NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice by Jiamin Zhao, Shuangyi Zhang, Zhiguo Gong, Wei Mao, Wenhui Bao, Qianru Li, Yunjie Bai, Feifei Gao, Shuang Feng

“…Deficiencies in apoptosis were evident through altered expression of cysteine-aspartic acid protease 3 (Caspase-3), P53, and B-cell lymphoma-2 (Bcl-2). …”
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Metagenomic insights into the development of microbial communities of straw and leaf composts by Anastasiia K. Kimeklis, Anastasiia K. Kimeklis, Grigory V. Gladkov, Olga V. Orlova, Tatiana O. Lisina, Alexey M. Afonin, Alexey M. Afonin, Tatiana S. Aksenova, Arina A. Kichko, Alla L. Lapidus, Evgeny V. Abakumov, Evgeny V. Abakumov, Evgeny E. Andronov

“…To study this process, a long-term experiment was conducted on the decomposition of oat straw and leaf litter using soil as a microbial inoculum.MethodsCombined analyses of enzymatic activity and NGS data for 16S rRNA gene amplicon and full metagenome sequencing were applied to study taxonomic, carbohydrate-active enzyme (CAZy), and polysaccharide utilization loci (PULs) composition of microbial communities at different stages of decomposition between substrates.ResultsIn straw degradation, the microbial community demonstrated higher amylase, protease, catalase, and cellulase activities, while peroxidase, invertase, and polyphenol oxidase were more active in leaf litter. …”
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Computational and in vitro evaluation of sumac-derived ©Rutan compounds towards Sars-CoV-2 Mpro inhibition by Muzaffar Kayumov, Parthiban Marimuthu, Parthiban Marimuthu, Jamoliddin Razzokov, Jamoliddin Razzokov, Jamoliddin Razzokov, Nurkhodja Mukhamedov, Nurkhodja Mukhamedov, Akmal Asrorov, Nodir S. Berdiev, Jamolitdin F. Ziyavitdinov, Ansor Yashinov, Ansor Yashinov, Yuliya Oshchepkova, Shavkat Salikhov, Sharafitdin Mirzaakhmedov

“…Here, we evaluated the anti-SARS-CoV-2 main protease (Mpro) efficacy of ©Rutan, which contains five polyphenols (R5, R6, R7, R7’, and R8) extracted from sumac Rhus coriaria L. …”
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Double-targeted liposomes coated with matrix metallopeptidase-2-responsive polypeptide nanogel for chemotherapy and enhanced immunotherapy against cervical cancer by Zhimin Bai, Yibo Yang, Zutong Cui, Wenming Liang, Xin Zhang, Zihan Zhang, Jianming Sun, Zhiwei Liu, Kun Li, Ming Shi, Jian Li

“…This study presents a novel nanogel-based drug delivery system, DOX-4PI@CpG@Lipo@Gel (DPCLG), engineered to respond to Matrix Metallopeptidase-2 (MMP-2)—a protease abundant in the tumor microenvironment (TME). …”
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The potential mechanism of Isodon suzhouensis against COVID-19 via EGFR/TLR4 pathways by Hong Duan, Wei Wang, Shu Li, Han Li, Ghulam Jilany Khan, Yong Ma, Fawang Liu, Kefeng Zhai, Henggui Hu, Zhaojun Wei

“…The results from molecular docking studies revealed that rutin, vitexin, isoquercitrin and quercetin had significant binding ability with 3 chymotrypsin like protease (3CLpro) and angiotensin converting enzyme 2 (ACE2). …”
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Probiotic properties of Bacillus licheniformis HN318 and comparison of the effects of its bacterial cells and cultures on growth, immunity and disease resistance of hybrid grouper... by Zhenjie Cao, Qiaoli Yang, Aie Kang, Guotao Wang, Pengshuo Li, Guiping Qiu, Jinge Wang, Chunsheng Liu, Yun Sun, Yun Sun, Yun Sun

“…Cultures of HN318 displayed higher protease, amylase, and antibacterial activities compared to bacterial cells. …”
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Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) by Manju Kohli, Hina Bansal, Muraleedhar Aski, Gyan P. Mishra, B. R. Shashidhar, Anirban Roy, Soma Gupta, Subodh K. Sinha, Brijesh Kumar Mishra, Nikki Kumari, Atul Kumar, Ranjeet Ranjan Kumar, Ramakrishnan M. Nair, Harsh Kumar Dikshit

