Icariin modulates osteogenic and adipogenic differentiation in ADSCs via the Hippo-YAP/TAZ pathway: a novel therapeutic strategy for osteoporosis by Shaozi Lin, Zuyu Meng, Mei Wang, Zixuan Ye, Mengsha Long, Yiyao Zhang, Fang Liu, Hongling Chen, Menghan Li, Jiajia Qin, Haiquan Liu

“…Additionally, ADSCs were labeled with lentiviruses carrying enhanced green fluorescent protein (EGFP) and 5-bromo-2-deoxyuridine (BrdU) to assess their in vivo distribution, survival, proliferation, and differentiation of ADSCs post-ICA intervention.ResultsIn vitro, ICA significantly inhibited the Hippo pathway, reducing YAP and TAZ phosphorylation and enhancing their transcriptional activity, while simultaneously suppressing PPARγ. …”
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Identification of SETD4 as an Onco‐Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers by Yuyun Zhong, Ruiqi Wang, Zijie Huang, Zhaoting Hu, Bin Peng, Bin Chen, Liyue Sun

“…ABSTRACT Background SET domain‐containing protein 4 (SETD4) is a histone methyltransferase that has been shown to modulate cell proliferation, differentiation, and inflammatory responses by regulating histone H4 trimethylation (H4K20me3). …”
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Dorsal Root Ganglion Maintains Stemness of Bone Marrow Mesenchymal Stem Cells by Enhancing Autophagy through the AMPK/mTOR Pathway in a Coculture System by Shuaishuai Zhang, Junqin Li, Huijie Jiang, Yi Gao, Pengzhen Cheng, Tianqing Cao, Donglin Li, Jimeng Wang, Yue Song, Bin Liu, Hao Wu, Chunmei Wang, Liu Yang, Guoxian Pei

“…In addition, AMPK inhibitor compound C could significantly downregulate the protein expression of LC3 and the mRNA level of stemness genes in the coculture group. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…Similarly, HIOs elicited strong mRNA and protein mesenchymal (CXCL10) and epithelial (CXCL1, CCL2, CXCL8, and CCL20) chemokine responses in early but not late passages. …”
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The serum level of sclerostin decreases in radiographic axial spondyloarthritis patients with fatty lesions by Xuegang Li, Haijian Jiang, Xu Wang, Shuping Zhong

“…Results There were no significant differences between two groups in terms of gender, age, body mass index (BMI), duration, smoking, HLA-B27 positivity rate, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), BASDAI, ASDAS-ESR, ASDAS-CRP, biological disease-modifying anti-rheumatic drugs (bDMARDs) rate. …”
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Microsatellite instability and somatic gene variant profile in solid organ tumors by Ibrahim Halil Erdogdu, Seda Orenay-Boyacioglu, Olcay Boyacioglu, Nesibe Kahraman-Cetin, Habibe Guler, Merve Turan, Ibrahim Meteoglu

“…This study aimed to determine the MSI status, MMR protein expression, and somatic mutation profile in solid organ tumors. …”
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Evaluation of Tissue Tropism and Horizontal Transmission of a Duck Enteritis Virus Vectored Vaccine in One-Day-Old Chicken by Yassin Abdulrahim, Yingying You, Linggou Wang, Zhixiang Bi, Lihua Xie, Saisai Chen, Benedikt B. Kaufer, Armando Mario Damiani, Kehe Huang, Jichun Wang

“…Moreover, DEV-H5 replication in intestinal cells caused an increment in interferon-α expression, while occluding junction proteins and ZO-1 expression were significantly upregulated. …”
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Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae by Théophile Niault, Ariel Talavera, Eric Le Cam, Sonia Baconnais, Ole Skovgaard, Florian Fournes, Léa Wagner, Hedvig Tamman, Andrew Thompson, Dannele Echemendia-Blanco, Noa Guzzi, Abel Garcia-Pino, Didier Mazel, Marie-Eve Val

“…Abstract The replication of the two chromosomes in the pathogenic bacterium Vibrio cholerae is coordinated by the binding of initiator protein RctB to a checkpoint sequence, crtS. Replication of crtS on the primary chromosome (Chr1) triggers replication of the secondary chromosome (Chr2), but the details are poorly understood. …”
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An intein-split transactivator for intersectional neural imaging and optogenetic manipulation by Hao-Shan Chen, Xiao-Long Zhang, Rong-Rong Yang, Guang-Ling Wang, Xin-Yue Zhu, Yuan-Fang Xu, Dan-Yang Wang, Na Zhang, Shou Qiu, Li-Jie Zhan, Zhi-Ming Shen, Xiao-Hong Xu, Gang Long, Chun Xu

