Age-related immune response disparities between adults and children with severe COVID-19: a case–control study in China by Hongliang Chen, Hongliang Chen, Yuan Li, Liping Yuan, Fen Liu, Qian Sun, Qian Sun, Qingkai Luo, Yefei Lei, Yinglan Hou, Jiayan Li, Liang Cai, Shixing Tang

“…A case–control study was conducted using propensity score matching (PSM) to mitigate possible confounding factors.ResultsThe significant differences observed included lymphocyte exhaustion, an increased neutrophil-to-lymphocyte (NEU/LYM) ratio, high levels of C-reactive protein (CRP), and a cytokine storm, characterized by high levels of Th1 proinflammatory cytokines, including interleukin 1β (IL-1β), IL-6, IL-8, interferon type I (IFN-γ), and tumor necrosis factor (TNF-α) in the lung among severe adult COVID-19 patients. …”
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Relation of Wnt Signaling Pathway Inhibitors (Sclerostin and Dickkopf-1) to Left Ventricular Mass Index in Maintenance Hemodialysis Patients by Ahmed Bahie, Mohamed M. Abdalbary, Dalia Younis El-Sayed, Rasha Elzehery, Ghada El-Said, Ghada El-Kannishy, Ahmed M. Abd El Wahab

“…Several inhibitors regulate Wnt canonical pathways like Dickkopf-related protein-1 (Dkk-1) and sclerostin. Objectives. To investigate the relationship between serum sclerostin, Dkk-1, left ventricular mass (LVM), and LVM index (LVMI) in HD patients. …”
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Hyperglycemia Does Not Affect Iron Mediated Toxicity of Cultured Endothelial and Renal Tubular Epithelial Cells: Influence of L-Carnosine by Shiqi Zhang, Emmanouil Ntasis, Sarah Kabtni, Jaap van den Born, Gerjan Navis, Stephan J. L. Bakker, Bernhard K. Krämer, Benito A. Yard, Sibylle J. Hauske

“…HG did not change the expression of divalent metal transporter 1 (DMT1), ferroportin (IREG), and transferrin receptor protein 1 (TFRC). Irrespective of glucose concentrations L-carnosine prevented toxicity in a dose-dependent manner, only if it was present during the FeCl3 challenge. …”
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Glucometabolic-Related Genes as Diagnostic Biomarkers and Therapeutic Targets for Alzheimer’s Disease and Type 2 Diabetes Mellitus: A Bioinformatics Analysis by Shuo Liu, He Chen, Xiao-Dong He, Xiao-Ou Yang

“…A range of bioinformatics tools were employed for functional annotation, pathway enrichment, protein interaction network construction, module analysis, ROC curve assessment, correlation matrix construction, gene set enrichment analysis, and gene-drug interaction mapping of these DEGs. …”
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Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi007-A) from a patient with Kennedy disease by Bo Li, Yitong Yang, Yingxin Wang, Didi Shan, Jianing Li, Hongxu Wang, Xiaohan Sun, Yao Tang, Yichang Jiao, Xinbo Ji, Zexin Zhan, Bo Kong, Bo Gao, Yu Wang, Ping Sun, Fuchen Liu

“…The pluripotency of these iPSCs was verified by the expression of several pluripotency markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.…”
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Improved Predictability of Diagnosis and Prognosis Using Serum‐ and Tissue‐Derived Extracellular Vesicles From Bulk mRNA Sequencing in Pancreatic Ductal Adenocarcinoma by Qian Zhu, Zhang Chen, Ming Tian, Xin Yan, Xiangdong Gongye, Zhicheng Liu, Anbang Zhao, Zhiyong Yang, Yufeng Yuan

“…Materials & Methods Exosomes were isolated from healthy individuals and PDAC patients via tissue and serum samples, then identified by analyzing their particle size and protein content. PDAC‐specific exosomal miRNAs were identified using a microRNA array. …”
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Development of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting Orientia tsutsugamushi by Qingyu Lu, Shiyin Yu, Sibo Wang, Min Cao, Liuxin Li, Miao Xin, Weilong Tan, Yong Qi, Yichen Lu, Xiaohui Xiong

“…Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of O. tsutsugamushi were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. …”
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Epithelial cell diversity and immune remodeling in bladder cancer progression: insights from single-cell transcriptomics by Jianpeng Li, Yunzhong Jiang, Minghai Ma, Lu Wang, Minxuan Jing, Zezhong Yang, Mengzhao Zhang, Ke Chen, Jinhai Fan

“…Bulk RNA-seq data were used to validate the clinical features of characteristic cells, and protein levels of these cells were further confirmed through immunohistochemistry (IHC) and multiplex immunofluorescence. …”
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Advancements in functional tetrahedral DNA nanostructures for multi-biomarker biosensing: Applications in disease diagnosis, food safety, and environmental monitoring by Yun Qiu, Yixing Qiu, Wenchao Zhou, Dai Lu, Huizhen Wang, Bin Li, Bin Liu, Wei Wang

“…This review highlights recent advancements in functional tetrahedral DNA nanostructures (FTDN) for various biomarkers monitoring, including nucleic acid, protein, mycotoxin, agent, and metal ion. Additionally, it discusses the potential of FTDN in the fields of disease diagnosis, food safety, and environmental monitoring. …”
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Verification of the Reliability of an Automated Urine Test Strip Colorimetric Program Using Colorimetric Analysis: Survey Study by Keigo Inagaki, Daisuke Tsuriya, Takuya Hashimoto, Katsumasa Nakamura