“…The key candidate genes include VRADI09G06940 (YMD resistance, TIR-NBS-LRR class, chr. 9), VRADI01G05030 [flavonoid biosynthesis; MYB65 transcription factor (TF); chr. 1], VRADI03G07600 (phenol biosynthesis; GATA TF 16; chr. 3), VRADI04G08470 (ascorbic acid; heat shock protein 70 kDa protein; chr. 4), VRADI04G07510 (FRAP; subtilisin-like protease SBT1.9; chr. 4), and VRADI05G02870 (DPPH; vacuolar protein sorting-associated protein 2; chr. 5). …”
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Comparative efficacy of repurposed drugs lopinavir-ritonavir and darunavir-ritonavir in hospitalised COVID-19 patients: insights from a tertiary centre cohort by Dóra Paróczai, Dóra Paróczai, András Bikov, András Bikov, Andreea Blidaru, Emanuel Bobu, Ana Lascu, Ana Lascu, Cristian Ion Mot, Cristian Ion Mot, Stefan Mihaicuta, Stefan Mihaicuta, Stefan Frent, Stefan Frent

“…BackgroundDrug repurposing has become a widely adopted strategy to minimise research time, costs, and associated risks. Combinations of protease inhibitors such as lopinavir and darunavir with ritonavir have been repurposed as treatments for COVID-19. …”
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smu_1558c-mediated regulation of growth and biofilm formation in Streptococcus mutans by Jing Zhou, Jing Zhou, Qizhao Ma, Qizhao Ma, Jingou Liang, Jingou Liang, Yangyang Pan, Yangyang Pan, Yang Chen, Yang Chen, Shuxing Yu, Shuxing Yu, Yaqi Liu, Yaqi Liu, Qiong Zhang, Qiong Zhang, Yuqing Li, Jing Zou, Jing Zou

“…Notably, glucosyltransferases GtfB and GtfC, key enzymes in biofilm formation, were significantly downregulated in the deletion mutant, while ClpL, a Clp-like ATP-dependent protease involved in protein homeostasis under stress conditions, was highly upregulated. …”
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The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity by Daniel Torrente, Enming J. Su, Alí Francisco Citalán-Madrid, Gerald P. Schielke, Daniel Magaoay, Mark Warnock, Tamara Stevenson, Kris Mann, Flavie Lesept, Nathalie Delétage, Manuel Blanc, Erin H. Norris, Denis Vivien, Daniel A. Lawrence

“…Abstract The thrombolytic protease tissue plasminogen activator (tPA) is expressed in the CNS, where it regulates diverse functions including neuronal plasticity, neuroinflammation, and blood-brain-barrier integrity. …”
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Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Cr... by Xiuneng Wang, Min Wan, Zhen Wang, Haitao Zhang, Si Zhu, Xiufei Cao, Ning Xu, Jichang Zheng, Xianyong Bu, Wei Xu, Kangsen Mai, Qinghui Ai

“…Supplementation of 0.1% tributyrin significantly elevated fish intestinal lipase and protease activities compared to FM and FC diets (P<0.05). …”
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Characterization of a novel covS SNP identified in Australian group A Streptococcus isolates derived from the M1UK lineage by Johanna Richter, Amanda J. Cork, Yvette Ong, Nadia Keller, Andrew J. Hayes, Mark A. Schembri, Amy V. Jennison, Mark R. Davies, Kate Schroder, Mark J. Walker, Stephan Brouwer

“…Conversely, expression of the streptococcal cysteine protease SpeB, which is required during initial stages of colonization and onset of invasive disease, is typically lost in such mutants. …”
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Aspergillus fumigatus extract modulates human eosinophils via NOD2 and oxidative stress by Hisashi Sasaki, Jun Miyata, Yusuke Kawashima, Ryo Konno, Masaki Ishikawa, Yoshinori Hasegawa, Ryuta Onozato, Yo Otsu, Emiko Matsuyama, Keeya Sunata, Katsunori Masaki, Hiroki Kabata, Yoshifumi Kimizuka, Tomoe Abe, Shigeharu Ueki, Koichiro Asano, Akihiko Kawana, Koichi Fukunaga

“…Results: The A. fumigatus extract changed the expression of adhesion molecules (CD62L and CD11b) and CD69 on the surface of eosinophils, without affecting their viability, via nucleotide-binding oligomerization domain-containing protein 2 (NOD2) but not protease activity. Investigation using kinase inhibitors showed that A. fumigatus extract-induced modulation was partly mediated via p38 mitogen-activated protein kinases. …”
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Production of Arthrospira platensis algae to increase production efficiency and enhance immune system of grass carp, Ctenopharyngodon idella against Aeromonas hydrophila by Hossein Adineh

