Molecular determinants of Neu5Ac binding to a tripartite ATP independent periplasmic (TRAP) transporter by Parveen Goyal, KanagaVijayan Dhanabalan, Mariafrancesca Scalise, Rosmarie Friemann, Cesare Indiveri, Renwick CJ Dobson, Kutti R Vinothkumar, Subramanian Ramaswamy

“…Neu5Ac primarily interacts with residues in the elevator domain of the protein, thereby supporting the elevator with an operator mechanism. …”
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Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation by Yong Yi, Guoqiang Wang, Wenhua Zhang, Shuhan Yu, Junjie Fei, Tingting An, Jianqiao Yi, Fengtian Li, Ting Huang, Jian Yang, Mengmeng Niu, Yang Wang, Chuan Xu, Zhi-Xiong Jim Xiao

“…Mechanistically, glucose deprivation activates Ras signaling to enhance MT-CO2 transcription and inhibits IGF2BP3, an RNA-binding protein, to stabilize MT-CO2 mRNA. Elevated MT-CO2 increases flavin adenosine dinucleotide (FAD) levels in activating lysine-specific demethylase 1 (LSD1) to epigenetically upregulate JUN transcription, consequently promoting glutaminase-1 (GLS1) and glutaminolysis for tumor cell survival. …”
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Effect of HUFA in Enriched Artemia on Growth Performance, Biochemical and Fatty Acid Content, and Hepatopancreatic Features of Penaeus vannamei Postlarvae from a Commercial Shrimp... by Marina Martínez Soler, Gercende Courtois de Vicose, Javier Roo Filgueira, José Zambrano Sánchez, Edwin Yugcha Oñate, Magaly Montachana Chimborazo, Walter Intriago Díaz, Eduardo Reyes Abad, Juan Manuel Afonso López

“…Biochemical composition of postlarvae (lipids, ash, and protein content) did not present differences between the three treatments. …”
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The m6A modification regulates the composition of myofiber types in chicken skeletal muscle by Yanju Shan, Yifan Liu, Ming Zhang, Lichuan Pang, Gaige Ji, Xiaojun Ju, Yunjie Tu, Jingting Shu

“…It is exciting we found that the expression level of ALKBH5 mRNA and protein, was closely related to the composition of myofiber types. …”
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Circulating Galectin-3 Levels Associated with Insulin Resistance in Gestational Diabetes Mellitus: A Cross-sectional Study by Lakshmi Vijukumar, P Renuka, VM Vinodhini, Karthiga J Prabhu, Ashish Kumar, Aryan Kumar Singh

“…Introduction: Gestational Diabetes Mellitus (GDM) is a prevalent pregnancy complication with significant health implications for both the mother and offspring. Galectin-3, a protein expressed in various cells, contributes to cellular and systemic insulin resistance and is secreted into extracellular fluid. …”
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Low versus high initial oral glucocorticoid dose for lupus nephritis: a pooled analysis of randomised controlled clinical trials by Amit Saxena, Peter Izmirly, Jill P Buyon, Cristina Sorrento, Janine Sullivan, Monica Gamez-Perez, Jammie Law, Howard Michael Belmont

“…Complete renal response requiring urine protein-creatinine ratio <0.5 mg/mg (CRR 0.5), CRR or partial renal response (PRR), serious adverse events (SAE) and SAE due to infections at 12 months of treatment were compared between groups.Results 417 patients from SOC arms of five studies were exposed to low-dose initial GC after intravenous pulse, while 521 patients from four studies were treated with high-dose oral GC. …”
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CDK9 Expression Shows Role as a Potential Prognostic Biomarker in Breast Cancer Patients Who Fail to Achieve Pathologic Complete Response after Neoadjuvant Chemotherapy by Ashley J. Schlafstein, Allison E. Withers, Soumon Rudra, Diana Danelia, Jeffrey M. Switchenko, Donna Mister, Saul Harari, Hui Zhang, Waaqo Daddacha, Shahrzad Ehdaivand, Xiaoxian Li, Mylin A. Torres, David S. Yu

“…Immunohistochemistry was performed on patient samples to determine CDK9 expression levels after NACT. Protein expression was linked with clinical data to determine significance. …”
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Selected cannabis cultivars modulate glial activation: in vitro and in vivo studies by Sigal Fleisher-Berkovich, Nitzan Sharon, Yvonne Ventura, Valeria Feinshtein, Jonathan Gorelick, Nirit Bernstein, Shimon Ben-Shabat

