The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes by Di Cong, Xue Zhao, Xue Zhao, Chang Ni, Mengru Li, Luwen Han, Jianlin Cheng, Hongzhang Liu, Huijing Liu, Dan Yao, Shuying Liu, Guoshuang Chen

“…In addition, subcellular localization experiments showed that the HrSEP1 protein was localized in the nucleus. We verified the interaction of HrSEP1 with HrSOC1, HrSVP, and HrAP1 using yeast two-hybrid and bimolecular fluorescence complementation assays. …”
Get full text

Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis by Christiane Radzimski, Christian Probst, Bianca Teegen, Kristin Rentzsch, Inga Madeleine Blöcker, Cornelia Dähnrich, Wolfgang Schlumberger, Winfried Stöcker, Dimitrios P. Bogdanos, Lars Komorowski

“…In contrast to the determination of other AIH-associated autoantibodies by indirect immunofluorescence assay (IFA), detection of anti-SLA relied up to now on ELISA or immunoblot based on bacterially expressed recombinant protein. In order to develop a complementary IFA substrate, SLA isoform 1 was recombinantly produced in the human cell line HEK293 and controlled by a rabbit hyperimmune serum against SLA. …”
Get full text

Qingfei Tongluo Mixture Attenuates Bleomycin-Induced Pulmonary Inflammation and Fibrosis through mTOR-Dependent Autophagy in Rats by Shuyu Ge, Zhenghong Guo, Ting Xiao, Pingping Sun, Bo Yang, Yin Ying

“…The expression changes of pulmonary fibrosis and autophagy marker proteins are attenuated by QFTL. Furthermore, the autophagy inhibitor CQ significantly reversed the downward trend in HYP levels and α-SMA protein expression, which QFTL improved in BLM-induced pulmonary fibrosis rats. …”
Get full text

A guide to selecting high-performing antibodies for Huntingtin (UniProt ID: P42858) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2... by Rachel J. Harding, Carl Laflamme, Sara González Bolívar, Aled M. Edwards, Kathleen Southern, Rebeka Fanti, Riham Ayoubi, Charles Alende, Maryam Fotouhi, Renu Chandrasekaran

“…Huntingtin encodes a 3144 amino acid protein, with a polyglutamine repeat tract at the N-terminus. …”
Get full text

Genes related to the white face colour pattern in eight Russian cattle breeds by N. S. Yudin, N. M. Belonogova, D. M. Larkin

“…SLC41A2 encodes a magnesium transporter protein. However, the function of this gene is not well established. …”
Get full text

Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation by Huixia Qiao, Yahui Huang, Xiaoyan Chen, Long Yang, Yue Wang, Rongrong Xu

“…JWSYD suppressed the protein levels of inflammasome-associated proteins, including NLRP3, ASC1, Procaspase-1, Cleaved caspase-1, and Cleaved IL-1β in DSS-induced UC mice and LPS-induced RAW264.7 cells. …”
Get full text

Lipid-Derived Mediators in Endogenous Anti-Inflammation and Resolution: Lipoxins and Aspirin-Triggered 15-epi-Lipoxins by Charles N. Serhan, Nan Chiang

“…Both LXA4 and 15-epi-LXA4 elicit these responses via a G protein-coupled receptor (GPCR), termed ALXR, identified in human and murine tissues. …”
Get full text

Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II by Cagri Sakalar, Suparna Mazumder, Justin M. Johnson, Cengiz Z. Altuntas, Ritika Jaini, Robert Aguilar, Sathyamangla V. Naga Prasad, Denise C. Connolly, Vincent K. Tuohy

“…Anti-Müllerian hormone receptor, type II (AMHR2), is a differentiation protein expressed in 90% of primary epithelial ovarian carcinomas (EOCs), the most deadly gynecologic malignancy. …”
Get full text

TNRC6C Functions as a Tumor Suppressor and Is Frequently Downregulated in Papillary Thyroid Cancer by Zhenqin Cai, Tianyu Zhai, Dilidaer Muhanhali, Yan Ling

“…Immunohistochemical assay was performed to detect the protein expression of TNRC6C in PTC and its adjacent noncancerous tissue. …”
Get full text

Squamate reptiles may have compensated for the lack of γδTCR with a duplication of the TRB locus by Jordan M. Sampson, Kimberly A. Morrissey, Kieran J. Mikolajova, Kourtney M. Zimmerly, Neil J. Gemmell, Michael G. Gardner, Terry Bertozzi, Terry Bertozzi, Robert D. Miller

“…Based on conserved residues, the predicted protein chain would be expected to form a heterodimer with TCRα. …”
Get full text

Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum by Xiang Chen, Qiyang Zhou, Ting Zhang, ChunXin Wang, Zheng Yu, Hadji Ahamada, Zhonghu Bai, Xuan Huang