“…Here, we utilized the protein-splicing technique mediated by intervening sequences (intein) and devised an intein-based intersectional synthesis of transactivator (IBIST) to selectively control gene expression of common effectors in multiple-feature defined cell types in mice. …”
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Demethylnobiletin and its major metabolites: Efficient preparation and mechanism of their anti-proliferation activity in HepG2 cells by Yanping Xin, Ting Zheng, Man Zhang, Ruiqiang Zhang, Siyue Zhu, Dongli Li, Denggao Zhao, Yanyan Ma, Chi-Tang Ho, Qingrong Huang

“…The expression of cleaved caspase-3 was the highest in cells treated with 5,4'-DDMN, implying that the apoptosis induced by other OH-PMFs might be mediated by other apoptotic execution proteins. Our research reveals the application potential and scientific evidence for the production and functionality of OH-PMFs.…”
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Genome-Wide Identification and In Silico Expression Analysis of <i>CCO</i> Gene Family in <i>Citrus clementina</i> (Citrus) in Response to Abiotic Stress by Sadaf Sarwar, Adnan Sami, Muhammad Zeshan Haider, Layba Tasawar, Jannat Akram, Arsalan Ahmad, Muhammad Shafiq, Haitham E. M. Zaki, Gabrijel Ondrasek, Muhammad Shafiq Shahid

“…To identify their structural features, they employed phylogenetic analysis, protein interactions, localization, structure, miRNA targets, evolutionary analysis, and transcriptome studies. …”
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Biorefining Brazilian Green Propolis: An Eco-Friendly Approach Based on a Sequential High-Pressure Extraction for Recovering High-Added-Value Compounds by Guilherme Dallarmi Sorita, Wilson Daniel Caicedo Chacon, Monique Martins Strieder, Camilo Rodriguez-García, Alcilene Monteiro Fritz, Silvani Verruck, Germán Ayala Valencia, José A. Mendiola

“…SFE showed higher selectivity, resulting in a lower carbohydrate content in the non-polar fraction, and PLE extracted nearly twice the protein amount of LPE–2. Despite the HPE selectivity, LPE extracts exhibited better acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and LOX inhibition, demonstrating that the neuroprotective and anti-inflammatory activity of the extracts may be associated with a symbiosis of a set of compounds. …”
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Molecular Characterization and Risk Factors of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae Isolated from Chinese Tertiary Hospital by Zhu R, Li J, Lian S, Li Y, Cai M, Cao Y, Xu X

“…A PCR assay was used to detect the K capsule type, virulence genes, carbapenemase genes, and membrane pore protein. ERIC-PCR was carried out for homology analysis. …”
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Comparison of growth hormone therapy response according to the presence of growth hormone deficiency in children born small for gestational age with short stature in Korea: a retro... by Ha Young Jo, Hyun Ji Jang, Chong Kun Cheon, Ju Young Yoon, Sukdong Yoo, Jung Hyun Lee, Jeong Eun Lee, Ye Jin Kim, Sejin Kim, Hyun-Ji Kim, Im Jeong Choi, Min Jung Kwak

“…The SDS for insulin-like growth factor-1 (IGF-1) was lower among boys without GHD than among boys with GHD, while the SDS for insulin-like growth factor-binding protein-3 (IGFBP-3) was higher among girls without GHD than among girls with GHD; however, there was no significant difference when comparing all children with GHD to those without GHD. …”
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Dietary Nigella sativa nanoparticles enhance broiler growth performance, antioxidant capacity, immunity, gene expression modulation, and cecal microbiota during high ambient temper... by Ahmed M. Elbaz, Eman S. Ashmawy, M. A. A. Farahat, Ahmed Abdel‑Maksoud, Shimaa A. Amin, Zangabel S. Mohamed