“…ResultsCompared with the judgments of an automatic urine analyzer, the average agreement rate for 12 items (protein, glucose, urobilinogen, bilirubin, ketone bodies, specific gravity, occult blood, pH, white blood cells, nitrite, creatinine, and albumin) was 78.6%. …”
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Ketogenic diet in psychiatry: application in treating depression among athletes by Paulina Kumięga, Zuzanna Kudas, Nikola Perchel, Aleksandra Litwin, Karolina Krzywicka, Paweł Nowocin, Piotr Wasiński, Natalia Dąbrowska, Martyna Koszyk, Dawid Kulczyński

“…Emerging evidence suggests that the ketogenic diet (KD), a high-fat, moderate-protein, and low-carbohydrate dietary intervention, holds therapeutic potential for managing depression. …”
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Genome-wide microsatellite characterization and their marker development and transferability in Broussonetia Species by Xiaowen Jia, Hanyu Li, Ying Han, Lu Wang, Chanjuan Lai, Xi Liu, Pan Li, Zupei Lei, Yonghua Zhang

“…Genic-mapped SSRs may regulate biological processes by influencing gene activity and protein function. To verify SSRs polymorphism, we selected 30 ones from 10,752 potentially polymorphic SSRs loci for PCR amplification among these three species, all of which were successfully amplified and exhibited polymorphism across these three species. …”
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Deletion of ESX-3 and ESX-4 secretion systems in Mycobacterium abscessus results in highly impaired pathogenicity by Wassim Daher, Vincent Le Moigne, Yara Tasrini, Shweta Parmar, Danielle L. Sexton, John Jairo Aguilera-Correa, Valentin Berdal, Elitza I. Tocheva, Jean-Louis Herrmann, Laurent Kremer

“…Abstract Type VII secretion systems participate in protein export, virulence, conjugation, and metabolic regulation. …”
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Target-specific peptides for BK virus agnoprotein identified through phage display screening: advancing antiviral therapeutics by Xiaofei Xing, Jingxian Han, Keke Wang, Fuyun Tian, CuiXia Jiang, Wei Liang, Lin Qi, Xin Yue, Yinhang Wen, Yuwei Hu, Hui Qiao

“…Agnoprotein, a small, positively charged protein encoded by the BK virus late gene, functions in the assembly, maturation, and release of the virus. …”
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Identification of a binding site for small molecule inhibitors targeting human TRPM4 by Babatunde Ekundayo, Prakash Arullampalam, Christian E. Gerber, Anne-Flore Hämmerli, Sabrina Guichard, Mey Boukenna, Daniela Ross-Kaschitza, Martin Lochner, Jean-Sebastien Rougier, Henning Stahlberg, Hugues Abriel, Dongchun Ni

“…Abstract Transient receptor potential (TRP) melastatin 4 (TRPM4) protein is a calcium-activated monovalent cation channel associated with various genetic and cardiovascular disorders. …”
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Enhancement of Cytomegalovirus-Specific Cytokine Production after Modulation of the Costimulation in Kidney Transplant Patients by Theresa Dornieden, Benjamin Wilde, Johannes Korth, Kai Werner, Peter A. Horn, Oliver Witzke, Monika Lindemann

“…To address this question, lymphocytes of kidney transplant patients were stimulated with CMV-specific antigens and incubated with programmed death-ligand 1 (PD-L1), programmed cell death protein 1 (PD-1), or B- and T-lymphocyte attenuator (BTLA) antibodies. …”
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Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells by Junying Lu, Keyong Fang, Shiji Wang, Lingxin Xiong, Chao Zhang, Zhongmin Liu, Xuewa Guan, Ruipeng Zheng, Guoqiang Wang, Jingtong Zheng, Fang Wang

“…To further confirm the targeted pathway of CBT, we pretreated PBMC with the selective NOD1 inhibitor ML130 and then measured the protein levels of the pathway by Western blotting. …”
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Protective effects of seed oil from Nicotiana tabacum L. On D-galactose induced liver and brain damages of mice by regulating PI3K/Akt/Nrf2 signaling pathway by Ji Gu, Yongchao Wang, Shanshan Xiao, Yunye Zheng, Zhengxuan Wang, Yaping Liu, Limeng Zhang, Guiguang Cheng

“…Western blotting results showed that NC89 and BS4 increased the ratios of p-PI3K/PI3K and p-Akt/Akt, as well as the protein expression of Nrf2, NQO1 and HO-1 in brain tissues. …”
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655Val and 1170Pro ERBB2 SNPs in Familial Breast Cancer Risk and BRCA1 Alterations by Stefania Tommasi, Vita Fedele, Rosanna Lacalamita, Michele Bruno, Francesco Schittulli, David Ginzinger, Gery Scott, Serenella Eppenberger-Castori, Daniele Calistri, Silvia Casadei, Ian Seymour, Salvatore Longo, Gianluigi Giannelli, Brunella Pilato, Giovanni Simone, Christopher C. Benz, Angelo Paradiso

“…Another SNP, Ala1170Pro, introduces a structural change in the carboxyl-terminal regulatory domain of the protein, but its clinical and biological importance remains undefined. …”
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Elevated Cerebrospinal Fluid Neopterin Concentration Is Associated with Disease Severity in Acute Puumala Hantavirus Infection by Timo Hautala, Terhi Partanen, Tarja Sironen, Saara-Mari Rajaniemi, Nina Hautala, Olli Vainio, Olli Vapalahti, Heikki Kauma, Antti Vaheri

“…The neopterin levels were elevated (>5.8 nmol/L) in 22 (96%) NE-patients (mean 45.8 nmol/L); these were especially high in patients with intrathecal PUUV-IgM production (mean 58.2 nmol/L, P=0.01) and those with elevated CSF protein concentrations (mean 63.6 nmol/L, P<0.05). …”
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