Synthesis, Characterization, and In Vivo Cytokinome Profile of IL-12-Loaded PLGA Nanospheres by Ryan A. Lacinski, Justin E. Markel, Jabeen Noore, Hillary G. Pratt, Brock A. Lindsey

“…Disparate zeta (ζ) potentials obtained from both protein-loaded and protein-unloaded batches suggested surface adsorption of protein, and confocal microscopy of BSA-FITC-loaded nanospheres confirmed the presence of protein within the polymeric shell. …”
Get full text

The immune-related gene CD5 is a prognostic biomarker associated with the tumor microenvironment of breast cancer by Yi Zhao, Hengheng Zhang, Wenwen Wang, Guoshuang Shen, Miaozhou Wang, Zhen Liu, Jiuda Zhao, Jinming Li

“…Univariate Cox regression analysis of overall survival was performed on the DEGs, and a protein–protein interaction network of their protein products was generated. …”
Get full text

Effects of dietary Bacillus NP5 and sweet potato extract on growth and digestive enzyme activity of dumbo catfish Clarias sp. by Achmad Noerkhaerin Putra, Yayu Romdhonah

“…The addition of synbiotic to diets showed the best result on final weight, specific growth rate, feed conversion ratio, protein retention, protein efficiency ratio, and protease activities among other treatments.   …”
Get full text

Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion by Umme Salma, Min Xue, Md Sayed Ali Sheikh, Xiaoming Guan, Bin Xu, Aiqian Zhang, Lihua Huang, Dabao Xu

“…We found that the plasma concentrations and protein expressions of TGF-β1 were significantly increased in patients and experimental rabbits compared to those in controls (P<0.05). …”
Get full text

Interleukin-17 prevents oxidative stress from damaging osteoblast formation by inhibiting autophagic degradation of metallothionein-2 by Xueyan Ling, Cuixia Wang, Qin Feng, Tao Zhang

“…Moreover, in the presence of H2O2, IL-17A inhibited autophagic activity and promoted MT-2 protein expression in osteoblast precursors. Importantly, IL-17A-promoted MT-2 protein levels and -inhibited ROS production were reversed by autophagy activation with rapamycin. …”
Get full text

Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats by Lin Zhang, Hua Peng, Jin Xu, Yixin Xu, You Yin, Bin He, Jianhua Zhuang

“…At weeks 1, 2, 3, and 4 after modeling, there were no significant differences between the control group and the DOPS group in the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 (all P>0.05); the mRNA and protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, and p38 were significantly increased, while the phosphorylation of MKP-1 was decreased in the model group compared with the control group. …”
Get full text

Investigation of Scutellaria Barbata’s immunological mechanism against thyroid cancer using network pharmacology and experimental validation by Gen Ouyang, Yuangui Zhu, Zhehao Ouyang

“…TC targets were then filtered, and common targets were selected to build a protein-protein interaction network. GO and KEGG enrichment analyses were performed. …”
Get full text

Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink by Lejla Gul, Anna Julia Elias, Tanvi Tambaku, Marton Olbei, Emily Watters, Balazs Bohar, Dezso Modos, Matthew Madgwick, Tamas Korcsmaros

“…Here, we present a protocol for predicting host-microbe protein-protein interactions and their downstream effects using MicrobioLink. …”
Get full text

Effect of Brewery Spent Grain Level and Fermentation Time on the Quality of Bread by Tadlo Yitayew, Demewez Moges, Neela Satheesh

“…BSG (brewery spent grain) is the most frequent by-product from the beer industry, which is high in protein, fiber, and minerals. This research was carried out to improve the nutritional content of bread by adding BSG to wheat flour. …”
Get full text

The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages by Hwa-Jin Chung, Wonil Koh, Won Kyung Kim, Joon-Shik Shin, Jinho Lee, Sang Kook Lee, In-Hyuk Ha

“…Shinbaro3 also downregulated the mRNA and protein expression of inflammatory mediators in a dose-dependent manner. …”
Get full text

Electroacupuncture Mitigates Hippocampal Cognitive Impairments by Reducing BACE1 Deposition and Activating PKA in APP/PS1 Double Transgenic Mice by Yinshan Tang, Shujun Shao, Yu Guo, You Zhou, Jin Cao, Anping Xu, Jihong Wu, Zhigang Li, Dulian Xiang

“…Increased amyloid-β (Aβ) plaque deposition is thought to be the main cause of Alzheimer’s disease (AD). β-Site amyloid precursor protein cleaving enzyme 1 (BACE1) is the key protein involved in Aβ peptide generation. …”
Get full text

