Augustina Igbuku, Helen Avuokerie Ekokotu, Ephraim Evi Alex Oghroro, Joel Okpoghono, Arthur Efeoghene Athan Essaghah, Joy Johnson Agbo

“…Results showed that increasing the spouting periods of sesame seed significantly (P < 0.05) increased the protein (28.92–44.21 g/100 g) and ash (9.13–9.93 g/100 g) but decreased moisture (8.02–4.67 g/100 g), crude fibre (13.89—9.82 g/100 g), carbohydrate (16.75–4.10 g/100 g) and crude fat (23.29–23.27 g/100 g) of the flour respectively. …”
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Genome-wide identification of MGT gene family in soybean (Glycine max) and their expression analyses under magnesium stress conditions by Ammar Anwar, Junaid Akhtar, Saba Aleem, Maida Aleem, Muhammad Khuram Razzaq, Saud Alamri, Qasim Raza, Iram Sharif, Arooj Iftikhar, Shehreen Naseer, Zaheer Ahmed, Iqrar Ahmed Rana, Waheed Arshad, Muhammad Imran Khan, Javaid Akhter Bhat, Muqadas Aleem, Abdel-Rhman Z. Gaafar, Mohamed S. Hodhod

“…Conclusion This study presents a comprehensive analysis of the MGT gene family in soybean, providing insights into their evolutionary relationships, gene classification, protein structures, and expression patterns under both Mg deficiency and Mg surplus conditions.…”
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Clinicopathological relationship between PM2.5 exposure and acute exacerbations of chronic obstructive pulmonary disease by Anfeng Cui, Yingying Shao, Yang Wang, Haodong Wang, Jiayi Song, Caiji Liu, Ningning Shen, Zhiqing Yang, Wangliang Zhang, Tiane Luo, Chen Wang

“…Inflammatory markers (leukocyte and neutrophil counts, eosinophil percentages, C-reactive protein), D-dimer levels, PaCO2, and pulmonary fibrosis indicators showed positive correlations with PM2.5 levels, whereas Pulmonary Function measures (FEV1/ FVC, FEV1 %) and PaO2 showed inverse correlations. …”
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Small intestinal bacterial overgrowth syndrome and systemic inflammation in pathogenesis of hemodynamic changes at liver cirrhosis by R. V. Maslennikov, A. A. Driga, K. V. Ivashkin, M. S. Zharkova, M. V. Mayevskaya, Ch. S. Pavlov, M. G. Arslanyan, N. B. Musina, Ye. N. Berezina, V. T. Ivashkin

“…Lactulose hydrogen breath test, serum level of C-reactive protein and cardiac ultrasound with simultaneous blood pressure (BP) and heart rate measurement, estimation of basic hemodynamic scores: mean blood pressure (MBP), cardiac output (CO), total peripheral vascular resistance (TPVR) were investigated in all patients. …”
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