O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139 by Adisak Bhumiratana, Achiraya Siriphap, Nutsarin Khamsuwan, Jednipit Borthong, Kaknokrat Chonsin, Orasa Sutheinkul

“…A panel of molecular marker-based TMPCR method generates reproducible profiles of V. cholerae-specific (588 bp) amplicons derived from ompW gene encoding the outer membrane protein and serogroup-specific amplicons, 364 bp for the O1 and 256 bp for the O139, authentically copied from rfb genes responsible for the lipopolysaccharide biosynthesis. …”
Get full text

Polyphenols of <i>Perilla frutescens</i> of the family Lamiaceae identified by tandem mass spectrometry by M. P. Razgonova, N. G. Kon’kova, A. M. Zakharenko, K. S. Golokhvast

“…Perilla seeds contain 40–53 % of oil, 28 % of protein. The growing season is 100–150 days. In Russia, perilla is grown in the Far East, where the yield is 0.8–1.2 t/ha. …”
Get full text

Milk production and nitrogen excretion of grazed dairy cows in response to plantain (Plantago lanceolata) content and lactation season by Thi Truong Nguyen, Soledad Navarrete, David Horne, Daniel Donaghy, Peter Kemp

“…Results The pasture treatments did not affect milk production, the yield and composition of milk solids, protein, fat, and lactose. However, cows grazing pastures containing between 17% and 28% dietary plantain reduced UN concentration by 15% to 27%, decreased UN excretion by 4% to 9%, and increased urine volume by 22% to 40%, compared to grazing the RGWC pasture. …”
Get full text

RBM47 is a novel immunotherapeutic target and prognostic biomarker in gliomas by Wei Wei, Yongfu Cao, Xin Lu, Long Wang, Jianbin Li, Guojun Deng, Donghai Li, Limin Xiao

“…Abstract The role of RBM47, an RNA-binding protein, in shaping the immune landscape of gliomas and tumor immune responses is yet to be fully studied. …”
Get full text

Disease Stage-Associated Alterations in Learning and Memory through the Electroacupuncture Modulation of the Cortical Microglial M1/M2 Polarization in Mice with Alzheimer’s Disease... by Long Li, Le Li, Jiayong Zhang, Sheng Huang, Weilin Liu, Zhifu Wang, Shengxiang Liang, Jing Tao, Lidian Chen

“…In this study, we demonstrated that electroacupuncture at the Shenting and Baihui acupoints for 16 weeks could improve learning and memory in the Morris water maze test and reduce amyloid β-protein in the parietal association cortex and entorhinal cortex in mice with mild and moderate AD. …”
Get full text

PIM2 inhibition promotes MCL1 dependency in plasma cells involving integrated stress response-driven NOXA expression by Marion Haas, Sabrina Cherfa, Léa Nguyen, Maxence Bourgoin, Gersende Caron, Elise Dessauge, Tony Marchand, Laurent Delpy, Patrick Auberger, Jérôme Moreaux, Arnaud Jacquel, Thierry Fest

“…Abstract Our study explores the complex dynamics of the integrated stress response (ISR) axis, highlighting PIM2 kinase’s critical role and its interaction with the BCL2 protein family, uncovering key mechanisms of cell survival and tumor progression. …”
Get full text

MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue by Tung-Yueh Chuang, Hsiao-Li Wu, Chen-Chun Chen, Gloria Mabel Gamboa, Lawrence C. Layman, Michael P. Diamond, Ricardo Azziz, Yen-Hao Chen

“…Unlike what is reported in cardiomyocytes, overexpression of miR-223 in human differentiated adipocytes was associated with a reduction in GLUT4 protein content and insulin-stimulated glucose uptake. …”
Get full text

A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy by Andreas Schulte, Janik Brockmann, Nina Müller, Tibor Anderlei, Jochen Büchs

“…Physiological states, such as growth vs. protein production, could be revealed, and quantitative analysis of metabolites was performed, putting focus on RQ measurements. …”
Get full text

Persistence with Early-Line Abatacept versus Tumor Necrosis Factor-Inhibitors for Rheumatoid Arthritis Complicated by Poor Prognostic Factors by Xue Han, Francis Lobo, Michael S. Broder, Eunice Chang, Sarah N. Gibbs, David J. Ridley, Irina Yermilov

“…Poor prognostic factors included ACPA+, RF+, increased C-reactive protein levels, elevated erythrocyte sedimentation rate levels, or presence of joint erosions. …”
Get full text

Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice by Yi Chen, Qin Li, Zhiping Kuang, Xin Zhao, Ruokun Yi, Xiaowei He, Guangjing Feng

“…The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. …”
Get full text

Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated... by Yoko Harima, Takuro Ariga, Yuko Kaneyasu, Hitoshi Ikushima, Sunao Tokumaru, Shigetoshi Shimamoto, Takeo Takahashi, Noriko Ii, Kayoko Tsujino, Anneyuko I Saito, Hiroki Ushijima, Takafumi Toita, Tatsuya Ohno

“…In this multicenter prospective cohort study, the usefulness of pre- and posttreatment serum protein levels of potential biomarkers, including squamous cell carcinoma antigen (SCC-Ag), apolipoprotein C-II (ApoC-II), matrix metalloproteinase (MMP)1, and MMP2, were evaluated together with clinical factors in 145 cervical cancer patients in order to determine their suitability to predict survival. …”
Get full text

