Uncovering somatic mosaic variants of PIK3CA-related overgrowth disorders – three cases with different clinical presentations by M. Tooming, M. Tooming, P. Mertsina, T. Kahre, T. Kahre, R. Teek, I. Vainumäe, I. Vainumäe, S. Lilles, S. Lilles, M. H. Wojcik, M. H. Wojcik, M. H. Wojcik, P. Ilves, P. Ilves, K. Õunap, K. Õunap

“…IntroductionPIK3CA related disorders (PRD, OMIM: *171834) are genetic disorders resulting from pathogenic somatic mosaic variants in the PIK3CA gene, which encodes a protein crucial for regulating cell growth and division. …”
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Assessments of prostate cancer cell functions highlight differences between a pan‐PI3K/mTOR inhibitor, gedatolisib, and single‐node inhibitors of the PI3K/AKT/mTOR pathway by Adrish Sen, Salmaan Khan, Stefano Rossetti, Aaron Broege, Ian MacNeil, Ann DeLaForest, Jhomary Molden, Laura Davis, Charles Iversrud, Megan Seibel, Ross Kopher, Stephen Schulz, Lance Laing

“…The superior effects of gedatolisib were likely associated with more effective inhibition of critical PAM‐controlled cell functions, including cell cycle, survival, protein synthesis, oxygen consumption rate, and glycolysis. …”
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Serum metabolic profiles in quails: Effects of age, breed, and sex by Qin He, Hanle Shang, Yizhong Huang, Lizhi Li, Zhuomin Wu, Huihui Ran, Jiguo Xu, Xinwei Xiong

“…Quails are important models due to their low-cost protein sources—both eggs and meat—and practical benefits such as minimal space requirements, high egg production, disease resistance, and rapid reproduction. …”
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Geographical Differences in the Safety and Efficacy of Tofacitinib Versus TNFi: A Post Hoc Analysis of ORAL Surveillance by Bogdan Batko, Slawomir Jeka, Piotr Wiland, Agnieszka Zielińska, Maria Stopińska-Polaszewska, Marcin Stajszczyk, Magdalena Kosydar-Piechna, Mary Jane Cadatal, Jose L. Rivas

“…Efficacy endpoints included Clinical Disease Activity Index, Disease Activity Score in 28 joints, with C-reactive protein (DAS28-4[CRP]), and Health Assessment Questionnaire-Disability Index (HAQ-DI). …”
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Frequency and Molecular Characteristics of Mismatch Repair-deficient Status among Multiple Synchronous Colorectal Cancers by Kenichi Chikatani, Noriyasu Chika, Noriko Tanabe, Yoshiko Mori, Okihide Suzuki, Takatoshi Matsuyama, Keiichiro Ishibashi, Hidetaka Eguchi, Yasushi Okazaki, Tatsuro Yamaguchi, Hideyuki Ishida

“…IHC showed a loss of expression of at least one MMR protein in 10 (7.5%) of 133 patients and 17 (5.5%) of 309 lesions. …”
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The plasma metabolome of juvenile idiopathic arthritis varies according to subtype and underlying inflammatory status by Jooa Kwon, Melanie R. Neeland, Justine A. Ellis, Jane Munro, Richard Saffery, Boris Novakovic, Toby Mansell

“…We also assessed the extent to which these signatures are due to underlying inflammation as assessed by glycoprotein acetyls (GlycA) and high-sensitivity C-Reactive Protein (hsCRP) levels. Methods Targeted nuclear magnetic resonance (NMR) metabolomic profiles of plasma of 72 children with JIA and 18 controls were assessed cross-sectionally. …”
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<i>Bacillus thuringiensis</i> Cry1A Insecticidal Toxins and Their Digests Do Not Stimulate Histamine Release from Cultured Rat Mast Cells by Hisashi Ohto, Mayumi Ohno, Miho Suganuma-Katagiri, Takashi Hara, Yoko Egawa, Kazuya Tomimoto, Kosuke Haginoya, Hidetaka Hori, Yuzuri Iwamoto, Tohru Hayakawa

