Protocol for evaluating the activity of R2 retrotransposons in mammalian cells by Yangcan Chen, Shengqiu Luo, Yanping Hu, Qi Zhou, Wei Li

“…We describe the construction of vectors separately expressing R2 protein and donor, the process of liposome transfection, and flow cytometry. …”
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Effect of epigallocatechin-3-gallate (EGCG) on cognitive functioning and the expression of APP and BDNF in the hippocampus of rats with streptozotocin -induced Alzheimer-like disea... by Farnaz Ghayour Babaei, Ehsan Saburi, Fatemeh Forouzanfar, Mohadese Asgari, Zakieh Keshavarzi, Vahid Hajali

“…Real-time PCR assay was used for evaluating the expression of beta-amyloid precursor protein (APP) and brain-derived neurotrophic factor (BDNF) in the hippocampus of animals. …”
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The production process of chepa shutki: A traditional Bangladeshi fermented fish product by Jasmin Ara Farhana, Sijmen E. Schoustra, Ferdous Ahamed, Bas Zwaan, Oscar van Mastrigt

“…Although chepa shutki is an important part of the human diet in Bangladesh, providing flavour as well as essential nutrients (especially protein and bone minerals), research on variations in production of chepa shutki has been limited. …”
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Understanding variation in management of early-onset neonatal sepsis in India: a vignette-based survey by Christina M Schumacher, Samuel McAleese, Tushar B Parikh, Basma Ouddi, Julia Johnson

“…Screening tests such as complete blood count and C reactive protein were used in 56%–67% of cases, and abnormal results led to longer courses with fewer than 10% of respondents discontinuing antibiotics by 72 hours. …”
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A dynamic co-expression approach reveals Gins2 as a potential upstream modulator of HNSCC metastasis by Nasibeh Khayer, Samira Shabani, Maryam Jalessi, Mohammad Taghi Joghataei, Frouzandeh Mahjoubi

“…Finally, the validity of biologically relevant triplets was assessed at the protein level. The results highlighted the “PI3K/AKT/mTOR (PAM) signaling pathway” as a disrupted pathway involved in the metastatic nature of HNSCC. …”
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Successful Tocilizumab Therapy for Macrophage Activation Syndrome Associated with Adult-Onset Still’s Disease: A Case-Based Review by Eri Watanabe, Hitoshi Sugawara, Takeshi Yamashita, Akira Ishii, Aya Oda, Chihiro Terai

“…Soon afterward, her fever ceased and high levels of both ferritin and C-reactive protein levels decreased. Her course was complicated by disseminated intravascular coagulation, cytomegalovirus infection, and Pneumocystis jirovecii pneumonia. …”
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Rheumatoid factor isotypes in rheumatoid arthritis diagnosis and prognosis: a systematic review and meta-analysis by Nicola Bizzaro, Franco Franceschini, Maria Infantino, Carlo Selmi, Gian Domenico Sebastiani, Francesca Motta, Davide Giavarina, Boaz Palterer

“…When analysing anti-citrullinated protein antibodies, RF isotype determination increases diagnostic accuracy. …”
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Plasma brain-related biomarkers and potential therapeutic targets in pediatric ECMO by Sue J. Hong, Bradley J. De Souza, Kristen K. Penberthy, Lisa Hwang, David E. Procaccini, John N. Kheir, Melania M. Bembea

“…Central nervous system (CNS) neuronal and astroglial cellular-derived neuron-specific enolase (NSE), tau, glial fibrillary acidic protein (GFAP) and S100β elevations have been detected in ABI and are associated with poorer outcomes. …”
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Mechanism of sensor kinase CitA transmembrane signaling by Xizhou Cecily Zhang, Kai Xue, Michele Salvi, Benjamin Schomburg, Jonas Mehrens, Karin Giller, Marius Stopp, Siegfried Weisenburger, Daniel Böning, Vahid Sandoghdar, Gottfried Unden, Stefan Becker, Loren B. Andreas, Christian Griesinger

“…An anti-parallel to parallel transition upon citrate binding agrees with interdimer distances measured in the lipid embedded protein using a site-specific 19F label in PASc. These data show how Angstrom scale structural changes in the sensor domain are transmitted across the membrane to be converted and amplified into a nm scale shift in the linker to the phosphorylation subdomain of the kinase.…”
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Effects of Polygonatum sibiricum Polysaccharides (PSP) on Human Esophageal Squamous Cell Carcinoma (ESCC) via NF-κB Signaling Pathway by Weizheng Zhou, Jiang Hong, Ji Zhu, Xiaowei Wang

“…Compared with the control group, the expression level of TLR4, NF-κB/p50, and NF-κB/p65 protein in each experimental group was significantly lower than that in the control group (p<0.05). …”
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The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey by N.D. Meads, R. Tahmasbi, N. Jantasila

“…The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. …”
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Comparison of CRISPR-Cas13b RNA base editing approaches for USH2A-associated inherited retinal degeneration by Lewis E. Fry, Lauren Major, Ahmed Salman, Lucy A. McDermott, Jun Yang, Andrew J. King, Michelle E. McClements, Robert E. MacLaren

