Oxidative stress-driven enhanced iron production and scavenging through Ferroportin reorientation worsens anemia in antimony-resistant Leishmania donovani infection. by Souradeepa Ghosh, Krishna Vamshi Chigicherla, Shirin Dasgupta, Yasuyuki Goto, Budhaditya Mukherjee

“…Higher iron utilization by LD-R leads to subsequent iron insufficiency, compensated by increased erythrophagocytosis through the breakdown of SIRPα-CD47 surveillance, orchestrated by a complex interplay of two proteases, Furin and ADAM10. Understanding these mechanisms is crucial for managing LD-R-infections and their associated complications like severe anemia, and may also provide valuable mechanistic insights into understanding drug unresponsiveness developed in other intracellular pathogens that rely on host iron.…”
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Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis by M. Isabel García-Laorden, Ingrid Stroo, Sanne Terpstra, Sandrine Florquin, Jan Paul Medema, Cornelis van´t Veer, Alex F. de Vos, Tom van der Poll

“…Granzymes (gzms) are serine proteases traditionally implicated in cytotoxicity and, more recently, in the inflammatory response. …”
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Tumor-specific cytosol-penetrating antibodies for antigen- and TME-dependent intracellular cargo delivery by Carolin Sophie Dombrowsky, Felix Klaus Geyer, Diana Zakharchuk, Harald Kolmar

“…In addition, presence of the masking moiety to prevent non-specific surface binding, as well as the activation of cytosol-penetrating capabilities in the tumor microenvironment upon release by tumor-specific proteases was confirmed using the catalytic domain of Pseudomonas exotoxin as model cargo for cytosol delivery. …”
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Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals by Ali Zahedi Amiri, Choudhary Ahmed, Subha Dahal, Filomena Grosso, Haomin Leng, Peter Stoilov, Maria Mangos, Johanne Toutant, Lulzim Shkreta, Liliana Attisano, Benoit Chabot, Martha Brown, Mario Huesca, Alan Cochrane

“…Interestingly, while resistant viruses were rapidly isolated for compounds targeting either virus-encoded proteases or polymerases, we have not yet isolated 5342191-resistant variants of coronavirus or influenza. …”
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Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics by Isabelle Boulogne, Charlotte Toustou, Muriel Bardor

“…Therefore, the expression of genes involved in the N-glycan biosynthesis, protein export and secretion, protein quality control and proteasome, as well as those encoding proteases were analyzed and compared. The results pave the way towards optimizing Biologics production in P. tricornutum and highlight that the Pt4, Pt3 Ov and Pt8 strains seem to be the most promising P. tricornutum strains.…”
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Resin-Dentin Bonding Interface: Mechanisms of Degradation and Strategies for Stabilization of the Hybrid Layer by D. E. Betancourt, P. A. Baldion, J. E. Castellanos

“…The enzymatic degradation of collagen matrix, together with resin leaching, has led to seeking strategies that inhibit the endogenous proteases, cross-linking the denudated collagen fibrils and improving the adhesive penetration removing water from the interface. …”
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Pathogenic Process-Associated Transcriptome Analysis of Stemphylium lycopersici from Tomato by Dezhen Zhang, Wenjuan Chi, Cuicui Wang, Huijie Dai, Jintang Li, Chunlei Li, Fajun Li

“…Additionally, some genes involved in signal regulation or encoding hemicellulose- and cellulose-degrading enzymes and extracellular proteases were commonly upregulated during pathogenesis. …”
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Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study by Bruno Märkl, Jochen Hardt, Simon Franz, Tina Schaller, Gerhard Schenkirsch, Bernadette Kriening, Reinhard Hoffmann, Stefan Rüth

“…The prognostic role of the proteases uPA and PAI-1, as well as tumor budding, in colon cancer, has been investigated previously. …”
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Advances in antiviral strategies targeting mosquito-borne viruses: cellular, viral, and immune-related approaches by Ayyaz Khan, Zakirullah, Shahid Wahab, Seong-Tshool Hong

“…It also highlights recent advances in antiviral strategies targeting host factors such as lipid metabolism, ion channels, and proteasomes, as well as viral targets like NS2B-NS3 proteases and nonstructural proteins. Additionally, it explores immunomodulatory therapies to enhance antiviral responses and emphasizes the potential of drug repurposing, bioinformatics, artificial intelligence, and deep learning in identifying novel antiviral candidates. …”
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An Assay System for Plate-based Detection of Endogenous Peptide:N-glycanase/NGLY1 Activity Using A Fluorescence-based Probe by Hiroto Hirayama, Tadashi Suzuki

“…Despite the development of non-radioisotope-based assay systems such as those using S-alkylated RNase or fluorescent-labeled glycopeptides as substrates, these methods are incompatible with crude enzyme sources, primarily due to the degradation of reaction products by contaminating endogenous proteases. We previously developed an assay system using a 5-carboxyfluorescein-labeled glycosylated cyclo-heptapeptide (5FAM-GCP), a substrate remarkably resistant to endogenous peptidase activity. …”
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Characterization of the procoagulant phenotype of amniotic fluid across gestation in rhesus macaques and humans by Chih Jen Yang, Lyndsey E. Shorey-Kendrick, Cristina Puy, Ashley E. Benson, Phillip A. Wilmarth, Ashok P. Reddy, Keith D. Zientek, Kilsun Kim, Adam Crosland, Chaevien S. Clendinen, Lisa M. Bramer, Olivia L. Hagen, Helen H. Vu, Joseph E. Aslan, Owen J.T. McCarty, Joseph J. Shatzel, Brian P. Scottoline, Jamie O. Lo