“…Public acceptance of genetically modified crops engineered with <i>Bacillus thuringiensis</i> (Bt) insecticidal protein genes (BT-GMCs), which confer resistance to various lepidopteran insect pests, is generally lacking. …”
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Establishment of a CRISPR-Based Lentiviral Activation Library for Transcription Factor Screening in Porcine Cells by Yingjuan Liang, Xiaoxia Yao, Jingxin Han, Jinpeng Wang, Xiao Zhang, Donglin Zhao, Chaoqian Jiang, Lishuang Geng, Shihao Lv, Zhonghua Liu, Yanshuang Mu

“…Porcine PK15 cells expressing the porcine OCT4 promoter driving EGFP, dCas9 fused with VP64, and MS2-binding protein-p65-HSF1 were constructed, and then, the sgRNA activation library was used to screen the transcription factors regulating OCT4 expression. …”
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Early Postoperative Pain Control and Inflammation for Total Knee Arthroplasty: A Retrospective Comparison of Continuous Adductor Canal Block versus Single-Shot Adductor Canal Block... by Xiaojuan Yang, Jun Dong, Wei Xiong, Fusen Huang

“…Patients in Group B displayed better functional recovery and inflammation results at POH48 than Group A with respect to range of knee flexion and extension (117.8 ± 10.9° vs. 125.2 ± 9.4°, P<0.001) and inflammation cytokines, including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and interleukin-6 (IL-6) ((43.8 ± 16.1) vs. (36.8 ± 13.2), P=0.003; (34.9 ± 9.4 mg/L) vs. (29.6 ± 10.6 mg/L), P=0.001; (21.3 ± 8.7 pg/ml) vs. (14.0 ± 7.0 pg/ml), P<0.001)). …”
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Compost as an eco-friendly alternative to mitigate salt-induced effects on growth, nutritional, physiological and biochemical responses of date palm by Mohamed Ait-El-Mokhtar, Abdessamad Fakhech, Raja Ben-Laouane, Mohamed Anli, Abderrahim Boutasknit, Youssef Ait-Rahou, Said Wahbi, Abdelilah Meddich

“…Furthermore, leaf water status, stomatal conductance and photosynthetic efficiency were increased and were coupled with high chlorophyll and protein concentrations in plants amended with compost under salt stress. …”
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Impact of subgingival periodontal treatment on systemic markers of inflammation in patients with metabolic syndrome: a systematic review of randomized clinical trials by Marie Chavez, Asshly Ramirez, Akram Hernández-Vásquez, Daniel Comandé, Diego Azañedo

“…IntroductionThis study synthesizes evidence on the impact of subgingival periodontal treatment combined with antibiotics on reducing systemic inflammation markers—C-reactive protein (CRP), interleukins, and tumor necrosis factor-alpha (TNF-α)—in patients with metabolic syndrome (MS) and periodontal disease (PD), compared to supragingival periodontal treatment with placebo.MethodsRandomized clinical trials (RCTs) published in English, Spanish, or Portuguese that addressed the research question were included. …”
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Nutritional Properties, Antioxidant and Antihaemolytic Activities of the Dry Fruiting Bodies of Wild Edible Mushrooms Consumed by Ethnic Communities of Northeast India by Merilin Kakoti, Dibya Jyoti Hazarika, Assma Parveen, Samim Dullah, Alokesh Ghosh, Dipankar Saha, Madhumita Barooah, Robin Chandra Boro

“…., respectively), crude protein content (20.72 g/100 g d.w. and 20.54 g/100 g d.w., respectively) and a considerable content of minerals. …”
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Total flavonoids of Astragalus membranaceus protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity in mice by inhibiting ferroptosis through SLC7A11/GPX... by Zitian Gao, Gaorui Wang, Yujie Chen, Wuke Yuan, Jun Cai, Aiping Feng, Jie Fang, Qi Xu, Xiaojun Wu

