可溶性TAT PTD-Ngb融合蛋白的原核表达、鉴定与纯化及其对皮质神经元的跨膜转导 by 周国钰, 胡学强, 胡骏, 陆正齐, 楼之茵, 朱灿胜, 熊洁萍

“…【目的】构建含蛋白转导域（protein transduction domain,PTD）与脑红蛋白（neuroglobin,Ngb）融合基因的表达质粒,原核表达可溶性TATPTD-Ngb,并鉴定、纯化,检测TATPTD-Ngb对皮质神经元的跨膜转导功能。…”
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Structural insights into light-gating of potassium-selective channelrhodopsin by Takefumi Morizumi, Kyumhyuk Kim, Hai Li, Probal Nag, Tal Dogon, Oleg A. Sineshchekov, Yumei Wang, Leonid S. Brown, Songhwan Hwang, Han Sun, Ana-Nicoleta Bondar, Igor Schapiro, Elena G. Govorunova, John L. Spudich, Oliver P. Ernst

“…Here, we use single-particle cryo-electron microscopy of peptidisc-incorporated protein samples to determine the structures of the slow-cycling mutant C110A of kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) in the dark and upon laser flash excitation. …”
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(−)-Epigallocatechin-3-Gallate and Quercetin Inhibit Quiescin Sulfhydryl Oxidase 1 Secretion from Hepatocellular Carcinoma Cells by Lumin Yang, Yuying Fang, Yufeng He, Jinsong Zhang

“…Since (−)-epigallocatechin-3-gallate (EGCG) has been demonstrated to be an effective inhibitor of α-fetal protein secretion from HCC cells, we screened QSOX1 secretion inhibition using polyphenolic compounds. …”
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Mechanisms of multidrug resistance caused by an Ipi1 mutation in the fungal pathogen Candida glabrata by Taiga Miyazaki, Shintaro Shimamura, Yohsuke Nagayoshi, Hironobu Nakayama, Akihiro Morita, Yutaka Tanaka, Yasuhiko Matsumoto, Tatsuo Inamine, Hiroshi Nishikawa, Nana Nakada, Makoto Sumiyoshi, Tatsuro Hirayama, Shigeru Kohno, Hiroshi Mukae

“…The C. glabrata Ipi1 protein physically interacts with the ribosome-related chaperones Ssb and Ssz1, both of which bind to Pdr1. …”
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Effect of Different Feeding on Feed Conversion and Growth of Common Carp (Cyprinus carpio) in Floating Net Cage Culture at Jatiluhur Dike by N.B.P Utomo, F. Kumalasari, I. Mokoginta

“…Fish were fed on a commercial diet containing 30% protein at 5 times daily.  Experimental treatment was feeding technique, i.e., by 6% of body weight, and at satiation for the second treatment.  …”
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Sunflower lines originated from varieties with high oil content in their seed by V. A. Gavrilova, I. N. Anisimova, T. G. Stupnikova

“…Lines originated from one and the same variety can differ by the presence of diagnostic markers of the Rf1 gene controlling pollen fertility restoration in forms with cytoplasmic male sterility, and also by allelic variants of storage protein genes and morphological characters.…”
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Cancer-associated fibroblasts and their role in tumor progression by M. S. Ermakov, A. A. Nushtaeva, V. A. Richter, O. A. Koval

“…CAFs produce collagen, the main scaffold protein of the extracellular matrix. Collagen in the tumor stroma stimulates fibrosis, enhances the rigidity of tumor tissue, and disrupts the transmission of proliferation and differentiation signaling pathways. …”
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Lacticaseibacillus rhamnosus Fmb14 ameliorates hyperuricemia-induced hepatocyte pyroptosis via NLRP3 inflammasome cascade inhibition by Hongyuan Zhao, Xiaoyu Chen, Li Zhang, Fanqiang Meng, Libang Zhou, Zhaoxin Lu, Yingjian Lu

“…L. rhamnosus Fmb14 administration restored mitochondrial inner membrane protein (MPV17) and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice, indicating that dysbiosis of mitochondrial membrane potential was restored in liver. …”
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Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes (<i>Vitis vinifera</i> L.) Under Abiotic Stress and During Development by Xufeng Guo, Na He, Biying Huang, Chongyao Chen, Yanxia Zhang, Xiaoyu Yang, Jie Li, Zhigang Dong

“…Consequently, we conducted a comprehensive bioinformatics analysis of all <i>AAAP</i> genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. …”
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APOBEC3C‐mediated NF‐κB activation enhances clear cell renal cell carcinoma progression by Nora Hase, Danny Misiak, Helge Taubert, Stefan Hüttelmaier, Michael Gekle, Marcel Köhn

“…Here, we unveil that the enhanced expression of the RNA‐binding protein DNA dC → dU‐editing enzyme APOBEC‐3C (APOBEC3C; also known as A3C) in ccRCC tissue and ccRCC‐derived cell lines serves as a catalyst for tumor growth by amplifying nuclear factor‐kappa B (NF‐κB) activity. …”
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Autophagy is involved in the toxicity of the biocontrol agent GC16 against Tetranychus pueraricola (Acari: Tetranychidae) based on transcriptomic and proteomic analyses by Yanyan He, Guangzu Du, Guang Wang, Huiming Guan, Shusheng Zhu, Bin Chen, Xiahong He, Youyong Zhu