“…Materials and methods In vitro experiments were performed with lipopolysaccharide (LPS)-activated BV-2 microglia and primary glial cells to evaluate the effect of different cannabis cultivars on nitric oxide (NO) and inflammatory cytokines, as well as inducible nitric oxide synthase (iNOS) protein expression. An in vivo experiment using the experimental autoimmune encephalomyelitis (EAE) MS model was conducted using Myelin oligodendrocyte glycoprotein (MOG) as the activating peptide. …”
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A relay race of ESCRT-III paralogs drives cell division in a hyperthermophilic archaeon by Junfeng Liu, Mickaël Lelek, Yunfeng Yang, Audrey Salles, Christophe Zimmer, Yulong Shen, Mart Krupovic

“…Depletion of ESCRT-III-2 leads to the accumulation of cells connected through narrow membrane bridges (“late” stage), consistent with the key role of this protein in the final membrane abscission. We used 3D-single molecule localization microscopy to image ESCRT-III rings of different diameters and show that the decrease in the ESCRT-III ring diameter and membrane constriction are inconsistent with a mechanism exclusively based on spiraling of the ESCRT-III filaments. …”
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Expression and Significances of Contactin-1 in Human Gastric Cancer by Ji-Wei Yu, Sheng-Hua Wu, Rui-qi Lu, Ju-gang Wu, Xiao-Chun Ni, Gou-cai Zhou, Hai-guang Jiang, Lin-Hai Zheng, Xiao-Qiang Li, Guang-Ye Du, Bo-Jian Jiang

“…The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot. …”
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Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY12/P2RY13 related functions by Martin Škandík, Lara Friess, Guillermo Vázquez-Cabrera, Lily Keane, Kathleen Grabert, Mireia Cruz De los Santos, Mercedes Posada-Pérez, Austeja Baleviciute, Mathilde Cheray, Bertrand Joseph

“…Microglial purine sensing and motility capacities, regulated by P2RY12/P2RY13, displayed age-related alterations. Remarkably, protein analysis in human brain tissue validated the observed upregulation of MCT4 and downregulation of P2RY12 in aged microglia. …”
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Family Rehabilitation Training by Jinyun Rehabilitation Platform in Patients with Ankylosing Spondylitis Treated by Chinese Medicine Fumigation Combined with Muscle Energy Technolo... by Longbin SHEN, Hui OUYANG, Dongling WU, Wenhao XIE

“…After treatment, the visual analogue score (VAS), rang of motion (ROM), muscle strength, Bath AS disease activity index (BASDAI), Bath AS functional index (BASFI), Schober test score, erythrocyte sedimentation rate (ESR) and C reactive protein (CRP) were compared between the two groups.Results:Before treatment, there was no significant difference in VAS, ROM, muscle strength, BASDAI, BASFI, Schober score, ESR and CRP between the two groups (<italic>P</italic>&gt; 0.05). …”
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Impaired pulmonary function increases the risk of gout: evidence from a large cohort study in the UK Biobank by Zijian Kang, Jianzheng Zhang, Chen Zhu, Ying Zhu, Hanlei Jiang, Qiang Tong, Sheng-Ming Dai

“…Mediation analysis revealed that urate levels (49.1% mediation proportion), C-reactive protein (CRP) levels (6.62%), monocyte counts (1.33%), and neutrophil counts (4.85%) significantly mediated the relationship between pulmonary function and the risk of gout. …”
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Small extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity by Saranyou Oontawee, Parkpoom Siriarchavatana, Watchareewan Rodprasert, Irma Padeta, Yudith Violetta Pamulang, Poorichaya Somparn, Trairak Pisitkun, Boondarika Nambooppha, Nattawooti Sthitmatee, Daneeya Na Nan, Thanaphum Osathanon, Hiroshi Egusa, Chenphop Sawangmake

“…To validate the efficacy of IVES for sEV production, various analyses were conducted, including microscopic examination, surface marker assessment, zeta-potential measurement, protein quantification, nanoparticle tracking analysis, and determination of anti-inflammatory activity. …”
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Adalimumab Treatment in Biologically Naïve Crohn’s Disease: Relationship with Ectopic MUC5AC Expression and Endoscopic Improvement by Tsutomu Mizoshita, Satoshi Tanida, Hironobu Tsukamoto, Keiji Ozeki, Takahito Katano, Hirotaka Nishiwaki, Masahide Ebi, Yoshinori Mori, Eiji Kubota, Hiromi Kataoka, Takeshi Kamiya, Takashi Joh

