T4 Phage Displaying Dual Antigen Clusters Against H3N2 Influenza Virus Infection by Shenglong Liu, Mengzhou Lin, Xin Zhou

“…Specifically, we fused the Soc protein with the HA1 antigen and the Hoc protein with the M2e antigen, assembling them onto a T4 phage that lacks Soc and Hoc proteins (Soc⁻Hoc⁻T4), thereby constructing a nanovaccine that concurrently presents both HA1 and M2e antigens. …”
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Molecular docking of ferulic acid, bakuchiol and niazirin on peroxisome proliferator-activated receptor gamma (PPAR-γ) as anti-diabetic agents by Anjar Hermadi Saputro, Riri Fauziyya, Sarmoko, Iwan Syahjoko Saputra

“… Background: Diabetes mellitus remains one of the most dangerous illnesses worldwide. The PPAR-gamma protein plays a crucial role in lipid and carbohydrate metabolism, making it a key target for diabetes therapy. …”
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Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice by E. A. Litvinova, K. M. Achasova, M. A. Borisova, S. V. Zhenilo, E. B. Prokhortchouk, E. N. Kozhevnikova

“…One of such transcription factors is “zinc fnger” domain-containing protein Kaiso which is able to bind to methylated DNA. …”
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Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular Carcinoma by Gorkem Odabas, Metin Cetin, Serdar Turhal, Huseyin Baloglu, A. Emre Sayan, Tamer Yagci

“…Our observations in cancer databases that PLXNC1 is upregulated in HCC led us to investigate the expression profile of Plexin C1 mRNA and protein in HCC cell lines and tissues. Methods. A recombinant protein encompassing part of the extracellular domain of Plexin C1 was used as an antigen for monoclonal antibody development. …”
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A Snake Venom-Secreted Phospholipase A2 Induces Foam Cell Formation Depending on the Activation of Factors Involved in Lipid Homeostasis by Elbio Leiguez, Karina Cristina Giannotti, Mariana do Nascimento Viana, Márcio Hideki Matsubara, Cristina Maria Fernandes, José Maria Gutiérrez, Bruno Lomonte, Catarina Teixeira

“…Moreover, PPAR-β/δ, but not PPAR-γ, is involved in MT-III-induced PLIN2 protein expression, and both PPAR-β/δ and PPAR-γ upregulated CD36 protein expression, which contributes to MT-III-induced COX-2 expression. …”
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Translational regulation of PKD1 by evolutionarily conserved upstream open reading frames by Lei Chen, Xia Gao, Xiangshen Liu, Ye Zhu, Dong Wang

“…Antisense oligos targeting the uORFs reduce luciferase protein levels without altering luciferase mRNA levels. …”
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InceptionDTA: Predicting drug-target binding affinity with biological context features and inception networks by Mahmood Kalemati, Mojtaba Zamani Emani, Somayyeh Koohi

“…In this paper, we introduce InceptionDTA, a novel drug-target binding affinity prediction model that leverages CharVec, an enhanced variant of Prot2Vec, to incorporate both biological context and categorical features into protein sequence encoding. InceptionDTA utilizes a multi-scale convolutional architecture based on the Inception network to capture features at various spatial resolutions, enabling the extraction of both local and global features from protein sequences and drug SMILES. …”
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Circulating Cytokines and Growth Factors in Pediatric Pulmonary Hypertension by Mark Duncan, Brandie D. Wagner, Keri Murray, Jenna Allen, Kelley Colvin, Frank J. Accurso, D. Dunbar Ivy

“…We have assessed a panel of circulating proteins in children with PH to investigate their value as predictive and/or prognostic biomarkers. …”
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Mechanism of Radix Bupleuri and Hedysarum Multijugum Maxim drug pairs on liver fibrosis based on network pharmacology, bioinformatics and molecular dynamics simulation. by Lefei Yu, Pan Yu, Yongchang Cao, Weiya Cao

“…Totally, 155 intersection targets between RB-HMM and liver fibrosis were identified. In the protein-protein interaction (PPI) network, the top 10 hub targets with the highest node connection values were TNF, IL-6, AKT1, EGFR, HIF1A, PPARG, CASP3, SRC, MMP9 and HSP90AA1. …”
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Comprehensive analysis of 111 Pleuronectiformes mitochondrial genomes: insights into structure, conservation, variation and evolution by Suxu Tan, Wenwen Wang, Jinjiang Li, Zhenxia Sha

“…Results Our analysis revealed a conserved gene content of protein-coding genes and rRNA genes, but varying numbers of tRNA genes and control regions across species. …”
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Computational and In silico study of novel fungicides against combating root rot, gray mold, fusarium wilt, and cereal rust. by Mollah Naimuzzaman, Md Mahabub Hasan, Ajoy Kumer, Abu Yousuf Hossin, Mohammad Harun-Ur-Rashid, Swapan Kumar Roy, Abu Noman Faruq Ahmmed, Jamal Uddin

