Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice by Zitao Guo, Dalong Yi, Bo Hu, Lingyu Zhu, Ji Zhang, Yuliang Yang, Chunyu Liu, Yi Shi, Zhenghua Gu, Yu Xin, Huaigao Liu, Liang Zhang

“…In this study, yak bone collagen hydrolysate (YBCH) was produced by mixed proteases and provided to standard-diet mice at a different dose (low dose (LD), medium dose (MD), and high dose (HD)) to investigate its effects on the composition of gut microbiota and short-chain fatty acids (SCFA) production. …”
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Cardiovascular Risk in Systemic Autoimmune Diseases: Epigenetic Mechanisms of Immune Regulatory Functions by Chary López-Pedrera, Carlos Pérez-Sánchez, Manuel Ramos-Casals, Monica Santos-Gonzalez, Antonio Rodriguez-Ariza, Ma José Cuadrado

“…Traditional risk factors, as well as systemic inflammation mediators, including cytokines, chemokines, proteases, autoantibodies, adhesion receptors, and others, have been implicated in the development of these vascular pathologies. …”
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Mealworm-Derived Protein Hydrolysates Enhance Adipogenic Differentiation via Mitotic Clonal Expansion in 3T3-L1 Cells by Hee-Jeong Ryu, Syng-Ook Lee

“…Protein hydrolysates (<1 kDa) were prepared using enzymatic hydrolysis with three proteases (alcalase, flavourzyme, and neutrase) and evaluated for their adipogenic activity in 3T3-L1 preadipocytes. …”
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Effects of Fungi-Mediated Solid-State Fermentation on Phenolic Contents and Antioxidant Activity of Brown and White Teff (Eragrostis tef (Zucc.) Trotter) Grains by Yoseph Asmelash Gebru, Desta Berhe Sbhatu

“…Such changes are suggested to be attributed to activities of fungal enzymes such as amylases, xylanases, and proteases that induce structural breakdown of grain components including cell walls leading to the liberation or synthesis of a variety of phenolic compounds. …”
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Synergistic effect of blocking cancer cell invasionrevealed by computer simulations by Kazuhisa Ichikawa

“…However, the role of MMP-2 and the effect of inhibitors for these ECM-degrading proteases were still obscure. Here we investigated these two problems by using the same model as in the previous report. …”
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Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes by Maria Mesuraca, Olimpio Galasso, Leonardo Guido, Emanuela Chiarella, Stefania Scicchitano, Renaud Vatrinet, Giovanni Morrone, Heather M. Bond, Giorgio Gasparini

“…Articular chondrocytes are responsible for the maintenance of healthy articulations; indeed, dysregulation of their functions, including the production of matrix proteins and matrix-remodeling proteases, may result in fraying of the tissue and development of osteoarthritis (OA). …”
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Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis by M. Isabel García-Laorden, Ingrid Stroo, Sanne Terpstra, Sandrine Florquin, Jan Paul Medema, Cornelis van´t Veer, Alex F. de Vos, Tom van der Poll

“…Granzymes (gzms) are serine proteases traditionally implicated in cytotoxicity and, more recently, in the inflammatory response. …”
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Anti-biofilm and anti-virulence potential of cell free supernatant of Akkermansia muciniphila against Salmonella by Jiaxiu Liu, Wenxiu Zhu, Huanhuan Liu, Xiaomeng Ren, Ningbo Qin, Xiaodong Xia

“…CFS maintained antimicrobial properties after treatment with high temperatures and various proteases, while it lost its antimicrobial activity after pH neutralization. …”
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Tumor-specific cytosol-penetrating antibodies for antigen- and TME-dependent intracellular cargo delivery by Carolin Sophie Dombrowsky, Felix Klaus Geyer, Diana Zakharchuk, Harald Kolmar

“…In addition, presence of the masking moiety to prevent non-specific surface binding, as well as the activation of cytosol-penetrating capabilities in the tumor microenvironment upon release by tumor-specific proteases was confirmed using the catalytic domain of Pseudomonas exotoxin as model cargo for cytosol delivery. …”
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Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals by Ali Zahedi Amiri, Choudhary Ahmed, Subha Dahal, Filomena Grosso, Haomin Leng, Peter Stoilov, Maria Mangos, Johanne Toutant, Lulzim Shkreta, Liliana Attisano, Benoit Chabot, Martha Brown, Mario Huesca, Alan Cochrane

“…Interestingly, while resistant viruses were rapidly isolated for compounds targeting either virus-encoded proteases or polymerases, we have not yet isolated 5342191-resistant variants of coronavirus or influenza. …”
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Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics by Isabelle Boulogne, Charlotte Toustou, Muriel Bardor