Assessment of Anti-Inflammatory and Antioxidant Activities of a Proprietary Preparation of Quercetin–Rutin Blend (SophorOx™) in Exercised Rats by Devanand Shanmugasundaram, James Martin Roza

“…In addition, serum collected on days 8, 15, 22, and 29 were measured for the liver function test (LFT), random blood sugar (RBS), inflammatory markers C-reactive protein (CRP), oxidative stress markers (8-isoprostane (8-iso-PGF2α), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and cytokine levels interleukin-1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α)) by the ELISA method. …”
Get full text

Effect of Dipeptidyl Peptidase-4 (DPP-4) Inhibition on Biomarkers of Kidney Injury and Vascular Calcification in Diabetic Kidney Disease: A Randomized Controlled Trial by Thananda Trakarnvanich, Bancha Satirapoj, Swangjit Suraamornkul, Thanit Chirananthavat, Anoma Sanpatchayapong, Torpong Claimon

“…However, biomarkers of vascular calcification, including serum bone alkaline phosphatase and kidney injury, including urine neutrophil gelatinase-associated lipocalin (NGAL)/Cr and urine liver fatty acid-binding protein (L-FABP)/Cr, were improved significantly in the gemigliptin treatment group compared with the control group. …”
Get full text

Genome-Wide Identification and Expression Profiling of the <i>SPL</i> Transcription Factor Family in Response to Abiotic Stress in Centipedegrass by Dandan Kong, Maotao Xu, Siyu Liu, Tianqi Liu, Boyang Liu, Xiaoyun Wang, Zhixiao Dong, Xiao Ma, Junming Zhao, Xiong Lei

“…SQUAMOSA promoter-binding protein-like (SPL) transcription factors play a critical role in the regulation of gene expression and are indispensable in orchestrating plant growth and development while also improving resistance to environmental stressors. …”
Get full text

Taurine Alleviated the Negative Effects of an Oxidized Lipid Diet on Growth Performance, Antioxidant Properties, and Muscle Quality of the Common Carp (Cyprinus carpio L.) by Dan Liu, Jiali Mi, Xiao Yan, Chaobin Qin, Junli Wang, Guoxing Nie

“…In addition, compared to the FO group, OFO fish showed an increased content of malondialdehyde and protein carbonylation and decreased hardness, brightness, pH, superoxide dismutase, and catalase levels in the muscle (P<0.05). …”
Get full text

Food Intake and Core Body Temperature of Pups and Adults in a db Mouse Line Deficient in the Long Form of the Leptin Receptor without Misty Mutation by Wijang Pralampita Pulong, Miharu Ushikai, Emi Arimura, Masaharu Abe, Hiroaki Kawaguchi, Masahisa Horiuchi

“…Additionally, db/db mice displayed significantly lower mRNA levels of brown adipose tissue uncoupling protein 1 at both ages. In conclusion, the db mouse line without the misty mutation clearly showed the different involvements of the Lepr long form in FI and BT in pups and adults.…”
Get full text

SOX7 is involved in polyphyllin D-induced G0/G1 cell cycle arrest through down-regulation of cyclin D1 by Zheng Bin, Wang Gang, Gao Wenbo, Wu Qiquan, Zhu Weizhi, Weng Guobin

“…Cell cycle progression was analyzed using propidium iodide (PI) staining, and protein levels were assayed by Western blot analysis. …”
Get full text

Effect of salt stress on plants of wild-type <i>Nicotiana tabacum</i> L. and transformants with a choline oxidase (<i>cod</i>A) gene by I. G. Shirokikh, S. Yu. Ogorodnikova, Ya. I. Nazarova, O. N. Shupletsova

“…It was shown that the transformation of plants with the bacterial gene of choline oxidase, followed by the accumulation of the protein product of the codA gene – glycine betaine, even in a minimal amount, was accompanied by positive effects on tobacco plants under salt stress conditions.…”
Get full text

Surface remodeling and inversion of cell-matrix interactions underlie community recognition and dispersal in Vibrio cholerae biofilms by Alexis Moreau, Danh T. Nguyen, Alexander J. Hinbest, Anthony Zamora, Ranjuna Weerasekera, Katherine Matej, Xuening Zhou, Sandra Sanchez, Ignacio Rodriguez Brenes, Jung-Shen Benny Tai, Carey D. Nadell, Wai-Leung Ng, Vernita Gordon, Natalia L. Komarova, Rich Olson, Ying Li, Jing Yan

“…We combine genetics, microscopy, simulations, and biochemical analyses to show that V. cholerae cells are not attracted to the main matrix component (Vibrio polysaccharide, VPS), but can be attached to each other and to the VPS network through surface-associated VPS and crosslinks formed by the protein Bap1. Downregulation of VPS production and surface trimming by the polysaccharide lyase RbmB cause surface remodeling as biofilms age, shifting the nature of cell-matrix interactions from attractive to repulsive and facilitating cell dispersal as aggregated groups. …”
Get full text

Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury by Khaled G. Abdel-Wahhab, Mahmoud Ashry, Laila K. Hassan, Marwa H. El-Azma, Ghada M. Elqattan, Mohamed H. A. Gadelmawla, Fathia A. Mannaa

“…Additionally, hepatic glutathione, Na+/K+-ATPase, and glutathione peroxidase, as well as serum HDL-C, total protein and albumin levels were significantly reduced. …”
Get full text