“…TFA offered neuroprotection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in the mouse model of Parkinsonism, by improving behavior performance in the gait analysis and pole test, and inhibiting the decline of tyrosine hydroxylase (TH) positive neurons and TH protein expression in substantia nigra of mice. TFA also prevented 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in SH-SY5Y cells, by increasing GSH and GSH/GSSG ratio, and reducing reactive oxygen species. …”
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The impact of dietary phosphorus levels on growth, slaughter, and digestive metabolism in growing sheep by Shoupei Zhao, Xiaojun Ni, Jia Zhou, Xiaoqi Zhao, Xiao Wen, Xiaolin Wang, Mingyu Cao, Yanfei Zhao, Chong Shao, Lianghao Lu, Yuanyuan Chen, Bao Zhang, Huaming Yang, Bai Xue, Guobo Quan

“…P levels also affected P, calcium, protein, and energy metabolism, as well as apparent digestibility of acid detergent fiber (p &lt; 0.05). …”
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Fasting activates optineurin-mediated mitophagy in chondrocytes to protect against osteoarthritis by Min-Na Zhang, Ran Duan, Gui-Hong Chen, Mei-Jun Chen, Chun-Gu Hong, Xin Wang, Zhi-Lin Pang, Chun-Yuan Chen, Hua-Feng Liu, Da Zhong, Hui Xie, Wen-Bao Hu, Zheng-Zhao Liu

“…Here, we aimed to investigate the role of the autophagy receptor protein optineurin (OPTN) in OA, and explore the effects of dietary intervention on OA symptoms and its relationship with OPTN-mediated mitophagy. …”
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Lipid-encapsulated gold nanoparticles: an advanced strategy for attenuating the inflammatory response in SARS-CoV-2 infection by Sanjeevram Dhandapani, Yujeong Ha, Rongbo Wang, Tae Woo Kwon, Ik-Hyun Cho, Yeon-Ju Kim

“…KGS-NE oral administration facilitated the recovery of mice induced with the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, as confirmed by inflammatory markers expression in lung tissue. …”
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Effects of subclinical mastitis on automatic milking system data, hematological and biochemical parameters, and milk composition in Holstein cows by Mooyoung Jung, Seogjin Kang, Eunjeong Jeon, Dong-Hyun Lim, Donghyeon Kim, Jin San Moon, Sang-Hwan Hyun, Seungmin Ha

“…The mCON group had higher levels of milk non-protein nitrogen than the mNQ group (p<0.05). Opportunistic mastitis pathogens were isolated in the mIQ group. …”
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Butyrylcholinesterase Levels on Admission Predict Severity and 12-Month Mortality in Hospitalized AIDS Patients by Lijun Xu, Biao Zhu, Ying Huang, Zongxing Yang, Jia Sun, Yan Xu, Jinlei Zheng, Sabine Kinloch, Michael T. Yin, Honglei Weng, Nanping Wu

“…Associations between BChE level and CD4 count, WHO stage, body mass index (BMI), C-reactive protein (CRP) level, and duration of hospitalization were assessed. …”
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Associations of the levels of adipokines and cytokines in individual follicles with in vitro fertilization outcomes in women with different ovarian reserves by Xuelan Li, Chujun Li, Jie Yang, Min Lin, Xianli Zhou, Ziyang Su, Yuting Zhang, Xinning Li, Xin Chen

“…The levels of 5 adipokines (including visfatin-1, monocyte chemoattractant protein-1 [MCP-1], resistin, leptin, and chemerin) and 3 cytokines (including interleukin [IL]-6, IL-12p70, and tumor necrosis factor [TNF]-α) in FF were determined by Luminex technology. …”
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ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1 by Živa Frangež, Deborah Gérard, Zhaoyue He, Marios Gavriil, Yuniel Fernández-Marrero, Yuniel Fernández-Marrero, S. Morteza Seyed Jafari, Robert E. Hunger, Philippe Lucarelli, Shida Yousefi, Thomas Sauter, Lasse Sinkkonen, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon

“…Autophagy is a highly conserved cellular process in which intracellular proteins and organelles are sequestered and degraded after the fusion of double-membrane vesicles known as autophagosomes with lysosomes. …”
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