In vitro degradation of non-starch polysaccharide residues in the digesta of broilers offered wheat-soy or maize-soy diets by feed enzymes by E. Kim, N.K. Morgan, A.F. Moss, A. Solbak, L. Li, P. Ader, M. Choct

“…The digesta samples were digested in vitro with one of nine enzymes; GH10, GH11 and GH8 xylanase, arabinofuranosidase, a combination of GH10 xylanase and β-glucanase (XYN+BG), β-mannanase, protease, cellulase cocktail and pectate lyase. When using the gizzard digesta as substrate, GH8 xylanase reduced (P<0.005) both insoluble and soluble NSP levels compared to the control, regardless of diet type. …”
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In silico evaluation of missense SNPs in cancer-associated Cystatin A protein and their potential to disrupt Cathepsin B interaction by Shafaat Hossain, Omar Hamza Bin Manjur, Mst Sharmin Sultana Shimu, Tamanna Sultana, Mustafizur Rahman Naim, Shahariar Siddique, Abdullah Al Mamun, Md Miftaur Rahman, Md Abu Saleh, Md Rakibul Hasan, Tania Rahman

“…Cystatin A (CSTA) functions as a cysteine protease inhibitor by forming tight complexes with the cathepsins. …”
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Comparison of various feed additives on nutrient digestibility, digestive enzyme activity, blood metabolite levels, and gut microbiota composition in japanese quail chicks by Hassan Saleh, Omid Jangjou, Mohammad Taher Mirakzehi, Seyedeh Hamideh Hosseni

“…Quails that were administered fumaric acid displayed the highest levels of amylase and protease activity compared to the other groups (17.75 and 0.84 U/I, respectively). …”
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Dynamical Hybrid System for Optimizing and Controlling Efficacy of Plant-Based Protein in Aquafeeds by Serge Dossou, Mahmoud A. O. Dawood, Amr I. Zaineldin, Ibrahim A. Abouelsaad, Kumbukani Mzengereza, Ronick S. Shadrack, Yukun Zhang, Mohamed El-Sharnouby, Hamada A. Ahmed, Mohammed F. El Basuini

“…Amino acid digestibility reflects protein digestibility in most cases. Interestingly, protease, lipase, and amylase activities were better expressed in RM-Koji, RM-Yeast, and FM over RM, respectively. …”
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Cathepsin-D and outcomes in peripartum cardiomyopathy: Results from IPAC by Vincenzo B. Polsinelli, Karen Hanley-Yanez, Charles F. McTiernan, Kalgi Modi, Jennifer Haythe, Hal Skopicki, Uri Elkayam, Leslie T. Cooper, Jr, James D. Fett, Dennis M. McNamara

“…Background: Cathepsin-D is a protease released during oxidative stress that cleaves prolactin (PRL) generating a 16 kDa fragment that is pro-apoptotic, anti-angiogenic, and has been implicated in the pathogenesis of peripartum cardiomyopathy (PPCM). …”
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Genome sequence and metabolic analysis of Pseudomonas fragi unveil the meat spoilage and CO2-antibacterial mechanism under high-oxygen modified atmosphere packaging by Jun Yang, Yikun Xu, Yimin Zhang, Rongrong Liang, Lixian Zhu, Yanwei Mao, Xin Luo, Xiaoyin Yang

“…Results showed that the strain P. fragi T1 was endued with spoilage-related genes associated with protease, lipase and esterase production, amino acid metabolism, carbon metabolism, sulfur metabolism, and putrescine metabolism, which was responsible for the hydrolysis of meat protein and lipid, as well as off-odor formation. …”
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Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes by Yan Dai, Meijia Huang, Yujuan Xu, Lixia Mu, Jingyan Gao, Hongbing Chen, Zhihua Wu, Anshu Yang, Yong Wu, Xin Li

“…Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation, which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa, and silkworm pupa were then hydrolyzed by alkaline protease. The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model, and the mice were immunized via intragastric gavage and intraperitoneal injection, respectively. …”
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Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae) by Julien Saguez, Anas Cherqui, Salima Lehraiki, Charles Vincent, Antony Beaujean, Lise Jouanin, Jean-Claude Laberche, Philippe Giordanengo

“…Overexpressed in transgenic plants, protease inhibitors showed insecticidal effects against several insect taxa. …”
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Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents by Rozina Ghulam Mustafa, Andleeb Dr Saiqa, Jorge Domínguez, Madiha Jamil, Saira Manzoor, Samna Wazir, Bushra Shaheen, Asma Parveen, Rida Khan, Shaukat Ali, Nazish Mazhar Ali, Fatima Jalal, Sadaf Azad Raja

“…Lipolytic, proteolytic, and amylolytic assays were done for lipase, protease, and amylase enzymes confirmation. In vitro anticoagulant effects were examined in the blood samples by measuring prothrombin time. …”
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Establishment of a bat lung organoid culture model for studying bat-derived infectious diseases by Mohamed Elbadawy, Nagisa Saito, Yuki Kato, Kimika Hayashi, Amira Abugomaa, Mio Kobayashi, Toshinori Yoshida, Makoto Shibutani, Masahiro Kaneda, Hideyuki Yamawaki, Kazuaki Sasaki, Tatsuya Usui, Tsutomu Omatsu

“…BLO expressed the entry receptors for coronavirus, Angiotensin-converting enzyme 2 (ACE2), and Transmembrane Protease Serine 2 (TMPRSS2), and alveolar type 2 cells were successfully sorted from BLO, which has an important role for the development of viral infection in the respiratory system. …”
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EMC2 suppresses ferroptosis via regulating TFRC in nasopharyngeal carcinoma by Xianghui Chen, Xiaoyan Wang, Yuxia Zou, Yan Wang, Tingting Duan, Zijie Zhou, Yi Huang, Qing Ye