“…In mice injected with PspCas13b constructs, usherin protein was successfully restored and correctly localized to the connecting cilium following RNA editing. …”
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Overexpression of StBBX14 Enhances Cold Tolerance in Potato by Heng Zhang, Mingjun Chen, Xiaobo Luo, Li Song, Fei Li

“…The subcellular localization of the StBBX14 protein took place in the nucleus. The <i>OE-StBBX14</i> transgenic lines showed less leaf damage and significantly lower electrolyte leakage compared with the WT under cold stress, indicating that the overexpression of <i>StBBX14</i> in the potato enhanced the cold resistance. …”
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Using Soluble Transferrin Receptor and Taking Inflammation into Account When Defining Serum Ferritin Cutoffs Improved the Diagnosis of Iron Deficiency in a Group of Canadian Presch... by Huguette Turgeon O’Brien, Rosanne Blanchet, Doris Gagné, Julie Lauzière, Carole Vézina

“…Iron depletion was defined as follows: (1) SF < 15 μg/L regardless of the C-reactive protein (CRP) level and (2) SF < 15 or <50 μg/L with CRP ≤ 5 or >5 mg/L, respectively. …”
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大鼠雪旺细胞对视网膜神经细胞成活 及突起生长的影响 by 杨智宽, 葛　坚, 刘海泉, 郭　彦

“…【方法】取出生 3～ 5 d 的 SpragueDaw ley 乳鼠的视网膜组织及坐骨神经, 用酶消化法分别得到视网膜神经细胞和雪旺细胞, 分别和联合接种于多聚赖氨酸包被 的培养皿、用含体积分数为 20%胎牛血清( fetal calf serum, FCS)的 BME( basal medium eagle)培养液进行培养, 倒置相差显微 镜观察细胞的生长情况; 视网膜神经细胞和胶质细胞分别用神经丝蛋白( neurofilament, NF)和胶质纤维酸性蛋白( glial fibrillary acidic protein, GFAP)进行鉴定,神经节细胞用 Thy1. 1 鉴定, 雪旺细胞培养时用阿糖胞苷进行纯化并用 S-100 鉴定, 扫描 电镜观察共生培养的细胞。…”
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Tillage and residue management modulate the links between soil physical signatures and arbuscular mycorrhizal fungal biomarkers by Stamatios Thomopoulos, Lars Juhl Munkholm, Lars Elsgaard, Sabine Ravnskov

“…Measurements included three soil biological indicators—two fatty-acid biomarkers of arbuscular mycorrhizal fungi (AMF) and easily extractable glomalin-related soil protein (EE) − as well as two soil physical indices (water-stable aggregates (WSA), and clay dispersibility), five soil pore characteristics (air permeability, gas diffusivity, tortuosity, total porosity, air-filled porosity, and volumetric water content) and soil organic carbon (SOC). …”
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Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse by Lisi Deng, Guangze Liu, Xin Wu, Yaping Wang, Minghua Tong, Bing Liu, Kuidong Wang, Yuting Peng, Xiangping Kong

“…ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. …”
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Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration by Pandi Chen, Gengfan Ye, Jia Li, Kuan Feng, Guangyao Zhu, Maosong Chen, Wei Chen

“…Subsequently, mRNA-transcription factor (mRNA-TF), mRNA–microRNA (mRNA–miRNA), mRNA-RNA-binding protein (mRNA-RBP), and mRNA-drug regulatory networks were established, followed by immune infiltration analysis. …”
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Determination of the physicochemical characteristics and bioactive compounds of the miracle fruit (Synsepalum dulcificum) considering different extraction and preservation methods by Juan Alejandro Neira Mosquera, Sungey Naynee Sánchez Llaguno, Karol Yannela Revilla Escobar, Jhonnatan Placido Aldas Morejon, Angie Estefania Iguasnia Ureta, Andy Sebastian Parrales Loor, Jonathan Alexander Arguello Cedeño

“…Utilizing a Completely Randomized Block Design with a factorial arrangement (A∗B), where factor A represented extraction methods (cold pressing, maceration, and pressurized liquids) and factor B represented preservation methods (fluid extract and ionic emulsion), the research found that bromatological analyses showed pH values ranging from 2.990 to 3.535, acidity between 0.13 and 0.14, soluble solids from 4.45 to 41.00, and protein content from 0.029 to 4.670. The maceration with fluid extract method achieved the most favorable results in terms of bioactive compounds, including flavonoids (20.14 mg Eq/g), anthocyanins (9.55 mg/g), total phenolic content (16.53 mg gallic acid/g), and antioxidant capacity (521.24 μg Trolox/g). …”
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Similarities and Differences between Coronavirus Disease 2019-related Multisystem Inflammatory Syndrome in Children and Kawasaki Disease in Indian Patients by Utkarsh Singh, Suhas Prabhu, Deepak Phalgune

“…Liver function tests (LFTs), renal function tests (RFTs), and inflammatory markers such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were comparable between the two groups. …”
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