Research progress of protein haze in white wines by Zhaolong Liu, Le Xu, Jun Wang, Changqing Duan, Yanfeng Sun, Qingsen Kong, Fei He

“…Unstable wine proteins were usually pathogenesis-related (PR) proteins of grapes, mainly chitinases and thaumatin-like proteins (TLPs), which had lower isoelectric point (pI) and smaller molecular weight, and were highly resistant to the low pH values of wines and the protease hydrolysis during fermentation. At present, the technology of protein stabilization and clarification in white wines mainly included bentonite fining, heat treatment, enzymatic hydrolysis, polysaccharide treatment and ultrafiltration methods. …”
Get full text

Preparation of Xanthine Oxidase Inhibitory Peptide from Black Bean Protein by Enzymatic Hydrolysis and Its Uric Acid-Lowering Activity in Vitro by SUN Jingru, SUN Mingshuang, LÜ Wenqing, CAO Rong’an, DIAO Jingjing, WANG Changyuan

“…Based on XOD inhibitory activity and degree of hydrolysis (DH), various proteases were screened, and the hydrolysis parameters were optimized. …”
Get full text

G-cleave LC3B biosensor: monitoring autophagy and assessing resveratrol's synergistic impact on doxorubicin-induced apoptosis in breast cancer cells by Chiao-Chun Liao, Yuqing Long, Ming-Lin Tsai, Chun-Yu Lin, Kai-Wen Hsu, Chia-Hwa Lee

“…In this study, we developed a non-invasive autophagy detection system (NIADS-autophagy, also called G-cleave LC3B biosensor) by integrating a split-luciferase-based biosensor with an LC3B cleavage sequence, which swiftly identified classic autophagic triggers, such as Earle's Balanced Salt Solution and serum deprivation, through protease-mediated degradation pathways. The specificity of G-cleave LC3B biosensor was confirmed via CRISPR gene editing of pivotal autophagy regulator ATG4B, yielding diminished luciferase activity in MDA-MB-231 breast cancer cells. …”
Get full text

Evaluation of the effect of 48 weeks of BIC/F/TAF and DRV/c/F/TAF on platelet function in the context of rapid ART start by Akif A. Khawaja, Gary Whitlock, Sarah Fidler, Alfredo Soler-Carracedo, Merle Henderson, Graham P. Taylor, Marta Boffito, Michael Emerson

“…Despite differences in the demographics between treatment arms, the presence of an unboosted integrase inhibitor or boosted protease inhibitor in a test-and-treat setting did not impact platelet function. …”
Get full text

PAI-1: An Integrator of Cell Signaling and Migration by Ralf-Peter Czekay, Cynthia E. Wilkins-Port, Stephen P. Higgins, Jennifer Freytag, Jessica M. Overstreet, R. Matthew Klein, Craig E. Higgins, Rohan Samarakoon, Paul J. Higgins

“…It is now apparent that several proteases and protease inhibitors, most notably urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1), also interact with several cell surface receptors transducing intracellular signals that significantly affect both motile and proliferative programs. …”
Get full text

Antibodies against the multifaceted cathepsin D protein open new avenues for TNBC immunotherapy by Valérie Laurent-Matha, Pascal Roger, Séverine Guiu, Thierry Chardès, Emmanuelle Liaudet-Coopman, Pénélope Desroys du Roure, Timothée David, Aude Mallavialle

“…Therefore, immunotherapy, which has demonstrated its efficacy in different cancer types, could be a promising strategy for TNBC, given the limited therapeutic options currently available besides conventional chemotherapy. The aspartic protease cathepsin D (cath-D) is a tumor cell-associated extracellular protein with protumor activity, a marker of poor prognosis, and a target for antibody-based therapy in TNBC. …”
Get full text

Mechanistic studies on the effect of endogenous proteins on the starch retrogradation characteristics of corn before and after postharvest ripening by Nannan Hu, Weihua Qi, Jinying Zhu, Fuyin Zhao, Lin Xiu, Mingzhu Zheng, Jingsheng Liu