“…Results Transcriptomic and proteomic analyses revealed 2717 differentially expressed genes (DEGs) and 374 differentially expressed proteins (DEPs) between the GC16-treated and control mites. …”
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Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of β-Adrenergic Receptors to Phosphoinositide 3-Kinase by Julie Simard, Matthieu Boucher, Rachel Massé, Terence E. Hébert, Guy Rousseau

“…An agonist-occupied β2-adrenergic receptor (β2-AR) recruits G protein receptor kinase-2 (GRK2) which is recruited to the membrane. …”
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Ameliorative Effects of Polygonatum kingianum Polysaccharides on Lipid Metabolism Disorders and Brain Function Impairments in Obese Mice by XU Yuncong, YAN Qiaojuan, ZHU Chunhua, YANG Shaoqing, LI Houjiang, JIANG Zhengqiang

“…The results showed that PKP (1 500 mg/kg) reduced body mass gain by 29.6% in obese mice, significantly alleviated dyslipidemia and pathological changes in liver and adipose tissue, and promoted the expression of peroxisome proliferator-activated receptors α (PPARα) in liver (46.0%) and the expression of uncoupling protein 1 in adipose tissue (59.0%). PKP significantly increased autonomic activity and suppressed anxiety-like behavior in obese mice. …”
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A systemic analysis of Creutzfeldt Jakob disease cases in Asia by Urwah Rasheed, Sana Khan, Minahil Khalid, Aneeqa Noor, Saima Zafar

“…Sporadic CJD, which occurs for reasons unbeknown to science (85% of known cases), Genetic or Familial CJD which is characterized by the presence of mutations in the human prion protein (PRNP) gene (10–15% cases) and Iatrogenic CJD that occurs via accidental transmission through medical and surgical procedures (1–2% cases). …”
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Role of Serum and Ultrasonometric Markers in the Early Diagnosis of Malignant Transformation of Periductal Liver Fibrosis in Patients with <i>Opisthorchis Felineus</i> Infection by A. Е. Kovshirina, O. S. Fedorova, Yu. V. Kovshirina, V. A. Petrov

“…A comprehensive one-stage comparative study was conducted in a case-control design, using the assessment of biochemical parameters (lactate, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, glucose, direct and indirect bilirubin, high-density lipoproteins and low-density lipoproteins, triglycerides, urea and total protein), immunological parameters (transforming growth factor beta) and tumor markers (CA 19-9, СYFRA 21-1) in blood serum samples obtained from patients with O. felineus infection, with and without periductal fibrosis, O. felineus-infected patients with cholangiocarcinoma and representatives of control groups. …”
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Odontogenic differentiation of dental pulp stem cells by glycogen synthase kinase-3β inhibitory peptides by Vidhyashree Rajasekar, Mohamed Mahmoud Abdalla, Mohammed S. Basbrain, Prasanna Neelakantan, Cynthia KY Yiu

“…Additionally, L803-mts showed a significant increase (p < 0.05) in the expression of DSPP, DMP-1, Runx-2, along with increased protein expression of DSPP and DMP-1 compared to the control. …”
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Antidiabetic Rosiglitazone Reduces Soluble Intercellular Adhesion Molecule-1 Level in Type 2 Diabetic Patients with Coronary Artery Disease by Guang Wang, Zhe Zhang, Jie Yu, Fuchun Zhang, Liyun He, Jinru Wei, Jieming Mao, Xian Wang

“…In addition, plasma levels of C-reactive protein were significantly reduced from baseline levels. …”
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PSMA antibody, humanized PSMA.CAR10.3, or Cetuximab increases prostate cancer localization of NF-κB p50-deficient immature myeloid cells (p50-IMC) and phagocytosis by their macroph... by Mohammad A. Alzubi, Theresa Barberi, Alan D. Friedman

“…Lack of tolerance of human PSMA or EGFR by immune-competent mice and lack of expression of human PSMA protein in the prostate of AR2-Probasin-hPSMA transgenic mice precluded our ability to determine whether human-specific PSMA or EGFR antibody or PSMA.CAR10.3 increases anti-tumor efficacy of murine p50-IMC. …”
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Effects of Exercise Training on Molecular Markers of Lipogenesis and Lipid Partitioning in Fructose-Induced Liver Fat Accumulation by Siham Yasari, Denis Prud'homme, Frédérique Tesson, Marek Jankowski, Jolanta Gutkowska, Emile Levy, Jean-Marc Lavoie

“…Fructose refeeding was associated with an increase in hepatic lipid content, endocannabinoid receptor 1, sterol regulatory element-binding protein1c, and stearoyl-CoA desaturase1 gene expression in both Sed and TR rats. …”
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Quality Evaluation of Herbal Yoghurt Produced Using Cinnamon (Cinnamon cassia), Garlic (Allium sativum) and Ginger (Zingiber officinale) by Ifeoma Elizabeth Mbaeyi-Nwaoha, Augustina Chinechendu Uzoigwe, Helen Onyeaka

“…The proximate analysis revealed variations in moisture (86.67% to 87.79%), protein (4.26% to 4.28%), fat (3.82% to 3.91%), ash (0.88% to 0.95%) and carbohydrates (0.12% to 1.73%). …”
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