“…Despite that feed intake was not affected, feed conversion ratio, body weight gain, and crude protein digestibility improved in broilers fed Nano-NS (P < 0.05) compared with avilamycin and the control groups. …”
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Computational insights into Sitahe (Leuconotis eugenifolia) bioactive compounds: A promising approach for radioprotection through p53 inhibition by Dian Pribadi Perkasa, Iin Kurnia, Taufik Muhammad Fakih, Teja Kisnanto, Dwi Ramadhani, Harry Nugroho Eko Surniyantoro, Boky Jeanne Tuasikal, Alfian Mahardika Forentin, Mukh Syaifudin

“…The results demonstrated that the selected compounds maintained stable molecular dynamics profiles, showing consistent interactions with the target protein throughout the simulation period. Among the compounds tested, baurenol exhibited the best performance with a MM/PBSA binding energy of −68.568 kJ/mol, which is better than rhazinilam (−63.538 kJ/mol). …”
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Insights into the Mode of Action of Novel Morpholinated Curcumin Derivatives Exhibiting Potent Antitumor Activity in Bladder Cancer Cells In Vitro by Paulina Kobylka, Pawel Bakun, Joanna Kuzminska, Tomasz Goslinski, Marek Murias, Malgorzata Kucinska

“…The anticancer activity of <b>2a</b> and <b>2a-B</b> was determined using MTT (hypoxic conditions) and LDH (normoxic conditions) assays. An ELISA-based protein panel was used to find the potential molecular targets, while flow cytometric, colorimetric, fluorescent, and luminescent assays were used to investigate the cell death mechanism. …”
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Association of biochemical indicators with multimorbidity in 19,624 older adult individuals with chronic diseases: a study from Jindong District, Jinhua City, China by Qihuan Yao, Guozhong Chen

“…Among the biochemical indicators, hemoglobin (Hb) (OR = 1.46, 95%CI: 1.13–1.90), white blood cell count (WBC) (OR = 1.25, 95%CI: 1.02–1.54), red blood cell count (RBC) (OR = 1.36, 95%CI: 1.10–1.69), urinary protein (U-PRO) (OR = 1.10, 95%CI: 1.02–1.19), urinary glucose (U-GLU) (OR = 1.44, 95%CI: 1.23–1.67), alanine aminotransferase (ALT) (OR = 1.71, 95%CI: 1.39–2.10), aspartate aminotransferase (AST) (OR = 1.22, 95%CI: 1.05–1.41), creatinine (Cr) (OR = 1.28, 95%CI: 1.16–1.42), uric acid (UA) (OR = 1.36, 95%CI: 1.22–1.51), total cholesterol (TC) (OR = 1.76, 95%CI: 1.59–1.95), triglycerides (TG) (OR = 2.63, 95%CI: 2.46–2.82), low-density lipoprotein cholesterol (LDL-C) (OR = 1.84, 95%CI: 1.60–2.11), high-density lipoprotein cholesterol (HDL-C) (OR = 10.99, 95%CI: 8.12–14.90), and fasting blood glucose (FBG) (OR = 1.89, 95%CI: 1.74–2.05) are associated with the risk of multimorbidity of chronic diseases (p &lt; 0.05). …”
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Synergistic fermentation of vitamin B2 (riboflavin) bio-enriched soy milk: optimization and techno-functional characterization of next generation functional vegan foods by Ananya Rana, Neetu Kumra Taneja, Anupama Singh, Tejpal Dhewa, Vikram Kumar, Ankur Kumar, Komal Chauhan, Vijay Juneja, Harinder Singh Oberoi

“…Additionally, techno-functional characterization, including rheology and texture profile analysis, showed that enhanced protease activity of co-cultured LAB improved protein hydrolysis (6259 nm), positively impacting the water holding capacity (WHC) and overall acceptability of the fermented soymilk. …”
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The Treatment Effect of Plantago Major on Lung Cancer Based on the Computed Tomography and Pathological Findings: A Case Report Study by Saade Abdalkareem Jasim, Bashar Mudhaffar Abdullah, Patricio Yánez-Moretta, Wesam R. Kadhum, Yasser Fakri Mustafa, Enas R. Alwaily, Munther Abosoda, Ali Alsalamy, Sada Ghalib Al-Musawi, Alaa A. Omran

“…Materials and Methods: A 64-year-old woman, with gastrointestinal bleeding as well as high Erythrocyte Sedimentation Rate (ESR) and C-Reactive Protein (CRP) levels, was examined by the chest CT scan for suspicious lung cancer. …”
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