The AMPK–mTOR Pathway Is Inhibited by Chaihu Shugan Powder, Which Relieves Nonalcoholic Steatohepatitis by Suppressing Autophagic Ferroptosis by Zheng Liang, Dajin Pi, Jianwei Zhen, Haizhen Yan, Chuiyang Zheng, July Liang Chen, Wen Fan, Qingliang Song, Jinyue Pan, Dongdong Liu, Maoxing Pan, Qinhe Yang, Yupei Zhang

“…In addition, a total of 1009 genes exhibited enrichment in both the autophagy and ferroptosis pathways. The liver protein levels of Adenosine monophosphate-activated protein kinase–mammalian target of rapamycin (AMPK–mTOR)-mediated autophagy and ferroptosis markers, such as p-AMPKα/AMPKα, p-mTOR/mTOR, Beclin-1, microtubule associated protein 1 light chain 3 gamma (LC3), p62 (sequestosome 1 [SQSTM1/p62]), Kelch-like ECH-associated protein 1 (KEAP1), nuclear factor erythroid 2-related factor 2 (Nrf-2), ferritin heavy chain 1 (FTH1), and glutathione peroxidase 4 (GPX4), were restored by CSP. …”
Get full text

SPARC Levels Modulate the Capacity of Mitomycin to Inhibit the Proliferation of Human Tenon’s Capsule Fibroblasts by Yuanyuan Guo, Shouxiang Ni, Weiyan Zhou, Jiangping Hou, Jiaquan Shen

“…MMC, si-SPARC, or SPARC protein was used when needed to evaluate the cell densities under different conditions, by which the role of SPARC in MMC-mediated antifibrotic process was identified. …”
Get full text

PPARs: Nuclear Receptors Controlled by, and Controlling, Nutrient Handling through Nuclear and Cytosolic Signaling by Maria Moreno, Assunta Lombardi, Elena Silvestri, Rosalba Senese, Federica Cioffi, Fernando Goglia, Antonia Lanni, Pieter de Lange

“…We further discuss the roles played by different kinase pathways, including the extracellular signal-regulated kinases/mitogen-activated protein kinase (ERK MAPK), AMP-activated protein kinase (AMPK), Akt/protein kinase B (Akt/PKB), and the NAD+-regulated protein deacetylase SIRT1, serving to control the activity of the PPARs themselves as well as that of a key nutrient-related PPAR coactivator, PPARγ coactivator-1α (PGC-1α). …”
Get full text

Six Novel ATM Gene Variants in Sri Lankan Patients with Ataxia Telangiectasia by D. Hettiarachchi, Hetalkumar Panchal, B. A. P. S. Pathirana, P. D. Rathnayaka, A. Padeniya, P. S. Lai, V. H. W. Dissanayake

“…ATM is a relatively large protein with a molecular weight of 350 kDa and 3,056 amino acids. …”
Get full text

Antipsychotic Drugs Reverse MK801-Inhibited Cell Migration and F-actin Condensation by Modulating the Rho Signaling Pathway in B35 Cells by Yi-Chyan Chen, Fu-Ming Tsai, Mao-Liang Chen

“…MK801-induced psychotic symptoms and also the Ras homolog family member A (RhoA) expression and cell division control protein 42 (cdc42) mRNA modulation in the rat brain have been investigated. …”
Get full text

Dietary Phosphorus Requirement of Giant Gouramy (Osphronemus gouramy Lac.) by M. Agus Suprayudi, Mia Setiawati

“…Pakan yang digunakan dalam penelitian ini adalah pakan semimumi dengan kadar protein 41,0% dan rasio energi- protein 10 kkal/g protein. …”
Get full text

Upregulated GSDMB in Clear Cell Renal Cell Carcinoma Is Associated with Immune Infiltrates and Poor Prognosis by Yuanshan Cui, Zhongbao Zhou, Yumeng Chai, Yong Zhang

“…Relationships between GSDMB expression, clinicopathological variables, and overall survival (OS) were evaluated with multivariate methods as well as Kaplan-Meier survival curves. Protein-protein interaction (PPI) networks were created with STRING. …”
Get full text

The effect of thioredoxin and prochymosin coexpression on the refolding of recombinant alpaca chymosin by S. V. Belenkaya, D. N. Shcherbakov, A. I. Chapoval, T. I. Esina, V. V. Elchaninov

“…However, the chimeric protein accumulates in inclusion bodies in an insoluble form. …”
Get full text

Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice by Chian-Jiun Liou, You-Rong Lai, Ya-Ling Chen, Yi-Hsien Chang, Zih-Ying Li, Wen-Chung Huang

“…In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. …”
Get full text