Delivery of FGF18 using mRNA-LNP protects the cartilage against degeneration via alleviating chondrocyte senescence by Keyu Kong, Baixing Li, Yongyun Chang, Chen Zhao, Hua Qiao, Minghao Jin, Xinru Wu, Wenxuan Fan, Liao Wang, Yansong Qi, Yongsheng Xu, Zanjing Zhai, Peixiang Ma, Huiwu Li

“…Abstract Background Osteoarthritis (OA) is a degenerative joint disease with an immense unmet medical need. FGF18 protein is a potential regenerative factor for cartilage repair. …”
Get full text

Proteomic analysis of spinal dorsal horn in prior exercise protection against neuropathic pain by Binglin Chen, Jiabao Guo, Chan Gong, Chenchen Zhu, Yang Wu, Shengbo Wang, Yili Zheng, Haixia Lu

“…Gene Ontology (GO) annotations, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and protein–protein interaction networks were employed to explore the biological importance of DEPs. …”
Get full text

Nutritional value and chemical composition of quinoa leaves (Chenopodium quinoa Wild.) at different phenological stages by Edwin O. Baldeón, Augusto Sánchez-Pizarro, Ritva Repo-Carrasco-Valencia, José M. Barat, Raúl Grau, Attilio Israel Cadenillas-Martínez, Raúl Comettant-Rabanal

“…Salcedo reached this protein content at the second phenological stage, with 38.25 g/100g. …”
Get full text

NGHIÊN CỨU KHẢ NĂNG ỨC CHẾ VI KHUẨN GÂY BỆNH VIÊM ĐƯỜNG HÔ HẤP VÀ VÒM HỌNG CỦA CÁC HOẠT CHẤT TÁCH CHIẾT TỪ CÂY KINH GIỚI (Elsholatzia cillata) BẰNG THỰC NGHIỆM VÀ MÔ PHỎNG... by Vĩnh Phú Nguyễn, Hoàng Bách Võ, Thị Phương Nga Hoàng, Quang Huy Trần, Đại Châu Nguyễn, Thị Ái Nhung Nguyễn, Tứ Quý Phan, Thị Thanh Hải Nguyễn

“…Nghiên cứu khả năng ức chế vi khuẩn gây bệnh viêm đường hô hấp (Streptococcus pneumoniae với protein 5XU1) và vi khuẩn vòm họng (Strepcococcus pyogene với protein 4XCH) của các hợp chất chất trong điều kiện phòng thí nghiệm (in vitro) và mô phỏng docking phân tử (in silico). …”
Get full text

Chemical cross‐linking facilitates antigen uptake and presentation and provides improved protection from Mpox with a dual‐antigen subunit vaccine by Long Chen, Chao Shang, Zihao Wang, Mengzhu Zheng, Cuiling Zhang, Dapeng Li, Zhanqun Yang, Yuchao Dong, Yuru Xu, Yunsheng Yuan, Shiyong Fan, Wu Zhong, Jian Lin, Xiao Li

“…Abstract Antigen uptake, processing, and presentation are crucial for the immune responses of protein‐based vaccines. Herein, we introduced a reversible chemical cross‐linking strategy to engineer protein antigens, which can be tracelessly removed upon antigen‐presenting cell (APC) uptake and cellular reduction. …”
Get full text

Ursodeoxycholic acid protects against sepsis-induced acute kidney injury by activating Nrf2/HO-1 and inhibiting NF-κB pathway by Yunpeng Lou, Hongguang Shi, Ning Sha, Feifei Li, Xiaofeng Gu, Huiyan Lin

“…Kidney injury was evaluated by HE staining and biochemical markers, including serum creatinine (Cr), blood urea nitrogen (BUN), urinary protein, neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), N-acetyl-β-D-glucosaminidase (NAG), and retinol binding protein (RBP). …”
Get full text

Cardiovascular Risk Biomarkers in Women with and Without Polycystic Ovary Syndrome by Manjula Nandakumar, Priya Das, Thozhukat Sathyapalan, Alexandra E. Butler, Stephen L. Atkin

“…Functional enrichment and protein–protein interaction analyses elucidated the pathways involved. …”
Get full text

