Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen by Ling Zhang, Xiaocui Yang, Kaijun Xiao, Yuyi Lu, Chunhai Li, Zijin Zhang

“…The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. …”
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Protein-ligand binding affinity prediction using multi-instance learning with docking structures by Hyojin Kim, Heesung Shim, Aditya Ranganath, Stewart He, Garrett Stevenson, Jonathan E. Allen

“…Our proposed method employs multi-instance learning with an attention network to predict binding affinity from a collection of docking poses.ResultsWe validate our method using multiple datasets, including PDBbind and compounds targeting the main protease of SARS-CoV-2. The results demonstrate that our method leveraging docking poses is competitive with other state-of-the-art inference models that depend on co-crystal structures.DiscussionThis method offers binding affinity prediction without requiring co-crystal structures, thereby increasing its applicability to protein targets lacking such data.…”
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Assessment of typing methods, virulence genes profile and antimicrobial susceptibility for clinical isolates of Proteus mirabilis by Lamiaa A. Salama, Hazem Hamed Saleh, Shaymaa H. Abdel-Rhman, Rasha Barwa, Ramadan Hassan

“…Different virulence factors as protease, urease and hemolysin production are detected. …”
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A multi-tissue longitudinal proteomics study to evaluate the suitability of post-mortem samples for pathophysiological research by Christian M. Beusch, Ken Braesch-Andersen, Ulrika Felldin, Pierre Sabatier, Anna Widgren, Jonas Bergquist, Karl-Henrik Grinnemo, Sergey Rodin

“…Additionally, we have conducted a comprehensive analysis of semi-tryptic peptides, revealing distinct consensus motifs for different organs, indicating organ-specific post-mortem protease activity. In conclusion, our findings emphasize the critical importance of considering PMI effects when designing proteomics studies, as these effects may significantly overshadow the impacts of diseases. …”
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IMMUNE THROMBOTIC THROMBOCYTOPENIC PURPURA: PATHOPHYSIOLOGY, DIAGNOSIS AND OPEN ISSUES. by Silvia Maria Trisolini, Alessandro Laganà, Saveria Capria

“… Immune thrombotic thrombocytopenic purpura (iTTP) is a life-threatening thrombotic microangiopathy characterized by microangiopathic hemolytic anemia, thrombocytopenia, and ischemic end organ injury due to microvascular platelet-rich thrombi. iTTP pathophysiology is based on a severe ADAMTS13 deficiency, the specific von Willebrand factor (vWF)-cleaving protease, due to anti-ADAMTS13 autoantibodies. Early diagnosis and treatment reduce the mortality. …”
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Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. …”
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Research Progress on Enzymatic Preparation and Functional Properties of Bioactive Peptides in Hairtail by Yuanyuan WU, Kewei ZHENG, Wenzhu LI, Cheng LI, Xiaojun YAN, Qingbao MA, Wei JIANG

“…The antioxidative activity of enzymatic hydrolysates is extensively studied, with alkaline protease and papain being the most commonly used enzymes. …”
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Potential of Microbial Diversity of Coastal Sand Dunes: Need for Exploration in Odisha Coast of India by Shubhransu Nayak, Satyaranjan Behera, Prasad Kumar Dash

“…Many neutrophilic and alkaliphilic eubacterial species, endophytic fungi from dunes have proved their ability for the production of extracellular enzymes like cellulase, pectinase, amylase, protease, tannase, chitinase, etc., which are of great importance to various industries. …”
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Decreased TNF Levels and Improved Retinal Ganglion Cell Survival in MMP-2 Null Mice Suggest a Role for MMP-2 as TNF Sheddase by Lies De Groef, Manuel Salinas-Navarro, Griet Van Imschoot, Claude Libert, Roosmarijn E. Vandenbroucke, Lieve Moons

“…Pathological conditions in the CNS are therefore often related to imbalanced MMP activities and disturbances of the complex MMP-dependent protease network. Likewise, in the retina, various studies in animal models and human patients suggested MMPs to be involved in glaucoma. …”
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Virtual Screening for Potential Inhibitors of NS3 Protein of Zika Virus by Maheswata Sahoo, Lingaraja Jena, Sangeeta Daf, Satish Kumar