“…Quantitative proteomics, protease inhibition, ubiquitin detection, and rescue experiments were performed to explore the mechanism of EMC2-regulated ferroptosis. …”
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Association of Circulating Neprilysin with BMI, Cardiovascular Health, and Kidney Function in High-Risk Pregnancies: A Pilot Study by Kaltrina Kutllovci Hasani, Azra Kulovic-Sissawo, Adam Saloň, Christina Stern, Karoline Mayer-Pickel, Mila Cervar-Zivkovic, Nandu Goswami, Herbert Fluhr, Ursula Hiden

“…Neprilysin (NEP), a protease involved in vascular regulation, has been linked to PE, but its role in endothelial function and vascular adaptation remains unclear. …”
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Drug-Drug Interactions Between HIV Antivirals and Concomitant Drugs in HIV Patients: What We Know and What We Need to Know by Emanuela De Bellis, Danilo Donnarumma, Adele Zarrella, Salvatore Maria Mazzeo, Annarita Pagano, Valentina Manzo, Ines Mazza, Francesco Sabbatino, Graziamaria Corbi, Pasquale Pagliano, Amelia Filippelli, Valeria Conti

“…Several studies have examined the pharmacokinetics and tolerability of different drug combinations. Protease inhibitors, followed by nonnucleoside reverse transcriptase inhibitors and integrase inhibitors have been recognized as the main players in DDIs with antivirals used to control co-infection, such as Hepatitis C virus, or with drugs commonly used to treat HIV comorbidities, such as lipid-lowering agents, proton pump inhibitors and anticancer drugs. …”
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Synergistic fermentation of vitamin B2 (riboflavin) bio-enriched soy milk: optimization and techno-functional characterization of next generation functional vegan foods by Ananya Rana, Neetu Kumra Taneja, Anupama Singh, Tejpal Dhewa, Vikram Kumar, Ankur Kumar, Komal Chauhan, Vijay Juneja, Harinder Singh Oberoi

“…Additionally, techno-functional characterization, including rheology and texture profile analysis, showed that enhanced protease activity of co-cultured LAB improved protein hydrolysis (6259 nm), positively impacting the water holding capacity (WHC) and overall acceptability of the fermented soymilk. …”
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Prevalence of Hyper virulence and Multidrug resistance Klebsiella pneumoniae isolates from UTI patients by Manar Abdul karim, Zainab Mohammed Alzubaidy, Khazal Dabua Wadi

“…Capsule production was also investigated using nigrosin stain followed by direct microscopy examination and it was found that 28 (93.3%) from K. pneumoniae isolates were surrounded by a capsule as well as (100%) biofilm formers. 86.6%, 77% and 70% of the isolates were siderophore, protease and coagulase were giving positive results respectively. …”
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Comparison of phenotypic and genetic traits of ESBL-producing UPEC strains causing recurrent or single episode UTI in postmenopausal women by Michelle Kalu, Peter Jorth, Annie Wong-Beringer

“…Virulence factors such as type 1 fimbriae adhesin FimH, the outer membrane protease OmpT, and the secreted pore-forming toxin α-hemolysin (HlyA) have been shown to support the formation of intracellular bacterial communities (IBCs) within bladder epithelial cells (BECs), facilitating persistence. …”
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Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab′)2-Based SPECT/CT Imaging by Dai Shi, Yiqiu Zhang, Zhan Xu, Zhan Si, Yuan Cheng, Dengfeng Cheng, Guobing Liu

“…Cet-F(ab′)2 was synthesized using immunoglobulin G-degrading enzyme of Streptococcus pyogenes (IdeS) protease and purified with protein A beads. The product and its in vitro stability in normal saline and 1% bovine serum albumin were analyzed with sodium dodecyl sulfate–polyacrylamide gel electrophoresis. …”
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Pan-cancer and experimental analyses reveal the immunotherapeutic significance of CST2 and its association with stomach adenocarcinoma proliferation and metastasis by Dan Huang, Jing Li, Zhijun He, Wenjing Liang, Likun Zhong, Jun Huang, Yinteng Wu, Shijian Zhao

“…PurposeCystatin 2 (CST2) is a cysteine protease inhibitor, and recent research suggests its potential involvement in cancer development. …”
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Bovine milk-derived sialylglycopeptide concentrate suppresses mumps virus infection by Tadanobu Takahashi, Yuuki Kurebayashi, Shunsuke Suzuki, Kobun Konagaya, Yutaka Narimichi, Eiji Kobatake, Hirofumi Fukudome, Toshiyuki Yamaguchi, Fumihiko Sakai, Toshinobu Arai, Toshihide Kabuki, Akira Minami, Hideyuki Takeuchi

“…We previously established an effective method for producing a milk-derived sialylglycopeptide (MSGP) concentrate from glycomacropeptide (GMP) in bovine milk using protease treatment. This MSGP concentrate was rich in short glycans containing α2–3 linked sialic acids, resembling the MuV binding receptors, which may contribute to inhibiting MuV infection. …”
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Phytochemical and Biological Investigations of Crude Extracts of <i>Astragalus pisidicus</i> by Esra Aydemir, Elif Odabaş Köse, Serap Özkaya Gül, Alaaddin Korkut, A. Cansu Kilit, Mehmet Engin Celep, Mustafa Yavuz, R. Süleyman Göktürk, Cengiz Sarikurkcu

“…Cell death was assessed using the WST-1 assay, while apoptosis was identified by colorimetric protease assay for caspase 2, -3, -6, -8, and -9, as well as cellular DNA fragmentation assay. …”
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