“…The exploration of potential candidates for fungicides against four fungal proteins that cause some vital plant diseases, namely Phytophthora capsici, Botrytis cinerea, Fusarium oxysporum f. sp. lycopersici, and Puccinia graminis f. sp. tritici, was conducted using in silico, molecular docking simulations, and molecular dynamic (MD) simulation for selecting the nature of binding affinity with actives sites of proteins. …”
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Glycogen Synthase Kinase-3 Regulates Production of Amyloid-β Peptides and Tau Phosphorylation in Diabetic Rat Brain by Zhong-Sen Qu, Liang Li, Xiao-Jiang Sun, Yu-Wu Zhao, Jin Zhang, Zhi Geng, Jian-Liang Fu, Qing-Guo Ren

“…Here we tried to investigate the production of amyloid-β peptides (Aβ) and phosphorylation of microtubule-associated protein tau in DM rats and elucidate the role of GSK-3 and Akt (protein kinase B, PKB) in these processes. …”
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Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen by Ling Zhang, Xiaocui Yang, Kaijun Xiao, Yuyi Lu, Chunhai Li, Zijin Zhang

“…The results showed that the molecular weight of the purified enzyme protein was 31.0 kDa. The amino acid sequence with a consistency of 64.04% and one three-dimensional structure simulation diagram of the purified enzyme protein were obtained by LC-MS-MS, which suggested that the protein might belong to subtilisin. …”
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Influence of Inflammatory Cytokines IL-1β and IFNγ on Sarcoplasmic Aggregation of p62 and TDP-43 in Myotubes by Bryony McCord, Richard M. Day

“…Non-inflammatory features are also observed, like the sarcoplasmic accumulation of proteins including TDP-43 and p62. This study aimed to investigate the effect of IFNγ and interleukin 1-β (IL-1β) on TDP-43 and p62 aggregation in vitro. …”
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Effect of Rho-Kinase and Autophagy on Remote Ischemic Conditioning-Induced Cardioprotection in Rat Myocardial Ischemia/Reperfusion Injury Model by Jie Gao, Feng Min, Shasha Wang, Heng Lv, Huan Liang, Ben Cai, Xianjie Jia, Qin Gao, Ying Yu

“…Histopathology and ultrastructure and myocardial enzymes’ expression were evaluated to determine the degree of cardiac injury. The protein expression of the Rho-kinase substrates myosin light chain (MLC) and myosin phosphatase target subunit 1 (MYPT1), autophagy-related protein light chain 3-II (LC3-II) and Beclin 1, and protein kinase B (AKT) was measured in the myocardial tissue. …”
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Downregulation of Thromboxane A2 Receptor Occurs Mainly via Nuclear Factor-KappaB Signaling Pathway in Rat Renal Artery by Yaping Zhang, Man Mi, Yan-Hua Xie, Si-Wang Wang, Lars Edvinsson, Cang-Bao Xu

“…This decreased contractile response to U46619 was paralleled with decreased TP receptor mRNA and protein expressions in the renal artery smooth muscle cells. …”
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Comparison of Two Soy Globulins on the Dynamic-Mechanical Properties of the Dough and the Quality of Steamed Bread by Xue-Li Gao, Fu-Sheng Chen, Li-Fen Zhang, Guan-Hao Bu, Ming-Tao Fan

“…To investigate the effect of the soy protein concentrate (CSP) and 7S and 11S soy globulin on wheat dough and steamed bread (SB), mixing properties of the dough were assessed by farinograph and dynamic-mechanical analyzer (DMA). …”
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Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…Finally, after taking interaction genes between DEGs and DMGs, abnormal methylation-modified genes were identified, followed by protein–protein interaction analysis and expression validation using reverse transcriptase polymerase chain reaction. …”
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Electroacupuncture-Regulated miR-34a-3p/PDCD6 Axis Promotes Post-Spinal Cord Injury Recovery in Both In Vitro and In Vivo Settings by Lili Ma, Lizhong Ma, Yu Yang, Ting Chen, Limin Wang, Qilong Deng

“…Our in vitro results showed that miR-34a-3p might promote the in vitro differentiation of neural stem cell (NSC) through suppressing PDCD6 and regulating other important neural markers such as fibroblast growth factor receptor 1 (FGFR1), MAP1/2 (MAP kinase kinases 1/2), myelin basic protein (MBP), βIII-tubulin Class III β-tubulin (βIII tubulin), and glial fibrillary acidic protein (GFAP). …”
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Second-Generation Triple Reporter for Bioluminescence, Micro–Positron Emission Tomography, and Fluorescence Imaging by Aparna H. Kesarwala, Julie L. Prior, Jinwu Sun, Scott E. Harpstrite, Vijay Sharma, David Piwnica-Worms

“…Furthermore, a triple-reporter construct was developed in which monster green fluorescent protein (MGFP), a recently available enhanced fluorescent protein, was introduced into the fusion vector downstream of an internal ribosome entry site (IRES) to allow analysis by fluorescence microscopy or flow cytometry without compromising the specific activities of the upstream fusion components. …”
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