“…The corn starch-protein complexes before postharvest ripening (JD-0) and after postharvest ripening (JD-40) were subjected to protease treatment, and the influence of protein on starch retrogradation was studied. …”
Get full text

Quantifying LAGA mutated mouse IgG2a monoclonal antibody with a rapid pepsin digestion enabled immunoaffinity LC/MS/MS assay by Linlin Dong, Susan Chen, Konstantin Piatkov, Dong Wei, Mark G. Qian

“…To overcome these challenges, a trypsin alternative pepsin, an aspartic protease, was systematically investigated for its use in digesting the mutated mIgG2a antibody to allow generation of signature peptides for the bioanalytical quantification purpose. …”
Get full text

Developing an On‐Resin Synthesis of α‐Galactosylceramide‐Peptide Conjugate Vaccines by Dr. Michael A. Meijlink, Dr. Susanna T. S. Chan, Kathryn J. Farrand, Sarah L. Draper, Dr. Oliva K. Burn, Jasmine M. Seifert‐Simpson, Ngarangi C. Mason, Dr. Lisa M. Connor, Prof. Ian F. Hermans, Prof. Gavin F. Painter, Dr. Benjamin J. Compton

“…To facilitate this approach, novel analogues of αGalCer incorporating an activated ester spaced by an acid‐resistant, protease‐sensitive linker from either the 6′′‐position or the N‐acyl lipid were synthesised. …”
Get full text

Taxonomic and ecophysiological characteristics of actinobacteria in soils of the dry steppe zone of the Selenga Highlands (Western Transbaikalia) by E. P. Nikitina, L. B. Buyantueva, E. Yu. Abidueva, C. H. Sun

“…The examinations of extracellular enzymes showed that all isolates were capable of producing catalase and amylase; 78.6 % of the total number of isolates produced protease and lipase; 53.6 %, cellulase; and 28.6 %, urease. …”
Get full text

Modulation of iron metabolism by new chemicals interacting with the iron regulatory system by Yoshiaki Tsuji, Jun Ninomiya-Tsuji, Maurice Y.F. Shen, Benjamin R. DiFrancesco

“…Furthermore, we found that widely used haloketone protease inhibitors and acetamide herbicides inhibit the IRP-IRE system. …”
Get full text

Isolation and Evaluation of <i>Rhizopus arrhizus</i> Strains from Traditional Rice Wine Starters (<i>Jiuqu</i>): Enzyme Activities, Antioxidant Capacity, and Flavour Compounds by Bo Wan, Tian Tian, Ying Xiong, Siqi Wang, Xinyu Luo, Weifang Liao, Pulin Liu, Lihong Miao, Ruijie Gao

“…Significant variation in enzyme activities was observed, with acidic protease activity ranging from 280 to 1023 U/g, amylase from 557 to 1681 U/g, and esterase from 370 to 2949 U/g. …”
Get full text

N6-methyladenosine-modification of USP15 regulates chemotherapy resistance by inhibiting LGALS3 ubiquitin-mediated degradation via AKT/mTOR signaling activation pathway in hepatoce... by Ronghuan Fang, Zhigang Jia, Yuhang Xin, Kai Zhao, Wei Qin, Haoran Lu, Yi Zhou, Yongsheng Yang, He Fang

“…USP15 is a member of the ubiquitination-specific protease (USP) family, which can regulate protein ubiquitination, thereby affecting their stability, and is dysregulated in many cancers, but its expression and regulatory mechanism in HCC are unclear. …”
Get full text

Research advance of Bacillus velezensis: bioinformatics, characteristics, and applications by Ting Su, Biao Shen, Xingjuan Hu, Yue Teng, Peifang Weng, Zufang Wu, Lianliang Liu

“…Furthermore, based on its special properties, including high-yield protease production and high salt-tolerance, B. velezensis shows potential for use in marine protein fermentation, opening up new avenues for the development of novel food products and bioactive peptides. …”
Get full text

Nuciferine relieves type 2 diabetes mellitus via enhancing GLUT4 expression and translocation by Tongxi Zhou, Guanjun Song, Di Tian, Qinghua Liu, Jinhua Shen, Xinzhou Yang, Ping Zhao