“…Conclusion: Overall, our findings reveal that the addition of AF to plasma enhances coagulation in a manner dependent on phospholipids as well as the presence of proteases and other proteins that directly regulate coagulation. …”
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The Function of SUMOylation and Its Role in the Development of Cancer Cells under Stress Conditions: A Systematic Review by Qi Zhao, Ying Ma, Zugui Li, Kexin Zhang, Minying Zheng, Shiwu Zhang

“…SUMOylation is a dynamic process finely regulated by SUMOylation enzymes and proteases to maintain a balance between SUMOylation and deSUMOylation. …”
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Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> by Longhao Sun, Shanshan Sun, Tianyang Liu, Xinmin Lei, Ruiqi Liu, Junyi Zhang, Shanshan Dai, Jing Li, Yanqin Ding

“…The strain was capable of growing in media with up to 13% NaCl and producing proteases, siderophores, and the plant hormone IAA. …”
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Automation-aided construction and characterization of Bacillus subtilis PrsA strains for the secretion of amylases by Felix Hamburger, Niels Schlichting, Michael Eichenlaub, Paul Igor Costea, Christopher Sauer, Stefan Jenewein, Johannes Kabisch, Johannes Kabisch

“…Following strain selection and deletion of major extracellular proteases, several hundred individual strains were created and screened using a stepwise and modular automation approach combined with amplicon sequencing. …”
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Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies by Huanan Chang, Ziyu Zheng, Hao Li, Yanqiu Xu, Gengyao Zhen, Yao Zhang, Xidong Ren, Xidong Ren, Xinli Liu, Xinli Liu, Deqiang Zhu, Deqiang Zhu

“…Subsequently, the in vitro activation efficiency of TGase was further improved using co-cultivation techniques with neutral proteases secreted by Bacillus amyloliquefaciens CICC10888, and a TGase activity of 16.91 U/mL was achieved, accounting for a 22.71% increase. …”
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Optimization of Protein Hydrolysate from Earthworms (<i>Perionyx excavatus</i>) of Angiotensin-I-Converting Enzyme Inhibitory Activity by One Variable at a Time by Phong Thanh Bui, Truc Anh Hoang, My Thi Thuy Pham, Linh Le Phuong Tran, Oanh Kim Nguyen

“…This study evaluated the ACE inhibitory activity (ACEIA) of protein hydrolysate from earthworms (Perionyx excavatus) by commercial proteases. The results showed that the protein hydrolysate of earthworms gave the highest ACEIA of 85.38 ± 2.31% (at the protein concentration of 2 mg/mL) and IC50 value of 844.64 μg/mL when the earthworms were hydrolyzed with Flavourzyme® 500 MG under optimized conditions such as earthworm:phosphate buffer ratio of 1:6 (w/v), hydrolysis temperature of 50 °C, pH 7, enzyme:substrate ratio of 600 U/g protein and hydrolysis time of 2 h. …”
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Gene Expression Profiling in Dermatitis Herpetiformis Skin Lesions by M. Dolcino, E. Cozzani, S. Riva, A. Parodi, E. Tinazzi, C. Lunardi, A. Puccetti

“…DEGs also indicate overproduction of matrix proteases (MMP9, ADAM9, and ADAM19) and proteolytic enzymes (CTSG, ELA2, CPA3, TPSB2, and CMA1) that may contribute to epidermal splitting and blister formation. …”
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Usp7 contributes to the tail regeneration of planarians via Islet/Wnt1 axis by Ang Liang, Jinglong Liu, Zhiyuan Zhang, Jing Xiao, Dezeng Liu, Zimei Dong, Guangwen Chen

“…Considerable evidence suggests that ubiquitin plays an important role in maintaining homeostasis and regulating regeneration, but the function of Ubiquitin specific proteases 7 (Usp7) on regeneration in planarians remains elusive. …”
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Computational and In silico study of novel fungicides against combating root rot, gray mold, fusarium wilt, and cereal rust. by Mollah Naimuzzaman, Md Mahabub Hasan, Ajoy Kumer, Abu Yousuf Hossin, Mohammad Harun-Ur-Rashid, Swapan Kumar Roy, Abu Noman Faruq Ahmmed, Jamal Uddin

“…Next, two docking tools, such as AutoDock by PyRx and Molecular Docking by Glide from the Schrödinger suite, were used to convey the docking score, and ligand protein interactions against four main proteases, for instance 7VEM, 8H6Q, 8EBB, and 7XDS having name of pathogens: Phytophthora capsici, Botrytis cinerea, Fusarium oxysporum f. sp. lycopersici, and Puccinia graminis f. sp. tritici, respectively. …”
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The genus Entomophthora: bringing the insect destroyers into the twenty-first century by Carolyn Elya, Henrik H. De Fine Licht

“…We discuss evidence for Entomophthora’s adaptation to growth exclusively inside insects, such as producing wall-less hyphal bodies and a unique set of subtilisin-like proteases to penetrate the insect cuticle. However, we are only starting to understand the functions of unusual molecular and genomic characteristics, such as having large > 1 Gb genomes full of repetitive elements and potential functional diploidy. …”
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