“…Normally, FA molecules bind to folate-binding protein (FBP) in the serum as complex. Before quantify the FA concentration, a releasing procedure should be conducted. …”
Get full text

Effects of prophylactic antibiotic administration and antibiotic timing on culture results and clinical outcomes of paediatric musculoskeletal infection: a protocol for a randomise... by Chao You, Yibiao Zhou, Zhiyong Liu, Chao Deng, Yongjie Xia, Dechao Wu, Liangfu Xie, Bing E, Jingming Han

“…In addition, the disease-related markers including white blood cell count, C reactive protein, erythrocyte sedimentation rate, vital signs as well as the length of hospital stay will be measured or recorded accordingly. …”
Get full text

Expression of SIRT1 and DBC1 in Developing and Adult Retinas by Shawn C. Maloney, Emilia Antecka, Alexandre N. Odashiro, Bruno F. Fernandes, Madeline Doyle, Li-Anne Lim, Yousef Katib, Miguel N. Burnier

“…We found both SIRT1 and DBC1 to be widely expressed in mouse and human retinas, with subtle differences in subcellular distribution of each protein. We further demonstrate that nuclear-localized SIRT1 is only seen in human-derived retinal progenitor cells and not in adult retinas, suggesting that this nuclear localization may be important in retinal development. …”
Get full text

Assessment of derangement in biochemical profile in primary open angle glaucoma patients presenting at a tertiary care hospital by Fatima Tuz Zuhra, Mehnaz Khattak, Sana Nadeem, Noreen Atzaz, Attika Khalid, Sami Saeed

“… Objective: To assess the changes in high sensitivity C Reactive protein (hs-CRP), serum cholesterol, uric acid, creatinine, bilirubin and ALT in patients of primary open angle glaucoma. …”
Get full text

Advancing Therapeutic Targets in IBD: Emerging Goals and Precision Medicine Approaches by Lucia Centanni, Clelia Cicerone, Fabrizio Fanizzi, Ferdinando D’Amico, Federica Furfaro, Alessandra Zilli, Tommaso Lorenzo Parigi, Laurent Peyrin-Biroulet, Silvio Danese, Mariangela Allocca

“…Key targets now include clinical remission, endoscopic remission, and biomarker normalization (such as C-reactive protein and fecal calprotectin). Moreover, histologic remission, transmural remission, and in the future molecular targets are emerging as important indicators of sustained disease control. …”
Get full text

Long noncoding RNA GDIL acts as a scaffold for CHAC1 and XRN2 to promote platinum resistance of colorectal cancer through inhibition of glutathione degradation by Xuan Deng, Lu Chang, Lingyu Tang, Haoqin Jiang, Xiao Xu, Xinju Zhang, Jian Chen, Liu Dong, Qianqian Xu, Ruoshui Cao, Jianbin Xiang, Ming Guan

“…Mechanistically, GDIL binds and re-localizes the nuclear protein XRN2 to the cytoplasm, where GDIL further serve as a scaffold for XRN2 to identify and degrade CHAC1 mRNA. …”
Get full text

Advanced progress in the genetic modification of the oncolytic HSV-1 virus by Mi Zhou, Mi Zhou, Mi Zhou, Zhenyu Shen, Zhenyu Shen, Zhenyu Shen

“…The HSV-1 genome can be modified through genetic engineering in two main ways: first, by reducing infectivity and toxicity to normal cells via limited replication and assembly, altered protein-virus receptor binding, and minimized immune evasion; second, by enhancing anticancer activity through disruption of tumor cell metabolism, induction of autophagy, improved immune recognition, and modification of the tumor microenvironment. …”
Get full text

Therapy of Prostate Cancer by Nanoyam Polysaccharide by Cheng Peng, Bo Han, Baishan Wang, Gaoyang Zhao, Fang Yuan, Yeyu Zhao, Liang Liang, Dingge Liu, Zhaohui Zhai, Yanping Shen

“…The levels of caspase-3 protein were determined with Western blot. In our data, nanoyam polysaccharide presented inhibitory effect on the proliferation of PC-3. …”
Get full text

Review: Feed efficiency and metabolic flexibility in livestock by W.M. Rauw, L.H. Baumgard, J.C.M. Dekkers

“…We start with a brief overview of energy processes and substrate metabolism of carbohydrates, lipids, and protein. During muscle metabolism, the type of fuel used depends on fibre type characteristics of the muscle. …”
Get full text

Expression and prognosis of cellular senescence genes in head and neck squamous cell carcinoma by Chen Feng, Lijuan Zhu, Wei Mao, Pin Dong, Xinwei Chen

“…We used the HPA database for protein-level validation of the genes. Results We identified 5 cellular senescence genes associated with HNSC, namely, BTG3, EHF, EZH2, TACC3 and TXN. …”
Get full text