Growth performance of African catfish Clarias gariepinus cultured in biofloc system at high stocking density by Petrus Hary Tjahja Soedibya, Taufik Budhi Pramono, Emyliana Listiowati

“…During rearing period, fish were given the artificial feed with protein contents of 28.75% with the frequency of twice a day, as much as 3% of the body weight. …”
Get full text

Testicular Nitric Oxide and Thiobarbituric Acid Reactive Substances Levels in Obstructive Azoospermia: A Possible Role in Pathophysiology of Infertility

“…Testicular tissue nitrate/nitrite and TBARS levels were <mml:math alttext="$35.7pm 3.1$"><mml:mrow><mml:mn>35.7</mml:mn><mml:mo>&#x00B1;</mml:mo><mml:mn>3.1</mml:mn></mml:mrow></mml:math><mml:math alttext="$mu$"><mml:mi>&#x03BC;</mml:mi> </mml:math>mol/g protein and <mml:math alttext="$3.7pm 0.1$"><mml:mrow> <mml:mn>3.7</mml:mn><mml:mo>&#x00B1;</mml:mo><mml:mn>0.1</mml:mn> </mml:mrow></mml:math> nmol/g protein in Group-1, and <mml:math alttext="$19.3pm 0.7$"> <mml:mrow><mml:mn>19.3</mml:mn><mml:mo>&#x00B1;</mml:mo><mml:mn>0.7</mml:mn> </mml:mrow></mml:math><mml:math alttext="$mu$"><mml:mi>&#x03BC;</mml:mi> </mml:math>mol/g protein and <mml:math alttext="$3.1pm 0.1$"><mml:mrow><mml:mn>3.1</mml:mn><mml:mo>&#x00B1;</mml:mo><mml:mn>0.1</mml:mn></mml:mrow></mml:math> nmol/g protein in Group-2, respectively. …”
Get full text

Identification of potential druggable targets for endometriosis through Mendelian randomization analysis by Peng Chen, Xin Wei, Xiao-Ke Li, Yi-Hang Zhou, Yi-Hang Zhou, Qi-Fang Liu, Ling Ou-Yang

“…This study aimed to identify both cerebrospinal fluid (CSF) and plasma protein markers as promising therapeutic targets for EM.MethodsWe utilized Mendelian randomization (MR) analysis to explore potential disease-causing proteins, utilizing genetic datasets from genome-wide association studies (GWAS) and protein quantitative trait loci (pQTL) analyses. …”
Get full text

Induced Inflammatory and Oxidative Markers in Cerebral Microvasculature by Mentally Depressive Stress by Yuequan Zhu, Yazeed Haddad, Ho Jun Yun, Xiaokun Geng, Yuchuan Ding

“…Cerebral microvasculature was isolated from the CRS and controls (n=10/group). mRNA and protein expressions of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), vascular cell adhesion molecule-1 (VCAM-1), and macrophage chemoattractant protein-1 (MCP-1) in the brain vessels were measured by real-time PCR and Western blot (n=10/group). …”
Get full text

The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…Knockdown of the lncRNA ENSG00000253385.1 could inhibit the proliferation, migration and invasion, promote the apoptosis, and result in increases in the necroptosis-related proteins p-receptor-interacting protein kinase 3 (RIPK3)/RIPK3 and p-mixed lineaae kinase domain-like protein (MLKL)/MLKL and a decrease in the VDAC1 protein levels in ESCC cells, whereas miR-16-2-3p inhibition rescued these effects. …”
Get full text

Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes by Sirima Sangkapat, Rattiporn Boonnop, Jeerawat Pimta, Napason Chabang, Bodee Nutho, Promsuk Jutabha, Sunhapas Soodvilai

“…The present study aims to evaluate the interaction potential of pinocembrin with cytochrome P450 (CYP450: CYP2B6, CYP2C9, and CYP2C19) and drug transporters including organic anion transporters (OAT1 and OAT3), organic cation transporters (OCT1 and OCT2), multidrug and toxin extrusion (MATE1 and MATE2, P-glycoprotein (P-gp), and breast cancer resistance protein (BCRP). <b>Methods</b>: The interactions of pinocembrin on drug transporters were determined in the Madin–Darby canine kidney (MDCK) cells overexpressing human (h)OAT1 or hOAT3 and in the Chinese hamster ovary (CHO-K1) cells overexpressing hOCT1, hOCT2, hMATE1, or hMATE2. …”
Get full text