“…Therefore, the expression of genes involved in the N-glycan biosynthesis, protein export and secretion, protein quality control and proteasome, as well as those encoding proteases were analyzed and compared. The results pave the way towards optimizing Biologics production in P. tricornutum and highlight that the Pt4, Pt3 Ov and Pt8 strains seem to be the most promising P. tricornutum strains.…”
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Oxidative stress-driven enhanced iron production and scavenging through Ferroportin reorientation worsens anemia in antimony-resistant Leishmania donovani infection. by Souradeepa Ghosh, Krishna Vamshi Chigicherla, Shirin Dasgupta, Yasuyuki Goto, Budhaditya Mukherjee

“…Higher iron utilization by LD-R leads to subsequent iron insufficiency, compensated by increased erythrophagocytosis through the breakdown of SIRPα-CD47 surveillance, orchestrated by a complex interplay of two proteases, Furin and ADAM10. Understanding these mechanisms is crucial for managing LD-R-infections and their associated complications like severe anemia, and may also provide valuable mechanistic insights into understanding drug unresponsiveness developed in other intracellular pathogens that rely on host iron.…”
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Resin-Dentin Bonding Interface: Mechanisms of Degradation and Strategies for Stabilization of the Hybrid Layer by D. E. Betancourt, P. A. Baldion, J. E. Castellanos

“…The enzymatic degradation of collagen matrix, together with resin leaching, has led to seeking strategies that inhibit the endogenous proteases, cross-linking the denudated collagen fibrils and improving the adhesive penetration removing water from the interface. …”
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Immunoinformatic approach to the design of a novel multi-epitope vaccine against Leishmania major fused to human IgG-Fc by Mahmood Fadaie, Zabihollah Shahmoradi, Hossein Khanahmad

“…Background and purpose: Cutaneous leishmaniasis poses significant health and socioeconomic challenges, making vaccine development a top priority, especially in endemic regions. Cysteine proteases, KMP-11, and HASPB proteins are promising candidates for leishmaniasis vaccine development owing to their immunogenic properties and capacity to provoke robust immune responses, as evidenced by different investigations. …”
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Pathogenic Process-Associated Transcriptome Analysis of Stemphylium lycopersici from Tomato by Dezhen Zhang, Wenjuan Chi, Cuicui Wang, Huijie Dai, Jintang Li, Chunlei Li, Fajun Li

“…Additionally, some genes involved in signal regulation or encoding hemicellulose- and cellulose-degrading enzymes and extracellular proteases were commonly upregulated during pathogenesis. …”
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Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study by Bruno Märkl, Jochen Hardt, Simon Franz, Tina Schaller, Gerhard Schenkirsch, Bernadette Kriening, Reinhard Hoffmann, Stefan Rüth

“…The prognostic role of the proteases uPA and PAI-1, as well as tumor budding, in colon cancer, has been investigated previously. …”
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Schistosome feeding and regurgitation. by Patrick J Skelly, Akram A Da'dara, Xiao-Hong Li, William Castro-Borges, R Alan Wilson

“…Proteomics and transcriptomics have revealed, in addition to gut proteases, an amino acid transporter in gut tissue and other hydrolases, ion, and lipid transporters in the lumen, implicating the gut as the site for acquisition of essential lipids and inorganic ions. …”
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Advances in antiviral strategies targeting mosquito-borne viruses: cellular, viral, and immune-related approaches by Ayyaz Khan, Zakirullah, Shahid Wahab, Seong-Tshool Hong

“…It also highlights recent advances in antiviral strategies targeting host factors such as lipid metabolism, ion channels, and proteasomes, as well as viral targets like NS2B-NS3 proteases and nonstructural proteins. Additionally, it explores immunomodulatory therapies to enhance antiviral responses and emphasizes the potential of drug repurposing, bioinformatics, artificial intelligence, and deep learning in identifying novel antiviral candidates. …”
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An Assay System for Plate-based Detection of Endogenous Peptide:N-glycanase/NGLY1 Activity Using A Fluorescence-based Probe by Hiroto Hirayama, Tadashi Suzuki

“…Despite the development of non-radioisotope-based assay systems such as those using S-alkylated RNase or fluorescent-labeled glycopeptides as substrates, these methods are incompatible with crude enzyme sources, primarily due to the degradation of reaction products by contaminating endogenous proteases. We previously developed an assay system using a 5-carboxyfluorescein-labeled glycosylated cyclo-heptapeptide (5FAM-GCP), a substrate remarkably resistant to endogenous peptidase activity. …”
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Characterization of the procoagulant phenotype of amniotic fluid across gestation in rhesus macaques and humans by Chih Jen Yang, Lyndsey E. Shorey-Kendrick, Cristina Puy, Ashley E. Benson, Phillip A. Wilmarth, Ashok P. Reddy, Keith D. Zientek, Kilsun Kim, Adam Crosland, Chaevien S. Clendinen, Lisa M. Bramer, Olivia L. Hagen, Helen H. Vu, Joseph E. Aslan, Owen J.T. McCarty, Joseph J. Shatzel, Brian P. Scottoline, Jamie O. Lo

“…Conclusion: Overall, our findings reveal that the addition of AF to plasma enhances coagulation in a manner dependent on phospholipids as well as the presence of proteases and other proteins that directly regulate coagulation. …”
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