“…Both mean CD activity index scores and serum C-reactive protein values were significantly lower after ADA therapy (P<0.001). …”
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Effects of Stocking Density on Fatty Acid and Amino Acid Composition in Muscle, Serum Cortisol, Stress and Immune Response in Large Yellow Croaker (<i>Larimichthys crocea</i>) by Youbin Yu, Liang Wang, Wenyun Huang, Duo Yu, Qiaoxuan Sun, Mingcao Cui

“…Levels of cortisol in serum, superoxide dismutase (SOD) and catalase (CAT) in liver, Na⁺-K⁺ ATPase and Na⁺-K⁺ ATPase gene in gills, and heat shock protein (HSP70/90) genes and glutathione S-transferase (GST) genes in the liver significantly increased under HD treatment. …”
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Effect of miR-144-3p-Targeted Regulation of PTEN on Proliferation, Apoptosis, and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells under Stretch by Shiyong Ling, Xi Luo, Bo Lv, Hua Wang, Mengzhi Xie, Kai Huang, Jingchuan Sun

“…The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) colorimetric assay; the expression levels of cyclin, cyclin-dependent kinases (CDK), BCL2-associated X (BAX), B-cell lymphoma-2 (BCL-2), and other cell cycle and apoptosis related proteins were detected by western blot (WB); and the osteogenic differentiation ability of MSC cells was detected by ALP staining and alizarin red staining. …”
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METTL16 is Required for Meiotic Sex Chromosome Inactivation and DSB Formation and Recombination during Male Meiosis by Lisha Yin, Nan Jiang, Wenjing Xiong, Shiyu Yang, Jin Zhang, Mengneng Xiong, Kuan Liu, Yuting Zhang, Xinxin Xiong, Yiqian Gui, Huihui Gao, Tao Li, Yi Li, Xiaoli Wang, Youzhi Zhang, Fengli Wang, Shuiqiao Yuan

“…Mechanistically, in pachytene spermatocytes, METTL16 interacts with MDC1/SCML2 to coordinate DNA damage response (DDR) and XY body epigenetic modifications that establish and maintain MSCI, and in early meiotic prophase I, METTL16 regulates DSB formation and recombination by regulating protein levels of meiosis‐related genes. Furthermore, multi‐omics analyses reveal that METTL16 interacts with translational factors and controls m6A levels in the RNAs of meiosis‐related genes (e.g., Ubr2) to regulate the expression of critical meiotic regulators. …”
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Anti-inflammatory effect of Angiotensin 1-7 in white adipose tissue by Nozomi Nishida, Satoru Sugimoto, Satoshi Miyagaki, Chiharu Cho, Madoka Konishi, Takeshi Goda, Mihoko Yamaguchi, Yasuhiro Kawabe, Hidechika Morimoto, Joji Kusuyama, Takuro Okamura, Masahide Hamaguchi, Jun Mori, Hisakazu Nakajima, Michiaki Fukui, Tomoko Iehara

“…Monocyte chemoattractant protein-1 (MCP-1) produced by white adipocytes and tumour necrosis factor-α (TNF-α) produced by macrophages are pro-inflammatory cytokines and interact to form a pathogenic loop to exacerbate obesity-induced inflammation. …”
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Effects of deoxynivalenol contaminated corn distiller's dried grains with solubles on growth performance, body composition, immunological response, and gastrointestinal health in y... by Deependra Paneru, Milan K. Sharma, Hanyi Shi, Doyun Goo, Venkata S.R. Choppa, Ishwari Gyawali, Revathi Shanmugasundaram, Woo K. Kim

“…Gut health was compromised as gut permeability increased linearly with increasing DDGS inclusion (linear, P = 0.043), aligning with significant alterations in the expression of the tight junction protein occludin (OCLN; P = 0.007). Antioxidant responses in the liver showed increased superoxide dismutase (SOD) activity in early exposure (day 13, P = 0.038), followed by decreased SOD activity (P = 0.001) and reduced glutathione (GSH) levels (P < 0.001) by day 28. …”
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