“…Results: The growth of fish in the treatments containing spirulina was significantly higher and the feed conversion ratio was lower compared to the control treatment. Highest protease and lipase activities were observed in S2 and S3 treatments, respectively. …”
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Case report: Atypical young case of MV1 Creutzfeldt-Jakob disease with unusually long survival by Lucie Yeongran Ahn, Mark L. Cohen, Mark L. Cohen, Ignazio Cali, Ignazio Cali, Tia Russell, Jessica Ludwig, Xun Jia, Alberto Bizzi, Lawrence B. Schonberger, Ryan A. Maddox, Rohini Paul, Tania C. Ghazarian, Jaspreet Garcha, Mostafa Hammoudi, Brian Stephen Appleby, Brian Stephen Appleby, Brian Stephen Appleby, Brian Stephen Appleby

“…Absence of pathogenic mutations and methionine/valine heterozygosity at codon 129 of the prion protein gene (PRNP), atypical type 1 protease-resistant PrP that lacks or shows underrepresentation of the diglycosylated PrP isoform by western blot analysis, and no acquired prion disease risk factors resulted in a final diagnosis of atypical sCJD. …”
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Treatment failure and drug resistance among the newly reported HIV-infected patients in Taizhou City from 2020 to 2022 by LI Yan, WU Xuanhe, SHI Lu, LI Guixia, WANG Shanling, WANG Yating, CHEN Tailin, WANG Tingting, XIE Yali, SHEN Weiwei, LIN Haijiang, CHEN Xiaoxiao, HE Na

“…The total drug resistance rate was 55.6% (15/27), of which the drug resistance rates of nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs) were 37.0% (10/27), 51.9% (14/27) and 3.7% (1/27), respectively. …”
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Potential implications of granzyme B in keloids and hypertrophic scars through extracellular matrix remodeling and latent TGF-β activation by Alexandre Aubert, Alexandre Aubert, Alexandre Aubert, Jenna Goeres, Jenna Goeres, Jenna Goeres, Amy Liu, Amy Liu, Amy Liu, Martin Kao, Martin Kao, Martin Kao, Katlyn C. Richardson, Katlyn C. Richardson, Katlyn C. Richardson, Karen Jung, Karen Jung, Karen Jung, Boris Hinz, Boris Hinz, Richard I. Crawford, Richard I. Crawford, David J. Granville, David J. Granville, David J. Granville

“…In this study we investigated Granzyme B (GzmB), a serine protease with established roles in fibrosis and scarring through the cleavage of ECM proteins, as a potential new mediator of TGF-β activation in KS and HS. …”
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Apaf-1 is an evolutionarily conserved DNA sensor that switches the cell fate between apoptosis and inflammation by Jie Ruan, Xuxia Wei, Suizhi Li, Zijian Ye, Linyi Hu, Ru Zhuang, Yange Cao, Shaozhou Wang, Shengpeng Wu, Dezhi Peng, Shangwu Chen, Shaochun Yuan, Anlong Xu

“…Abstract Apoptotic protease activating factor 1 (Apaf-1) was traditionally defined as a scaffold protein in mammalian cells for assembling a caspase activation platform known as the ‘apoptosome’ after its binding to cytochrome c. …”
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Genetic determinants of COVID-19 severity and mortality: ACE1 Alu 287 bp polymorphism and ACE1, ACE2, TMPRSS2 expression in hospitalized patients by João Locke Ferreira de Araújo, Átila Duque Rossi, Jessica Maciel de Almeida, Hugo José Alves, Isabela de Carvalho Leitão, Renata Eliane de Ávila, Anna Carla Pinto Castiñeiras, Jéssica da Silva Oliveira, Rafael Mello Galliez, Marlon Daniel Lima Tonini, Débora Souza Faffe, Shana Priscila Coutinho Barroso Barroso, Gustavo Gomes Resende, Cássia Cristina Alves Gonçalves, Terezinha Marta Pereira Pinto Castiñeiras, Amilcar Tanuri, Mauro Martins Teixeira, Renato Santana Aguiar, Cynthia Chester Cardoso, Renan Pedra de Souza

“…Background The angiotensin-converting enzyme 2 (ACE2) and the transmembrane serine protease 2 (TMPRSS2) are central human molecules in the SARS-CoV-2 virus-host interaction. …”
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Role of calpain system in meat tenderness: A review by Z.F. Bhat, James D. Morton, Susan L. Mason, Alaa El-Din A. Bekhit

“…Calpains are intracellular calcium-dependent cysteine proteases found in most eukaryotes. At least three calpains (μ- and m-calpains and calpain 3) and calpastatin, their specific endogenous inhibitor, are found in muscle. …”
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