Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone by Aras Syazili, . Irmawati, . Alimuddin, Komar Sumantadinata

“…Keywords: recombinant protein, growth hormone, immersion frequency, giant gourami…”
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Human Platelets Take up Anti-VEGF Agents by B. Sobolewska, B. Fehrenbacher, P. Münzer, H. Kalbacher, S. Geue, Konstantinos Stellos, M. Schaller, F. Ziemssen

“…The role of actin filaments and clathrin-coated vesicles in the transport of ranibizumab, aflibercept, and bevacizumab was evaluated by two pharmacologic inhibitors: staurosporine (protein kinase C inhibitor) and cytochalasin D. Results. …”
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Genetic Variations of Gene Associated with Adverse Drug Reactions to Valproic Acid in Korean Epileptic Patients by Ji Hyun Yi, Yang-Je Cho, Won-Joo Kim, Min Goo Lee, Ji Hyun Lee

“…The multidrug resistance protein 2 (MRP2, ABCC2) gene may determine individual susceptibility to adverse drug reactions (ADRs) in the central nervous system (CNS) by limiting brain access of antiepileptic drugs, especially valproic acid (VPA). …”
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Circ-ADAM9 Promotes High Glucose-Induced Retinal Pigment Epithelial Cell Injury in DR via Regulating miR-338-3p/CARM1 Axis by Zeyu Liang, Chengzhe Lu, Tingting Feng, Xiang Gao, Yongfang Tu, Wenchao Yang, Yipeng Wang

“…Cell proliferation and apoptosis were determined using MTT assay, EdU assay, and flow cytometry. The protein expression of apoptosis markers and CARM1 was examined by the western blot analysis. …”
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Valorization of Oil Cakes in Two-Pot Lactone Biosynthesis Process by Jolanta Małajowicz, Agata Fabiszewska, Bartłomiej Zieniuk, Joanna Bryś, Mariola Kozłowska, Katarzyna Marciniak-Lukasiak

“…Their chemical composition (including high fat or protein content, a favorable fatty acid profile, and a high proportion of unsaturated acids) makes them valuable raw materials not only in animal feeding but are increasingly gaining popularity in biotechnological processes. …”
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Genetic and biochemical characterization of a radical SAM enzyme required for post-translational glutamine methylation of methyl-coenzyme M reductase by Roy J. Rodriguez Carrero, Cody T. Lloyd, Janhavi Borkar, Shounak Nath, Liviu M. Mirica, Satish Nair, Squire J. Booker, William Metcalf

“…Structural characterization of the Ni(II) form of Gln-methylated M. acetivorans MCR revealed no significant differences in the protein fold between the modified and unmodified enzyme; however, the purified enzyme contained the heterodisulfide reaction product, as opposed to the free cofactors found in eight prior M. acetivorans MCR structures, suggesting that substrate/product binding is altered in the modified enzyme. …”
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Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus by María del Carmen Monroy-Dosta, Daniel Becerril-Cortés, Juan Pablo Lazo, Arturo Mena-López, Pilar Negrete-Redondo, Eliasid Nogueda-Torres, Carmen Navarro-Guillén, José Antonio Mata-Sotres

“…During 60 days, fish were feed three times a day (zeitgeber time, ZT: 0, 4, and 8) with a fishmeal-based diet containing 32% of crude protein and 5% of lipid, where molasses was added as a carbon source in BFT. …”
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A study looking at how missed somatropin injections affect growth in children with growth hormone deficiency: a plain language summary of publication by Jane Loftus, Bradley S. Miller, Craig S. Parzynski, Jose Alvir, Yong Chen, Priti Jhingran, Anu Gupta, Mitch DeKoven, Victoria Divino, Jenny Tse, Jing He, Michael Wajnrajch

“…Glossary of commonly used terms Adherence – in this study, adherence refers to whether the children had enough medication ( somatropin ) to have daily injections as prescribed by their doctor. Growth hormone - a protein made in the pituitary gland that helps the body grow properly. …”
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Circulating Sclerostin and Irisin Are Related and Interact with Gender to Influence Adiposity in Adults with Prediabetes by Theerawut Klangjareonchai, Hataikarn Nimitphong, Sunee Saetung, Nuttapimon Bhirommuang, Rattanapan Samittarucksa, Suwannee Chanprasertyothin, Rattana Sudatip, Boonsong Ongphiphadhanakul

“…Sclerostin, an osteocyte-specific protein, has been found to be related to adiposity and glucose metabolism. …”
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Pulmonary Innate Immune Response and Melatonin Receptors in the Perinatal Stress by Janaínna Grazielle Pacheco Olegário, Marcos Vinícius Silva, Juliana Reis Machado, Laura Penna Rocha, Marlene Antônia Reis, Camila Souza de Oliveira Guimarães, Rosana Rosa Miranda Corrêa

“…The percentages of IL-6, C-reactive protein (CRP), IL-1β, TNF-α, and melatonin receptor were evaluated by immunohistochemistry. …”
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Relationship between klotho, neurotrophic factors (BDNF, NGF, GDNF) and cognitive functions in patients with bipolar disorder by Zeynep Çelebi, Esra Yazıcı, Derya Güzel Erdoğan, Onur Davutoglu, Ahmet Bulent Yazıcı