“…A laser scanning confocal microscope was used to track the translocation of glucose transporter 4 (GLUT4) in L6 cells and the changes in intracellular Ca2+ levels in real time, and related protease inhibitors combined with western blotting were used to explore the mechanism of nuciferine. …”
Get full text

Classification of Nanomaterial Drug Delivery Systems for Inflammatory Bowel Disease by Wang H, Zhou F, Shen M, Ma R, Yu Q

“…In addition, NDDS can enable targeted delivery, increase local drug concentrations, improve efficacy, and reduce side effects, as well as protecting active drug molecules from immune recognition and protease degradation. The clinical use of these systems for treating IBD, however, requires further research. …”
Get full text

Effect of different feeding regimes in amur common carp fingerlings: compensatory growth, physio-metabolic responses, and expression of IGF-1 gene by Chowdhury Arka, Ghosh Tapas Kumar, Khatua Sanjib, Hore Sayani, Kumari Palak, Kajal Kumari, Jana Prasanta

“…T2/1 asserted superior digestive capacity in the starved group, with elevated (P < 0.05) protease and amylase activity. Reduced (P < 0.05) hepatic lactate dehydrogenase and malate dehydrogenase activity in T2/1 group suggests reduced stress, which might strengthen compensatory growth. …”
Get full text

ALDH2 Overexpression Alleviates High Glucose-Induced Cardiotoxicity by Inhibiting NLRP3 Inflammasome Activation by Ruiping Cao, Dian Fang, Jiahui Wang, Ying Yu, Hongwei Ye, Pinfang Kang, Zhenghong Li, Hongju Wang, Qin Gao

“…The results showed in high glucose condition that ALDH2 overexpression significantly increased H9C2 cardiac cell viability, increased mitochondrial ALDH2 activity and protein expression, and reduced mitochondrial reactive oxygen species (ROS) production, 4-hydroxynonenal (4-HNE), and lactate dehydrogenase (LDH) levels; meanwhile, the pyroptosis key components—NLRP3 inflammasome-related proteins, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), cysteine-containing aspartate specific protease 1 (Caspase-1), and interleukin-18 (IL-18) protein expressions—were significantly decreased, and IL-18 and interleukin-1β (IL-1β) levels were also decreased. …”
Get full text

Incidence of Hyperlipidemia among Adults Initiating Antiretroviral Therapy in the HIV Outpatient Study (HOPS), USA, 2007–2021 by Jun Li, Selom Agbobli-Nuwoaty, Frank J. Palella, Richard M. Novak, Ellen Tedaldi, Cynthia Mayer, Jonathan D. Mahnken, Qingjiang Hou, Kimberly Carlson, Angela M. Thompson-Paul, Marcus D. Durham, Kate Buchacz

“…Among 349 eligible ART-naïve PWH, 168 were prescribed INSTI-based ART (36 raltegravir (RAL), 51 dolutegravir (DTG), and 81 INSTI-others (elvitegravir and bictegravir)) and 181 non-INSTI-based ART, including 68 protease inhibitor (PI)-based ART. During a median follow-up of 1.4 years, hyperlipidemia rates were 12.8, 22.3, 22.7, 17.4, and 12.6 per 100 person years for RAL-, DTG-, INSTI-others-, non-INSTI-PI-, and non-INSTI-non-PI-based ART, respectively. …”
Get full text

Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury by Susan Bengs, Alexia Rossi, Martina Haberecker, Nidaa Mikail, Alexander Meisel, Achi Haider, Muriel Grämer, Angela Portmann, Atanas Todorov, Christof Schönenberger, Caroline E. Gebhard, Gabriela M. Kuster, Vera Regitz-Zagrosek, Catherine Gebhard

“…Abstract Previous work indicates that SARS-CoV-2 virus entry proteins angiotensin-converting enzyme 2 (ACE-2) and the cell surface transmembrane protease serine 2 (TMPRSS-2) are regulated by sex hormones. …”
Get full text