In vivo antitumor activity of PHT-427 inhibitor-loaded polymeric nanoparticles in head and neck squamous cell carcinoma by Joaquín Yanes-Díaz, Raquel Palao-Suay, Francisca Inmaculada Camacho-Castañeda, Juan Riestra-Ayora, María Rosa Aguilar, Ricardo Sanz-Fernández, Carolina Sánchez-Rodríguez

“…In addition, we measured PI3KCA/AKT/PDK1 gene expression, PI3KCA/AKT/PDK1 protein levels, Epidermal Growth Factor Receptor (EGFR), and angiogenesis in the tumor tissue. …”
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Regulation of ER stress-induced apoptotic and inflammatory responses via YAP/TAZ-mediated control of the TRAIL-R2/DR5 signaling pathway by Y. El Yousfi, F. J. Fernández-Farrán, F. J. Oliver, A. López-Rivas, R. Yerbes

“…Mechanical signals emerging from extracellular matrix (ECM) rigidity and cell shape regulate the activity of transcriptional co-activators Yes-associated protein (YAP) and its paralog Transcriptional Coactivator with PDZ-binding motif (TAZ). …”
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Negative Effects of High Glucose Exposure in Human Gonadotropin-Releasing Hormone Neurons by Annamaria Morelli, Paolo Comeglio, Erica Sarchielli, Ilaria Cellai, Linda Vignozzi, Gabriella B. Vannelli, Mario Maggi

“…High glucose exposure (22 mM; 40 mM) significantly reduced gene and protein expression of GnRH, KISS1R, KISS1, and leptin receptor, as compared to normal glucose (5 mM). …”
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Pancreatic Cancer: Molecular, Biochemical, Chemopreventive, and Therapeutic Aspects by Juan Iovanna, José Luis Neira

“…The inhibition of signal pathways can be carried out not only by small molecules, able to bind to selected regions of the target protein, but also by using large molecules as antibodies. …”
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Analysis of expression and transport activity of sugar transporter gene HpSWEET7 from red pitaya by ZHENG Qianming, YAN Shuang, XIE Pu, WANG Honglin, MA Yuhua

“…Gene expression was analyzed by qRT-PCR, and the subcellular localization was detected by fusing fluorescent protein. Sugar transport activity was investigated by the yeast growth complementation and acidification assay. …”
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Research note: Protective effects of compound mycotoxin detoxifier in broiler chicken fed aflatoxins-contaminated diets by Kourosh Gholami Ahmadabadi, Mahsa Rahimi, Hamid Raei, Reza Majedifar, Mohammad Amir Karimi Torshizi

“…Among biochemistry parameters, total protein, LDL, and uric acid decreased in LCTB compared to the PC (P < 0.05). …”
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Alpha-Linolenic Acid Attenuates Inflammatory Response in LPS-stimulated Macrophages by Regulating TLR4/MyD88/NF-κB Signaling Pathway by Ruixin CAI, Xiaodie CHEN, Haibin ZHU, Yushun QIAN, Jianhua XIE, Mingyue SHEN

“…The levels of monocyte chemotactic protein (MCP-1), interleukin4 (IL-4), interleukin13 (IL-13), the production of reactive oxygen species (ROS) and the expression of related proteins in inflammatory signaling pathways were determined to explore the mechanism of α-linolenic acid in alleviating LPS-induced inflammatory response. …”
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Prebreeding studies of leaf rust resistant <i>Triticum aestivum/T. timopheevii</i> line L624 by S. N. Sibikeev, I. G. Adonina, A. E. Druzhin, O. A. Baranova

“…Introgressions from T. timopheevii in L624 increased the grain protein content by comparison with Saratovskaya 68 and Favorit, but it was at the same level as in Dobrynya. …”
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Simultaneously delivery of functional gallium ions and hydrogen sulfide to endow potentiated treatment efficacy in chemo- and PARPi-resistant ovarian cancer by Sangsang Tang, Yangyang Li, Yifeng Fang, Mengyan Tu, Shenglong Wu, Yixuan Cen, Junfen Xu

“…Meanwhile, Ga2S3-BSA NMs significantly inhibited NF-κB signaling and Bcl2 protein expression, leading to cell apoptosis. Furthermore, Ga2S3-BSA NMs increased cellular lipid peroxidation and triggered ferroptosis. …”
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Curcumin reverses cognitive deficits through promoting neurogenesis and synapse plasticity via the upregulation of PSD95 and BDNF in mice by Gaifen Li, Qiong Wu, Chao Wang, Pin Deng, Jiaxin Li, Zhiguang Zhai, Yubo Li

“…At the molecular level, curcumin was found to promote the expression of brain-derived neurotrophic factor (BDNF) and postsynaptic density protein 95 (PSD95). The results of primary hippocampal neuron detection showed that curcumin could promote dendritic growth. …”
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The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways by Jintao Wu, Na Li, Yuan Fan, Yanqiu Wang, Yongchun Gu, Zehan Li, Yin Pan, Gobin Romila, Zuomin Zhou, Jinhua Yu

“…CTP-CM had no significant effect on the proliferation of hDPSCs as indicated by CCK-8 and FCM analysis. Both the gene and protein (DSPP/DSPP, RUNX2/RUNX2, OCN/OCN, OSX/OSX, OPN/OPN, ALP/ALP, and COL-1/COL-1) expression levels increased in the CTP-CM-induced hDPSC group as compared with those in the control group at day 3 or 7, showing the positive regulation of CTP-CM on the osteo/odontogenic differentiation of hDPSCs. …”
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Addressing genome scale design tradeoffs in Pseudomonas putida for bioconversion of an aromatic carbon source by Deepanwita Banerjee, Javier Menasalvas, Yan Chen, Jennifer W. Gin, Edward E. K. Baidoo, Christopher J. Petzold, Thomas Eng, Aindrila Mukhopadhyay