“…The Stroop test and Wechsler Memory Scale–Visual Production Subtest were applied, and the serum levels of Klotho, BDNF, GDNF, and NGF were measured with an ELISA reader. Results The klotho protein levels (0.12 ± 0.15 and 0.17 ± 0.16) and NGF levels (34.36 ± 41.99 and 48.54 ± 41.06) in the patient group were significantly lower than those in the control group (Z = -3.071, p = 0.002 and Z = -2.217, p = 0.027, respectively). …”
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miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells by Roberta Sanguineti, Alessandra Puddu, Massimo Nicolò, Carlo Enrico Traverso, Renzo Cordera, Giorgio L. Viviani, Davide Maggi

“…Then, we analyzed mRNA levels of miR-126, VEGF-A, PI3KR2, and SPRED1. We also evaluated protein amount of HIF-1α, PI3KR2, and SPRED1 and VEGF-A secretion. …”
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Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat by B. S. Muhlhausler, R. Cook-Johnson, M. James, D. Miljkovic, E. Duthoit, R. Gibson

“…Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid-Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor-γ (PPARγ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. …”
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AICAR Protects against High Palmitate/High Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression by Ricardo Rodríguez-Calvo, Manuel Vázquez-Carrera, Luis Masana, Dietbert Neumann

“…AMPK activation by AICAR was confirmed by increased phospho-Thr172-AMPK and phospho-Ser79-ACC protein levels in HL-1 cardiomyocytes. Then, cells were exposed to AICAR stimulation for 24 h in the presence or absence of the AMPK inhibitor Compound C, and the mRNA levels of the three PPARs were analyzed by real-time RT-PCR. …”
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Heterogeneous focal adhesion cytoskeleton nanoarchitectures from microengineered interfacial curvature to oversee nuclear remodeling and mechanotransduction of mesenchymal stem cel... by Huayu Fan, Hui Zhao, Yan Hou, Danni Meng, Jizong Jiang, Eon-Bee Lee, Yinzheng Fu, Xiangdong Zhang, Rui Chen, Yongtao Wang

“…Nuclear force-sensing mechanotransduction was explained by yes-associated protein (YAP) and laminA/C analysis. Results The micropatterned colony of hMSCs demonstrated the coincident characters with engineered micropores of microstencils. …”
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A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis by Ping Ma, Shiyao Kang, Huimin Li, Ming Li, Yuan Zhao, Hongjun Yuan, Jianyu Pang, Wenru Tang, Miaomiao Sheng

“…Further study of the mechanisms underlying these effects revealed that AC112721.1 regulates the Ras pathway by directly binding to THBS1 protein and functions as a ceRNA by sponging miR-491-5p to increase the expression of C2CD2L, thereby influencing the progression of TNBC. …”
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Chronic Glucocorticoid-Rich Milieu and Liver Dysfunction by Hernán Gonzalo Villagarcía, Vanesa Sabugo, María Cecilia Castro, Guillermo Schinella, Daniel Castrogiovanni, Eduardo Spinedi, María Laura Massa, Flavio Francini

“…In liver we assessed (a) OS (GSH and protein carbonyl groups) and inflammatory (IL-1b, TNFa, and PAI-1) biomarkers and (b) carbohydrate and lipid metabolisms. …”
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Recent Advances in Biotechnological Methods for Wheat Gluten Immunotoxicity Abolishment – a Review by Vijole Bradauskiene, Lina Vaiciulyte-Funk, Bakht Ramin Shah, Darius Cernauskas, Mihaela Adriana Tita

“…Some of the enzymes hydrolyze gluten, others block the action of toxic protein fragments. The majority of studies were carried out using lactic acid bacteria cultures, as single strains or in consortia. …”
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Decursinol angelate relieves inflammatory bowel disease by inhibiting the ROS/TXNIP/NLRP3 pathway and pyroptosis by Yudi Wang, Yudi Wang, Jiamin Wang, Yonghu Chen, Xuezheng Li, Xuezheng Li, Zhe Jiang, Zhe Jiang

“…Enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression levels of pro-inflammatory cytokines in mouse serum, Western blot was performed to detect the expression of TXNIP/NLRP3 pathway tight junction (TJ) proteins in colon tissues and cells, and immunohistochemistry, immunofluorescence and immunohistochemistry, immunofluorescence and qRT-PCR were used to validate the proteins related to this signaling pathway. …”
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Formononetin ameliorates polycystic ovary syndrome through suppressing NLRP3 inflammasome by Zhuo Liu, Rui-Han Wang, Ke-Hua Wang

“…Besides, the function of FMN on cell viability and apoptosis in DHT-induced PCOS cell model were explored by using CCK-8 assay and flow cytometry. Protein expression was detected via western blot and immunofluorescence staining in the DHEA-induced PCOS rat model and DHT-induced PCOS cell model. …”
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