Metabolism: A Novel Shared Link between Diabetes Mellitus and Alzheimer’s Disease by Yanan Sun, Cao Ma, Hui Sun, Huan Wang, Wei Peng, Zibo Zhou, Hongwei Wang, Chenchen Pi, Yingai Shi, Xu He

“…Emerging evidence suggests that both oxidative stress and mitochondrial dysfunction are related to amyloid-β (Aβ) pathology. Protein changes in the unfolded protein response or endoplasmic reticulum stress can regulate Aβ production and are closely associated with tau protein pathology. …”
Get full text

Dim blue light at night worsens high-fat diet-induced kidney damage via increasing corticosterone levels and modulating the expression of circadian clock genes by Wenji Ren, Zixu Wang, Yulan Dong, Jing Cao, Ting Gao, Qingyun Guo, Yaoxing Chen

“…Additionally, dBL activated the NF-κB signaling pathway and resulted in elevated protein levels of TLR4, p-IκB, p-P65, and TNF-α. Furthermore, dBL increased BAX protein levels and decreased BCL2 protein levels. …”
Get full text

Short-Term Exercise Training Improves Insulin Sensitivity but Does Not Inhibit Inflammatory Pathways in Immune Cells from Insulin-Resistant Subjects by Sara M. Reyna, Puntip Tantiwong, Eugenio Cersosimo, Ralph A. DeFronzo, Apiradee Sriwijitkamol, Nicolas Musi

“…Peripheral mononuclear cells (PMNC) were obtained for determination of Toll-like receptor (TLR) 2 and 4 protein content and mitogen-activated protein kinase phosphorylation. …”
Get full text

Immunofluorescence for Detection of TOR Kinase Activity In Situ in Photosynthetic Organisms by Ana Lando, María De Marco, Andrea Cumino, Giselle Martínez-Noël

“…The target of rapamycin (TOR) is a central hub kinase that promotes growth and development in all eukaryote cells. TOR induces protein synthesis through the phosphorylation of the S6 kinase (S6K), which, in turn, phosphorylates ribosomal S6 protein (RPS6) increasing this anabolic process. …”
Get full text

Changes in RNA Splicing: A New Paradigm of Transcriptional Responses to Probiotic Action in the Mammalian Brain by Xiaojie Yue, Lei Zhu, Zhigang Zhang

“…The Lacidofil^® altogether altered 2941 differential splicing events, predominantly, skipped exon (SE) and mutually exclusive exon (MXE) events. Protein–protein interactions and a KEGG analysis of differentially spliced genes (DSGs) revealed consistent enrichment in the spliceosome and vesicle transport complexes, as well as in pathways related to neurodegenerative diseases, synaptic function and plasticity, and substance addiction across brain regions. …”
Get full text

E6AP is essential for the proliferation of HPV-positive cancer cells by preventing senescence. by Alicia Avenhaus, Milica Velimirović, Julia Bulkescher, Martin Scheffner, Felix Hoppe-Seyler, Karin Hoppe-Seyler

“…The formation of a trimeric complex between the HPV E6 oncoprotein, the cellular ubiquitin ligase E6AP and the p53 tumor suppressor protein leads to proteolytic p53 degradation and plays a central role for HPV-induced cell transformation. …”
Get full text

Effect of <i>Chlorella</i> on hematological parameters and nutrient bioavailability in the diet of rhesus monkeys (<i>Macaca mulatta</i>) by N. V. Gaponov, Al. V. Panchenko, An. V. Panchenko, Yu. P. Chuguev

“…Thus, the digestibility of crude protein in the animals receiving algae suspension increased by 4.18% (p &lt; 0.05), that of crude fat – by 4.70% (p &lt; 0.01), crude fiber – by 4.14% (p &lt; 0.05) and crude ash – by 12.32% (p &lt; 0.001). …”
Get full text