“…In this study, we performed molecular docking analysis of these drugs against nonstructural 3 (NS3) protein of ZIKV. The protease activity of NS3 is necessary for viral replication and its prohibition could be considered as a strategy for treatment of ZIKV infection. …”
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TMPRSS2 as a Key Player in Viral Pathogenesis: Influenza and Coronaviruses by Gilmara Barros de Lima, Everton Nencioni, Fábio Thimoteo, Camila Perea, Rafaela Fuzaro Alves Pinto, Sergio Daishi Sasaki

“…TMPRSS2, a human transmembrane protease enzyme, plays a crucial role in the spread of certain viruses, including influenza and coronaviruses. …”
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Genomic Characterization of <i>Laodelphax striatellus</i> Permutotetra-like Virus and Self-Cleavage Function of Viral Capsid Protein by Jun Piao, Jiarui Zhang, Lujie Zhang, Jingai Piao, Haitao Wang, Yilin Xie, Shuo Li

“…Detection of purified CP in vitro showed that the cleavage could occur independent of any protease, confirming that CP has self-cleavage characteristics.…”
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Preparation of Cod Gelatin Antifreeze Peptides and Its Application of Frozen-Thawed Surimi by Jin ZHANG, Tao YUAN, Xiaomei SHA, Yuelun XU, Yating KUANG, Zizi HU

“…The results showed that the optimal preparation process for DcAFPs involved using compound protease, with a substrate concentration of 3.5% (w/v), an enzyme-to-substrate ratio of 5% (w/w), a hydrolysis pH of 8, a hydrolysis temperature of 55 ℃, and a hydrolysis time of 2 hours. …”
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Lysosome and Cytoskeleton Pathways Are Robustly Enriched in the Blood of Septic Patients: A Meta-Analysis of Transcriptomic Data by Jie Ma, Chuanxi Chen, Andreas S. Barth, Chris Cheadle, Xiangdong Guan, Li Gao

“…The key regulatory genes in the “Lysosome” pathway include lysosomal acid hydrolases (e.g., protease cathepsin A, D) as well as the major (LAMP1, 2) and minor (SORT1, LAPTM4B) membrane proteins. …”
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Targeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA by Xinmin Zhao, Xianghua Wu, Huijie Wang, Songtao Lai, Jialei Wang

“…Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. Ubiquitin‐specific protease (USP14) is involved in various pathological conditions including cancer; however, the role of USP14 in NSCLC remains elusive. …”
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The MMP-1/PAR-1 Axis Enhances Proliferation and Neuronal Differentiation of Adult Hippocampal Neural Progenitor Cells by Maria Maddalena Valente, Megan Allen, Valeria Bortolotto, Seung T. Lim, Katherine Conant, Mariagrazia Grilli

“…MMP substrates include protease-activated receptor-1 (PAR-1), a G-protein coupled receptor expressed in hippocampus. …”
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Incidence and Predictors of Antiretroviral Treatment Modification in HIV-Infected Adults: A Brazilian Historical Cohort from 2001 to 2010 by Letícia Penna Braga, Cássia Cristina Pinto Mendicino, Edna Afonso Reis, Ricardo Andrade Carmo, Cristiane Menezes de Pádua

“…Women (aHR = 1.62; p=0.022) and patients on protease inhibitor- (PI-) based regimens (aHR = 2.70; p<0.001) were at higher risk of ART modification.…”
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Improvement of Bacteriocin Production by Natural Zeolite and Detection of Antibacterial Activity of Leuconostoc carnosum Purified Carnosin by Haneen A. Ibrahim, Intesar N. Khelkal, Yusra M. Muhsin

“…The purified carnosin was characterized by determining the molecular weight using SDS PAGE (8KDa) in size .Carnosin had lost antimicrobial activity with different protease treatments (pepsin, trypsin and proteinase K). …”
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Early Delivery of Misfolded PrP from ER to Lysosomes by Autophagy by Constanza J. Cortes, Kefeng Qin, Eric M. Norstrom, William N. Green, Vytautas P. Bindokas, James A. Mastrianni

“…Additionally, 3-MA selectively impaired the degradation of insoluble Mut-PrP, resulting in an increase in protease-resistant PrP, whereas the induction of autophagy by rapamycin reduced it. …”
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Carbon Nanomaterials in the Treatment of Infectious Bone Defects and Wound Scars after Wushu Fractures by Shenghui Wei, Lei Zou, Xu Hu, Qiuming Li

“…In this paper, carbon nanofibers containing doxycycline, a small molecule protease inhibitor, were prepared by simple blending. …”
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