“…While a complete implementation of the design was achieved, this study highlights the challenge of completely inactivating metabolic reactions encoded by under-characterized proteins, especially in the context of multi-gene edits.…”
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Recombinant production platform for Group A Streptococcus glycoconjugate vaccines by Sowmya Ajay Castro, Ian J. Passmore, Didier Ndeh, Helen Alexandra Shaw, Alessandro Ruda, Keira Burns, Sarah Thomson, Rupa Nagar, Kathirvel Alagesan, Mark Reglinski, Kieron Lucas, Sherif Abouelhadid, Ulrich Schwarz-Linek, Fatme Mawas, Göran Widmalm, Brendan W. Wren, Helge C. Dorfmueller

“…RhaPS is a validated universal vaccine candidate in a glycoconjugate prepared by chemical conjugation of the native carbohydrate to a carrier protein. We engineered the Group A Carbohydrate biosynthesis pathway to enable recombinant production using the industry standard route to couple RhaPS to selected carrier proteins within Escherichia coli cells. …”
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The Impact of Obstructive Sleep Apnoea and Nasal Continuous Positive Airway Pressure on Circulating Ischaemia-Modified Albumin Concentrations by Firat Uygur, Hakan Tanriverdi, Murat Can, Tacettin Ornek, Fatma Erboy, Bulent Altinsoy, Figen Atalay, Murat Damar, Furuzan Kokturk, Meltem Tor

“…Serum IMA concentrations correlated significantly with the apnoea-hypopnoea index, mean SaO2, desaturation index, and C-reactive protein concentrations. Multiple logistic regression analyses showed that OSAS increased the serum IMA concentration independent of age, sex, body mass index, smoking habit, and cardiovascular disease. …”
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Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages by Laura Hyväri, Miina Ojansivu, Miia Juntunen, Kimmo Kartasalo, Susanna Miettinen, Sari Vanhatupa

“…This study examined the significance of focal adhesion kinase (FAK), Rho-Rho-associated protein kinase (Rho-ROCK), and their downstream target extracellular signal-regulated kinase 1/2 (ERK1/2) on hASCs differentiation towards osteoblasts and adipocytes. …”
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Neural Mobilization Treatment Decreases Glial Cells and Brain-Derived Neurotrophic Factor Expression in the Central Nervous System in Rats with Neuropathic Pain Induced by CCI in R... by Aline Carolina Giardini, Fabio Martinez dos Santos, Joyce Teixeira da Silva, Mara Evany de Oliveira, Daniel Oliveira Martins, Marucia Chacur

“…Thalamus and midbrain were analyzed for glial fibrillary acidic protein (GFAP), microglial cell OX-42, and BDNF using Immunohistochemistry and Western blot assays. …”
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Tetrahydrobiopterin as a rheostat of cell resistance to oxidant injury by R. Steven Traeger, James Woodcock, Sidhartha Tan, Zhongjie Shi, Jeannette Vasquez-Vivar

“…Following the activation of peroxynitrite production with PMA in lipopolysaccharide-treated cells, we also found a significant time-dependent decline in GTPCH-I protein levels. We conclude that hydrogen peroxide is the least effective oxidant species at decreasing intracellular BH4 levels, while peroxynitrite is highly effective by targeting GTPCH-I and BH4 directly. …”
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Proteostasis and Mitochondrial Role on Psychiatric and Neurodegenerative Disorders: Current Perspectives by Pablo Olivero, Carlo Lozano, Ramón Sotomayor-Zárate, Nicolás Meza-Concha, Marcelo Arancibia, Claudio Córdova, Wilfredo González-Arriagada, Ricardo Ramírez-Barrantes, Ivanny Marchant

“…Proteostasis involves processes that are fundamental for neural viability. Thus, protein misfolding and the formation of toxic aggregates at neural level, secondary to dysregulation of the conservative mechanisms of proteostasis, are associated with several neuropsychiatric conditions. …”
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Role of CXCL10 released from osteocytes in response to TNF-α stimulation on osteoclasts by Mariko Miura, Hideki Kitaura, Fumitoshi Ohori, Kohei Narita, Jiayi Ren, Takahiro Noguchi, Aseel Marahleh, Jinghan Ma, Angyi Lin, Ziqiu Fan, Itaru Mizoguchi

“…In this study, highly purified osteocytes were isolated from dentin matrix protein 1 (DMP1)-Topaz mice using cell sorter. RNA sequencing (RNA-seq) revealed that TNF-α stimulation increased C-X-C motif chemokine ligand 10 (CXCL10) gene expression in osteocytes. …”
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PAC-Mediated AKI Protection Is Critically Mediated but Does Not Exclusively Depend on Cell-Derived Microvesicles by H. Dihazi, K. Schwarze, S. Patschan, G. A. Müller, O. Ritter, M. Zeisberg, D. Patschan

“…Syngeneic murine PACs were stimulated with either melatonin, angiopoietin-1 or -2, or with bone morphogenetic protein-5 (BMP-5) for one hour, respectively. PAC-derived MV and the vesicle-depleted supernatant were subsequently collected and i.v.…”
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